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Presentation of Contents
1. Somatic gene therapy, which involves introducing a "good" gene into targeted cells
to treat the patient — but not the patient's future children because these genes do not get
passed along to offspring.
2. Germline gene therapy, involves the introduction of corrective genes into
reproductive cells (sperm and eggs) or zygotes, with the objective of creating a beneficial
genetic change that is transmitted to the offspring.
Appropriate methods to deliver DNA used in gene therapy are vital, as the
targeted tissues must properly receive the appropriate genes. Gene therapy can
be carried out using naked DNA delivered directly Vectors for Gene Delivery.
Adenoviruses - are a group of common viruses that infect the lining of your eyes, airways
and lungs, intestines, urinary tract, and nervous system. They're common causes of fever, coughs,
sore throats, diarrhea, and pink eye.
Source: www.andrew.cmu.edu
Retrovirus is a type of RNA virus that inserts a copy of its genome into the
DNA of a host cell that it invades, thus changing the genome of that cell.
Herpes simplex type 1 causes sores around the mouth and lips (sometimes called fever blisters
or cold sores). HSV-1 can cause genital herpes, but most cases of genital herpes are caused
by herpes type 2. In HSV-2, the infected person may have sores around the genitals or rectum.
The first death associated with gene therapy occurred on September 18, 1999,
at the University of Pennsylvania. Jesse Gelsinger was participating in a
clinical trial, a biomedical experiment for evaluation of safety and efficiency
of a therapy for a disease. Gelsinger, who was 18 years old at the time of the
treatment, had a deficiency of ornithine transcarboamylase, an important
enzyme in the metabolism of ammonia. Patients with this rare metabolic
disorder must maintain a low-protein diet and take a series of medicines to
avoid ammonia poisoning in the blood stream. The gene therapy Gelsinger
took triggered a chain reaction in his immune system, resulting in hepatic and
respiratory failure, and consequently, his death four days after being treated.
Effects on Human
Looking at the fact that genetic engineering employs viral vector that carries functional gene
inside the human body; the repercussion are still unknown. There are no clues as to where
functional genes are being placed. They may even replace the important genes, instead of
mutated genes. Thus, this may lead to another health condition or disease to human. Also, as
defective genes are replaced with functional gene, then it is expected that there will be a
reduction in genetic diversity and if human beings will have identical genomes, the population
as a whole will be susceptible to virus or any form of diseases.
Antibiotic Resistance
Genetic engineering often uses genes for antibiotic resistance as "selectable markers." Early in
the engineering process, these markers help identify cells that have taken up foreign genes.
Although they have no further use, the genes continue to be expressed in plant tissues. Most
genetically engineered plant foods carry fully functioning antibiotic-resistance genes.
The presence of antibiotic-resistance genes in foods could have lethal effects. Therefore,
eating these foods could reduce the effectiveness of antibiotics to fight disease when these
antibiotics are taken with meals. More so, the resistance genes could be transferred to human
or animal pathogens, making them impervious to antibiotics. If transfer were to occur, it could
aggravate the already serious health problem of antibiotic resistant disease organisms