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  • Gel Number6 Percentage5 13.50 Volume 24 30% A/B4.00 6.00 Volume (ML/) Conc Entra Tion 4ml

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    Ren Yi
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    This document provides instructions for preparing two different polyacrylamide gel solutions, including specifying the volumes and concentrations of water, acrylamide/bis solution, buffer solution, SDS, APS, and TEMED to use for each gel. The first gel is a 30% acrylamide solution and the second is a 12% acrylamide solution.

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    WideRange

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    This document provides instructions for preparing two different polyacrylamide gel solutions, including specifying the volumes and concentrations of water, acrylamide/bis solution, buffer solution, SDS, APS, and TEMED to use for each gel. The first gel is a 30% acrylamide solution and the second is a 12% acrylamide solution.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
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    5 views1 page

    Gel Number6 Percentage5 13.50 Volume 24 30% A/B4.00 6.00 Volume (ML/) Conc Entra Tion 4ml

    Uploaded by

    Ren Yi
    This document provides instructions for preparing two different polyacrylamide gel solutions, including specifying the volumes and concentrations of water, acrylamide/bis solution, buffer solution, SDS, APS, and TEMED to use for each gel. The first gel is a 30% acrylamide solution and the second is a 12% acrylamide solution.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    Gel Number6 Volume (mL/uL)

    Conc
    Percentage5 H2O 13.50
    entra
    tion Volume 24 30% A/B4.00
    4ml/
    Wide Range Buffer
    6.00
    10% SDS (uL)
    240
    10% APS (uL)
    240
    TEMED (uL)
    23.0
    Conc
    Percentage12 H2O 7.91
    entra
    tion Volume 24 30% A/B9.60
    4ml/
    Wide Range Buffer
    6.00
    10% SDS (uL)
    240
    10% APS (uL)
    240
    TEMED (uL)
    9.6

    2.2 HO
    ml/gVolume13.2 2 7.86
    el Percen 340% A/B1.76
    Wide Range Buffer
    3.30
    10% SDS (uL)
    132
    10% APS (uL)
    132
    TEMED (uL)
    12.7

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