SPECTROPHOTOMETRIC DETERMINATION OF FE(II) AND ZN(I) IN THE PHARMACEUTICAL MULTIVITAMIN PREPARATIONS WITH MICROELEMENTS STANISLAW ZAREBA', ARKADIUSZ POMYKALSKF and ZBIGNIEW MARZEC' * Department of Food and Nutrition, Faculty of Pharmacy, Medical University, 4 Stasviea Str, 20-016 Lublin » Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University, 6 Chodaki Str, 20-093 Lublin, Poland, Abnract: 3-merapto-5-34-dihyonypenylizo-I?}-1.24-trinoe (METRIAP), Srerepto-S-(24-4i byrony-2-crhoxypenylazo-12--taale(METRIAREZ- and 2-revcaio-$-424-dihydroxy-$-ar twnyphenslazo-I)-1,34-thadizole(METIDAREZ-), agents syntesied inthe Deparment of Medicinal CCettisty of Medical University in Lablin, have been sed fo determine Fel) and Zl) in Matera, Centrum, H-Pantoten pharmaceutical livin prepsatons, containing ther tae elerents. 2 (I) with [METRIAREZ- tpi 35, and Fell) with METRIAP and METIDAREZ-B xt pHa10.30 oF 70 conse Soluble in HLO colour chelate compound at a mle ratio of 12 and 13, respectively. Volume sabiity ota af Fl aod ay) completes equal to o8 Krys 1646.18 Kyeranae pin = 14253 1og Kyesecynne = 147. Fell and Za) soliion were abled by wet mineralisation af Matera Conran an HPantoen preparations with concentrated H-SO, and 30% H.O: sled. Spetrophotometie Znill). In case of Fe(UD) ions determination, other components of the pPharmaccutical preparations studied do not inter fere. Fe(I}) can be determined without removing ot ‘masking other ions) In case of Zn(II) determina tion, some other ions are removed as. insoluble hydroxides. Summing up, it can be stated that in the conditions of markings the matrix (dyes) did not hhave any inflyence on the results of the markings of Zn (UP) and Fe(ID, which means that the method can be claimed to be specific. The method of examining the regaining carried out with the use of the method of model alton in numbers $0% and 100% of marked quantities gave the scopes of results for zine from 92.2% to 102.8% and for iron from 98.1% t0 102.2%. The precision of results expressed as a minimum range of confidence at 95% probability level (For 10 determinations in two series a” and ,b" gave the mean values for Fe(l) in Centrum: + 0.297 (METRIAP), # 0.321 (METL DAREZ-8); in. Materna: + 0.225 (METRIAP), +£0:300 (METIDAREZ-B); in H-Pantoten: + 030 (METRIAP), + 0.011 (METIDAREZ-), For dete rminations of Zn (ID by METRIAREZ-- the range of confidence at 95% probability was in Centrum 0.310, in Marerna + 0.232 and in H-Pantoten 0.120. Mean value of the variability coefficient RSD for determinations of Fe (II) by METRIAP was 1.02% and METIDAREZ-B) 1.23%; for Zn () by METRIAREZ-y 1.85% The mean coefficient of correlation (7) betwe en two series of determinations," and bin ease ‘of Fe(ll) by the METRIAP method was 0.9200 and by METIDARE7- 0.9333, the value ofr between METRIAP and METIDAREZ-B was 0.9033. TheSTANISLAW ZAREBA et al. 178 | ovo+ | | TIOOFISOT | cco | 0- | torz=G-mem — oer rt wot | o00F OOCIAE w wo- | z1ooRLSVE | wo- | lo- oon | eo (uw cio F sso (@ sro- | wo~ ceria LW 0100 F aso | oro- | 90+ | ore | <0 ‘GN) 9000 F 890% (@ nes | ero- | oe onsed (1000 F L990 & | we 1) wmoweay | sco+ | loorosrse | zeo+ | w0- sso | Gai 1070 F ono se «@ avez, | sco+ | ay0- Gouin) 