NAMA : ANGGITA FAUZIAH SADILA PANE

NIM : 170301062
KELAS : PEMULIAAN TANAMAN 2018
MATKUL : KULTUR JARINGAN

REVIEW JURNAL INTERNATIONAL

TOPIK : PENGARUH ZPT TERHADAP KULTUR HAPLOID
JURNAL 1
Judul The Effect of Different Levels and Sources of Auxin and Cytokinin to
Callus Formation on Soybean Anther Culture
Jurnal Journal agrotek.Trop
Volume & Halaman Vol 3(2) : 58-67
Tahun 2014
Reviewer Anggita Fauziah Sadila Pane
Tanggal 11 Oktober 2020
Tujuan Artikel Jurnal This investigation was aimed at studying the effect of types and
concentrations of auxins and cytokinins on the growth and development
of anthers of two soybean cultivars, Merubetiri and Wilis cultured in
vitro.
Inti dari Jurnal  The trial was conducted at the Plant Biotechnology Laboratory,
Agricultural Faculty, University of Jambi.
 The experiment was placed in a completely randomized design
with five replicates. Each replicates consisted of 8 to 10 anthers
obtained from the same floral bud.
 Cultures were placed in a light intensity of 50 µmol m-2.s-1 and
16-hour photoperiod at 25±1 o C. Observation was done weekly
for 8 weeks of culture.
 Results indicated that response showed by anthers cultured on
medium supplemented with 2,4-D+BAP, IAA+BAP and
NAA+BAP, in the form of callus proliferation, occurred within
5-18 days of culture initiation. Meanwhile, with 2,4-D+kinetin,
IAA+kinetin and NAA+kinetin, callus proliferation took place
within 4-16 days of culture initiation. Callus formation was
preceded by a swollen on the surface of anthers, followed by
 changing in color from light green to brownish. Following this,
anther wall turned into amorphous shape, before it was finally
covered by a white, cream or light green callus mass. Initially,
the callus showed friable or compact structure, but following
two weeks of proliferation all callus showed compact structure.
Among growth regulators tested, combination involving 2,4-D
produced more callus than other combinations. In addition, of
the two cultivars tested, Merubetiri showed better response
compared to Wilis.
Metode Penelitian The experiment was placed in a completely randomized design with five
replicates. Each replicates consisted of 8 to 10 anthers obtained from the
same floral bud.
Hasil dari Jurnal Anthers from the two soybean cultivars (Merubetiri and Willis) cultured
on solid MS medium supplemented with IAA, NAA or 2,4-D in
combination with BAP or kinetin showed callus formation within 5-18
days after culture. Callus proliferation was started by swollen on the
anthers surface followed by changes in their colour from light green into
brownish. Following from this, the anther wall showed amorphous
structure, and finally the whole anthers were covered by callus mass.
Meanwhile, on non callus forming explants the colour of anthers turning
into white or brown and show no further development.
Kesimpulan 1. The presence of plant growth regulators, particularly IAA, NAA
or 2,4-D and BAP or kinetin, in culture medium was crucial in
promoting callus formation on anther culture of soybean, either
on Merubetiri or Wilis cultivars. However, Merubetiri was found
to produce better in vitro response compared to Wilis.
2. Among tested auxins and cytokinins, the combination of 20 µM
2,4-D with 10 µM to 20 µM kinetin showed better results than
other combinations.
3. Based on this reported investigation, future study should focus
on in vitro culture of Merubetiri along with the use of 2,4-D and
kinetin.
JURNAL 2

Judul Influence of growth regulators on callus induction and plant

regeneration from anthers of Tagetes spp.
Jurnal Indian Journal of Agricultural Sciences
Volume dan Halaman 88 (6): 970
Tahun 2018
Reviewer Anggita Fauziah Sadila Pane
Tanggal 11 Oktober 2020
Tujuan Artikel Jurnal the objective of the present investigation was to study the effect of
growth regulators for in vitro regeneration in both African
marigold and French marigold genotypes viz., Pusa Basanti
Gainda and Pusa Arpita, respectively for haploid induction.
Inti dari Jurnal  The present experimentation was carried out at the Central
Tissue Culture Laboratory, ICAR-National Research
Centre on Plant Biotechnology, Pusa, New Delhi during
2014-2017.
 In the present study, the structures of the anthers were
found to be similar in both the species. The anthers were
inflated after one week on the induction medium
 That media devoid of growth regulators (control) showed
maximum non-responsive anthers over all other
treatments. Among the different treatments, least (42.75%)
number of non responding anthers were reported on MS
medium supplemented with 1.0 mg/l BAP + 1.0 mg/l 2,4-
D, which was significantly different with MS + 2.0 mg/l
BAP + 1.5 mg/l 2,4-D (47.32%) and MS + 2.0 mg/l BAP +
1.0 mg/l 2,4-D (47.57%).
Metode Penelitian Among the different treatments tested for callus induction, anthers
cultured on MS medium supplemented with 1.0 mg/l BAP + 1.0
mg/l 2,4-D + 45 g/l sucrose significantly induced highest
(38.74%) callus in the shortest period of time (16 days). Among
two genotypes tested, embryogenic callus induction (27.70%) was
significantly higher in Pusa Arpita over Pusa Basanti Gainda
(23.37%). Among the different regeneration treatments, the
highest adventitious shoot bud induction (13.32%), maximum
(1.09) number of buds/callus in shortest duration (19.47 days) was
observed in anther derived callus cultured on MS medium
supplemented with 2.0 mg/l BAP + 0.5 mg/l NAA + 30 g/l
sucrose. Among two genotypes evaluated for shoot bud induction,
maximum (3.81%) regeneration was recorded in Pusa Arpita over
 Pusa Basanti Gainda (2.90%).
Hasil dari Jurnal Among the different treatments tested for callus induction, anthers
cultured on MS medium supplemented with 1.0 mg/l BAP + 1.0
mg/l 2,4-D + 45 g/l sucrose significantly induced highest
(38.74%) callus in the shortest period of time (16 days). Among
two genotypes tested, embryogenic callus induction (27.70%) was
significantly higher in Pusa Arpita over Pusa Basanti Gainda
(23.37%). Among the different regeneration treatments, the
highest adventitious shoot bud induction (13.32%), maximum
(1.09) number of buds/callus in shortest duration (19.47 days) was
observed in anther derived callus cultured on MS medium
supplemented with 2.0 mg/l BAP + 0.5 mg/l NAA + 30 g/l
sucrose. Among two genotypes evaluated for shoot bud induction,
maximum (3.81%) regeneration was recorded in Pusa Arpita over
Pusa Basanti Gainda (2.90%). In French genotype, the whole
regeneration process was completed in 30 days whereas in African
genotype it took nearly 40 days. This rapid regeneration system
from anthers is highly useful in inducing haploids and di-haploids
in African and French marigold lines, respectively.
Kesimpulan From the present studies, it is concluded that the current protocol
can be effectively used for haploid induction and genetic
manipulation for improvement of marigold species. Considering
the findings, further investigation is required for enhancing the
callus induction, plant regeneration and haploid production of
marigold by experimenting with the type of basal medium, pre-
treatment temperatures, concentration of carbohydrates and
various other factors responsible for androgenesis in marigold.