CHAPTER 9 ■ REGULATION OF ENZYMES 151
kinetic parameters were then measured in the presence of ing this enzyme would provide the most protection to the
an allosteric activator. Which one of the following would
best describe the findings of that experiment?
A. A Vmax of 25 U/mg enzyme, and a Km,app of 0.2 mM.
B. A Vmax of 15 U/mg enzyme, with a Km,app of 2.0 mM.
C. A Vmax of 25 U/mg enzyme, with a Km,app of 2.0 mM.
D. A Vmax of 50 U/mg enzyme, with a Km,app of 5.0 mM.
E. A Vmax of 50 U/mg enzyme, with a Km,app of 10.0 mM.
5. A rate-limiting enzyme catalyzes the first step in the
conversion of a toxic metabolite to a urinary excretion
product. Which of the following mechanisms for regulat-
Lieberman_CH09.indd 151
body?
A. The product of the pathway should be an allosteric
inhibitor of the rate-limiting enzyme.
B. The product of the pathway should act through gene
transcription to decrease synthesis of the enzyme.
C. The toxin should act through gene transcription to
increase synthesis of the enzyme.
D. The enzyme should have a high Km for the toxin.
E. The toxin allosterically activates the last enzyme in
the pathway.
01/09/12 11:51 PM