Practical Session Fifteen- Creatinine Clearance

Objectives of this practical:

 To learn the procedure for the collection of 24 hour urine sample

 To understand how to calculate clearance
 To understand how to calculate body surface area
 To understand the use of the Cockcroft and Gault equation for calculation of
creatinine clearance and to compare the result with the convention method of
calculation

Results
Table 1: Precipitation of protein serum

Test Tube Sample in Serum Diluted Creatinine Distilled Trichlo

test tube Urine Standard water
1. Blank 0.0 ml 0.0 ml 0.0 ml 1.5 ml 0.5 ml
2. Serum 0.5 ml 0.0 ml 0.0 ml 1.0 ml 0.5 ml
3. Urine 0.0 ml 0.5 ml 0.0 ml 1.0 ml 0.5 ml
4. Creatinine 0.0 ml 0.0 ml 0.5 ml 1.0 ml 0.5 ml
Standard

Table 2: Assay of Creatinine in all samples from test tubes in table 1:

Test Solution Supernatan Solution Supernatant Picric NaOH Abs

Tubes Tube 1 t in Tube 2 Tube 3 in Tube 4 Acid Solution 505nm
(Blank) (Serum) (diluted (Creatinine (ml) (ml)
urine) standard)
Blank 1.0 ml 0.0 ml 0.0 ml 0.0 ml 0.5 0.5 0
Serum 0.0 ml 1.0 ml 0.0 ml 0.0 ml 0.5 0.5 0.855
Urine 0.0 ml 0.0 ml 1.0 ml 0.0 ml 0.5 0.5 0.003
Creatinine 0.0 ml 0.0 ml 0.0 ml 1.0 ml 0.5 0.5 -0.007
Standard