1/12/2020 BAM Media M19: Bismuth Sulfite Agar (Wilson and Blair) | FDA BAM Media M19: Bismuth Sulfite Agar (Wilson and Blair) January 2001 Bacteriological Analytical Manual M19 Bismuth Sulfite Agar (Wilson and Blair) Polypeptone (or peptone) | 109 Beef extract 59 Dextrose 59 NagHPO« (anhydrous) 49 FeSO, (anhydrous) 039 Bismuth sulfite (indicator) | 8g Brilliant green 0.0259 Agar 209 Distilled water ‘iter Mix thoroughly and heat with agitation. Boil about 1 min to obtain uniform suspension. (Precipitate will not dissolve.) Cool to 45-50°C. Suspend precipitate by gentle agitation, and pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let plates dry about 2 h with lids partially removed; then close plates. Final pH, 7.7 + 0.2 DO NOT AUTOCLAVE. Prepare plates on day before streaking and store in dark. Selectivity decreases in 48h. CAUTION: DO NOT AUTOCLAVE PREPARATION Day before use. STORAGE In Dark Loss of Selectivity after 48 h Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. hitps:wwfda.govtfoodilaboratory-methods-foodibam-madia-m19-bismuth-sulfte-agar-vison-ane-blait n