=(@m)=- Operation Manuals Microprocessor-Precision- Oxygen-meter Oxi 539 Matrix printer P 500 Oxygen sensor TriOxmatic® 300 Calibration beakers OxiCal®-Auto OxiCal®-SL Flow systems Oxi-Stirrer 300 + RZ 300 D201 WISSENSCHAFTLICH - TECHNISCHE WERKSTATTEN G.M.B.H. Drvrer.nat. K, Slevogt + D-82362 Weilheim + Tel (0881) | 83 - 0 + Tox 881 603 + Fax (0881) 6 25 39 BA OX1539-Sys/10/92/15 TM/MsiImportant hints for the user These notes must be read before the instrument is put into service! This instrument has been built and tested according to the German safety regulations for electronic test instruments, and left the factory in a technically and operationally perfect condition. In order to maintain this condition and guarantee safe operation the user must observe the references and warning statements . Before switching on the instrument, check that the mains voltage selected matches the mains supply voltage. ‘The proper function and operational safety of this instrumer only ifthe climatic conditions specified in the Technical Spe in this manual are adhered to. are guaranteed fications Opening of the instrument, adjustment, maintenance, repair and exchange of parts should only be carried out only by authorized personnel or by WTW. The instrument no longer confirms to the safety regulations and a dangerous ‘operation can be assumed when the instrument. ~ has been visually damaged = no longer functions = was stored for a long period under unsuitable conditions ~ was subjected to bad transport conditions. If itis obvious that the instrument is dangerous to persons then take it out of service and secure against any further inadvertent use. In case of doubt send the instrument back to the manufacturer “Wissenschaftlich-Technische-Werkstatten GmbH” for repair or maintenance,Contents list Important hints for the user 1 Operation manual for the Microprocessor-Precision- 5 ‘Oxygen-Meter Oxi 539 Recommended basic equipment for the oxygen measurement 6 Display, Keys and Connections 7 Front view 7 Rear connections 10 Putting into operation WW Calibration and measurement 12 Calibration with OxiCal®-SL 12 Measurement of oxygen concentration resp. oxygen saturation 12 Automatic selection of stable readings (Auto-Read) 13 Measurement of the oxygen concentration with salinity correction 14 Table of calibration procedures 15 Calibration with an air calibration beaker 18 Automatic precision calibration with OxiCal®-Auto 16 Calibration in air saturated water 17 Calibration for oxygen-saturation measurement in air 17 Calibration against 2 known (titrated) concentration value 18 Fast run for parameter adjustment 19 Time, date, and print period time 20 Set time, date and print period for the printer 20 Read time, date and print period 21 Printer operation 22 Connections 22 Functions 22 Activation of the time controlled printout 25Computer connection, OXI 539 RS only ‘Commands Operation Program example for IBM compatible computers (GWBasic) Error messages Technical data Oxi 539 User's instructions for the Dot printer P 500 Instructions Dip-Switch positions for the printer P 3000 User's instructions for oxygen sensor TriOxmatic® 300 Assembly of oxygen sensor TriOxmatic® 300 Operation Maintenance Recommended accessories Maintenance and spare parts Technical data User's instructions Calibration beakers OxiCal®-Auto; OxiCal®-SL Hints for the calibration with OxiCal®-systems Preparation of calibration with OxiCal®-systems Maintenance OxiCaP-SL Design of OxiCal®-Auto Maintenance OxiCal?-Auto User's instructions Flow-Systems - Stirrer (Magne Assembly of the WTW-flow system with stirrer ‘Assembly of the WTW-flow system with flow-through accessory Technical data stirrer Oxi-Stirrer 300 + Stirring acc. RZ 300) - Flow-through measurement (Flow-through accessory D 201) 26 26 27 2B 29 31 37 40 oT 42 42 43 46 47 48 49 50 50 51 51 51 53 54, 355 56=G@im)= Operation Manual Microprocessor-Precision- Oxygen-Meter Oxi 539 Thank you for choosing the Oxi 539; a highly developed microprocessor-controlled oxygen meter that will satisfy all your requirements. This manual provides an overview of the many functions of the instrument and supports the user with detailed information.The many diagrams and illustrations describe the stages of operation clearly but the setting up and measured values shown are obviously only examples. Our technical customer advisory service is available for general questions about oxygen measuring techniques.We will gladly send the application