Obtain 4pcs Smith

fermentation tubes. Pour

enough volume to fill one-
third of the fermentation
tubes with the following
prepared solutions to the
respective tubes: Tube 1:
dH2O Tube 2: glucose
Tube 3: lactose Tube 4:
Compute for starch
the volume of
the gas evolved
and the rate of
CO2 evolution
by V= πr^2h.
Add one-third
volume of distilled
water and another
one-third volume of
yeast suspension to
each tube. Make
sure no bubbles are
trapped at the close
end.

Compute for
the mean
measurements
per tube as well Cover the openings
as standard with saran wrap or
deviation. parafilm. Tie the
tubes together at
their vertical arms
Incubate the tubes to keep it upright
at 37 degree using a rubberband.
Celsius. Measure
the height of the
area occupied by
the CO2 evolved
every 10 min. for
40 min.