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CLINICAL ARTICLE
Effect of integrated yoga on stress and heart rate variability in pregnant women
Maharana Satyapriya a, Hongasanda R. Nagendra a, Raghuram Nagarathna a,⁎, Venkatram Padmalatha b
a
Division of Yoga and Life Sciences, Swami Vivekananda Yoga Research Foundation, Bangalore, India
b
Maiya Multispeciality Hospital and Wockhardt hospitals, Bangalore, India
a r t i c l e i n f o a b s t r a c t
Article history: Objective: To study the effect of integrated yoga practice and guided yogic relaxation on both perceived stress
Received 20 August 2008 and measured autonomic response in healthy pregnant women. Method: The 122 healthy women recruited
Received in revised form 30 October 2008 between the 18th and 20th week of pregnancy at prenatal clinics in Bangalore, India, were randomized to
Accepted 14 November 2008
practicing yoga and deep relaxation or standard prenatal exercises 1-hour daily. The results for the 45
participants per group who completed the study were evaluated by repeated measures analysis of variance.
Keywords:
Heart rate variability
Results: Perceived stress decreased by 31.57% in the yoga group and increased by 6.60% in the control group
Normal pregnancy (P = 0.001). During a guided relaxation period in the yoga group, compared with values obtained before a
Stress practice session, the high-frequency band of the heart rate variability spectrum (parasympathetic) increased
Yoga by 64% in the 20th week and by 150% in the 36th week, and both the low-frequency band (sympathetic), and
Yogic relaxation the low-frequency to high-frequency ratio were concomitantly reduced (P b 0.001 between the 2 groups).
Moreover, the low-frequency band remained decreased after deep relaxation in the 36th week in the yoga
group. Conclusion: Yoga reduces perceived stress and improves adaptive autonomic response to stress in
healthy pregnant women.
© 2008 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
1. Introduction An ancient holistic Indian science, Yoga, has been used for centu-
ries for stress reduction and health promotion [7]. Guided relaxation
Pregnancy is a state of physiological stress that necessitates physical, techniques based on Yoga and isometric relaxation have been linked to
mental, and social adaptation. Animal experiments and human studies reduced sympathetic activity [8,9]. The practice of cyclic meditation
have shown prenatal maternal stress to be associated with increased was found to reduce oxygen consumption and breathing frequency
risks of spontaneous abortion, preterm labor, fetal malformations, and [10]. Studies on heart rate variability (HRV) during vipassana (mind-
asymmetric growth restriction [1]. In a 2005 study, Weinstock [2] noted fulness) meditation showed a shift toward vagal dominance in the
that blood flow though the uterine arteries of very anxious women was autonomic balance [11].
impaired by the 32nd week of pregnancy, a finding that strongly Positive effects of integrated yoga modules have been demonstrated
correlated with increased plasma cortisol levels in both mother and even in women with high-risk pregnancies [12], but no studies have
fetus. These 2 conditions may have a direct effect on the development of looked at the effect of yoga on perceived stress levels and autonomic
the fetal brain, as permanent alterations in early neuroendocrine responses during pregnancy. The objective of the present study was to
programming are likely to affect responses to stress later in life, assess levels of stress—both as perceived by the participants and from
fostering such disorders as attention deficit, hyper anxiety, and measuring HRV indices—as the response of the autonomic nervous
disturbed social behavior [3]. These disorders are due to abnormal system to yoga modules integrating a yogic-guided deep relaxation
activity in the maternal sympathetic-adrenal-medullary system and technique (DRT).
hypothalamic-pituitary-adrenocortical axis [4].
Studies of adaptive responses of the maternal autonomic nervous 2. Materials and methods
system to pregnancy have noted that the sympathetic regulation was
facilitated and the parasympathetic influence on the heart rate was A total 122 pregnant women satisfying the selection criteria were
attenuated from the 11th to the 27th week [5], and that responses to recruited from a pool of 300 women receiving prenatal care at various
exercise stress were blunted in late pregnancy [6]. Generally, reactivity obstetric units in the south side of Bangalore, India. The recruited
to physiological stress appears to be dampened during pregnancy [4]. women, who were aged 20 to 35 years and between the 18th and 20th
weeks of pregnancy, were randomized to the yoga or the control group.