reports described on request. WISSENSCHAFTLICH - TECHNISCHE WERKSTATTEN G.M.B.H. Dr.rer:nat. K. Slevogt » D-82362 Weilheim + Tel (0881) 1 83 - 0 + Tox 881 803 + Fax (0881) 6 25 39 BA 014539/2193/1; TMMaiRecommended basic equipment for oxygen measurement. Oxygen Meter Oxi 539 Oxygen Sensor TriOxmatic® 300 BOD bottle KF 12 Stirring accessory RZ 300 Magnetic Oxi-Stirrer 300 Calibration beaker OxiCal® SL AaronDisplay, Keys and Connections Front view O Cal OxiCal O Oo 3 4 1 Display 1: Oxygen readings, user's instructions, data (e.g. time) 2. Display 2: Temperature of measuring solution, Salinity (=salt contents), solubility factor, user's instructions, data (e.g. time) 3 Status display @ cat : Calibration in air saturated water + Calibration for measurement in air Status LED illuminated + Calibration in OxiCal® vt a “@: ‘Status LED flashes + Automatic determination of readings b @ ae, © Status LED not ® illuminated + Salinity correction switched on 84 Foil keys Scroll mode: Depress and hold the key until the required function is set. Red lettering: Call-up of second function : Second funetion of the keys (2) Gold lettering: Readings and constants + Selection of calibration procedure (Seroll mode) + Calibration against a known concentration value Set solubilty factor : Measure oxygen and temperature, select concentration or saturation display (Scroll mode) : Display of the relative slope S Switch ON/OFF salinity correction : Calibration against a known concentration value Set salinity : Automatic determination of readings - switch on/offWhite lettering: Adjustment and confirmation : Start calibrations and measurements (Auto Read) , Acknowledge inputs, fast run on/off + Set the higher numerical values, Switch on printer function. + Set the lower numerical values, Switch off printer function. Green lettering: Printer and time Print the readings + Set the printer function + Initiate a line feed : Display date, time and print interval (scroll mode) + Set the time, date and print intervalRear connections Maden Gemory — Whsanschatiih-lechnische Werstétten, 82362 Walhakn i 8 1 Mains supply switch 2 Oxi539__ : WTW-specifc interface for printer P 500 (Oxi 539 RS : V24/RS 232 C-interface for computer or printer 3. Temperature voltage output for laboratory recorder 4 Oxygen concentration resp. saturation voltage for laboratory recorder 5 Connection for oxygen sensor TriOxmatie® 300 : Spin plug Hloctrode 6 Connection for calibration beaker OxiCal® Auto, :7-pin plug 10Putting into operation + Connect the instrument to the mains supply ‘+ Connect the oxygen sensor TriOxmatic® 300 + Connect OxiCal® ~Auto (if available) ‘+ Switch on the instrument Proposals for familiarisation with the instrument Initial set-up: Depress key and switch on simultaneously Release the key at display test, + Wait in display test until a signal sounds + Wait for polarisation time of the sensor (25 to50 minutes) The Oxi 539 is now ready for operation and set to the operating mode or to the initial set-up mode. 11Calibration and Measurement Calibration with OxiCal®-SL Dry the oxygen sensor. Put the sensor approx. 15 minutes before calibration into OxiCal-SL. Close cap (Ensures temperature equalization between sensor and OxiCal®-SL)! OxiCal @ eeteett ; u relative slope temperature until Start calibration Jab waitfor acouste signal am determined relative slope S Calibration is finished, the determined data are stored. (0,6 to 1,25 = admissible range for S) Measurement of oxygen concentration resp. oxygen saturation Immerse oxygen sensor into sample solution! Care for incident flow! CC) e.. Ceo Concentration Temperature Wigs’ Let Relative oxygen saturation Temperature index (9-saturation) \l —@ / Z\N ss General notes on oxygen measurement: WIW-OX\-Fibula 12Automatic selection of stable readings (Auto Read) Reading frozen Switch on auto range selection Start Actual reading measurement [| 2b4]° ws ‘a! Waite for acoustic signal Reading frozen Layg|* [269)° Actual reading Switch off auto range selection Instrument measures continuously The Oxi 539 determines automatically the stability of the sensor signal: Meas. period: Minimum 14 seconds; Maximum 3 minutes Max. Drift: B < 0,05 mg 0,/1/7 s,0 < 0,05 K/7s ge, to Figad 0 it ‘Read 13Measurement of the oxygen concentration with salinity correction The oxygen solubility depends on the salinity of the sample. This should be respected for concentration measurements in salt-bearing water. Setting of sali y A For seawater only resp. mainly NaCl-bearing water! e@ C554» [Ooo (©,-concentration Salinity [EO nn O,-concentration | Sal Set salinity Measurement of the oxygen concentration in relation to the salinity CS 9am» Cem” Set concentration measurement " @ I 7 mm LoS Switch on salinity ©, concentration Temperature j correction ‘corrected Oo ~~ Ceol : Switch off salinity ©,-concentration ‘Temperature correction ot corrected ONIOFF in mode "mgt" only. %emeas. value is not related to salinity. 