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Bioenergetics: Theory and Application To Fitness and Performance
Bioenergetics: Theory and Application To Fitness and Performance
Howley
Chapter
Bioenergetics
Objectives
Objectives
Outline
Introduction
• Metabolism
– Sum of all chemical reactions that occur in the body
– Anabolic reactions
Synthesis of molecules
– Catabolic reactions
Breakdown of molecules
• Bioenergetics
– Converting foodstuffs (fats, proteins, carbohydrates)
into energy
Cell Structure
• Cell membrane
– Semipermeable membrane that separates the cell
from the extracellular environment
• Nucleus
– Contains genes that regulate protein synthesis
Molecular biology
• Cytoplasm
– Fluid portion of cell
– Contains organelles
Mitochondria
Figure 3.1
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Chapter 3 Cell Structure
In Summary
Figure 3.2
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Chapter 3 Biological Energy Transformation
• Endergonic reactions
– Require energy to be added
– Endothermic
• Exergonic reactions
– Release energy
– Exothermic
• Coupled reactions
– Liberation of energy in an exergonic reaction drives
an endergonic reaction
Figure 3.3
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Chapter 3 Biological Energy Transformation
Coupled Reactions
The energy given off by the exergonic reaction
powers the endergonic reaction
Figure 3.4
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Chapter 3 Biological Energy Transformation
Oxidation-Reduction Reactions
• Oxidation
– Removing an electron
• Reduction
– Addition of an electron
• Oxidation and reduction are always coupled
reactions
• Often involves the transfer of hydrogen atoms
rather than free electrons
– Hydrogen atom contains one electron
– A molecule that loses a hydrogen also loses an
electron and therefore is oxidized
• Importance of NAD and FAD
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Chapter 3 Biological Energy Transformation
Figure 3.5
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Chapter 3 Biological Energy Transformation
Enzymes
Figure 3.6
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Chapter 3 Biological Energy Transformation
a) Substrate (sucrose)
approaches the
active site on the
enzyme.
b) Substrate fits into
the active site,
forming enzyme-
substrate complex.
c) The enzyme
releases the
products (glucose
and fructose).
Figure 3.7
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Chapter 3 Biological Energy Transformation
Clinical Applications 3.1
Diagnostic Value of Measuring Enzyme
Activity in the Blood
• Damaged cells release enzymes into the blood
– Enzyme levels in blood indicate disease or tissue
damage
• Diagnostic application
– Elevated lactate dehydogenase or creatine kinase in
the blood may indicate a myocardial infarction
Classification of Enzymes
• Oxidoreductases
– Catalyze oxidation-reduction reactions
• Transferases
– Transfer elements of one molecule to another
• Hydrolases
– Cleave bonds by adding water
• Lyases
– Groups of elements are removed to form a double bond or
added to a double bond
• Isomerases
– Rearrangement of the structure of molecules
• Ligases
– Catalyze bond formation between substrate molecules
• Temperature
– Small rise in body temperature increases enzyme
activity
– Exercise results in increased body temperature
• pH
– Changes in pH reduces enzyme activity
– Lactic acid produced during exercise
Figure 3.8
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Chapter 3 Biological Energy Transformation
Figure 3.9
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Chapter 3 Fuels for Exercise
Carbohydrates
• Glucose
– Blood sugar
• Glycogen
– Storage form of glucose in liver and muscle
Synthesized by enzyme glycogen synthase
– Glycogenolysis
Breakdown of glycogen to glucose
Fats
• Fatty acids
– Primary type of fat used by the muscle
– Triglycerides
Storage form of fat in muscle and adipose tissue
Breaks down into glycerol and fatty acids
• Phospholipids
– Not used as an energy source
• Steroids
– Derived from cholesterol
– Needed to synthesize sex hormones
Protein
In Summary
High-Energy Phosphates
ADP + Pi → ATP
• Breakdown
ATP ATPase
ADP + Pi + Energy
Structure of ATP
Figure 3.10
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Chapter 3 High-Energy Phosphates
Figure 3.11
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Chapter 3 Bioenergetics
Bioenergetics
• Formation of ATP
– Phosphocreatine (PC) breakdown
– Degradation of glucose and glycogen
Glycolysis
– Oxidative formation of ATP
• Anaerobic pathways
– Do not involve O2
– PC breakdown and glycolysis
• Aerobic pathways
– Require O2
– Oxidative phosphorylation
• ATP-PC system
– Immediate source of ATP
PC + ADP ATP + C
Creatine kinase
• Glycolysis
– Glucose → 2 pyruvic acid or 2 lactic acid
– Energy investment phase
Requires 2 ATP
– Energy generation phase
Produces 4 ATP, 2 NADH, and 2 pyruvate or 2 lactate
Figure 3.12
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Chapter 3 Bioenergetics
Figure 3.13
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Chapter 3 Bioenergetics
Figure 3.14
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Chapter 3 Bioenergetics
Figure 3.15
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Chapter 3 Bioenergetics
Figure 3.15
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Chapter 3 Bioenergetics
The addition of two H+ to pyruvic acid forms NAD and lactic acid
Figure 3.16
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Chapter 3 Bioenergetics
In Summary
ATP ATPase
ADP + Pi + Energy
Formation of ATP without the use of O2 is termed
anaerobic metabolism. In contrast, the production of ATP
using O2 as the final electron acceptor is referred to as
aerobic metabolism.
In Summary
The Three
Stages
of Oxidative
Phosphorylation
Figure 3.17 Copyright ©2009 The McGraw-Hill Companies, Inc. All Rights Reserved.
Chapter 3 Bioenergetics
• Fats
– Triglycerides → glycerol and fatty acids
– Fatty acids → acetyl-CoA
Beta-oxidation
– Glycerol is not an important muscle fuel during
exercise
• Protein
– Broken down into amino acids
– Converted to glucose, pyruvic acid, acetyl-CoA, and
Krebs cycle intermediates
Figure 3.19
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Chapter 3 Bioenergetics
Figure 3.20
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Chapter 3 Bioenergetics
A Closer Look 3.4
Beta Oxidation is the Process of
Converting Fatty Acids to Acetyl-CoA
• Breakdown of triglycerides releases fatty acids
• Fatty acids must be converted to acetyl-CoA to be
used as a fuel
– Activated fatty acid (fatty acyl-CoA) into
mitochondrion
– Fatty acid “chopped” into 2 carbon fragments
forming acetyl-CoA
• Acetyl-CoA enters Krebs cycle and is used for
energy
Beta Oxidation
Figure 3.21
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Chapter 3 Bioenergetics
In Summary
Grand Total 32
In Summary
Control of Bioenergetics
• Rate-limiting enzymes
– An enzyme that regulates the rate of a metabolic
pathway
• Modulators of rate-limiting enzymes
– Levels of ATP and ADP+Pi
High levels of ATP inhibit ATP production
Low levels of ATP and high levels of ADP+Pi stimulate ATP
production
– Calcium may stimulate aerobic ATP production
Figure 3.22
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Chapter 3 Control of Bioenergetics
Glycolysis Phosphofructokinase AMP, ADP, Pi, ↑pH ATP, CP, citrate, ↓pH
++
Krebs cycle Isocitrate ADP, Ca , NAD ATP, NADH
dehydrogenase
In Summary
Figure 3.23
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Chapter 3 Interaction Between Aerobic/Anaerobic ATP Production
In Summary
Study Questions
Study Questions
Study Questions
Study Questions