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Procedure
SAFETY:
Investigation
QUESTIONS
1 It is important to add the buffer to the enzyme before adding the starch.
Why is this?
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2 How does a control help you with the colour comparison?
4 Do you think your results are reliable? How would repeating the
investigation help you to check the reliability?
8 Describe how you would modify this activity to investigate the effect of
temperature on the rate of break down of starch by amylase.
2 A control makes it easier to be sure that all the starch is gone as it shows
you the colour of the iodine solution diluted with a drop of water (which we
are sure contains no starch).
4 The results are reliable if they are consistent from one group to another, or
if one repeat after another gives a very similar result.