SEMESTER – 3

Biochemistry – Practical

1. To perform dialysis.
2. Protein estimation by any one: Lowry’s / Bradford’s method.
3. Separation between sugars and amino acids by thin layer chromatography.
4. To perform SDS – PAGE.
5. Calculation of void volume of sephadex G -25 column, using blue dextran.
6. Assay of any one enzyme under optimal condition.
7. To study the effect of temperature on the activity of enzyme.
8. To study the effect of pH on the activity of enzyme.

Human physiology and Anatomy-II – Practical

1. Simple reflex arc.

2. Physiological data acquisition based experiment (ECG).
3. Physiological data acquisition based experiment (EMG).
4. Physiological data acquisition based experiment (PFT).
5. To perform platelets count.
6. To determine the reticulocyte Count.
7. To perform tests for sensations (taste, torch, and smell).
8. Blood pressure recording in humans.
9. To study various types of contraceptive (condom, IUD’s, oral and injectable contraceptives).
10. To study different human organs and their sections through permanent slide

T.S. of thyroid, liver, thymus, spleen, ovary, artery, vein, capillary, testis, pancreas, oesophagus,
adrenal, kidney, urinary, bladder, urethra, fallopian tubes, epididymis, prostate glands, trachea,
bronchioles, pituitary, heart.