Title of The Experiment:

Isolation of Ovalbumin
(Pre-Laboratory Report)

Objectives of the experiment:

1. Isolate ovalbumin from an avian egg white

2. Properly prepared buffered saturated ammonium sulfate reagent

Methods:

Materials:

- Ovalbumin - protein
- Egg white
- Beaker
- Centrifuge
- Centrifuge tube
- Hot plate
- Dropper
- Cheesecloth top-load balance
- Stirring rod
- Graduated cylinder

Process:

Preparation of buffered saturated ammonium sulfate

- Addition of 35.0 g ammonium sulfate

- Heating with occasional stirring
- Addition of 35.0 g ammonium sulfate
- Cooling
- Addition of 45 ml acetic acid

Isolation procedure

- Addition of 0.5 ml acetic acid to collected egg white

- Filtration with cheese cloth
- Addition of equal volume of buffered ammonium sulfate
- Mixing for 15 minutes
- Centrifugation (6000 rpm, 4 degrees Celsius, 4 minutes)
- Final addition of ammonium sulfate salt and stirring
Hazards:

- Chemical reagents
- Ammonium sulfate
- Distilled water
- Sodium acetate
- Glacial acetic acid

MSDS ( Mmaterial Ssafety Data Sheet)

Hazards/chemicals Potential health effects First Aid

AMMONIUM * Contact can irritate horoughly after handling Do not
SULFATE- Ammonium the skin and eyes. * eat, drink or smoke when using
Breathing Ferrous this product Avoid breathing
sulfate is an
Ammonium Sulfate can dust/fume/gas/mist/vapours/spray
inorganic sulfate salt
irritate the nose and throat Use only outdoors or in a well-
obtained by reaction of
causing coughing and ventilated area Wear protective
sulfuric acid with two
wheezing. * High exposure gloves/protective clothing/eye
equivalents of ammonia.
may cause nausea, stomach protection/face protection
A high-melting
pain, diarrhea, vomiting and Specific treatment
(decomposes above
drowsiness. * Prolonged eye
280℃) white solid which
contact may cause a
is very soluble in water
brownish discoloration of the
(70.6 g/100 g water at
eyes.
0℃; 103.8 g/100 g water
at 100℃), it is widely
used as a fertilizer for
alkaline soils.
SODIUM ACETATE- Symptoms may include Inhalation: Remove to fresh air. If
is an coughing, sore throat and breathing is difficult, administer
organic sodium salt. It labored oxygen. If the victim is not
contains an acetate. breathing. Ingestion: In breathing, provide artificial
ChEBI. Sodium large doses may produce respiration. Get medical attention if
Acetate is chemically abdominal pain, nausea, and symptoms persist]
designated CH3COONa, vomiting. Skin Contact: It Ingestion: Do not induce vomiting
a hygroscopic powder may cause irritation with
except as directed by medical
very soluble in redness and pain. Eye
personnel. If swallowed, give several
water. Sodium Contact: Sodium acetate
anhydrous contact may glasses of water to drink. Never give
acetate could be used as
cause irritation, redness, and anything by mouth to an
additives in food,
pain. unconscious person. Get medical
industry, concrete
attention.
manufacture, heating
pads and in buffer Skin Contact: In case of contact,
solutions. flush skin with plenty of water.
Remove contaminated clothing and
shoes. Wash clothing before reuse.
Get medical attention if irritation

GLACIAL ACETIC
ACID-  is a name for
water-free
(anhydrous) acetic
acid. Similar to the
German name Eisessig
(ice vinegar), the name
comes from the ice-like
crystals that form slightly
below room temperature
at 16.6 °C (61.9 °F) (the
presence of 0.1% water
lowers its melting point
by 0.2 °C).
Acetic acid can be a
hazardous chemical if not
used in a safe and
appropriate manner. This
liquid is highly corrosive to
the skin and eyes and,
because of this, must be
handled with extreme care.
Acetic acid can also be
damaging to the internal
organs if ingested or in the
case of vapor inhalation.
persists.
Eye Contact: Check for and remove
contact lenses. Immediately flush
eyes with gentle but large stream of
water for at least 15 minutes, lifting
lower and upper eyelids
occasionally. Get medical attention
if irritation persists
Get medical aid immediately.
Skin: In case of contact,
immediately flush skin with plenty
of water for at least 15 minutes
while removing contaminated
clothing and shoes. ... If victim is
fully conscious, give a cupful of
water. Never give anything by
mouth to an unconscious
person.

Chemical reagents: are classified according to

their hazardous nature, such as being flammable, harmful, toxic, irritant,
corrosive, hazardous when decomposed during storage or dangerous for
the environment. Many reagents consist of a combination of
such hazards.