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SYLLABUS LABORATORY ACTIVITY HISTOLOGY

GASTROINTESTINAL TRACT AND ASSOCIATED ORGAN
GASTROINTESTINAL SYSTEM

Resource Person : Maya Tejasari

Writter : Maya Tejasari
A Sequence
I. Introduction : 30 min
II. Pre Test :
III. Activity Lab : 120 min
- Discussion 30 min
- Identify 90 min
IV. Post Test :
B Topic
1. Principall layer of the Gastrointestinal Tract wall
2. Microstructure of oral cavity and pharynx
3. Microsturcuture of teeth and periodontal tissue
4. Microstructure of the oesophagus
5. Microstructure of the stomach
6. Microstructure of the small intestine
7. Microstructure of the large intestine
8. Microstructure of anorectal junction
9. Microstructure of the liver
10. Microstructure of the gall bladder
11. Microstructure of the pancreas
C Venue
Biomedical Laboratory Faculty of Medicine, Bandung Islamic Universtity

D Equipment
1. Light microscopy
2. Stained tissue section :

Oral cavity & pharynx Tongue, oropharynx

Teeth & periodontal

tissue

Section of 1/3 proximal, middle and

Oesophagus distal oesophagus

Stomach Section of fundus/body and pylorus

Small Intestine Section of duodenum, jejenum and

ilium
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Large intestine Section of colon, secum, appendyx

Anorectal junction Section of anorectal junction
Liver Section of liver
Gall bladder Section of gall bladder
Pancreas Section of pancreas
3. Colouring pencils

E Pre-requisite
Before following the laboratory activity, the students must prepare :

1. Draw and explain about principall wall of gastrointestinal tract wall

2. Draw the schematic picture of the tongue & oropharynx microstructure and give
explanation
3. Draw the schematic picture of the teeth and periodontl tissue microstructure and
give explanation
4. Draw the schematic picture of the oesophagus microstructure and give
explanation
5. Draw the schematic picture of the stomach (cardia,fundus,body,pylorus)
microstructure and give explanation
6. Draw the schematic picture of the small intestine (duodenum, jejenum,ilium)
microstructure and give explanation
7. Draw the schematic picture of the large intestine (colon, appendyx) microstructure
and give explanation
8. Draw the schematic picture of the anorectal junction microstructure and give
explanation
9. Draw and explain the schematic picture of the liver lobule microstructure and liver
lobule zone
10. Draw and explain the schematic picture of the gall bladder microstructure
11. Draw and explain the schematic picture of the pancreas microstructure

- Content lab in manual book ( pre and post test will be taken from the manual, if scorring
pre test less than 50, can not allowed thelab activity )
- Bring your text book, reference book e.q atlas of Histology, e-book etc. ( minimal 1
group 1 atlas ).
- Bring colouring pencils for drawing

F Activity Lab
1. Students will be divided into groups
2. Discussion in 30 minute
3. Identify tissue section using light microscopy and draw it , in 90 minute

4. LIST MICROSTRUCTURE IDENTIFY REVIEW ( give the checklist √ if you have already known)

Check list
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1. Section of tongue
- Identify layers and (mucous membrane, muscularis)
- Identify papilae and taste buds

DRAW

2. Section of teeth & periodontal tissue

- Identify enamel, dentin, pulp, cementum, periodontal ligament, alveolar bone,
Gum
DRAW
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3. section of oesophagus
- Low magnification :
* Identify layers of oesophageal wall (mucous membrane : epithelial
surface,lamina propia,muscularis mucosae, submucousa,
muscularis,serosa)

DRAW
Low magnification (40x, 100x)

High magnification (400x, 1000x)

Proximal, middle, distal oesophagus
*Identify High magnification : Identify the tissue of each layer (stratified
squomous epithelium,oesophageal glands, skeletal or smooth muscle
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4. section of Stomach (fundus/body)

- Low magnification :
* Identify layers of gastric wall (mucous membrane : epithelial
surface,lamina propia,gastric fundic glands (isthmus,neck,base),
muscularis mucosae, submucousa, muscularis (inner,middle,outer),
serosa)

High magnification
Identify layers of the wall and microstructure of each layer (simple columnar
epithelium,parietal/oxynthic cell, chief/zimogenic cell

5. Stomach (Pylorus)
High magnification
Identify layers of the wall and microstructure of each layer (simple columnar
epithelium, gastric glands

6. Duodenum
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- Low magnification :
* Identify layers of duodenal wall (mucous membrane : epithelial
surface,lamina propia :Lieberkhun glands, muscularis mucosae,
submucousa : Brunner gland, muscularis (inner,outer), serosa)

7. Jejenum
- Low magnification :
* Identify layers of jejenum wall (mucous membrane : epithelial
surface,lamina propia :Lieberkhun glands, muscularis mucosae,
submucousa, muscularis (inner,outer), serosa)

High magnification
Identify : Absortive cells (simple columnar epithelium with brush border)
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8. Ilium
- Low magnification :
* Identify layers of ilium wall (mucous membrane : epithelial
surface,lamina propia :Lieberkhun glands, muscularis mucosae,
submucousa : Brunner gland, muscularis (inner,outer), serosa)

9. Colon
- Low magnification :
* Identify layers of colon wall (mucous membrane : epithelial
surface,lamina propia, muscularis mucosae, submucousa, muscularis
(inner circular, outer longitudinal : taenia coli), serosa: appendices
epiploica)
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10. Appendyx
- Low magnification :
* Identify layers of appendyx wall (mucous membrane : epithelial
surface,lamina propia, muscularis mucosae, submucousa, muscularis
(inner,outer), serosa)

11. Anorectaal junction

- Low magnification :
* Identify : simple columnar epithelium, stratified squamous
epithelium, muscularis mucousae, plexus haemorrhoidaes, skeletal
muscle (levator ani)

12. Liver
Identify : central vein, portal triad (hepatic artery,portal vein,bile duct),
hepatocyte, kuffer cell, sinusoid)
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13. Gall bladder

Identify layers (mucosae, muscularis, perimuscular tissue, serosa)

12. Pancreas
- Low magnification : Identify endocrine (Islet of Langerhans) & ecxorine part
- High magnification : Identfy alpha & beta cell
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G Reference
1. Junqueria, Carneirro, Basic Histology

2. de'Fiore, Atlas of Histology

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