14n procedures OxiCal-Auto —OxiCal @ in air saturated @ Cal automatic precision water calibration constant meas. condition because of electronic calibration-, dewing- and temperature control. Cal || ‘against a known concentration value OxiCal®-sL. OxiCal @ Standard (roa) [eo] Sotubi et i 53] soniye Calibration an air calibration beaker Switch on the instrument with connected sensor approx. 15 minutes before calibration (polarisation). Dry oxygen sensor and put into calibration beaker. Close turning cap (equalization of temperature between sensor and air calibration beaker)! Oxical @ 2 (ee) cee j Slope Temperature i Road Start calibration J ‘a’ Wait for acoustic signal OxiCal @ Calibration is finished, the data are stored. Determined slope 15Automatic precision calibration with OxiCal?-Aute Connect OxiCal®-Auto to Oxi 539 Approx |5 minutes before calibration switch on the instrument with connected sensor (polarisation), dry oxygen sensor, put into OxiCal®-Auto, Close turning cap (equalization of temperature sensor and OxiCal®-Auto)! OxiCal @ wat [FAL OxiCal @ Start calibration E i a) a =O) co im] ug8s [ <2 16]° Slope S Temperature ‘al Wait for acousti signal ugil Determined slope H g 2 coe The calibration is finished, the data are stored. Take sensor out of OxiCal®-Auto - ready to measure Hint: After starting automatic calibrations are performed as long as the sensor remains in the OxiCal®-Auto. For better control of the slope (preparation of the sensor) the first 20 calibrations are performed in intervals of 1 minute; then in intervals of 30 minutes. After initiation (prox. 90 minutes) the automatic slope control of long term stability starts. 16Immerse oxygen sensor into air saturated water! Care for flow! until Slope Temperature Start calibration ado wate for acoustic signal Cal MH oe Determined slope The calibration is finished, the data are stored, Calibration for oxygen saturation measurement in air dry sensor and put sensor into OxiCal-SL or -Auto (disconnect OxiCal®-Auto from the instrument)! [2 76]° Slope Temperature Start calibration Ja Wate for acoustic signal Used slope The calibration is finished, the data for measurement in air are stored. For measurement in air only the operation mode "%S" is admissible! Display 100 %-saturation corresponds to an oxygen share of the air of 20,95 % Hint for calibration: The diffusion of the oxygen through the sensor membrane in liquid media (water) doesn't have the same behaviour as in gaseous media (air). This is respected during the calibration: That is why that all ‘OxiCal-calibration procedures (sensor in air calibrates for measurement in water) use a temperature dependent saturation value of >101 %; for measurement and calibration in the same media the calibration procedure "Cal" (adjustment to 100 %) should be selected 17Calibration against a known (titrated) concentration value Adjustment via a relative solubility factor (for all solutions) no conforming ‘temperature correction; so measurement is allowed at calibration temperature in solutions of same compound only. Immerse sensor into solution Care for flow! known oxygen concentration? nw LIG00) ©,-concentration Solubility factor 7 A nol TVS ©,-concentration Solubility factor Set O,- concentration If solubility factor + 1,0 - "mgfl" is flashing A The adjusted solubility factor remains active even after switching off the instrument! Fast run ON/OFF (refer to chapter "Fast Run") storage and end the adjustment the solvents, the dissolved agents and the temperature T. Oxygen partial pressure pO, and T are measured. The concentration is calculated accor- ding to the solubility function for pure water (resp. seawater) stored in the instrument, The solubility factor indicates the relative concentra change to pure water (resp. seawater). Except of pure water the displayed concentration value is valid for solutions of the same compound at calibra- tion temperature only. 18Fast run for parameter adjustment For setting of salinity, oxygen