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  • 5 - Stool - Fresh 1 PDF

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    Ahmed Gaber
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    The document provides instructions for performing a fresh stool examination under a microscope. It outlines the necessary materials, including microscope slides, cover slips, saline solution, and a wooden stick. It describes the steps to prepare a wet mount, which involves placing a drop of saline on a slide, mixing a small amount of stool with the saline, avoiding air bubbles when applying a cover slip, and examining the sample under different microscope objectives to identify helminth ova or amoebae.

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    The document provides instructions for performing a fresh stool examination under a microscope. It outlines the necessary materials, including microscope slides, cover slips, saline solution, and a wooden stick. It describes the steps to prepare a wet mount, which involves placing a drop of saline on a slide, mixing a small amount of stool with the saline, avoiding air bubbles when applying a cover slip, and examining the sample under different microscope objectives to identify helminth ova or amoebae.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    28 views12 pages

    5 - Stool - Fresh 1 PDF

    Uploaded by

    Ahmed Gaber
    The document provides instructions for performing a fresh stool examination under a microscope. It outlines the necessary materials, including microscope slides, cover slips, saline solution, and a wooden stick. It describes the steps to prepare a wet mount, which involves placing a drop of saline on a slide, mixing a small amount of stool with the saline, avoiding air bubbles when applying a cover slip, and examining the sample under different microscope objectives to identify helminth ova or amoebae.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 12

    Methods in Parasitology

    5. Fresh Stool Examination


    (Wet mount)

    Swiss Tropical Institute, Basel

    April 2005
    5.0 Fresh Stool Examination

    • Contents
    • 5.1 Materials
    • 5.2 Preparing wet mount
    • 5.7 Examining wet mount

    Production Team:
    Yvette Endriss, Elisabeth Escher & Birgit Rohr
    Prof. Hanspeter Rohr (NeoCortex Foundation)
    Prof. Niklaus Weiss (STI)
    5.1 Fresh Stool Examination

    Materials:

    • Microscope slides
    • Cover slips
    • Sodium chloride
    solution in small
    bottle with pipette
    • Wooden stick
    • Fresh stool
    • Gloves
    5.2 Fresh Stool Examination

    Use cleaned microscope


    slides
    5.3 Fresh Stool Examination

    Place a drop of saline


    5.4 Fresh Stool Examination

    Take a small amount


    of stool with a wooden
    stick
    5.5 Fresh Stool Examination

    Mix stool with saline

    Clip
    5.6 Fresh Stool Examination

    Mistake:
    Too much stool!
    5.7 Fresh Stool Examination

    Place coverslip

    Avoid air bubbles!


    5.8 Fresh Stool Examination

    Examination of helminth
    ova :
    Use 10x objective

    Photo:
    Schistosoma mansoni
    5.9 Fresh Stool Examination

    Examination of
    amoebae:

    Press cover slip slightly,


    remove excess liquid
    with paper towel

    Clip
    5.10 Fresh Stool Examination

    For amoebae:

    Use 50x objective with


    oil immersion

    Example:
    Entamoeba coli, cyst

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