Production of Polyhydroxyalkanoates (PHA)

For production of Polyhydroxyalkanoates (PHA), the nutrient source used can be any carbon
source like Food waste, Industrial Waste Water, any Sugar, Fatty Acids etc.
Here the nutrient source considered is Fatty Acids. The simple description of the production
route is as follows;
The Coenzyme A (CoA) binds the fatty acid and get converted to an AcylCoA. AcylCoA gets
dehydrogenated and gets converted to Enoyl CoA. EnoylCoA binds to an Enzyme (PhaJ)
which hydrolyses EnoylCoA to (R)-3-HydroacylCoA. (R)-3-HydroacylCoA acts as substrate
for the final reaction which gives PHA as product. (R)-3-HydroacylCoA binds with a
Synthase enzyme (PhaC) and gives PHA as product.
Fatty Acid + CoA AcylCoA
AcylCoA + AcylCoA Dehydrogenase EnoylCoA
EnoylCoA + PhaJ (R)-3-HydroacylCoA
(R)-3-HydroacylCoA + PhaC [(R)-3-HydroacylCoA-PhaC] PHA + PhaC

(k1 / k-1) (kcat)

[S] [E] [E-S] [P] [E]

Michaelis-Menten Equation is given as;

𝑣 [𝑆]
𝑣=
[𝑆] + 𝐾
Where,
v is rate of reaction in [moles/(litre.s)]
vmax is maximum rate reached. vmax = [E]t*kcat
[E]t is total enzyme concentration taken in (moles/liter)
kcat is reaction rate constant of reaction giving PHA
[S] is substrate concentration in (moles/liter)
Km is Michaelis-Menten Constant