concentration and solubility factor e (©,-concentration Salinity bed z 1. | sai = ca Modifications start with the pana last decimal point Run ewrea Switch on fast run 4B tno Cad5|n © Modifications start with the penultimate decimal point. The last decimal point remains unchanged Switch off "Fast Run" store and end the adjustment 19Time, date, and print period for the printer Set time, date and print period for the printer [GLUE] rm [Z| Hour. Minute. Seconds @ Hour © Minute @ Second Lise joee (ES TN Hour. Minute. Second Print period time: Status display "Time" is flashing. @ Mine @ Hour @ Second Leave the setting mode Set the flashing digit Set the fiashing segment Set the flashing digit (Gs... 24h) 20Read time, date and print period Hour. Minute. Second Day. Monat. Year Hour, Minute. Second Time interval: Funktion indicator "Time" flashes. aiPrinter operation Connections Instrument version: Oxi $39, "Printer" interface WTW-Printer P 500 Instrument version: Oxi 539 RS, "539 RS" interface Printer with RS 232 C serial interface and IBM character set Functions gle printout Printout of the readings Paper Line feed ‘One line feed = Depress key once Several line feeds = Hold key depressed Functions On Functions Off Event controlled print out Log printout only after setting the printer functions, calibration and Auto Read 22‘Time controlled printout (Timer Function) Time controlled printout of values Time interval: refer to "setting print period for the printer” (page 20) Date and time with single printout (Date/Time) Logs of time or event controlled printouts always start with date and time 23Reset line numbering to 1 (Set Line Number) If display shows Stn | reset to 1: Time controlled printout: start with in Single printout: confirm "SLn On" with Line numbering is continous up to 999, resets to { if power is removed. end with 24Activation of time controlled printout ae Switch on time controlled printout Start Stop 25Computer connection, Oxi 539 RS only Pin connections Computer [Printer with RSZ32| 9 Pin 25 Pin [interface RxD2 [3 TXD2 | TxD ) Oxi 539 ban TXD3 [2 RxD3 | RxD (@) ) 6), ea) C // [ors Je ox |. \) zs sos |5 sc 7 | sc \ o 2 ©) (3) O By ve Rts7 | CTS 5 iE \ CTS8 | 7 RTS 4 | DTR whennoe A aaisil, short ect CTS nd RTS V24RS 232 C interface (rear side of instrument) Commands Command Function = Start communication with the first Carriage Return (CHR$(13)) "CRUN" — ~ Starts operating sequences or continues interrupted operations "CLOC" _ - Reverts the Oxi 539 to normal operation; end of communication Calibration "CCA1" — - Calibration in air saturated water "CCA2" —- Calibration in OxiCal®-calibration beakers Measure oxygen and temperature "CCON" —- Oxygen concentration measurement "CSAT" —- Oxygen saturation measurement "CARD" — - Auto-Read- and measurement "ON/OFF" Switch on salinity ‘only admitted after command "CCON" 2ugelassen "CSAL1" switch on salinity correction "CSALO" - switch off salinity correction 26Operation Computer Oxi 539 Start of transfer v Open serial port v Send Carriage Return "OXI539. >" received Keyboard inhibited v ‘Transfer: Send commands t Receive values with dimensions and texts Terminator: "OXI539 >" XN 4 Process commands End of transfer V Send "CLOC" "OXI539 >" received Close file \ 4 Keyboard enabled Software for IBM compatible computers, eg.: TELEMATE V2.1 Terminal prograrn for the simple operation of the oxygen meter, avallable from: Microserve, Postfach 1368, W-2804 Lilienthal PRINT DAT V.1 Sample program for the data transfer triggered by test instrument, available from: Wissenschaftlich- Technische Werkstatten GmbH, Dr.-Karl-Slevogt-Str. 1, D-82362 Weilheimn 27Program example for IBM compatible computers (GW Basic) 10 OPEN "COM1:1200,n,8,1" AS #1 100 REM Enter command and send BES 120 IF BES = "e" THEN CLOSE ##1:END 110 PRINT : INPUT "Comman 130 PRINT#1, BE$: GOSUB 1000 140 GOTO 110 1000 REM 1010 Ys="" 1020 INPUT#H1.X$ -Recelve data and output 1030 YS=Y$+X$+"," 1040 IF Xs "OXI539 >" THEN PRINT "Received data: “Y$:RETURN 1050 GOTO 1020 line 10 + openss the interface line 110.140: The instrument can receive commands after the first "Carriage Return has been transferred. Any previous characters have no effect. The "e” and "Return keys end the program. line 1010... 