C190-E092B

Prominence Gel Permeation Chromatography System

Prominence GPC System

Prominence GPC System
Prominence Gel Permeation Chromatography System
Highly Reliable Analytical Results in Every Field
The Prominence GPC System provides highly reliable and highly extensible performance in a wide variety

of applications. Hardware that provides rapid instrument stabilization and excellent reproducibility of

analytical results, and software that includes analytical workflow automation and overlap injection

functions and features a user-friendly analysis screen view contribute to improving productivity.
Shimadzu has worked hard on every individual element influencing the basic performance of the

Prominence GPC System to provide highly reliable analytical results for all customers.
 Assembling Elements Demanded in GPC Analysis
Excellent Reproducibility in Molecular Weight Distribution Analysis
For the analysis of molecular weight distribution, since the
molecular weight of compounds eluted between the exclusion
limit and permeation limit are considered an exponential function
of elution time (calibration curve), even a small variation in
elution time causes a substantial change in the result.
Excellent Stability Maintained Even During Subtle Changes in
Room Temperature
Because of a temperature control function to suppress the
effects of room temperature fluctuations, Prominence
detectors achieve excellent baseline stability and
reproducibility. Generally speaking, differential refractive
µ °C
Room temperature
Room Temperature
Baseline
RID-20A Without Cell-Temperature Control
4
The Prominence GPC System utilizes high-speed micro-plunger
actuation and automatic pulse compensation to provide
pulse-free solvent delivery and excellent elution time
reproducibility.
The analysis of polystyrene (N=20 / day),
interval difference in a day was very small.
index detectors tend to be easily affected by changes in room
temperature. The optical system of the RID-20A, however, has
a dual temperature control function that absorbs the effects of
changes in room temperature, which ensures excellent stability.
µ °C
Room Temperature
Room temperature
Baseline
RID-20A Cell Temperature Controlled at 40°C
The RID -20A Differential Refrac tive Index Detec tor Allows
Productivity Improvements in GPC Analysis
Inheriting the stability and extensibility that are the strengths
of the Prominence series, the new RID-20A model of
differential refractive index detector is designed with a new
reference-cell auto-purge feature and validation support
function that dramatically improve GPC analysis productivity.

Reduced Stabilization Time and Improved Baseline Stability:

Af ter 30 Minutes Power on
µRIU
The RID-20A achieves shorter baseline stabilization time after
turning ON the power through improved dual-temperature
control of the optical system and superior lamp performance.
The stable baseline ensures reliable molecular weight
distribution analysis.

min

Elapsed time after power ON

30%* Savings in Solvent Usage over Conventional Performance and

Reduced Environmental Burden
The amount of mobile phase consumed can be saved by returning column eluate to the mobile phase bottle during intervals
when no component peaks are eluted. Cost of analysis per sample is reduced and burden placed on the environment is mitigated.
The recycle valve kit can be attached to the RID-20A and the SPD-20A/20AV.
µRIU
When performing a 15-minute analysis 50 times (flow rate: 1 mL/min)

Without recycle valve kit ... 750 mL

With recycle valve kit ... 500 mL Normal

→ Mobile phase usage reduced by approx. 30% * (* under sp e cifie d conditions )

Recycle
The use of the recycling valve
cannot affect the baseline and
the peak shape.

Shimadzu's Proprietar y Technology Suppor ts Highly Sensitive

Analysis to Preparative Analysis Applications
The four-partition photodetector in the RID-20A allows a wide refractive index range (0.01 to 5000 µRIU). The single detector supports
all applications from highly sensitive measurements to preparative measurements using the three operation modes shown below.
µRIU

A (Analytical) Mode High-sensitivity to general-purpose analysis

High-concentration analysis, semi-preparative

P (Preparative) Mode
analysis (up to 20 mL/min) A mode P mode

Flow selection block allows large-volume

L (Large-scale prep.) Mode
preparative analysis. (up to 150 mL/min)

mg/L

Conc. of sucrose

Prominence GPC System

Prominence Gel Permeation Chromatography System 5
 Supports Work Optimization

Automation of Workflow
Numerous functions supporting the analysis process sequence, from
Instrument
instrument startup to shutdown, significantly improve procedural Power ON
efficiency. In addition, baseline drift checks can be performed
automatically, eliminating the need to wait by the instrument for Startup
detector stabilization.
Start of solvent delivery
and temperature control
at the specified time