Final data were available for 90 of these women, 45 in each group.
⁎ Corresponding author. Division of Yoga and Life Sciences, Swami Vivekananda Yoga
Research Foundation (Yoga University) # 19, Eknath Bhavan, Gavipuram Circle, K.G.
The optimum sample size of 44 participants per group was
Nagar, Bangalore, India 560019. Tel.: +91 9342554703; fax: +91 80 26608645. calculated by fixing the probability of a type 1 error at an alpha of 0.05,
E-mail address: rnagaratna@gmail.com (R. Nagarathna). to yield 0.80 power for an effect size of 0.54. The effect size was
0020-7292/$ – see front matter © 2008 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
doi:10.1016/j.ijgo.2008.11.013
M. Satyapriya et al. / International Journal of Gynecology and Obstetrics 104 (2009) 218–222 219
calculated by the Cohen formula [13], using the means and standard taining a computer-generated random number that allocated them
deviations of an earlier interventional study. For these calculations we to the yoga group or the control group. Assessments of stress (from
used the noncommercial statistical power analysis program G⁎Power a perceived-stress scale and HRV) were done between the 18th
[14]. The selection criteria aimed at avoiding conditions that would and 20th week and during the 36th week. For HRV computing, an
heighten the adaptive responses to stress during pregnancy. The electrocardiogram (ECG) was recorded continuously for 5 minutes
exclusion criteria were the following: multigravidity when the before, 10 minutes during, and 5 minutes after the DRT period in the
participant had no living child; multiple pregnancy; maternal physical yoga group and supine rest (SR) in the control group. The yoga group
abnormalities; psychiatric problems; pregnancy-associated medical successively practiced 2 modules of integrated yoga, specifically
problems such as diabetes and hypertension; pregnancy from in vitro designed for the second and third trimesters of pregnancy. The control
fertilization; intrauterine growth restriction in a previous pregnancy; group practiced standard prenatal exercises. In the first month both
fetal abnormality on ultrasound scanning; and previous exposure to groups learned the movements (in exercise routines of 4 to 10) from
yoga. Besides having a pregnancy duration between 18 and 20 weeks, trained instructors, in 2-hour sessions 3 days per week. After 1 month,
the inclusion criterion was primigravidity, or multigravidity when the the participants continued to practice for 1 hour at home using a
participant had at least one living child. The ethical committee of Yoga prerecorded instruction cassette. Both groups also had 1-hour re-
University (SVYASA) approved the project. Signed informed consent fresher classes each time they came for their prenatal visit, once every
was obtained from all participants before randomization. 4 weeks up to the 28th week and every 2 weeks up to the 36th week.
This was a prospective, randomized, 2-arm study in which all Compliance for completing the 60-minute practice was ensured by
participants engaged in supervised exercise practices from the time of telephone calls and the maintenance of an activity diary.
recruitment until delivery. After they were screened by the chief As this was an interventional study, the participants and trainers
obstetrician of their prenatal clinic and had provided signed informed could not be blinded, but the team who did the assessments and the
consent, the participants were assigned a numbered envelope con- statistician were blinded. The perceived stress scale (PSS) answer
Table 1
The 60-minute daily practices used by the 2 groups
sheets were coded and kept away until the end of the study, and care Table 2
was taken to prevent the participants from discussing the techniques Maternal Characteristicsa
3. Results
The trial profile is shown in Fig. 1. Data for 90 women were available
for final analysis. The reasons for dropping out of the study included the
inability to attend the prenatal care clinic regularly; switching from the
control group to the yoga group because of the increasing popularity of
yoga through the media; and leaving Bangalore, or the in-laws' house for
delivery and postpartum care in their parents' house.