1050: Reads the data sent from the instrument and displays them on the screen. ‘Output format: - ASCII characters (IBM code) - Character strings consist of one or more fields - Fields are separated with a comma - Text, values or dimensions are shown in one field ~ A dimension is always given with a value 28Error messages Display Cause Sensor not connected to Oxi 539 Cc 24g ‘Oxygen concentration or -saturation range exceeded (> 300 mg/l resp. > 999 5) 6. OFL Temperature of meas. solution < -5 °C or > +60 °C. No stable reading (Auto Read) EF Undue sensor slope (S > 1,25 or $< 0,60) EY mal A i Sensor colder than OxiCal®-Auto Too high temperature difference between ‘OxiCal®-Auto and sensor ‘Auto Reg signal electrolyte solution used up moh Action Connect sensor or control connection Control measuring solution and sensor ‘Temper measuring solution between 0°C and + 50°C (= application range of the sensor) . Care for constant flow. Exchange electrolyte solution and membrane head of the sensor; eventually clean cathode (refer to operation manual: TriOxmatic® 300). Dry sensor and membrane; wait for temperature equalization between OxiCal®-Auto and sensor. Wait for temperature equalization between OxICal®-Auto and sensor. Exchange electrolyte solution and membrane head of the sensors; or clean cathode (refer to operation manual: TriOxmatic® 300). Measurement can be finished. 29Sensor system untight; membrane head not correctly screwed. EG No reproducable sensor signal during calibrations in OxiCal®-Auto mg JHE Defective electronic part or loss of internal ‘compensating and calibrating data Tighten membrane head; exchange electrolyte solution and membrane head of the sensor; or clean cathode (refer to operation manual: TriOxmatic® 300). Pall sensor out of OxiCal®-Auto. Care for stable ambient conditions (solar radiation, draft) for OxiCal®-Auto => further more E8 => exchange membrane head of the sensor trument to the normal mode (see 10 operation). If "DATA LOST" reoccurs, the instrument must undergo repair at WTW. RS 232 C interface Cause INVALID COMMAND" Undefined command. "OFL" in character string ‘Oxygen concentration or -saturation indication range is exceeded > 300 mg/l resp. > 999 %).. ‘Temperature < -5 *C resp. > 60 °C. "E2"."E8" in character string Action ‘Check the syntax and enter command again (Check measuring solution and sensor. ‘Temper measuring solution between 0°C and + 50°C (= Application range of the sensor) . See error messages "Display" Voltage outputs Rec (O,) = 2050 mV Rec (T) = 2050 mv Rec (,) = 0 mV and Rec (T) = O mV Range exceeding resp. error €2, £6, E7 ‘Temperature of the measuring solution < -5°C resp. > +60°C All not measuring operation modes, at calibration and errors E3, E4, E5, EB 30Technical data Oxi 539 Parameters, Ranges, Accuracy Ranges of adjustment Barometric pressure compensation Display Analog In/Ourputs Oxygen concentration: 0,00 .. 29,99 mg/l 30,0 .. 299,9 mall ‘automatic range switch £0,5% of meas. value + 1 digit 0,0 .. 299,9% 300.. 999 % £0,5 % of meas. value + 1 digit ‘Oxygen saturation: Temperature: - 50°C. + 60°C 201K Salinity: 0,0.. 99,9 Solubility factor: 0,100 .. 9,999 automatically from 800. 1100 mbar Display 1 4-digit LED, red, 7 segments, 14 mm high Luminous fields for mg/l, % Display 2: 4-digit LED, red, 7 segments, 14 mm high Luminous fields for *C, Sal Luminous fields for "Date" and “Time” Status display: LED red 8 - pin diode socket for TriOxmatic®-300 7 - pin diode socket for OxiCal®-Auto : ©: A NTCi 1 NTC3 2 NTC2 2 LED anode 3 NTC1; NTC2 3 NTC3 4 Anode 4 LED cathode 5 Cathode 5 Phototr.-collector 6 Reference electrode 6 Phototr.-emitter_ 7 Shielding 7 Code contact 8 Auxiliary electrode Recorder outputs: ©,- 4mm banana sockets "C - 4mm banana sockets 31Recorder output Digital interfaces Clock and date Power supply Power consumption meas. range potential Oxygen concentration | 0,00... 29,99 mg/l | 66,6 mV/ (mg!) 80,0 .. 289,9 mgll | 6,66 mV/ (mg!) Oxygen saturation 0,0...299,9% | 6,66mVv/% 900... 999% | 0,666 mv/ % tomv/K Temperature 50 (-50..