Table 2, Advantages and disadvantages of emerging methods for mycotoxin analysis Method Advantages Disadvantages LFD Rapid Semi-quantitative (visual assess- No clean up ment} No expensive equipment Cross-reactivity with related my- Easy to use cotoxins No specific training required Validation required for additional Quantitative for affatoxins matrices FPIA Rapid Inconsistent with ELISA or HPLC No clean up required analyses (except for DON) Validated for DON in wheat IR spectroscopy Rapid (NIR, MIR) Non-destructive measurement No extraction or clean up Easy operation ‘Capillary Rapid Electrophoresis Limited organic solvent use Good resolution of analyte from interfering substances Good sensitivity (fluorescence ca- pillary electrophoresis) Immunosensors! Rapid Biosensors. No clean up procedure (SPR, FOl, QCM, SPCE) MIP Low cost ‘Stable Reusable Poor sensitivity in some cases Cross-reactivity with related my- cotoxins Expensive equipment Calibration mode! must be validated Knowledge of statistical methods Poor sensitivity Expensive equipment Altemative to HPLC Clean-up may be required Extract clean-up needed to im- prove sensitivity Cross-reactivity with related my- cotoxins No labeling for detection Non-imprinted polymers may per- form similarly Poor selectivity