Responsible Report 2019 - 2020 (Rotation Plan) Responsible Remarks Laboratory of Environment and Food Safety (LEFS)

Laboratory of Environment and Food Safety (LEFS)
Responsible report 2019 - 2020 (Rotation plan)
Responsible Remarks
1 KEO Pheakdey
2 SEALONG Sreyneath
3 RIN Pheaktra
4 SOVANN Chakriya
5 THET Sopheak
6 PROEUNG Seyha
7 SEM Nita
30, October 2019

Responsible for report meeting 2020 (Rotation plan)
Responsible Remarks
1 RIN Pheaktra
2 SOVANN Chakriya
3 THET Sopheak
4 PROEUNG Seyha
5 YITH Vuthy
6 SEM Nita
7 KEO Pheakdey
8 SEALONG Sreyneath
6-Feb-20
LEFS Staff meeting report 2020
Date : 09/01/2020
Time : 10:30 AM
Lead by : SRENG Navin
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, Sem Nita, Proeung Seyha
Staff absent : KEO Pheakdey
Report Written by : Mrs. Rin Pheaktra
Topic discussed Action Responsible Date limit Remarks

Person
1. Big red Cap for Ice (not yet arrive so use our big cap sterile bottle as usual)
Receptionist
20mg/l of Na-thiosulfate For 1liter of Sample
New Bottle of 2. Small red Cap for treated Water (Tap Water, Drinking, Filtered Water, Bottled Water,
Need to be more careful
City Water ) Rin Pheaktra 13/01/2020 and explain the
Water sampling 20mg/l of Na-thiosulfate For 1liter of Sample
1
3. Small red Cap with green Label for Swimming Pool. customer to file the
120mg/l of Na-thiosulfate For 1liter of Sample sample 1L
For Micro Testing we use bottle which contain Na-Thiosulfate and for Chemical For Chemical testing
Start use on 13/01/2020 we use big red cap bottle without Na-Thiosulfate. should the same day
For Untreat water sample, we use sterile bottle as usual as reception
We create one form for one equipement and stick it near by the equipement,
Cleaninng Plan so that we can see the period of cleaing and also LEFS staff
2
sign on the paper when you have done cleaning
You can give any comment or suggestions. We have one week to review before we put
For any update for application.
3 We also have the acknowledge form for us to sign after our review. Anyone need to 9/1/2020
document review it.
Receptionist
4
4 If the number if douplicate with LBM we do not need to use it, we leave that number
Codatech and take note in the table
Rin Pheaktra 9/1/2020
5 Maldi-TOF Sub Culture Cronobacter for testing Maldi-TOF Machine Sovann Chakriya15/01/2020
Laborator
LEFS
Date : 17/01/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, SEM N
Staff absent :
Report Written by : SOVANN Chakriya
Topic discussed
1
Lab accreditation
External Audit
2
3
Quality Meeting
4 Audit with UN
7
7
10
11
12
13
14
15
16
17
Laboratory of Environment and Food Sa
NG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, PROEUNG Seyha, KEO Pheakdey
by : SOVANN Chakriya
Action
1. What is lab accreditation? It's a standard pattern that we request as a refference parameter according to criteria and auditio
2. what's the different between accreditation and cerification? Accreditation is refered to quality managerment and technical
audition. Certification is reffered to only document of results and they are not ensured about technical.
3. ISO 17025 set up from janaury 2020 until December 2022 with Water and Food Microbiology (must done).
1. 1st audit they will ckeck all parameters that we request. 2nd audit (every 18 months) they will pick some of parameters to
check both documents and technical until 5 years we will have main audit as a global to check that we've done for their criteri
not.
2. We need 6M to get acceditation such as method, management, staff, materials, consumable, and locaux.
B Pheak will have a meeting with all technicians that related to quality managerment.
we will have an audit with UNIDO on 27 January 2020 start from 9am until 5pm.
y (LEFS)
Responsible Person Date limit Remarks

Date : 30/01/2020
Time : 10:30 AM
Lead by : THET Sopheak
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, Sem Nita, Proeung Seyha
Staff absent :
Report Written by : THET Sopheak
Responsible
Topic discussed Action Date limit Remarks
Person
Share responsible for SOP Sopheak send template sumit 1 SOP/PROCEDURE
1 All staff
/PROCEDURE SOP/PROCEDURE on share file within two week
on 3rd week after summit
2 Presentation of SOP/PROCEDURE will select follow up
SOP/PROCEDURE
every 2 week but mayb impossible

to follow
up discuss each learn
3 Rotate plan basic of each
Chakriya - reception
activities
Seyha - water micro
Nita - food micro
4 List parameters chemical with to know the priority of the next limit deadline
critical point parameters Vuthy no limit deadline
List parameters and their effect Vuthy - chemical Vuthy
5 next limit deadline
when reach over limit Navin - Micro Navin no limit deadline
1-Physical inventory: Already done
2- Security audit
3- Evacuation plan-All drawings are
done!
4- COD in wastewater
5- Fire safety trainees
6- List of responsible person CCQHS
7- Kaptitude training
8-training
-Oceasoft training: done 06/12/2019 at
Epi/ Vir Room at 14h00.
-Discussion Metrology and LBM: Done
09/01/2020 at Epi/ Vir Room 14h00.
-Critical Equipment: Pipettes, Balances,
Masses, Climatique Chambers, Next CCQHS meeting 25/03/2020
Sopheak
6 CCQHS meeting Centrifuges… Centrifuges…(07/02/2020 Critical Equipment :07/02/2020 at
at 14h30 to 15h30) Vuthy 14h30 to 15h30
9- Waste management
-Laboratory: Wastes are not disposed
according to its sorts
(e.g. Blood contaminated in normal
Some yellow bins are not autoclaved
before usage
Offices: No foods in offices. All collation
must be eaten at canteen only
10- Monthly improvement team
meeting: Meeting will be done
monthly-Discuss and solve
problems/complaints
11- Biobank software
12-Problem and suggestion
7 Bon travaux stmacher Vuthy 31/01/2020