Automated and Easy System Suitability Auto-purge

Testing ( SST)
Purging of flow lines via
the mobile phase for
The LabSolutions software is designed with a function to use a control analysis

sample or standard sample to check system compatibility prior to

Baseline check
measurement of an unknown sample.
This function can be included in batch analysis to allow the actual Baseline stabilization
(noise and drift check)
sample to be measured only when a positive test result is obtained. The
software can also output test results in text format or CSV format.
Analysis

From start to finish of

the analysis

Report
generation

Shutdown

From stopping of solvent

delivery and temperature
control to shutdown

Shor t analysis time — Overlap Injection Function

Overlapping injections combined with the SIL-30AC Nexera X2 autosampler can significantly save analytical
time for multiple cycles and enable higher throughput.

μRIU Normal Injection

10 injection cycles of a 15-minute analysis
2
Normal Injection cycles … 2 h 30 min 1
With Overlapping Injection … 1 h 40 min 0
→ Save approximately 35% on total analysis time -1

0 5 10 15 20 25 30 35 40 min
1st inj. 2nd inj. 3rd inj.

μRIU Overlapping Injection

2
1
0
-1

0 5 10 15 20 25 30 min
1st inj.
2nd inj.
3rd inj.

As with normal analysis, molecular weight can be calculated from the analysis window.

6
Suppor ts Smar t Data Management
Using LabSolutions for integrated management of data from
various locations ensures analytical reliability and improved
efficiency of analysis. Control and analysis using GC and HPLC
systems, including the GPC system, are also possible from a
single graphical interface.
LabSolutions includes a wide range of functions that meet the
requirements of guidelines such as GLP/GMP and FDA 21 CFR
Part 11, and provides secure data management.

〈N e t wo r k C o m p a ti b ili t y : L a b S o l u ti o n s C S〉

Client PC *2
Laboratory or office iPad *3, 4 LabSolutions server

Acquisition controller PC *1
*1 Acquisition controller PC

Shimadzu LC Shimadzu GC Shimadzu LC Shimadzu GC Shimadzu FTIR Agilent LC *5 Agilent LC *5

Thermal Particle Size

UV FTIR TOC Balances Analysis Distribution AA

Integrated management of the data of these instruments is also possible through combination with CLASS-Agent.

*1
The acquisition controller PC controls the analytical instruments.
Analysis directions and re-analysis of data can be performed using a client PC.
*2
It is not necessary to install LabSolutions software on the client PC for terminal service.
*3
iPad is a registered trademark of Apple Inc.
*4
When using an iPad, the installation of Citrix’s XenApp is required.
*5
Agilent's LC instruments can be controlled. Agilent's GC instruments will be supported in the future.

Suppor ts Whole-System Instrument Management

The Prominence GPC System checks a history of maintenance to identify column names and serial numbers and to create a
and operation for each module, which enables quick and easy usage history that includes the amount of mobile phase that
inspection and maintenance of the instrument. has passed through the column and number of sample
An optional column management device also makes it possible injections.

Prominence GPC System

Prominence Gel Permeation Chromatography System 7
 LabSolutions GPC Software
Easy Analysis of Molecular Weight Distribution via Graphical Interface

Create a Calibration Cur ve in Just Three Steps

Drag and drop standard-sample data into the window.

Load the retention times.
Enter the molecular weight of the standard sample.

A maximum of 64 data points are available. Virtual points are also easy to set, and calibration curve appropriateness can be
checked visually while choosing from a wide variety of approximation equations. Calibration curves can be corrected using the
Mark-Houwink equation, and other correction methods based on Q-factors or degree of polymerization are available.