Demographic characteristics are shown in Table 2. The 2 groups were
matched for all baseline characteristics excluding professional status
(i.e., housewife or working outside the home). Obese women were
Fig. 1. Trial profile. excluded but not overweight women (body mass index [BMI, calculated
M. Satyapriya et al. / International Journal of Gynecology and Obstetrics 104 (2009) 218–222 221
Table 3 Table 5
Changes in perceived stress after intervention in both groups (n = 45 in each)a Changes in HRV before and after the DRT and SR periods in the 20th and 36th weeks of
pregnancy in the yoga and control groups (n = 45 in each group)a
Variable 20th wk 36th wk ES % of P valueb
change Within Between groups Week HRV Group Pre- session Post- session Pre-post % ES P value
groups Mean ± SD Mean ± S.D Pre-post Pre-post
Pre-post Pre-pre Post-post 20th LF DRT 74.76 ± 5.87 74.05 ± 6.64 - 0.9% 0.15 ns
SR 73.31 ± 7.95 70.51 ± 11.55 - 3.8% 0.32 ns
PSS
HF DRT 25.23 ± 5.87 25.95 ± 6.63 2.9% 0.15 ns
Yoga group 15.90±5.01 10.88 ±4.97 0.29 -31.57 0.001 NS 0.001
SR 26.74 ± 7.96 29.39 ± 11.60 9.7% 0.31 ns
Control group 15.43±5.70 17.33 ±5.34 0.26 6.60 0.002
LF/HF DRT 3.51 ± 0.21 3.07 ± 0.95 - 12.5% 0.50 ns
Abbreviations: ES, effect size; PSS, perceived stress scale. SR 2.89 ± 0.83 2.81 ± 1.15 - 2.8% 0.07 ns
a th
Values are given as mean ± SD or number unless otherwise indicated. 36 LF DRT 76.31 ± 4.85 72.51 ± 6.00 - 5.0% 0.63 0.001
b SR 74.17 ± 9.53 70.86 ± 7.44 - 4.5% 0.33 ns
By repeated measures analysis of variance.
HF DRT 23.24 ± 4.91 27.52 ± 5.98 18.4% 0.78 0.001
SR 25.04 ± 7.20 29.13 ± 7.44 16.3% 0.49 0.006
LF/HF DRT 3.46 ± 0.78 2.79 ± 0.75 - 9.4% 0.83 0.001
SR 3.20 ± 0.86 2.64 ± 0.90 - 17.5% 0.61 0.001
as weight in kilograms divided by height in meters squared] was less Abbreviations: see Table 4.
a
than 25.6 in both groups). Values are given as mean ± SD or number unless otherwise indicated.
b
The PSS scores are shown in Table 3. The mean baseline scores P b 0.001.
c
P = 0.015 between groups (DRT and SR) by repeated measures analysis of variance.
were 15.9 in the yoga group and 15.4 in the control group, and are
marginally higher than the mean scores (14.1) in India. The mean
perceived stress was reduced by 31.57% in the yoga group and There were no significant correlations between PSS scores and any
increased by 6.60% in the control group, and the difference was sig- of the HRV values, either in the 20th or in the 36th week.
nificant (P = 0.001).
Values for the components of the HRV spectrum (LF, HF, and the LF/ 4. Discussion
HF ratio) during the DRT period of the yoga module (yoga group) and
the SR period of the exercise session (control group) are shown in This prospective, randomized, controlled study compared the
Table 4. Compared with the values obtained before the session, the LF effect of daily practice, from the 20th to the 36th week, of an
band power (a measure of sympathetic tone) and the LF/HF ratio integrated yoga program (n = 45) and a program of standard prenatal
decreased and the HF band power increased during both DRT and SR exercises (n = 45) in healthy pregnant women. The perceived stress
(P b 0.001), with a significant difference between the 2 groups decreased by 31.6% in the yoga group and increased by 6.6% in the
(P b 0.001). In the 36th week, compared with the values obtained control group, and the difference was significant between (P b 0.001).
before the session, the degree of response to DRT was increased Measurements of the components of the HRV spectrum showed that
significantly in the yoga group whereas the degree of response to SR the sympathetic tone (LF band power and LF/HF ratio) decreased, and
was reduced in the control group. the parasympathetic tone (HF band power) increased during both DRT
The corresponding values after the DRT period of the yoga module and SR periods in the 20th week. In the yoga group, the changes in
(yoga group) and the SR period of the exercise session (control group) HRV values during and after the DRT period were significantly
are shown in Table 5. In the 36th week, compared with the values enhanced in the 36th week.