8 Double verification result Vuthy 31/01/2020
9 Suggestion book all staff DONE in the office
10 Visitor log book Sopheak DONE on the desk infront

all visitor inside the lab must sign in the of reception
11 Management meeting every wek on Thursday
prevent confidential information of
customer from falling to the Sopheak
12 Confidentaility at reception room wrong hand (ex. List of customer, Pheaktra
result, information sheet..)
-other customer
-other staff
-cleaning staff
Date : 05/02/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SOVANN Chakriya, Sem Nita, Proeung Seyha
Staff absent : Sealong Sreyneath
Report Written by : Proeung Seyha
Responsible Date
Topic discussed Action Remarks
Person limit
Do not allow intern sudents do sample analysis that effected

1 Manage Room to results and for new staff at least 3 month within reading All staff 5/2/2020
SOP before start to do sample analysis.
Maximum 3 persons in a room

2 Limitation Room All staff 5/2/2020
Request for new staff if have any concerned with tasks.
3 New SOP We have new SOP thermotolerant coliforms All staff follow up
Equipment cleaning
4 All staff 5/2/2020
form
Due to having many different form and can confusing user,
we decide to use the old form and put in the lab corridor
Date : 13/02/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, KEO Pheakdey, SOVANN Chakriya, SEALONG Sreyneath, Sem Nita, Proeung Seyha
Staff absent : No
Report Written by : Yith Vuthy
Responsible Date
Topic discussed Action
Person limit
Verification of water bath and autoclave: lab should give number,
temperature & EMT to metrology lab Sopheak follow up
Information of Quality Metrology will calibrate thermometer at IPC, may be in 2021 CCQHS follow up
meeting
1 Calibration of sensor oceasoft: lab should provide number of critical sensor
(metrology requirement) on Sopheak follow up
07/02/20 to metrology in order to prepare back up
Training oceasoft: LEFS will propose at least 2 technicians to participate follow up
1-Pre-anlytique in reception room

2-reagent
2 Critical refrigerators in LEFS Sopheak follow up
3-culture media
New refrigerator are in oder to replace the old
Setting temperature on Technician need to follow up temperature of incubator on display with

3 All staff follow up
each incubator setting temperature (stick on each incubator)
After verification, this method will apply to surface and waste water All staff follow up
4 New E.coli method Waiting for validation new price from DAF follow up
Verification of method is not pass yet, will repeat Chakriya

17/02/2020
next week Vuthy
SOP of Salmonella & Legionella will release sooon, Chakrya will presence
5 New SOP All staff follow up
these SOP
Will have new form soon but now we continue to use
the old form
The person find problem/issue, go to see Sopheak to get the form, will fill
Non conformity
6 first part, then give to Sopheak to put a code, after that the responsible will All staff follow up
report
verify problem and find corrective action ASAP.
The responsible will find the root cause and discuss this non conformity in
the meeting to find out the preventive action.
Will print in color what kind of bottle can't use in chemical analysis and stick Sopheak 21/02/2020
Avoid confusing bottle in chemical lab & reception room
7
when analysis Will print in color what kind of bottle can't use in water micro analysis and
Sopheak 21/02/2020
stick in water micro lab & reception room
MOH information need to fill Pheaktra follow up
8 Information sheet
new information sheet May include criteria of customer, then we can give Navin Soon
conclusion on their sample
9 Chemical analysis List of chemical parameter with conservation condition and duration will Navin Done
release soon and stick in chemical lab and reception room
10 Planning of cleaning will valide and stick inside lab near water micro lab Sreyneath
equipment
21/02/2020
Vuthy will responsible for culture media production and control and Vuthy
chemical 13/02/2020
11 New responsibility
Navin
Navin and Sopheak will responsible for micro. 1 week each Sopheak 13/02/2020
Include Nita and Seyha in planning guard on Saturday 2 times/week starting from March
in March with other 2 staff
Sopheak
12 Planning Nita and Seyha may include in planning guard on Sunday and public holiday from April
from April 2020
All staff stay at the same post for march March
New organisation
13 after New recruit will be trained in sample reception, Nita will analyse food All staff 9/3/2020
Pheakdey leave micro., other will work in the same poste as previously
To avoid change or edit on the upper part of result from

14 Result form Sopheak follow up
Sopheak check the format
mention in the revised result that it replace old result Pheaktra
15 Revised result Done
and change date of new revised result NaVIN
1-follow bluesystem when do internal command from Chakriya Sopheak 13/2/2020
2-Chakriya should provide IPC code for each products Chakriya 13/2/2020
16 Stock management
3-after receiving material arrival document, Navin will send mail to inform
Navin 13/2/2020
for any material/reagent that is out of stock
eung Seyha
Remarks
2 Technicians + Nita/Seyha
After validation of his competent

Date : 21/02/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, PROEUNG Seyha, KEO Pheakdey
Staff absent : -
Report Written by : Ms. KEO Pheakdey
Responsible
Person
1. All excell data not follow the model
B. Pheak collect new model of excell data
from water/food micro
and chemical and them put together in a
folder
2. Collect all the model from all technician
1
All technician need to use this model for
Excell data All technicians excel
3. Verify all those models data 2020
4. Make a new one model of excell date before Avoid any modification for these new
we entire new data of 2020 models
1. Delete all code chemical analyse from food list Pheakdey
exept formalin
2
Codatech 2. Add a new code for testing E Coli by microplate Aya For Excell data
3. Add a new code for testing CF in food list Pheakdey
3 Type of sample Classify the type of sample