Graphical GPC Data Analysis Window

・Manipulation of peak integration possible by means of the

graphical interface
・Management of data from multiple detectors within a single file

Because the molecular weight distribution curve is updated

whenever peak integration is performed, results for mean
molecular weight, intrinsic viscosity, polydispersity, and other
parameters can be confirmed immediately.
Time and detector sensitivity can also be corrected based on an
internal standard peak or control sample.

The Data Comparison Window Allows Simultaneous Evaluation of

Multiple Samples

・The elution curves and derivative and integrated molecular

weight distribution curves for up to 10 samples can be
overlaid on a graph.
・Statistical results can be displayed for mean molecular
weight, intrinsic viscosity, and polydispersity.

8
 1/31/2014 2:42:53 P M 1 / 1

Diverse Range of Repor t Formats Sample

Vial #
==== S him adzu LabS olutions G P C A nalysis R eport ====
Analyst

Sample ID
Inj. Vol.
Data File
Method File
:
:
:
:
:
:
:
Admin
GPC Demo
UNK
5
50 uL
GPC_Data-005.lcd
GPC_Method.lcm
Batch File : GPC_Demo_Batch.lcb
Report File : GPC_Demo(DetA).lsr
Acquisition Date : 2005/04/07 1:38:47
Modified Date : 2014/01/20 16:18:21

Calibration Curve Molecular Weight Distribution

LabSolutions is equipped with report templates for a variety of

uRIU log(M.W.) %
7

14.974
100

Mw=646690
0.75
6
75

Mz=1292398
0.50
5

analysis results. The software can accommodate a diverse

50

Mn=96308
0.25
25

Mz1=1893795
Mz1=1
3
0.00 0

range of reports thanks to a wealth of report items and a 12.5

P eak R eport
RID Channel 1
Peak# Ret. Time
15.0

Area
17.5

Height
20.0
min

Area% Height%
4 5 6
log(M.W.)
log(M W )
12/27/2013 6:43:11 P M 1 / 1

1 14.974 145667 848 100.000 100.000

Total 145667 848 100.000 100.000
==== S him adzu LabSolutions G P C Sum m ary R eport====
highly flexible layout. G P C C alculation R esult
Peak#:1 (RID Channel 1)
[Peak Information]
Title Time (min) tion Volume (lecular Weig
Start 12.133 12.133 6078726
Height
9
C hrom atogram
%

100
Top 14.974 14.974 692160 848
End 19.917 19.917 1411 50

In addition, a PDF output function is included as standard, so Area : 145667

Area% : 100.0000

[Average Molecular Weight]

Number Average Molecular Weight (Mn)
Weight Average Molecular Weight (Mw)
96308
646690
75

50
Z Average Molecular Weight (Mz) 1292398
Z+1 Average Molecular Weight (Mz1) 1893795
Mw/Mn 6.71480 25

analysis results reports can be managed by automatic import

Mz/Mw 1.99848
1
2
0 3
4
14 15 16 17 18 19 20
m in

to a database, helping your laboratory to go paperless, and

M olecular W eight D istribution C um ulative P ercent
% %
100 100

75 75

promoting an eco-friendly analysis process. 50

25
50

25
1 1
2 2
0 3 0 3
4 4
4 5 6 4 5 6
log(M .W .) log(M .W .)
C hrom atogram D etector 1 C h1
# File nam e Mn Mw Mz M z1 M w /M n M z/M w
1 G P C _D ata-001.lcd 10270 53317 112024 136034 5.19152 2.10109
2 G P C _D ata-002.lcd 20561 106621 222988 270061 5.18551 2.09141
3 G P C _D ata-003.lcd 38998 209917 442293 525799 5.38270 2.10699
4 G P C _D ata-004.lcd 80814 429500 907559 1084610 5.31469 2.11306
A verage 37661 199839 421216 504126 5.26860 2.10314
%R S D 82.650 83.236 83.585 83.195 1.833 0.438
M axim um 80814 429500 907559 1084610 5.38270 2.11306
M inim um 10270 53317 112024 136034 5.18551 2.09141
SD 31127 166337 352074 419408 0.09659 0.00922

Reduce the Work Involved in Creating a Final Repor

por t
Do you move your analytical results to a spreadsheet program (e.g., Excel) to create a final report?
LabSolutions includes a multi-data report feature, which reduces the work involved in report creation. Analytical results are
automatically entered into a spreadsheet equivalent to the one used in Excel, eliminating the need to move the data.

