Professional Documents
Culture Documents
References:
https://www.nap.edu/read/11311/chapter/8#171
1 2 3 4
Figure 1
MATERIALS
computer 600 mL beaker
Vernier computer interface enzyme suspension
Logger Pro four 18 X 150 mm test tubes
Vernier Gas Pressure Sensor ice
1-hole rubber stopper assembly pH buffers
10 mL graduated cylinder test tube rack
250 mL beaker of water thermometer
3% H2O2 three dropper pipettes
PROCEDURE
1. Obtain and wear goggles.
2. Connect the Gas Pressure Sensor to the computer interface. Prepare the computer for data
collection by opening the file “06B Enzyme (Pressure)” from the Biology with Vernier folder
of Logger Pro.
3. Connect the plastic tubing to the valve on the Gas Pressure Sensor.
Part I Testing the Effect of Enzyme Concentration
4. Place four test tubes in a rack and label them 1, 2, 3, and 4. Partially fill a beaker with tap
water for use in Step 5.
5. Add 3 mL of water and 3 mL of 3% H2O2 to each test tube.
6. Using a clean dropper pipette, add 1 drop of enzyme suspension to Test Tube 1. Note: Be
sure not to let the enzyme fall against the side of the test tube.
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Table 1
Test tube label Volume of 3% H2O2 (mL) Volume of water (mL)
1 3 3
2 3 3
3 3 3
4 3 3
7. Stopper the test tube and gently swirl to thoroughly mix the contents. The reaction should
begin. The next step should be completed as rapidly as possible.
8. Connect the free-end of the plastic tubing to the connector in the rubber
stopper as shown in Figure 3. Click to begin data collection.
Data collection will end after 3 minutes.
9. If the pressure exceeds 130 kPa, the pressure inside the tube will be too
great and the rubber stopper is likely to pop off. Click on Stop if you
reach 130 kPa or if the stopper pops off.
10. When data collection has finished, pull out the stopper and empty the
test tube. Figure 2
11. Find the rate of enzyme activity:
a. Move the mouse pointer to the point where the data values begin to increase. Hold down
the mouse button. Drag the mouse pointer to the point where the pressure values no longer
increase and release the mouse button.
b. Click the Linear Fit button, , to perform a linear regression. A floating box will appear
with the formula for a best-fit line.
c. Record the slope of the line, m, as the rate of enzyme activity in Table 4.
d. Label this line on your graph. To do this go to insert on the tool bar. In the pull down
menu pick “text annotation.” A text box will appear. Type in the box what this line
represents - in this case type “one drop.” You can click on the box and move it to a
convenient place.
e. Now go to experiment on the tool bar. To keep this line on your graph and prepare for the
next test, click on “store latest run.”
LABORATORY ORGANIZATION (8629)
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Chemistry Lab: States of Matter
Product Description
This lab is a student favorite - a great day to have fun and build student relationships while
reviewing states of matter and discussing a non-Newtonian substance. Included a warm-up/exit
ticket for watching quick You Tube video's on cornstarch and non-Newtonian substances.
PDF/Word
Students:
• Draw the particulate view of solids, liquids and gases
• Link the idea of molecular spacing to state of matter
• Read about LP and compressing a gas
• Review properties of solids, liquids and gases
Lab Contains:
• Student Lab Sheet
• Student Lab Make-up Sheet for Absent Students
• Student Lab Sheet with Answers in Italics
• Lab Quiz - Use as exit ticket, next day warm-up or quiz
• Lab Quiz Answer Key
• Teaching Tips and Set-up Directions
References:
https://www.teacherspayteachers.com/Browse/Search:fun%20chemistry%20lab
Science laboratories have been part of high school education for two centuries, yet a clear
articulation of their role in student learning of science remains elusive. This report evaluates the
evidence about the role of laboratories in helping students attain science learning goals and
discusses factors that currently limit science learning in high school laboratories. The committee
carried out its charge through an iterative process of gathering information, deliberating on it,
identifying gaps and questions, gathering further information to fill these gaps, and holding
further discussions. In the search for relevant information, the committee held three public fact-
finding meetings, reviewed published reports and unpublished research, searched the Internet,
and commissioned experts to prepare and present papers. At a fourth, private meeting, the
LABORATORY ORGANIZATION (8629)
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committee intensely analyzed and discussed its findings and conclusions over the course of three
days. Although the committee considered information from a variety of sources, its final report
gives most weight to research published in peer-reviewed journals and books.
At an early stage in its deliberations, the committee chose to focus primarily on “the role of
high school laboratories in promoting the teaching and learning of science for all students.” The
committee soon became frustrated by the limited research evidence on the role of laboratories in
learning. To address one of many problems in the research evidence—a lack of agreement about
what constitutes a laboratory and about the purposes of laboratory education—the committee
commissioned a paper to analyze the alternative definitions and goals of laboratories.
The committee developed a concept map outlining the main themes of the study and organized
the three fact-finding meetings to gather information on each of these themes. For example,
reflecting the committee’s focus on student learning (“how students learn science” on the
concept map), all three fact-finding meetings included researchers who had developed innovative
approaches to high school science laboratories. We also commissioned two experts to present
papers reviewing available research on the role of laboratories in students’ learning of science.
At the fact-finding meetings, some researchers presented evidence of student learning
following exposure to sequences of instruction that included laboratory experiences; others
provided data on how various technologies contribute to student learning in the laboratory.
Responding to the congressional mandate to meet the mathematics and science needs of students
at risk of not achieving state student academic achievement standards, the third fact-finding
meeting included researchers who have studied laboratory teaching and learning among diverse
students. The committee took several steps to ensure that the study reflected the current realities
of science laboratories in U.S high schools, addressing the themes of “how science teachers learn
and work” and “constraints and enablers of laboratory experiences” on the concept map. At the
first fact-finding meeting, representatives of associations of scientists and science teachers
described their efforts to help science teachers learn to lead effective laboratory activities. They
noted constraints on laboratory learning, including poorly designed, overcrowded laboratory
classrooms and inadequate preparation of science teachers. This first meeting also included a
presentation about laboratory scheduling, supplies, and equipment drawn from a national survey
of science teachers conducted in 2000. At the second fact-finding meeting, an architect spoke
about the design of laboratory facilities, and a sociologist described how the organization of
LABORATORY ORGANIZATION (8629)
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work and authority in schools may enable or constrain innovative approaches to laboratory
teaching. Two meetings included panel discussions about laboratory teaching among groups of
science teachers and school administrators. The history of laboratories in U.S. high schools has
been affected by changing views of the nature of science and by society’s changing goals for
science education. Between 1850 and the present, educators, scientists, and the public have, at
different times, placed more or less emphasis on three sometimes-competing goals for school
science education: (1) a theoretical emphasis, stressing the structure of scientific disciplines, the
benefits of basic scientific research, and the importance of preparing young people for higher
education in science; (2) an applied or practical emphasis, stressing high school students’ ability
to understand and apply the science and workings of everyday things; and (3) a liberal or
contextual emphasis, stressing the historical development and cultural implications of science
(Matthews, 1994). These changing goals have affected the nature and extent of laboratory
education.
References:
https://www.tandfonline.com/doi/abs/10.1080/0140528860080208?journalCode=tsed19