(Food: ready to eat, supplement…and water: RO, filtered...)
B. Vin For Excell data
4
Form of parasite Make a sheet for enter data of parasite Nita
1. Cotrol quilty of bottle Nita Nita: control the quality of bottle
2. The bottle with small red cap & no label not allow Receptionist: Manage all the bottle after
Bottle to enter the reception room
All technicians
cleaning
5
3. The person who bring the bottle after cleaning
to the reception room not allow to organize these All technicians
bottles into reception box
1. Carefully with the colonie growth on XLD because we have

detected Salmonella on XLD from water sample All technicians
with Rapid (-)
Culture medium 2. Count all colonies caracteristic of salmonella on XLD or R'sal All technicians
6 because it is very important for confirmation test
(XLD and Skimilk)
3. Note CC number of Salmonella and select 1 to confirm,
if negative pick other 4 to confirm in the same time
3. Make a sheet for skimilk production and quality B.vuthy
control of skimilk
Frequency : Control every 2 week

Room: Water, Food, culture media,
1. Read the new a guideline about environment control
Incubation, pathogen and inside biosafety
cabinet
Environment During analysis :

7 -culture media: when pouring culture
Control
2. Set the date to do the controll All technicians media
-Water :when inoculation for FTM
-Food: when prepare decimal dilution
3. The room responsible have to do the envionment Using worksheet of food microbiology
control by themselves (feuille paillasse)
All staff have to submit the monthly report for the last month before 30th
8 Monthly plan and have to send a new plan for new month
All staff
of each month
9 Reference strain Sub culture the refernce strain form -80 ⁰C

Neath
Show the difference point from the old SOP
Presentation of
10 salmonella
and Legionella Question and answer about new SOP Chakrya
Presentaion of
11 sero group of Next week B. vuthy Next meeting
salmonella
1. All the activities that we have done should have a
12 Others traceability because now we are processing to be a lab
standard
No traceablity when control
We don’t know when the control was conducted and by who From now nita responsible
13
Control expired and what was the result. And we need all those information Nita
use Worksheet to record the control
water bottle to show what we have done. and submit to sopheak to keepfor
traceabiliy
14 Command extern Follow up bong thea Bong thy

Out of stock
Non conformity Sopheak
15 RVs in wrong No Non conformity report
to check
incubator
16 PH meter HANA Control PH not working Bong thy

Use Conductivity equipment for pH for WATER analysis and Neath
Laboratory of
Date : 27/02/2020
Time : 10:30 AM
Lead by : Sopheak Thet
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, PROEUNG Seyha, KEO Pheakdey
Staff absent :
Report Written by : SEM Nita
Topic discussed
1
Quality
2 Excel Form Update
3 RAEMA Control
4 Internship Student
4 Internship Student
5 Water Analysis Result Record Update
Presentation
N Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, PROEUNG Seyha, KEO Pheakdey
Activities
Don't open Incubactor (41.5C) too long since it used to error because we used it dry media and open it over time.
Problem aircon, how many month they need to clean ?
Prepare order Anios spray full in stock
Stock controler need to manage stock anios and mask carefuly
All responsible of food micro, water micro and chemical analyse have to make their own model of excel and then send it to B. Pheak
B. Pheak have to make a new folder to put these model
Reama will come to lab on 11/03/2/2020

Reama control test start on 16/03/2020 About two weeks will be complate.
5 Staff has to join such as B.Pheaktra, B.Pheakdey,SreyNeath,Chakriya,Nita
Master 1: come to lab on 29/03/2020 She had 2 months stay in the lab and Related Documents and SOP with B.Pheak
Master 2: come to lab on 09/03/2020 She had 6 months stay in the lab and Related Markering Social
Spore : Ex CC 2 => N=2x2=4, Spore Day1 CC= 2 Day2 = 0* => N=2x2=4CFV/1ml
Entero : Ex CC =0* => N>150, CC=2 and N=2
Psedo : CC=0* => N= Presence or CC=4 =>N=4
FTM : 1ml =0* and 10(-1)=0* >300 =>N >3,10(3) CFV/1ml if 10(-2) >300 =>N>3.0x10(4)CFV/1ml
Nita present Listeria monocytogenes
Seyha present E.coli
Responsible Person Date limit Remarks
B.Pheak
LESF Team
B.Pheak
Chakriya
Laboratory o
LEFS
Date : 06/03/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, S
Staff absent :
Report Written by :
Topic discussed+A12:G26A12:G30
Non-comformity
2 Wasting Media
3 Reception
4
Salmonella Spp
5 Prevent Insect
6 Lab Security
6 Lab Security
7 Environment control
8 Gas supply security
9
English /French Course request
(formation continue)
11 Permenance non-comformity
12 New staff
Phum Ben & Khmer

