Blogensoes AND TNsTRUMENTATION SENSORS 1 Atle’ MNIT TEST— & Name: S: Yoshuonth Utuno. 12200 o . Fooutty = Dr‘Toyantss potantuel Scanned with CamScannerClassiticatton of the btologteal recogettion elements & Signal transducers employed tn Blosensor deuetoprae nt Blotogient RecoGMITION Clement _ gi@uartRAnsDLee ; ! | Enxyrnes } ete EMICAL Cpartbind Berade fetus; single mantp SReces Ror kil | Potentionnetst¢ | *Catalytle tansFormation of the -malyte into ‘amperomett as ~ ke be tngor -~detectoble product conductance “The Analyte fnhtbils the enrymatte acuity, | copacitative tA datectable characterstic of enzyme ts changed upon Interaction With-Analyte- | | Antt bodies | Antigens OPTICAL i| Based on the spectete & high aFrintty antibody Absortance | antagen binding ‘interactions A tracer Coen | d to neratt asigno|> | [used fo generar asiqnol Luninescent Nucleic Actds: Memon . Detection oF DNA-ligand (pollutant) ‘interact’. Index | | ons 2 | Detection oF specific DNA sequences by Might seatte- | | hybridisation . ng [eeltutor Structures BWhokoeiss SSC | | Whole Celis: | | *Pottutont dependent Yncrease tm microorganism Mass S@nistTue | | | oer Pleroetectric || | + Pottutant dependent tnhtbrtton of microorganism) — SUrFaae— P 4 : respi raton: Acoustic Non. enzyme proteins: + Reipters, spedatty inturoreceptors, a class | oF protel ns Ithok ‘usualy contain one & more gelecttue bindt Teese product a gignal through o transmembrane ionchanndd gecondary mes sengen eneqrnt eavuation s13is ~ Scanned with CamScanner© Cxtro cellutar, membrane & Fntracettuta } transport Protetns + Others: lectins Blom Mente REcePTORS “Gunetoanty tng ineered molecules * RNA & ON v aovares + Artista membranes * Moleautary imprinted polymers Pla coelectie | SurPace acouste | THeRMOMETRIC | thermistor Pyroetectrie | | | optical enthalpintter- | | Scanned with CamScannerS) | | | | | Trmobilization modes: DNA-Ads DNA-DTC DNA-SH SPR Pris tT Methodology dripptng oF asp © The tramobilizatton Was accomplished by odes & Spri or each Solution on Freshly cleaned gotd electr +ronsductrs* 2 The topography uios oF sprt blochtp typtcatty conststs thret seperate uertical Sttps: Tre tnerease OF dieFerentt the response to complementary the concentration OF SRM» regard shotegy + Resuit oF Individual experiment ndteattNg the copabiltty oF pna-pTe For hybrtdt zation wath complenentaryé equenct, together udtth the spuirted seeps oF spet expertmment dcpicted tn giuen diagram a\ sprt signal las observed tn target sequence used 19 ess OF trnmobilizati on Scanned with CamScanner*Blrse, the reasa bt lity OF obtained receptor losers was Uertered by Four Fold repetition oF target DNA Injectton(sonm), each time Fotlourd by removal cr hubridteed Strand using bommM oF Nootl solution 1 the igentration step: Tn astudted case the seattstcaly relevant drop oF Spet response along wath subsequent “yq eneration cy cits Las not obserted: © Such result Sndtoates the ‘releuance OF non-speeFr ce interantions blw oltganucleoticles & god surkaca: TRE suxpristng oobustness oF tats construct can bt ontqnd +0 inercased stgritieance oF muttidentate, adsorpiut \nteractions oF amint moieties oF nutteobases oboserued for DNA Probes OF moderate length: 1 The: mechanisens OF length § base dependent 5S DNA adsorption on qe wort thoroughly inuestigated Main Appitcations [The approach woas utilized For development OF DNA biosensors & multi plex mi croplat forms. a: Asd DNA adsorptive approach used to surrtcrent to assure the Stable Attachment oF DNA receptors for Father studtes oF mtcrobluiddte systern - Scanned with CamScanner