〈Conventional Workflow for Repor t Creation〉

*3&6XPPDU\5HSRUW
$QDO\VW 6\VWHP$GPLQLVWUDWRU ,QVWUXPHQW *3&6\VWHP
6DPSOH 3RO\VW\UHQH 0HWKRG)LOH 0HWKRGOFP
,QM9RO ̭/ %DWFK)LOH %DWFKOFE
$FTXLVLWLRQ'DWH  0RGLILHG'DWH

==== S him adzu LabS olution㼟㻌G P C A nalysis R eport ====

R ID C hrom atogram Calibration plot
uRIU
6
12.152

&DOLEUDWLRQ7DEOH
5.5
57PLQ 0: /RJ0:
   5
  
min

log (M.W.)
   4.5
G P C C alculation R esult
% G P C C alculation R esult   
P eak#:1 (㻾㻵㻰 C h1)㻌
[平均分子量]    4
Mw=5603489

N um ber A verage M olecular W eight (M n) 4648548

Mz=6390432

W eight A verage M olecular W eight (M w ) 5603489

  
Mz1=7056187

Z A verage M olecular W eight (M z) 6390432

Mn=4648548

Z+1 A verage M olecular W eight (M z1) 7056187

M w /M n 1.20543    3.5
M z/M w 1.14044
Intrinsic V iscosity 1.00000
% 100.0000
3

log(M.W.)
2.5
㻜 㻡 㻝㻜 (min)
P D A S pectrum and Q uantitative R esult
ID # :1
RT :16.210
N am e :Irganox

m AU $YHUDJH0ROHFXODU:HLJKW
2000 194.56  )LOHQDPH 57
0Q 0Z 0] 0] 0Z0Q 0]0Z
1500
Q uantitati㼢㼑 R esult  6DPSOHBRYHUODSB       
PD A
1000 ID # N am e  6DPSOHBRYHUODSB       
1 Irganox

500 RT A rea  6DPSOHBRYHUODSB       
16.210 460032
252.71 276.87
 6DPSOHBRYHUODSB       
0 H eight C onc.
 6DPSOHBRYHUODSB       
21445 0.791
190 200 210 220 230 240 250 260 270 280 290
nm
 6DPSOHBRYHUODSB       
 6DPSOHBRYHUODSB       

C :㼈LabS olutions㼈S am ple㼈LC 㼈m ix2_pro9-50-042_2x2_add5_2.lcd

 6DPSOHBRYHUODSB       
$YHUDJH       
56'       

Analysis and data acquisition

0D[       
0LQ       

・Time and effort associated with transfer tasks, entry errors, and double checking
Transfer of results reports to
・Problems of paper/file management
a spreadsheet program
・Time and effort associated with regular verification work

〈Workflow of Repor t Creation with the Multi- Data Repor t〉

Printing or
conversion to
PDF format

Analysis and data acquisition Automatic report Automatically saved

→
creation to the database

・Elimination of transfer errors

Transfer of results is ・Easy management of reports via the database
unnecessary
・Appropriate file protection provided by an audit trail feature

Note: Excel is a registered trademark of Microsoft Corporation.

Prominence GPC System

Prominence Gel Permeation Chromatography System 9
 Superior Extensibility of the Prominence Series

Choose a Detec tor Designed to Your Objec tives

A lineup of detectors is available, comprising the RID-20A analysis of industrial polymers to water-based GPC applications
differential refractive index detector, photodiode array for analysis of biopolymers. Also, using AccuSpot allows you to
detectors, UV-VIS detectors, and evaporative light-scattering construct an automated analysis system capable of easy online
detectors. Select from a variety of detectors according to processing—everything from GPC separation to MALDI-TOF MS
application, from organic solvent-based GPC applications for measurement.