13
New Year
14
Culture Media Inventory
Laboratory of Environment and Food Safety (
n, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, KEO Pheakdey
PROEUNG Seyha
SEA Longsreyneath
Action
Problems:
TS broth run out off stock and food analysis has met TS crisis.
Cleaned Tube has not enough for using in production, since tube cleaner return
tube late since he wash not on time. By the ways, media producer didn't follow up
with him reqularly.
Solutions:
Mr.Seyha need to write Non-comformity sheet after he come back on Tuesday.
Brother Vuthy need to reminds Seyha to ask Mr.thea about tube cleaning reqularly.
Note that a week in case we have transferred twice times so we need to take one time cleaned tube from him back. Moreove
cleaned tube and tube cleaner, he cannot transfer tube to us on time, media producer or people who related need to release
Seyha need to add TS, ED, Steril Tube, NaCl to stock inventory.
Seyha need to do inventory on total Tube and Flask in using stock.
To prevent wasting Media so media producer need to prepare EPT broth (750-800 ml) in 1L flask for using with 3 food sample
Since we have lack quantity of flask 1L ,Seyha need to use flask 500ml some for Yeast Extract
and some for TCS.
Reception, she has no concentration on her work.
So all LEFS staff are not allowed to come and talk about useless things with her while she is typing
results or register samples
From now when we find Characteristic Colony need to pick up 5 to isolate on TCS. Then take only
1 plate (C1) to do oxydase--> Api-20E and other 4 plates need to keep in fridge during waiting C1
identify.
In case we can not catch Salmonella in C1 then we need to take those 4 CC plates to re-isolated and do
oxydase--> Api-20E at the same time.
water and food analysis need to clear waste plates reqularly after result release.
Ms. Charkya need to order insect spray for using in that room.
Related to last week mr.Chea (Virology staff) entry our lab without permission and lab uniform.
To prevent this security problem Ms.Sopheak need to print one more No entry without duty in Khmer version and stick it in la
and stick on lab door.
Problems:
Since on february control, the result was not accurated and acceptable.Espacially, culture media room.
Solutions:
Food&water technician need to manage time take media prevent entry media room during media pouring.
Controllers need to control air flow in their rooms during do control. And prevent people
qauntity during control time.
Seyha need to prepare space for storage VF and Yeast Exact at oven side for water analyzer .
Gas supply flow in lab is not work sometimes.
Ms. Sopheak need to ask security team to fix this problem.
LEFS staff can request Enlish/ French learning with reason that support request to Head LEFS.
Problems:
Ms. Sreyneath forget her permance duty on 01/03/2020.
Water analysis parts missed and delay time of identify. And Some water samples has requested for Coliform and E-coli.
Solutions:
Repeated analysis on sample that has problem with Coliform and E-coli.
to prevent this case happen again, the person who responsible on duty need to take note about date of their
permenance on Callendar or their cell phone. And partner who responsible on same day should remind his/her partner via sm
Water & Food analyzer need to defined the optimal time for fix time permenance duty next.
She will arrive lab on 12/03/2020.
Ms. Sopheak need to add her to next Saturday plan shcedule.
Next, Lab cannot close both Phum Ben & Khmer New Year so LEFS need to choose one occasion to close lab.
LEFS agree and choose to close lab on Khmer New Year.
Seyha need to do inventory in all Culture medias next week.
And then Seyha need to do stock inventory 1 times per 2 weeks.
Responsible
Date limit Remarks
Person
Bro.Vuthy
&
Seyha
12/03/2020
12/03/2020
Seyha 06/03/2020
LEFS 06/03/2020
LEFS 06/03/2020
Ms.Pheakdey
& Chrakya 6/3/2020
Ms.Charkya
Ms.Sopheak 12/3/2020
12/3/2020
Ms.Pheakdey
Ms.Charkya
6/3/2020
Mr.Nita
Mr.Seyha
Ms. Sopheak Next CCQHS
LEFS 15/03/2020
LEFS
6/3/2020
12/3/2020
Ms. Sopheak
LEFS
2020
Mr.Seyha 12/3/2020
Date : 12/03/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, KEO Pheakdey,
PROEUNG Seyha, KEO Sophaneth, Ludwine LAURETTE
Staff absent : RIN Pheaktra, KEO Pheakdey (Busy with registration samples)
Report Written by : SOVANN Chakriya
Responsible
Person
1. Ms. Ludwine LAURETTE : French student
1 Introduce New Comer 2. Ms. Sophaneth KEO : new staff
1. Prepare EPT broth (750-800 ml) in 1L flask for using with 3 food samples and Seyha Continue Bro.Vuthy
Compled
prepare it. & Seyha
2. Since on february control, the result was not accurated and acceptable.Espacially,
culture media room,
Follow up old report So Seyha need to prepare space for storage VF and Yeast Exact at oven side for Compled
2 Seyha
meeting water analyzer that not effect
to his activity during they take the media.
3. Inventory in all Culture media, TS broth, ED sterile, NaCL solution, and Flacon. Seyha 16.03.2020
then Seyha need to check stock inventory every 2 weeks.
Reminder
Reception, she has no concentration on her work.
3 Reception So all LEFS staff are not allowed to come and talk about useless things with her LEFS
while she is typing
results or register samples
Submit before 4pm on 12.03.20
4 English Course request LEFS
After dicussion about duration of incubation time on each parameters in food and water,
we will fix time to
work on saturday and Sunday from 1pm to 4pm.
5 Fix permanence time
Aya
Then Aya and Pheakdey will make the timeline on what bacteria that we should confirm first.
+ Pheakey
1. Highlight yellow on which parameter that not satisfactory

2. Enter only MOH on client instead of company's products. We just want to know the number of
6 Excel Data in food products LEFS
that MOH bring it to test.
1. For all type of water except bottle water, we will use 2 tubes of 10 ml to test.
Aya 16.03.2020
2. For bottle water, we will test 2 tubes of 10 ml too but convert result to CFU/50ml.
7 Spore 3. Bro Vuthy have to find new tube for testing spore 20 ml. Bro.Vuthy
1. Bong Vin will have a meeting with Director about Covid 19, so we can ask any question
and also survey us
or tell what we are concern to Bong Vin.
8 Covid 19 2. As you know Covid 19 was tested in IPC and we're also working here, so we should LEFS
not reply or comment
something that related to Covid 19 on social media. It's not allowed.
1. 5 technicians : Pheaktra, Pheakdey, Sreyneath, Chakriya, and Nita.