obtained before the session, the LF band power was significantly A 64% increase in the HF band power during DRT in the 20th week,
reduced after the DRT period (P b 0.001) but not after the SR period. and a 150% increase in the 36th week, demonstrate that the regular
Moreover, the reduction in the LF/HF ratio and the increase in the HF practice of the integrated yoga modules used in this study, with their
band power, which were not significant in the 20th week, were guided yogic relaxation, improves parasympathetic tone. The con-
significant after both DRT and SR in the 36th week (P b 0.001), comitant reduction in LF band power and LF/HF ratio, indicating
indicating improved autonomic response after both practices. reduction in sympathetic tone and increased autonomic balance,
corroborate the beneficial effect of these yogic practices on the auto-
nomic nervous system.
There was no change from the 20th week to the 36th week in the
Table 4 LF band values (sympathetic) or HF band values (parasympathetic)
Changes in HRV before and during the DRT and SR periods in the 20th and 36th weeks of
pregnancy in the yoga and control groups (n = 45 in each group)a
obtained before the yoga or standard exercise sessions, which may be
due to the natural dampening of autonomic responses, an expected
Week HRV Group Pre During Pre- ES P value phenomenon as pregnancy advances [4]. In the yoga group, the
during, % Pre/during Pre/during
reduction in sympathetic tone (with an increase in parasympathetic
20th LF DRT 74.76 ± 5.87 58.64 ± 11.72 – 21.6 1.74 0.001
tone) during the DRT period was significantly higher in the 36th than
SR 73.31 ± 7.95 40.84 ± 15.42b – 44.3 2.65 0.001
HF DRT 25.23 ± 5.87 41.35 ± 11.73 63.9 1.74 0.001 in the 20th week. In the control group, compared with the values
SR 26.74 ± 7.96 59.28 ± 15.35b 121.7 2.66 0.001 obtained in the 20th week, these responses were dampened in the
LF/HF DRT 3.51 ± 0.21 1.59 ± 1.07 – 54.7 2.49 0.001 36th week. This finding shows that yoga was better than standard
SR 2.89 ± 0.83 0.92 ± 0.87b – 68.2 2.32 0.001 prenatal exercises in improving autonomic nervous system response
36th LF DRT 76.31 ± 4.85 41.97 ± 12.17 – 45.0 3.71 0.001
in the third trimester of pregnancy.
SR 74.17 ± 9.53 51.23 ± 14.03b – 30.9 1.91 0.001
HF DRT 23.24 ± 4.91 58.02 ± 12.17 149.7 3.75 0.001 Yogic relaxation techniques are known to improve sympathovagal
SR 25.04 ± 7.20 49.44 ± 15.15b 97.4 2.06 0.001 balance by reducing the sympathetic tone in normal volunteers.
LF/HF DRT 3.46 ± 0.78 0.88 ± 0.49 –74.6 3.96 0.001 Guided relaxation has been shown to be more effective in reducing
SR 3.20 ± 0.86 1.23 ± 0.80c – 61.6 2.37 0.001
physiological arousal than supine rest [24]. Vempati and Telles [8]
Abbreviations: DRT, guided deep relaxation technique; ES, effect size; HF, high showed a significant reduction in sympathetic activity, with a de-
frequency; HRV, heart rate variability; LF, low frequency; NS, not significant; SR, crease in the LF band power and increase in the HF band power
supine rest.
a
Values are given as mean ± SD or number unless otherwise indicated.
during DRT, as well as a significant reduction in breath rate, volume of
b
P b 0.001. air inhaled, and oxygen consumption [9]. The stress reduction and
c
P = 0.015 between groups (DRT and SR) by repeated measures analysis of variance. healthier autonomic responses observed in this study may point to an
222 M. Satyapriya et al. / International Journal of Gynecology and Obstetrics 104 (2009) 218–222
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We thank SVYASA University for funding this project.
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