Graphical abstract: Glucose oxidase (GOD) and cytochrome c

(Cyt c) were co-entrapped in the poly(diallyldimethylammonium

chloride)–graphene nanosheets–gold nanoparticles
(PDDA–Gp–AuNPs) nanocomposites modified glassy
carbon electrode. Electron transfer and electrocatalysis of the
novel bi-protein bio-interphase were investigated. The bio-
interphase developed here not only successfully achieved DET
of GOD, but also showed great potential for the fabrication of
novel glucose biosensors with linear response up to 18 mM.
Highlights: â–º A bio-interphase composed of cytochrome c and
glucose oxidase was developed. â–º The electron transfer in
the bio-interphase was investigated. â–º Electrocatalytic
performances of bio-interphase were explored. â–º The bio-
interphase exhibited good electrocatalytic response glucose. -
Abstract: Glucose oxidase (GOD) and cytochrome c (Cyt c)
were co-entrapped in the poly(diallyldimethylammonium
chloride)–graphene nanosheets–gold nanoparticles
(PDDA–Gp–AuNPs) hybrid nanocomposites modified
glassy carbon electrode to prepare a novel bi-protein bio-
interphase. Electron transfer and electrocatalysis of the bi-
protein bio-interphase were investigated in detail. The results
showed that the PDDA–Gp–AuNPs nanocomposites
accelerated the electron transfer between proteins and
electrode. The bi-protein exhibited effective direct
electron transfer (DET) reaction with an apparent rate constant
(k s ) of 2.36 s −1 . The optimal molar ratio and total amount of
Cyt c and GOD in the bio-interphase for DET of GOD was
estimated to be about 3:1 and 1.40 nmol, respectively. The bi-
protein bio-interphase could be used to detect glucose based
on the consumption of O 2 with the oxidation of glucose
catalyzed by GOD. The resulted biosensor exhibits wide linear
range from 2.0 to 18.0 mM. Thus, this study not only
successfully achieved DET of GOD, but also constructed a
novel biosensor for glucose detection
1. Diffusion and mass transfer
CERN Document Server
Vrentas, James S
2013-01-01
The book first covers the five elements necessary to formulate
and solve mass transfer problems, that is, conservation laws
and field equations, boundary conditions, constitutive
equations, parameters in constitutive equations, and
mathematical methods that can be used to solve the partial
differential equations commonly encountered
in mass transfer problems. Jump balances, Green’s
function solution methods, and the free-volume theory for the
prediction of self-diffusion coefficients for polymer–solvent
systems are among the topics covered. The authors then use
those elements to analyze a wide variety
of mass transfer problems, including bubble dissolution,
polymer sorption and desorption, dispersion, impurity migration
in plastic containers, and utilization of polymers in drug delivery.
The text offers detailed solutions, along with some theoretical
aspects, for numerous processes including viscoelastic
diffusion, moving boundary problems, diffusion and reaction,
membrane transport, wave behavior, sedime...
2. Heat and mass transfer
CERN Document Server
Karwa, Rajendra
2017-01-01
This textbook presents the classical treatment of the problems
of heat transfer in an exhaustive manner with due emphasis on
understanding of the physics of the problems. This emphasis is
especially visible in the chapters on convective heat transfer.
Emphasis is laid on the solution of steady and unsteady two-
dimensional heat conduction problems. Another special feature
of the book is a chapter on introduction to design of heat
exchangers and their illustrative design problems. A simple and
understandable treatment of gaseous radiation has been
presented. A special chapter on flat plate solar air heater has
been incorporated that covers thermo-hydraulic modeling and
simulation. The chapter on mass transfer has been written
looking specifically at the needs of the students of mechanical
engineering. The book includes a large number and variety of
solved problems with supporting line diagrams. The author has
avoided duplicating similar problems, while incorporating more
application-based examples. All the end-...