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Biochemistry Homework - Jorge Gutiérrez - KI
Biochemistry Homework - Jorge Gutiérrez - KI
Engineering
BIOCHEMISTRY
1st Sem 2020
PA - BIOQUÍMICA - 2020SEM01
Table 1. Inverse of concentration and speed of the reactions in the presence of inhibitor.
As shown in class, since the Km values for the reactions are the same but the Vmax values are
Noncompetitive inhibition
0.2
0.18
0.16 f(x) = 0.14 x + 0.04
0.14
0.12
0.1
1/V
0.08
f(x) = 0.06 x + 0.02
0.06
0.04
0.02
0
0 0.2 0.4 0.6 0.8 1 1.2
1/[S]
Inverso velocidad sin inhibidor Linear (Inverso velocidad sin inhibidor)
inverso de velocidad con inhibidor 2 Linear (inverso de velocidad con inhibidor 2)
Figure 1. Lineweaver-Burk graph of the reaction without inhibitor and in the presence of inhibitor 2.
1
Biomedical
Engineering
BIOCHEMISTRY
1st Sem 2020
Vmax
V max app = (1)
[I ]
1+
KI
Data (obtained from class):
V maxapp =25 , Vmax=50 , [ I ] =5 mM
Solving:
[ I ] ∙ V maxapp 5∙ 25
K I= = =5 m M
Vmax−V max app 50−25