1. There is a relationship between concentration and absorbance.

This relationship is expressed by

the Lambert-Beer law, which is more commonly known as Beer's law. This law states that the
absorbance of a light absorbing material is proportional to its concentration in solution.
2. Serial Dilutions Enable Production of Samples with Known Concentration. Clearly, accurate
pipetting during preparation of serial dilutions is critical, because any deviation will propagate to
all of the subsequent steps. Furthermore, the precision and accuracy of serial dilutions depend
on thorough mixing at each step.
3. Wavelengths between about 450 and 495 nanometers.
4. Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of
radiation, as a function of frequency or wavelength, due to its interaction with a sample. The
sample absorbs energy, i.e., photons, from the radiating field. The transmitted energy can be
used to calculate the absorption.
5. The higher the absorbance of light by a solution, the lower the percent transmittance. The
wavelength at which absorbance is highest is the wavelength to which the solution is most
sensitive to concentration changes It ensures highest sensitivity and minimize deviations from
Beer's Law.