2. 5 powder samples noted as number 1White, 2Red, 3Yellow, 4Blue, and number 5Green.
3. 1 technician test 2 samples : Ex 1st sample weight 25g for sal and 25g for listeria
detection, 2nd sample
weight 10g for other parameters and 10g for listeria enumeration.
4. Leave sample 30mn before weight and keep it 20mn after weight + EPT, then start
make dilution.
5. Enterobacteria : incubate at 30C
6. Parameter:
FTM -4
Enterobacteria such as CT, CF, E.coli, EB dilution to 10-3 But for 10-1 make 1ml 3 plates
and pour media
immediately for 10-1
9 REAMA Control LEFS
Cp dilution to 10-2 But for 10-1 make 1ml 3 plates
9 REAMA Control LEFS
Staph <100, so dilution to 10-2 and spread 0.1 ml

Listeria enumeration <10, so dilution to 10-2 But for 10-1 spread 1ml 3 plates,
10-2 Spread 0.1ml
Listeria detection : process Enumeration first and then keep it for detection
Salmonella: after process other parameters
6. Confirm Listeria: Pick up 5cc isolate on Blood agar (3colonies per plate) + read Hemolysis
Gram
Catalase
Mobility use BHI
Date : 19/03/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, Rin Pheaktra, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, KEO
Pheakdey, PROEUNG Seyha and KEO Sophaneth
Staff absent :
Repo: Rin Pheaktra
Responsible Date
Topic discussed Action Person limit Remarks
*If someone feel sick or abnornal, please inform to our teamwork about your
situation,
19/03/2020
Do not come to work, Quarantine yourself at home, If still not get better, Please
call to 115
1 Covid-19 All IPC staff

*HR will give us the permission according to the real situation (Reasonable) 19/03/2020
*At the entry of IPC, the security will check the Temperature of each staff 22/03/2020
*Only IPC Staff and customer allow to inter IPC Campus (No entry without
permissioin) 22/03/2020
2 Confused Covid sample Virology team will response Virology staff Still happen on 24
to our reception March 2020
Mr. Kean prepare and provide for all lab, Use Alcool for desinfect the surface of
3 Alcool and Gel workplace All staff 23/03/2020
and Alcool Gel for hand sanitizer
4 SOP Blank Test Pheakdey to the presentation of Blank test SOP All staff
Date : 27/03/2020
Time : 9:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita, KEO Pheakdey,
PROEUNG Seyha, KEO Sophaneth
Staff absent :
Responsible
Person
1. If situation evolution : reduce mode

Team 1: B. vin, Sopheak, Sreyneath, Nita, Sophaneth
Team 2: B. Vuthy, Pheaktra, Chakriya, Seyha
2. Reserve LEFS for Viro: PCR (B.Navin, B.Vuthy, Sopheak) and data entry (all LEFS Staffs)
3. Mask now priority is Viro unit
so try to reduce using mask if not necessary : All LEFS
1 Covid Culture media: need mask STAFF
Food and water analysis: no need
Reception : Need mask
Parasite: no need
4. Glove: no glove in reception
5. Maintain quality and respect good laboratory practice even in reduce mode
Sopheak
2 Limit activities in IPC Inform all LEFS customers : reception only in morning, work from morning and afternoon
& Pheatra
26.03.2020 Done
3
Traceability of environment put Environment control sheet first in each folder of food microbiology (before food traceability) All LEFS 27.03.2020
control Staff gard on Sat and Sunday must follow up STAFF
4
Tracebility of removal all removal culture media (bad qaulity, damage...) must note in this form every week per month Seyha 27.03.2020
Culture media Form review
5
Timeframe for permanance 12:30 pm to 3:30 pm
Chakriya combines the time of food and water analaysis
All LEFS
1/4/2020
Sat and Sunday Applied from April STAFF
Sunday: Pheaktra, Neath and Chakriya

Saturaday : exclude Bong vin from April, Nita and Seyha come together, Sphaneth comes every
week
Pheaktra : Culture media All LEFS
6 Working plan Neath: Chemical water + QC media STAFF
1/4/2020
Nita: Food MICRO
Seyha: Water micro
Charkriya : Responsible for microbiology of food and water
1. SOP Non confomity of Sample Pheaktra
SOP/ PROCEDURE
2. Translate interpretation result of Microbiological parameter
7 Sopheak
3. Rearrange food Microbiology criteria
8
Presentation 1. SOP sample reception process : Sophaneth
2. Interpretation result of Microbiological parameter : Bong Vin
Date : 01/04/2020
Time : 9:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, SEALONG Sreyneath, SEM Nita, KEO Pheakdey,
PROEUNG Seyha, KEO Sophaneth
Staff absent : SOVANN Chakriya
Responsible
Person
1. reduce mode from 6 April 2020

All LEFS staff
2. Welcome customer only the morning, but staff work full day
1
Limit activities in IPC 3. Sampling can do both morning or afteroon Nita 06/04/2020
concerning COVID
Sopheak
4. inform all LEFS customers contract and non contract Done
& Sophaneth
1- April 8, last day of salmonella

Limit activities 2- April 7: last day for Y/M and Listeria Phaneth need to inform customer when they come
All LEFS
2 3- April 2: Legionella to pick up bottles
Khmer new year 4- April 8: water standard
staff
Navin inform Nagaworld (done)
5- Close and no guard during KNY
3
Follow up food all sample that the result already out, Food responsible person
Nita 1/4/2020
sample disopsal must note before throw them away
Note open date or
after opened commercial reagent or prepare any reagent, staff All LEFS
4 preparation date for must note the date of opened or the date of preparation staff 1/4/2020
all using reagents
pheaktra day off from 5 april until after KNY, so must prepare
5 Culture media enough culture media in stock
Pheaktra
6 Sample PFR 06 April 2020
7 COFFEE CONCEPTS 7-8 April 2020

Date : 21/04/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SEM Nita, SOVANN C
Staff absent : KEO Sophaneth
Report Written by : PROEUNG Seyha
Topic discussed Action Responsible

Person
1. Seyha resond water Microbiology and Nita Food microbiology A Ya, Nita,
2. A Ya will cover Seyha and Nita Seyha
3. If B Pheaktra still continue work, She will respond with Water

1 Planing for May B Pheaktra
Chemistry and B Neath will rotate to Media Production. And in
and B Neath
case she leaves work still keep the same post.
In case LEFS will not recruit for a new staff when B Tra leaves
work, and all LEFS staffs have to help produce Media as All LEFS staff
immediately
2 Receptionist From 27/04/2020 LEFS will open full day as normal Sophaneth
1. SSPI : Screening 100 patients with 2500 samples from Calmet

hospital to detect ESBL pathogenic.
3 Projects
2. AMR : will run mid of next year.
3. CAPFISH : To define contanminated fish and fish products from

