Process Of Gene Cloning

In the processing of gene cloning,

- Isolated from the gene

- Extracted out DNA sequence is on what interest, this code for particular product
before the multiple copy is that particular product
- Transfer particular DNA into vector, vector are nothing these are just a cariot and
they have all these others vectors
- DNA need to insert into particular vectors

Recombinant DNA

- Transfer particular recombinant DNA into the host cell, and those host cell could be
any bacteria and we taking for example that is eagle eye
- Transfer into eagle eye
- In the eagle eye that particular is numerous copies. Multiple copies of gene of interest

For recombinant DNA formation

- DNA manipulative enzymes

 Restriction endonuclease
 DNA ligase, because DNA is cut to the fragment we need into join
 DNA polymerase
- Vectors
Just carrier that particular recombinant DNA and transfer into the host cell
- Host cells

Produce particular DNA, particular gene and multiple copies