+600mV) 480,00 Oxi 529: Printer, 15 pin for plug-on WTW-printer P 500 (Oxi $39 RS: V24l RS 232 C, 9 pin for computer or printer, galvanically isolated| Baud rate: 1200 Data bits: 8 Stop bit: 1 Parity: no parity Handshake: Hardware Max. cable length: 15 m Display format: ASCII character string, IBM character code, separation by comma Battery: Uthiumn 1.7 Ab, Life: 10 years minimum Accuracy: Oi%e 230 V/ 50 Hz (-15%/+6%) optionally 115 V / 60 Hz (-1 5%-+6%) 10VA 32Data protection Housing Eletromagnetic compatibility (EMC) see test certificates Dimensions/Weight All manual settings are stored for an unlimited period when the instrument is switched off or at a power supply failure. After switching on the instrument switches automatically to the last selected operation mode. Degrees of protection: IP 40 (1EC 529, EN 60529, DIN VDE 0470) IP S4 (IEC 529, EN 60529, DIN VDE 0470) for keyboard {Insulation class: II (DIN VDE 041 | according to IEC 348) Climate class: 2 (VDINVDE 3540) Ambient temperature: Storage: -25°Cto +65°C Operation: +5°C to +40°C relative humidity: Yearly mean: $75% 30 dayslyear: 95% remaining days: 85 % light dew: yes Emission: Class B — (ENS5022) Class A (FCC) lornunity ESD (IEC 801-2) Test security levels RF (IEC 801-3) see test certificates BURST (IEC 801-4) 270 x 120 x 220 mm / approx. 1.8 kg 33Test certificate: Electromagnetic compatibility EMC SENTON SE tenn MEMI MC Toeoer corincine q EXPERT OPINION (Radio Frequency Interference Test) fr Kind of product: Oxigen Meter ype OXI 539 Manufacturer WTW Gnblit Applicant: WIW Guill Date effected ‘September 18, 1992 We hereby certify, thtt the above mentioned model has been tested and found to bs incompliace wth the following regulation(s): EN 55022 Limit ClassB CC Part 8, Subpart Limit Class A TEC 801-2 Test Severiy Level 3 IEC 801-3 Test Severity Level 2 TEC 91-4 Test Severiy Level 3 Remarks: All tests ave been performed with a P 500 printer connected to the OXI 539. Senton GmbH Za SINTON Gr Bnoanin Annu Fp: Hig: ¢021/208 eyTest certificate: Electromagnetic compatibility EMC SENTION — OAV. pahentam AUDA Toca CURTIFICATE 4 EXPERT OPINION (Radio Frequency Interference Test) for Kind of product: Oxigen Meter ‘Type OXI 539 RS Manufacturer : WTW Gmbit Applicant’: WIW Gmbit Date effected : September 18, 1992 We hereby certify, thatthe above mentioned model has been tested and found to be in compliance with the following regulation(s): ‘EN $5022 Limit Class B CC Part 15, Subpart B Limit Class A IEC 801-2 Test Severity Level 2 TEC 801-3 Test Severity Level 2 TEC 801-4 Test Severity Level 2 Senton GmbH Johann Roidt STON Gn: Clare Anne Futian WEA Sra 9421/4208) C€92 35=GQrm)= User's instructions Dot printer P 500 The P 500 is a mini printer used with the WTW measuring instruments via a special printer interface. The printer can only be used in conjunction with the instrument.The printer functions are dependent upon and can be controlled from the instrument (see the measuring instrument instructions). press here to open the printer cover WISSENSCHAFTLICH - TECHNISCHE WERKSTATTEN G.M.B.H. Dr.rer.nat. K. Slevogt + D-82362 Wellheim + Tel (0881} 1 83 - 0 + Ttx 881 803 + Fax (0861) 6 25 39 BA P500i5/92/1 37Attention: Piug the printer into the interface only when the instrument is switched off Fitting a new paper roll = Open the printer = Remove the empty cardboard roller (a) - Open the new roll of paper and fit «Insert the start of the roll in the slot (b) - Depress the "Paper Feed” key on the instrument until approx. 5 cm of paper is shown (c) - Feed the paper through the slit in the cover when closing ‘Changing the print ribbon = Open the printer - Depress PUSH (d) and remove the ribbon - Tension the new ribbon with the knob (e) - Insert the paper between the ribbon and the cassette housing - Insert the printer ribbon ~ Pull in the ribbon by turning the knob (e) - Feed the paper through the slit in the cover when closing 38Technical Specifications P 500 Printing method Print format Printing speed ‘Character format Ribbon Paper Dimensions Weight 7 dot printer dots per line: 144 characters per line: 24 approx. 150 ms!t-dot line approx. 0.7 lines/sec character size 17 (8) x 24 (H) mm distance between dots horizontal: 0.33 mm vertical: 0,33 mm Model FBK 500 Model RP 500 wood-tree paper width: 58 mm coll diameter: max. 30 mm 100 x 70 x 100 mm approx. 0.25 kg 39Instructions Dip-Switch positions for the Printer P 3000 Configurate Dip-switch for WTW-instruments 1. Switch off printer 2. Open cover of Dip-switch 3. Set switch position 4, Close cover 5. Switch on printer Interface configuration for printer with RS 232 C/V 24 Interface 1200 baud; 8 bit; parity none; parity odd 40=G@rm)= User's instructions Oxygen sensor TriOxmatic® 300 ual WISSENSCHAFTLICH - TECHNISCHE WERKSTATTEN G.M.B.H. Drsrer.nat. K. Slevogt * D-82362 Weilhieim + Tel (0881) 1 83 - 0 + Tex 881 803 + Fax (0881) 6 25 39 BA TeiOxmatic300/9192/1; TM/Mai 4Assembly of oxygen sensor TriOxmatic® 300 Shaft Thermistors aE \ ake Membrane head WP 90 x eae electrode i |tetheaey Counter electrode | | | \ | | \ ! 