Refractive Index Detector

RID-20A

Photodiode Array Detector

SPD-M20A Evaporative
Light-Scattering Detector
ELSD-LT II

UV-VIS Detector
SPD-20A MALDI-TOF MS
AXIMA Series

Perform Diverse Analyses Within a Single Measurement

Using a differential refractive index detector together with a to measure molecular weight distribution, but also to identify
photodiode array detector enables high-sensitivity detection of and quantitate additive agents and impurities contained in
peaks that are indistinct when analyzed with the differential polymers, all in a single analysis.
refractive index detector alone. This makes it possible not only

Polystyrene include additives : RID

Polystyrene include additives : PDA

Additives (Inganox 1010) : PDA

Measurement of molecular Quantitation of additive agents

weight distribution
Concentration
%
Molecular weight distribution curve 5
100
Mw=5603489
Mz=6390432

4
80
Mz1=7056187
Mn=4648548

60 3

40 2

20 1

0
0
5.8 5.9 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 7.0 log (M.W.) 0 50000 100000 150000 200000 250000 Area

10
Shim-pack GPC Series Columns for GPC Measurement
Because each Shim-pack GPC series column is packed with polystyrene-based polymers with different degrees of crosslinking, the
optimal column can be chosen according to intended use with anything from polymers to oligomers.

Shim-pack GPC- 80 0 Series High- Per formance GPC Columns

For use with tetrahydrofuran (800 Series)
P/N Product Name Exclusion Limit Molecular Weight (polystyrene) Size (length × inner diameter, mm)
228-20803-91 Shim-pack GPC-801 1.5 × 103 300 × 8.0
228-20804-91 Shim-pack GPC-802 5 × 103 300 × 8.0
228-20805-91 Shim-pack GPC-8025 2 × 104 300 × 8.0
228-20806-91 Shim-pack GPC-803 7 × 104 300 × 8.0
228-20807-91 Shim-pack GPC-804 4 × 105 300 × 8.0
228-20808-91 Shim-pack GPC-805 4 × 106 300 × 8.0
228-20809-91 Shim-pack GPC-806 4 × 107 300 × 8.0
228-20810-91 Shim-pack GPC-80M 4 × 107 Mixed gel 300 × 8.0
228-20811-91 Shim-pack GPC-807 2 × 108 300 × 8.0

For use with chloroform (800C Series)

P/N Product Name Exclusion Limit Molecular Weight (polystyrene) Size (length × inner diameter, mm)
228-20803-92 Shim-pack GPC-801C 1.5 × 103 300 × 8.0
228-20804-92 Shim-pack GPC-802C 5 × 103 300 × 8.0
228-20805-92 Shim-pack GPC-8025C 2 × 104 300 × 8.0
228-20806-92 Shim-pack GPC-803C 7 × 104 300 × 8.0
228-20807-92 Shim-pack GPC-804C 4 × 105 300 × 8.0
228-20808-92 Shim-pack GPC-805C 4 × 106 300 × 8.0
228-20809-92 Shim-pack GPC-806C 4 × 107 300 × 8.0
228-20810-92 Shim-pack GPC-80MC 4 × 107 Mixed gel 300 × 8.0
228-20811-92 Shim-pack GPC-807C 2 × 108 300 × 8.0

For use with dimethylformamide (800D Series)

P/N Product Name Exclusion Limit Molecular Weight (polystyrene) Size (length × inner diameter, mm)
228-20803-93 Shim-pack GPC-801D 1.5 × 103 300 × 8.0
228-20804-93 Shim-pack GPC-802D 5 × 103 300 × 8.0
228-20805-93 Shim-pack GPC-8025D 2 × 104 300 × 8.0
228-20806-93 Shim-pack GPC-803D 7 × 104 300 × 8.0
228-20807-93 Shim-pack GPC-804D 4 × 105 300 × 8.0
228-20808-93 Shim-pack GPC-805D 4 × 106 300 × 8.0
228-20809-93 Shim-pack GPC-806D 4 × 107 300 × 8.0
228-20810-93 Shim-pack GPC-80MD 4 × 107 Mixed gel 300 × 8.0
228-20811-93 Shim-pack GPC-807D 2 × 108 300 × 8.0

GPC- 80M Mixed Gel Columns ( 80MC , 80MD)

Mixed gel columns that produce linear calibration curves over a wide range of
molecular weights.

