Tutorial Chapter 1

1. Example 6.1 A strain of mold was grown in a batch culture on glucose and the following
data were obtained (Table 1)  
a) Calculate the maximum specific growth rate
b) Calculate the substrate yield

Time (h) Cell Concentration Glucose

(g/L) Concentration (g/L)
0 1.25 100
9 2.45 97
16 5.1 90.4
23 10.25 76.9
30 22 48.1
34 33 20.6
36 37.5 9.38
40 41 0.63

2. Problem 6.1 (page 200, Shuler and Kargi (2002). Bioprocess Engineering Basic
Concepts Second Edition).

A simple batch fermentation of an aerobic bacterium grown on methanol gave the results
are shown in the table. Calculate

(a) Maximum Growth Rate

(b) Yield on substrate
(c) Mass doubling time
(d) Saturation constant
(e) Specific growth rate at t=10h

Time (h) X (g/L) S (g/L)

0 0.2 9.23
2 0.211 9.21
4 0.305 9.07
8 0.98 8.03
10 1.77 6.8
12 3.2 4.6
14 5.6 0.92
16 6.15 0.077
18 6.2 0