1 | \ | 1 VI I : \ | Isolator I Operation \ a Reference electrode Polarisation ‘After switching on the instrument the oxygen sensor polarises. For first putting into operation or after cleaning the sensor is ready for operation after 25 or 50 minutes (the membrane head must be free of air bubbles). By calibration with OxiCal®-Auto permanent measurement and comparisons enable a calibration at the right time and as fast as possible. Calibration Refer to user's instructions in the manual. Life time per filling At permanent use and 100 % oxygen saturation the electrolyte solution is sufficient for approx. 550 days. The end of the life time is displayed by the instrument. The electrolyte is used up and must be renewed. A premature failure by damage of the membrane will be signaled, After life time the membrane head must be exchanged. 42Storage Store the sensor In the OxiCal®-beaker. Maintenance WTW delivers the sensor ready to use. A refiling is absolutely necessary after service life (AutoRez) or in case of interferences of the measuring function (leakage; contamination). A Disconnect sensor from instrument during maintenance works! Refilling and regeneration Disconnect sensor from Unscrew membrane head. Rinse sensor head with H,O instrument. @® Cleaning of the electrodes A cleaning is necessary, in case of - short service life (contamination) - silver coating of the working electrode (gold-cathode) - slope of the sensor falls short or exceeds and cannot, be corrected by exchanging the membrane head, Clean and dry counter electrode carefully with a After cleaning continue with point (6) paper towel 43Wet the membrane with some Fill membrane head with drops of electrolyte solution. _—_ electrolyte solution. bubbles. inse sensor with electrolyte Put sensor vertically into Screw on membrane head, solution. membrane head. superfluous electrolyte solution is removed. Air bubbles at the gold cathode: bubble-tree. - refilling will be necessary. ‘Small bubbles at the edge of the membrane head have no negative effect. 44Cleaning of the electrodes Cleaning of the electrodes is not often required and should not be done with each refilling! Dark patches on the counter electrode need no cleaning. Cleaning is only necessary in case of ~ short service life (contamination) - silver coating of the working electrode (gold cathode) ~ the slope of the sensor falls short or exceeds and cannot be corrected by exchanging the membrane head. Never treat the reference electrode mechanically with cleaning solution! In case of contact with cleaning solution “> Rinse electrode with distilled water and run the sensor (with membrane head) in air for several days. Cleaning of the counter electrode Immerse counter electrode Take care that reference Clean counter electrode into cleaning solution for electrode doesn't get into with paper towel approx. 2 hours. contact with cleaning solution. Water counter electrode for at Rinse sensor head with least 10 min. in distilled water. distilled water. 45Cleaning of the working electrode (cathode) A In case of silver coatings only! Wet electrode with one dro} Remove carefully silver of water. coating with abrasive paper. Recommended accessories Stirring accessory in connection with Oxi-Stirrer 300 for constant, definined flow to the sensor Funnel set for BOD-determination in Winkler-bottles BOD bottle for determination of BOD Flow through accessory for oxygen measurements in pressureless operation at flow-through ‘quantity between 25 and 65 mllmin, Rinse with distilled water. ~ Polish working electrode in wet condition only! ~ Use WTW-abrasive paper only! ~ Donot use any other abrasive paper or fibre glass pent Model RZ 300 Ts 19 KF 12 D201 Ono. 203 824 205 710 205 700 203 730 46Maintenance and spare parts Exchange membrane heads (3 pleces) Electrolyte solution Foaing ring Cleaning solution for silver counter electrode Abrasive paper ‘OxiCal®-calibration beaker Accessory kit Model WP 90/3 ELYIN FRI9 RL-AGIOXI SF 300 OxiCal®-sL ZBK 90/190 O-ne. 202 725 205 210 205 712 205 200 203 680 205 362 202.410 47Measuring principle ‘Temperature compensation ‘Signal analysis Response time at 20 °C Min, flow ‘Own consumption of oxygen at 20°C. Slope at 20 °C Polarisation voltage Drift Lifetime per filing Max. pressure Sample temperature Storage temperature Material: Dimensions: Technical data Oxygen Sensor TriOxmatic® 300 Membrane covered amperometric sensor with potentiostatic working method (TriOxmatic® three electrode system) System for detection of membrane leakage IMT-compensation 0,1-% (0,01 mail) '90 % of end value display) 12 s 2. (55 % of end value dapley) [8s ty (99 % of end value display) 55 s > 3 cmls with 10% measurement accuracy 10 cmfs with 5% measurement accuracy 18 cm/s with 1% measurement accuracy 0,009 ig hr! (mel) 1,33 nA’mbar -750 mV < 0,2 %/d at permanent polarisation 550 days 6 bar 0°... 