unacademy Welcome to Unacademy Question - Answer on Animal cell culture & Regulations LESSON 10F1 Download the Unacademy Learning App to watch this and over 200k more lessons in UPSC, SSC CGL, GATE, Google Play ff @ App Stor CAT and many more categories.GAYATRI KUMARI * BSc triple major in Biotechnology, Botany and Chemistry -Educator at Unacademy -Painter and Dancer -Yoga practitioner ‘Rate, Review and Recommend. -Follow me on: https://unacademy.com/user/Gayatrikumari- 3606 -Comment box below welcomes all your questions.YF unacademy 1. For culturing, plasma from the adult chicken is preferred to mammalian plasma because A. it forms a clear and solid coagulum even after dilution it is too opaque it doesn't produce solid clots it forms a semi solid coagulum'@ unacademy 2. Toxicity due to accumulation of ammonia can be overcome A. by substituting glutamine by glutamate B. by controlled addition of glutamine at low level c. by removal of ammonia or ammonium from culture medium all of the above4. Disaggregating of cells can be achieved by A. physical disruption B. enzymatic digestion C. treating with chelating agents D. all of the above' unacademy 5. Accumulation of lactate leads to A. B. increase in pH no change in pH reduction in the pH of culture hence loss of cell viability no loss of cell viabilityYF unacademy 6. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of A. incomplete glutamine oxidation B. increase in specific lactate production from glucose C. both (a) and (b) accumulation of ammonia'@ unacademy 8. Excess CO suppress cell growth and productivity by A. inhibiting respiration altering intracellular pH by diffusing across cell membrane both (a) and (b) altering pH of the medium'F unacademy 9. Which of the following is incorrect? A. Established cell lines (ECL) have short doubling time ECL are invariably aneuploid ECL grow in higher density ECL do not show much evidence of spatial orientation'@ unacademy 10. What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? A. B. They act as growth promoters They act as growth inhibitors Have no effect on cells Lactate helps in the growth while ammonium inhibits the growth'F unacademy 11. What is the concentration of CO2 required for culturing animal cells? A. 2-5% B. 1-10% Cc. 10-15% D. 15-20%'F unacademy 15. What are the main constituents of culture for animal cell growth? A. B c. D Glucose and Glutamine Growth factors Cytokines All of these‘Y unacademy 16. During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? A. Ethyl alcohol is being produced in excess B. The cells have too much oxygen C. Glycolysis is being inhibited D. The cells do not have enough oxygen‘P unacademy 19. pH of culture medium is initially controlled by A. B c. D presence of CO presence of bicarbonate buffer addition of bases none of theseWP unacademy 22. How does COz help in the cell metabolism during cell culture? A. It participates in the de novo synthesis of purines and pyrimidines Helps in the cells respiration For monitoring pH of the culture All of the above‘Z unacademy 24. Cells which have undergone transformation frequently become A. B c. D. anchorage independent anchorage dependent stable unstable'Z unacademy 30. Which of the following is not a source of energy in active muscle cells? A. Creatine phosphate B. ATP C. Lactic acid D Glucose