Shim-pack GPC-20 0 0 Series Preparative Columns

Preparative columns for use with chloroform and tetrahydrofuran.
These columns have substantial sample processing power as well as separation
power comparable to that of columns for analytical use.

Prominence GPC System

Prominence Gel Permeation Chromatography System 11
 Prominence GPC System
Prominence GPC System Standard Configuration
P/N Description Model Qty
228-45012-XX System Controller CBM-20A*1 1
228-45000-XX Solvent Delivery Unit LC-20AD*2 1
228-45018-XX Degassing Unit DGU-20A3R*2 1
228-45041-91 Reservoir Tray 1
228-45119-XX Autosampler SIL-20AHT *2 1
228-48258-91 Vespel Needle Seal 1
228-15652-92 Vial 1.5 mL 1
228-45009-XX Column Oven CTO-20A 1
228-45104-XX Refractive Index Detector RID-20A 1
— Analysis column Shim-pack GPC Series*3 —
— LC Workstation LabSolutions*4 1
223-07738-92 GPC Software LabSolutions GPC Software*5 1

*1 : The CBM-20Alite (228-45011-XX) can also be chosen.

*2 : If the mobile phase includes HFIP, an HFIP-compatible degassing unit and HFIP-compatible kit are required.
*3 : Choose according to the range of molecular weights to be measured.
*4 : A printer and cable are required separately.
*5 : LabSolutions DB GPC software (223-13812-92) and LabSolutions CS GPC software (223-18995-92) can also be chosen.

Prominence GPC System Standard Specifications

Item Specifications Item Specifications
System configuration Modular Linear, 3rd-order, 3rd-order + hyperbolic
Calibration curve
curve, 5th-order, 5th-order + hyperbolic
Measurement method Single Flow approximation
curve, 7th-order, 7th-order + hyperbolic
formulae
Mobile phase delivery method Parallel Double Plunger curve, or broken line
Mobile phase flow rate setting range 0.0001 to 10.0000 mL/min Calibration curve Internal standard correction, Q-factor,
No more than 1% or 2 μL/min, correction RID sensitivity correction
Mobile phase flow rate accuracy functions
whichever is greater (0.01 to 2 mL/min)
No more than 0.06% RSD or 0.02 min SD, Automatic processing according to
Mobile phase flow rate precision Peak Integration
parameter settings. (Manipulation possible)
whichever is greater
Molecular weight Mn, Mw, Mz, Mz1, Mv, Mw/Mn, Mv/Mn, Mz/Mw,
Mobile phase degassing method Vacuum membrane
calculations and intrinsic viscosity
Degassing line flow rate 380 μL Data output ASCII format
Sample injection method Variable loop weighing
Sample injection volume setting range 0.1 to 100 μL (standard)
Number of samples processed 105 + 10 (1.5 mL vials)
Column temperature control method Forced Air Circulation
Column temperature control range (room temperature + 10°C) to 85°C
Detector noise 2.5 × 10−9 RIU max.
Detector drift 1 × 10−7 RIU/h max.
Detector cell volume 9 μL
Detector cell temperature control range 30 to 60°C

Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its
affiliates, whether or not they are used with trademark symbol “TM” or “®”.
Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu
disclaims any proprietary interest in trademarks and trade names other than its own.

For Research Use Only. Not for use in diagnostic procedures.

The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu
does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

www.shimadzu.com/an/ © Shimadzu Corporation, 2014

Printed in Japan 3655-04413-30ANS