50°C =5°C..50°C Membrane head POM Membrane FEP Sensor head POM Thermistor housing VA-steel 1.4571 Shaft Epoxy Closing cap Pvc Shaft length 160 mm Shaft diameter 15,5. mm Membrane thickness 13 jum Cable length Som 48=@nm)=- User's instructions Calibration beakers OxiCal® - Auto For precision calibration of WTW-Oxygen sensors WISSENSCHAFTLICH - TECHNISCHE WERKSTATTEN G.M.B.H. Drarersnat. K. Slevogt + D-82362 Weil Tel (088!) 1 83-0 + Trx 881 803 + Fax (0881) 6 25 39 BA OxiCal-SL/Auto /10/92/1; TM/Mai 49Hints for the calibration with OxiCal®-systems Increase the calibration accuracy by avoidance of high temperature effects to the OxiCal®-beaker! (range of application 15°C to 40°C) High temperature variations. In case of high temperature differences between sensor and OxiCal®: Accelerate the temperature equalization by immersion of the sensor into a water bath! Calibrate the sensor in the measuring condition! (with or without stirring accessory RZ 300)! In case of mechanical strain of the membrane head of the sensor (e.g: removal of the RZ 300) a new calibration is recommended. Preparation of calibration with OxiCal®-system Insert sensor as far as possible. Tighten cap. Start calibration at the instrument. 50OxiCal®- SL standard calibration beaker consisted in the sensor equipment Maintenance OxiCal®-SL ‘Sponge in the OxiCal®-SL should always be kept moist (never wet)! ‘Open cap. Moisten sponge. Close cap. OxiCal®- Auto automatic precision calibration beaker Design of OxiCal®-Auto Screw eap Sealing Shaft. Protection Light barrier Thermistor — Evaporator screw cap Evaporator NZS7— Base plate Cooling plate 7 cect 51Maintenance OxiCal®-Auto Fill the evaporator approx. every | or 2 monthst Check fiting level ill with defonised water (max. 5 ml/ min. 1 ml) Close evaporator. Screw evaporator into OxiCal®, 52=Crmp)= User's instructions For optimum and reproducible flow of WTW-oxygen sensors. Flow Systems with stirrer flow-through or measurement Stirring accessory Flow-through accessory D 201 WISSENSCHAFTLICH - TECHNISCHE WERKSTATTEN G.M.B.H. Dr.rer.nat. K. Slevogt + D-82362 Weilheim + Tel (0881) | €3 - 0 + Ttx 881 803 + Fax (0861) 6 25 39 BA OxiStirrer/RZ300/1/93/1; TM/Mai 53Assembly of the WTW-flow system with stirrer Calibrate sensor with connected Center sample bottle on Immerse sensor into the RZ 300. Oxi-Stirrer. bottle; adjust position with fixing ring FR 19. Lower edge of the RZ 300 Depress at the instrument => max. 2 em above bottle Measure ground,Assembly of the WTW-flow system with flow-through accessory Fix D 201 at the stand Fix connection tubes (use PVC Inlet drain should be as short (Angle approx. 45°). ‘or Tygon tubes). as possible (e.g. use suction unit SCH 201). Connect outlet pipe with a Insert calibrated sensor Screw cap sturdy pump. {OxiCal’) into D 201 up to (Seal D 204). Adjust flow-through volume stop. between 25 and 65 mifmin, Oe Depress at the instrument => —_In case of high fluctuations of, Measure the measuring signal ground Measuring signal will be stable D201 via the grounding after approx. 3 minutes. socket, 55Technical Data Stirring accessory RZ 300 Material Rotor PE Rotor housing POM Magnetic Oxi-Stirrer 300 Admissible temperature = 10°C to + 50°C in air Voltage supply av Connector socket 2pin Stirring power sw Stirring quantity 100 ml to 500 mi Stirring drive Coll drive Rotational speed 930 rpm permanent rotational speed Finish Outer surfaces: Stainless steel Drive and electronics cated in PUR Dimensions 120 mm x 120 mm x 35. mm Weight Lake Flow-through accessory D 201 Opsensors EO 90, EO 96, TriOxmatic® EO 200 and 201, TriOxmatic® 300 Dimensions Total height 114 mm Diameter 39 mm (without connections) Connections Tube connectors for tubes with 4 mm inner diameter Grounding socket for brush plug (@ 4 mm) Liquid volume 3,8 mi with O,-sensor Flow-through volume 25 milmin for 99 % signal 65 milmin for 101 % signal Max. temperature 50°C Material - PMMA (Plexiglass) Flow-through chamber Pressure for pressureless operation only Recommended accessory Suction unit model SCH 201 (consisting of a suction pipe V4A, PVC- tube and hose clip) 56