9570 ¥ ou0'st ons 0 zo SOC OFFS | | oo+ | zeo+ wo | @tted revo F or09 | uko- | s90~ | | sot | wor «0 | wa wor | s+ 03008 1 suey | { wor | | eezoworsi | ores | eet | toreet-wemm saci | woe UUW) 2160 FOI st cuz 180+ | OvO+ | OBE ALE Ta Geman 130 Fors vost | 10+ sseOFOL'st | zzo- | es0- we0 | sot | oxo+ | soe our't=a ss0- | ott eo | 9st (wd zev0 ¥ 00641 &@ | apr wo- | ev0- exerts OW co¢0 F O96 LI | cost 1 wag svv 5a | enero | ero | | sir = | J nome a 0 Snge fms | aguy= if ‘ome Jom 30 SV. 49 dtget 2pjge 200 | — id 00 UF Bar ty pg 30 | pazetoap tw GAUL) A zeravrettan 6 (7, G-LIYY fl ~ZeRIVOILAWN 9m ON) ARAN 4 CDA F-Pom-EG | one pouMUG | aK JO OMY | Noman wopeoRp OF 2 HD SOs Om! ~ 4" How (SURE Gre 0} QTY) [Ao] ANAT 66 EF VONENEAS ORNS SOME pz, PEE DEA 7 SINS “TSRSpectophotomerc determination of Feil) and Zin pharmaceutical mltianin 179 coetficient of correlation between AAS and Mi RIAP was 0.8733 and between AAS and METIDA. REZ- 0.9000. In determinations of Za (Ul) by METRIAREZ--7the (r) value between two series of. a” and ..b” was 0.9300 and between AAS and METRIAREZ-~y 0.9333. The mean % error for Fe (D in reference to the content declared renged from -0,12 © +0.69% determined by METRIAP, from 0.51 to 40.34% in METIDAREZ-B and from -0.46 to +0.61% in AAS, In reference to the AAS method. it ranged from -I.16 10 40.83% (METRIAP) and from -0.83 to +0.32 (METIDA- REZ-B). In Zn determinations with reference to the content declared, the mean % error ranged from 40.42% to 2.14% (METRIAREZ~)) and. from 146% to 1.46% (AAS) ‘The value of coefficient (F) close to I and the mean value of RSD not higher than 2% and the relative error of determinations, compared 10 the content declared and the content determined by AAS<3%, show that azo—dyes, 1,2.4-triazol deri- vatives (METRIAP and METRIAREZ~/) and 1.3.4triazol derivatives (METIDAREZ-8) can be used fo determine Fe (1D) and Zn (II) content in pharmaceutical multivitamin preparations: conta ring microelements, The studies of repeatibility results showed no significant differences at the assumed confidence level. Even a considerably hhigh value of F (2.92) for the determinations of Fe ID in Materna did not exceed the critical F 0.05 G.18). In reference to widely used AAS method (when compared with the widely used AAS met hhod), the spectrophotometric method characterises itself with a similar marking but a much better precision due to relatively high contents of zine and iron in the examined samples. CONCLUSIONS ‘The advantage of the method developed is the high precision and reproducibility of results of Fe(II) and Zn(ll) determination in. multivitamin preparations with trace elements, such as Centrum, Materna, H-Pantoren, The results of the deter- mination of these metals obtained with METRIAP, METIDAREZ-8 and METRIAREZ~y_ reveal ‘a smaller error. Than those obtained by the AAS method. The examined azo dyes can be successfully used as reagents for the determination of Fe(I1) and Zn(10) simultaneously and together with other trace ele ‘ments present in multivitamin preparations (with trace elements). REFERENCI 1. Salinas F., Mattinez—Vidal LL, Martinez-Ga- lera Mz Quim. Anal. 9, 157 (1990). 2. 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