Week 13_Complexometri Indirect titration Anionic compounds (barbiturates - e.g. barbituric acid) are incompatible with EDTA, therefore they can’t be titrated directly. Then this method is used Procedure: 1. The analyte solution containing the anionic compound is taken in a conical flask. This compound can act as a complexing agent 2. A specific metal (say Hg?*) solution is added, The metal ion will form complex with the analyte compound at 1:1 ratio and be precipitated. The dition of metal solution is stopped when a clear solution is obtained and. precipitation is stopped. Addition of further Hg?’ would give faulty value later during calculation ed OO - —-Week 13_Complexometri... Indirect titration Hg-B complex Excess amount of EDTA is added. It forms complex with the Hg2+ replacing the anionic analyte compound. The amount of EDTA added is known. Since excess was added, some EDTA wall remain free after complexation with Hg’* EDTA + H9-8 = = B + Hg-EDTA + EDTA (ex omp (free 4. A suitable indicator was added. § Another known conc. metal solution (ZnSO, solution) was added. Zn* will form complex with the excess free EDTA es os e -—-6:56 rig arr € Week 13 Complexom.. E&| Indirect titration Zn** + EDTA —————=>_ Zn-EDTA (free) complex 6. When all the free EDTA is complexed with Zn2+, Zn2+ will form complex with indicator and this is the end point IE F 2n** + Indicator === Zn-Indicator (free) complex Step 3-6 actually describes procedures of back titration (olocy] 17:38Cd 7 0.00 AT RTT Rosle) xen GD < Week 13_Complexom.. © 17:38 09:40eer ATT Rohs YA 4g pci € Week 13 Complexom.. E&| 1ethods of end point detection There are several methods suitable for the determination of end point in complexometric titration. These methods are classified as 1. Visual methods: This method is widely applied a. Metallochromic indicators b. pH indicators c. Redox indicators 2. Instrumental methods: If the solution is highly coloured or dilute then visual method may give inaccurate result. Then the following methods are utilized a. Potentiometric method b. Photometric method Toh 17:380.00 4g, perms ZB < Week 13_Complexom.. © pH | Metals Complex | range | detected i 67 | Ca Ba Mg, Zn, Cd, Ma. Pb Hg Pb, Zn |_ Ca, Hg Ca, Cu, Co Pb,Zn Ca, Hg Pb,Zn | Ca. Hg Mg, Mn Fe, Co, Pb @ 13:50 17:38 il y, ord 6:58 raed < Week13 Complexom.. El : thods of end point detection These indicators are organic compounds (usually dyes) which show one color in the free state and another distinct color when complexed with free metal ions The sequence of reactions that leads to end point detection by this method is given below- -EDTA Me? + EDTA === MEDTA complex (different color st of the metal free EDTA first for with free me it replace m M-l 15:29 17:38rd ATT Resets} Ad ces € Week 13 Complexom.. E&| hods of end point detection 1. First when indicator is added M-I complex is formed. But most of the metal ion is free m4 1 M4 complex ane 2. EDTA is added. First it will consume the free metal ions and M-EDTA complex is formed mM" + EDTA = MEDTA complex 3. Then EDTA will replace indicator from the M-I complex. M-1 EDTA M-EDTA complex complex 4. The free indicator shows different color TCHCKS) 17:38 il () u