Seim Reap, Bathambong and Kampot.
4
Reading result & Inform to B Vin, incase B Vin absence or busy must to replace B
interpretation Pheak when reading result
Food Safety (LEFS)
rt 2020
ath, SEM Nita, SOVANN Chakriya, PROEUNG Seyha
Date limit Remarks
Done Sophaneth will drop email inform to customer
2 years
3 months
Laboratory of Environment and Food Safety (LEFS
Date : 07/05/2020
Time : 10:30 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, RIN Pheaktra, SEALONG Sreyneath, SEM Nita, PROEUNG
Staff absent : SOVANN Chakriya, KEO Sophaneth
Report Written by : Yith Vuthy

Person
Open BSC before work
Calculate media before 11h30, so could dissolve them at 1h30
Alarm of high temperature during testing (>27ºC), working
temperature must be 18-27ºC. Check/see temperature display on
OCEASOFT.
Take all media need one time a day, do not go in and out
frequently from media room
1 General Remark Level wate rin water bath must be over level media All LEFS staff
Cover of plate (BP, Mossel) must not under hand during spread
plate
All sample must be homogenize by stomacher (max 2min), except
liquide and powder
For incubation, only 6 plates/range; if no space, it's ok if over 6
plates/range
Nita and Seiha should participate in reading plate and confirm
When reading plate: TBX and VRBL (C>300CFU), but FTM: 0;

2
Check contamination of So nita will test on TBX and VRBL after sample analysis: 1ml of Nita
sample water bath, bottle falling in water but tightly close, and bottle
falling in water but not close well
Nita will work in water chemistry and control media

Neath will work in culture media production
3 Planning for May 2020 Seyha will work in water micro. All LEFS staff
Chakriya will work in food micro.
Phaneth will work in reception
Planning Writing Share planning for writing SOP/Procedure and submit for every 2
4 All LEFS staff
SOP/Procedure week
Food Safety (LEFS)
ort 2020
SEM Nita, PROEUNG Seyha, KEO Sophaneth
Date limit Remarks
Immediate
08/05/20
12/5/2020
Date : 13/05/2020
Time : 10:30 AM
Staff join: SRENG Navin, THET Sopheak, RIN Pheaktra, KEO Pheakdey, SEA Longsreyneath, Sem Nita, Proeung Sey
Staff absent : SOVANN Chakriya
Report Written by : SEM Nita
Topic discussed
1
Media Production
2 Non Conformity
3 Cleaning waterbath
4 Equipment
Laboratory of Enviro
KEO Pheakdey, SEA Longsreyneath, Sem Nita, Proeung Seyha
Activities
After autoclave media producer need to check media's cap and make sure that the cap closed tightly.
Moreover, wash hands with alcohol before close media's cap.
Technicain who respond on food and water need to keep media stable in waterbath
Nita writing non conformity Media EPT contaminate.
Waterbath need to risen with soap and then clean with ED 2 times.
Technicain s need to clean waterbath Twice a week .
New Incubators will arrive soon and after that incubator 41.5⁰C which use for incubated Rvs will be stop using.
Responsible Person Date limit
Neath
Charkaya , Seyha
Nita
Technicain who respond on food and water

Remarks
Date : 04/06/2020
Time : 10:00 AM
Staff join: SRENG Navin, YITH Vuthy, THET Sopheak, SEALONG Sreyneath, SOVANN Chakriya, SEM Nita,
PROEUNG Seyha, KEO Sophaneth and HAM Menghor
Staff absent : No
Report Written by : KEO Sophaneth
Responsible
Person
Problem : Oxidase Test is not accurate.

Solution
1. Need to control positive for oxidase test of Pseudomonas aeruginosa.
All LEFS
1 Oxidase Test 2. Colonies for oxidase test must be fresh and completely isolated on TCS.
STAFFS
3. Dry Slide must be kept in an empty petri dish and cover with parafilm after using
(No more than a week)
(Note: Pseudomonas subculture is responsible by Water Analyzer)
1. There are 3 positions for female staffs to rotate such as Reception, Food and Water Micro.
2. There are 5 positions for male staffs to rotate such as Media Production, Food and Water Micro, Chemical
and Sampling.
- The aim of this rotation is too let all staffs understand and remember each position's tasks and All LEFS
2 Rotation Position responsibilities. So that we all can come on Sunday rotatably. STAFFS
- every 3 month, back to same post
Bong Neath will work as receptionist.
3
Planning for June and July Bong Ya, Bong Seyha and I will work as Food and Water technicians. All LEFS
2020 Bong Nita will work as Chemical technician and Media Controller. STAFFS
Menghor will work as Media Producer.
4
Receptionist for June and For these two months, reception post will be responsible by Bong Neath because it's provide more time for
Bong Ya to learn about receptionist's tasks. Moreover, Bong Seyha and I will have much time to learn about Bong Neath
July Water and Food Analysing from Bong Ya.
Samples need to be stored separately depend on sample types.

5
Sample Storage After - If samples which are stable at room temperature, they must be kept at room temperature. Food Techician
Analyzing - If sample which are spoiled at room temperature, they must be stored at -20 or 4 degree celcuis.
(Note: Bong Vin will inform later about UHT milk storage)
6 Food Sampling Food sampling will be responsible by Bong Seyha soon after Bong Seyha got driving licence. Bong Seyha
All LEFS
7 Training Test This training aims to strengthen staffs' skill particularly the rare experiement test.
STAFFS
For preparation of dilution, we can base on the previous result and criteria of each sample
8
Media Using Limitation For example , yeast and mould, if the criteria is 10000, we can do only 1000, we can interpret the result Food and Water
- We have to reduce using quantity of culture media. Example: Yeast and Mould analysis no ned to dilute Technicians
until 104 .
- Before
Date and place of sampling : 03/05/2020 at (20:08 pm) Naga Rock
Result Form Revising Date of reception : 04/05/2020 at (10:35 am)
9 - After Receptionist
Date and place of sampling : 03/05/2020, 20:08 pm at Naga Rock
Date of reception : 04/05/2020, 10:35 am
Sample Registration of
- In case customers request Micro Plate for drilling water, receptionist have to register every single
10 Micro Plate (Drilling Water) Receptionist
parameter without package price.
Cronobacter spp Testing for

- All samples from MOH costs only 60$ except Cronobacter Spp parameter which is needed to charge more
11 MOH for 60$.
Receptionist
12 Food testing liquid-criteria <1, use 1 ml of sample and serial dilution Food analysist
semi-liquid-criteria < 10 , weight 10g and serial dilution
13 Key collect all room keys and keep the box in Bong vin Room Sopheak
14
Customer Quotation and Delay of response=24 hours Bong thy and
Complaint however if cant response, must acknowledge receipt first receptionist
now we have a list of food micro criteria and all staff especially receptionist can review:
-Red ighlight=Stap<10 need to becareful All LEFS
15 Food microbiology criteria
-Orange highlight=we cant do STAFFS
-Review every 1 year
When bong vin absence, Sopheak reponsible for the criteria of food
Air sampling for Kirirom Ask them to specify:

16 food -Time of sampling Receptionist
Production at KP Speu -Date of sampling
-Payment
Date limit Remarks
Immediately
Jun-20
June-2020
Already Applied
Jul-20
Immediately
Jun-20
Already Applied
Already Applied
Date : 10/06/2020
Time : 10:30 AM
Staff absent : No
Report Written by : HAM Menghor

Person
Problem
Control air in culture media production is overlimit
Root cause
-It may maintenance air conditionner by open emergency door.
1 Environment Control -Sometime Bong Phy doesn’t change shoes before entry room lab Menghor
Solution
-Bong Phy have to change shoes, If he works.
-If Bong Phy just bring equiment or media, keep at out side.
-If maintanance need to clean air conditionner, no need to do anything.
Bong Navin will update citeria for water that separate type water such as wash vegetable, crop spray, feed
2 Water microbiology criteria fish and feed animal Receptionist
3 Citeria of supplement Bong Pheak will check criteria from Thailand Bong Pheak
Problem
Inputting confus data May confuse when in-coming many samples that mix a sample from bottle water Receptionist
Solution
4 -Need to remark on paper
List of physico-chemical parameters Bong Thy explain 10 parameters (Source, risk) such as ammonia, arsenic, choride, free chloride and total Bong Thy
5 chlorine, iron, nitrite, nitrate, pH, total hardness, turbidity.
Date limit Remarks
Immediately
Date : 28/07/2020
Time : 10:30 AM
Staff absent : No
Responsible
Person
Follow up last meeting

-There are 3 staff have written anonymous letter
'-Anonymous letter
-Bong Seyha will go to exam on Friday
1 -Bong seyha drives tuk tuk -
-Fraser 1 & 2 control, Bong pheak will follow up with Bong Vin
-Freser 1 & 2 control
-Supplement criterial does't citerial yet
-Supplement criterial
-Bleeding: Place thick bandage or clean clothes on the wound. If the blood still flow, Please bring
patient to hospital.
first aid traning feedback from bong
2 - Cardiopulmonary resuscitation (CPR): Compress hard and fast in the center of the chest. Bong Thy
Thy
-Choking: Give 5 back blows and Give 5 abdomnal thrusts.
Notice: Be careful on yourself first.
-Have to check and note on 3rd, 5th, 8th and 10th. Which day that have many colonie, record it. All LEFS
3 Record result legionella
-Have to Keep it for view again, If there are any problem. STAFFS
All LEFS
Confirm intestinal Enterococci -Have to record CC for amount colonie to confirm, then Write N for amount of colonie.
STAFFS
4
Autoclave problem Don’t know reason. Menghor
5
Result of IPC security audit from
French embassy Entrance door does't machine scane for authorized people. So, other people can enterace without have
7 permission
Assistant for mangament stock while
Bong Ya change to reception Who respone at production media room have to respone stock room also. In case, next month bong
8 seyha change to product media so, Bong ya will train him.
Similar name of customer Bong neat presente similar name of customer as: Receptionist
'-Hongkong Lang & Hongkong mention
-Komboul handsome 1 & komboul handome 2
9 -Royal Phnom Penh ….
Date limit Remarks
Already applied
Already applied
-
Date : 29/09/2020
Time : 10:30 AM
PROEUNG Seyha, KEO Sophaneth and HAM Menghor, Bun boren
Staff absent : No
Responsible
Person
1 Welcome staff from FSPI project Mr. Bun boren will work at LMA for 6 months ( test on food and water) -
No close the door completely.

-Write nonconfrimity
Prevent active:
All LEFS
2 Incubaton Problem -Check the door incubation every enter and out of lab.
STAFFS
-Alarm every 30minutes.
-Who use PC that have thermo client have to check this program first.
Notice: If there are any problem please inform immediately.
If there are colonies to consider we have to do gram stain all of them.

All LEFS
3 Gram stain If result not yet release, do not allow though away of gram strain.
STAFFS
Notice: If any problem, please inform to Bong Vin.
-Dryslide have to control (+), (-).
All LEFS
Reagent expired Notice: If reagent expired have to contril (-), (+) before use.
STAFFS
4 Bong Vin will inform what reagent use what to contol.
All LEFS
Traceability of water keep using till half October
5 STAFFS
If temperature original of sample keep in room temperature, we have to keep it in room temperature
after testing. All LEFS
Storage sample after testing
If sample in bottle that have many bottle in a typs have mix it together. STAFFS
7 Notice: if sample could spoil, should keep it freeze.
Bong Nita have accompany Bong Seyha to take sample for a few times.
Tuk tuk Driver Tuk tuk
Pastuer will provice who drive Tuk Tuk for 10$ every month.
8
-Problem in process that over time. On 11st September 2020 that have 9 sample. First dilution is okay
but serial dilution is over time.
-Mossel 30 July doesn't note 2nd expired. Media reagent when open have to note open date and 2nd
All LEFS
Audit practice expired date. If expired of reagent cannot react 1 year, we could reduce time of 2nd expired to 6
STAFFS
month. Depend on reagent.
-Forget note result FTM of ED control on paper media preparation.
9
All LEFS
Schedule cleaing Forget to sign
10 STAFFS
-New supplement of BP show halo doesn't clear. All LEFS
supplement of BP out of stock
11 -If supplement of BP have arrive to BP we can use it before result of control realease. STAFFS
-Dilution
1.4 Whole crustacean such as crabs
-crab
-Disinfection surface
-break by hammer and pliers
-Carapace and claws
-sterile
-Homogenizer
-Dilution
1.5.Shelled crustacean flesh
-Amount of required
-Dilution
-Blending
Specific method
1.6 Species of crustacean tail only are consumed
-Disinfection surface
-Break adbomen and crustceans
-Pull the edible part
- Add dilution
-homogenizer
1.6 Echins, holothurian and tunicates
-Amound sample at least 10
-wash under running water
-Put them on steriel tray
-Cut off a piece
-Collect the whole flesh fluid
-Sterile container
-Initial suspension 1 in 3
-Homogenizer
-Dilution 1 in 10
Presentation of SOP fishery Menghor
Presentation of SOP fishery Menghor
Process product
1. Whole Smoke fish
-Sample cut into small
-Homogenzier
Notice: If the whole fish is eaten with skin, Skin shall be included the sample
If the whole fish doesn't eat with skin, Skin shall be excluded the sample
2. Smoke fish fillet and slice with or without skin
-Sample cutting without skin
-Homogenizer
3. Cook or partially cooked gastropods
-Gastopods
-Remove operculum
-Extract the meat
-Initial suspension 1 in 3
-Homogenizer
-Dilution 1 in 10
Frozen fish, crustacean, molluscs, tunicates and echinoderms

1. Fish fillet, large fish piece frozen in block, frozen small part single portion
-Portion test
-Leave in at 18-27C for 60min not over 3h
-Cutting into small
-Homogenizer
Dilution
Notice: Shell crustacean, whole crustacean, flaked crustacean, molluces gastropods, bivalve do as the
12 same method above.
Date limit Remarks
Already applied
Already applied
Already applied
Already applied
Already applied
Already applied
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Responsible Report 2019 - 2020 (Rotation Plan) Responsible Remarks Laboratory of Environment and Food Safety (LEFS)

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Responsible Report 2019 - 2020 (Rotation Plan) Responsible Remarks Laboratory of Environment and Food Safety (LEFS)

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Laboratory of Environment and Food Safety (LEFS)

Responsible report 2019 - 2020 (Rotation plan)

30, October 2019

Topic discussed Action Responsible Date limit Remarks

LEFS Staff meeting report 2020

Responsible Person Date limit Remarks

every 2 week but mayb impossible

7 Bon travaux stmacher Vuthy 31/01/2020

10 Visitor log book Sopheak DONE on the desk infront

Do not allow intern sudents do sample analysis that effected

Maximum 3 persons in a room

Training oceasoft: LEFS will propose at least 2 technicians to participate follow up

1-Pre-anlytique in reception room

Setting temperature on Technician need to follow up temperature of incubator on display with

Verification of method is not pass yet, will repeat Chakriya

To avoid change or edit on the upper part of result from

After validation of his competent

3 Type of sample Classify the type of sample

1. Carefully with the colonie growth on XLD because we have

Frequency : Control every 2 week

Environment During analysis :

9 Reference strain Sub culture the refernce strain form -80 ⁰C

14 Command extern Follow up bong thea Bong thy

16 PH meter HANA Control PH not working Bong thy

2 Excel Form Update

5 Water Analysis Result Record Update

B. Pheak have to make a new folder to put these model

Reama will come to lab on 11/03/2/2020

8 Gas supply security

Phum Ben & Khmer

Ms. Sopheak Next CCQHS

1. Highlight yellow on which parameter that not satisfactory

1. 5 technicians : Pheaktra, Pheakdey, Sreyneath, Chakriya, and Nita.

Staph <100, so dilution to 10-2 and spread 0.1 ml

1 Covid-19 All IPC staff

1. If situation evolution : reduce mode

Sunday: Pheaktra, Neath and Chakriya

1. SOP Non confomity of Sample Pheaktra

1. reduce mode from 6 April 2020

1- April 8, last day of salmonella

6 Sample PFR 06 April 2020

7 COFFEE CONCEPTS 7-8 April 2020

Topic discussed Action Responsible

3. If B Pheaktra still continue work, She will respond with Water

1. SSPI : Screening 100 patients with 2500 samples from Calmet

3. CAPFISH : To define contanminated fish and fish products from

ath, SEM Nita, SOVANN Chakriya, PROEUNG Seyha

Date limit Remarks

Done Sophaneth will drop email inform to customer

Topic discussed Action Responsible

Nita and Seiha should participate in reading plate and confirm

When reading plate: TBX and VRBL (C>300CFU), but FTM: 0;

Nita will work in water chemistry and control media

SEM Nita, PROEUNG Seyha, KEO Sophaneth

Date limit Remarks

KEO Pheakdey, SEA Longsreyneath, Sem Nita, Proeung Seyha

Nita writing non conformity Media EPT contaminate.

Technicain who respond on food and water

Problem : Oxidase Test is not accurate.

Bong Neath will work as receptionist.

Samples need to be stored separately depend on sample types.

Cronobacter spp Testing for