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11 Probe Calibration
11 Probe Calibration
2007-11
NOTE: This information is provided as an aid in Technical Support for trained support
personnel. Do not attempt to service the system without the appropriate technical training
and the proper tools.
Applicability All ADVIA Centaur® CP systems
Estimated Time 30 minutes
Special Notes This document provides instructions for calibrating the sample
probe and reagent probe to calibration points in the tip drawers,
the tip eject area, the sample compartment, ancillary reagent area,
primary reagent area, rinsing station, incubation ring, and system
reference point.
Action Perform these procedures before operating the system for the first
time. Repeat these procedures any time you replace or remove an
assembly for servicing.
Special Materials • Flashlight
• Metric ruler
Attachments Probes Calibration Checklist
References • Installation Guide (part number 086D0006-xx)
Contents
Introduction ....................................................................................................................... 3
Using the Probes Calibration Software .......................................................................... 4
Accessing the Software ................................................................................................ 4
Using the Probes Calibration window........................................................................... 5
Using the Keyboard ...................................................................................................... 6
Moving the Probes with the Cursor Keys ............................................................................6
Using the Shift Key for the Z-Axis ................................................................................ 7
Using the Calibration Window ...................................................................................... 8
Increment Bar......................................................................................................................8
X-, Y-, Z- Coordinates .........................................................................................................9
Previous Z ...........................................................................................................................9
Aligning the Probes to the Calibration Points ............................................................. 10
Calibration Points ....................................................................................................... 11
Calibrating the Sample Probe........................................................................................ 12
Calibrating the Probe to the System Reference Point................................................ 12
Calibrating the Probe to the Tip Drawers Coordinates............................................... 14
Calibrating the Probe to the Tip Eject Area Coordinates ........................................... 17
Calibrating the Probe to the Sample Compartment Coordinates ............................... 20
Calibrating the Probe to the Sample Probe Incubation Ring Coordinate................... 24
Calibrating the Reagent Probe ...................................................................................... 27
Calibrating the Probe to the System Reference Point................................................ 27
Calibrating the Probe to the Ancillary Reagent Area Coordinates ............................. 29
Calibrating the Probe to the Primary Reagent Area Coordinates .............................. 32
Calibrating the Probe to the Rinsing Station Coordinates.......................................... 34
Calibrating the Probe to the Reagent Probe Incubation Ring Coordinate ................. 39
Trademark Information................................................................................................... 40
Appendix.......................................................................................................................... 41
Probes Calibration Checklist ...................................................................................... 41
Introduction
This document provides instructions for calibrating the sample probe and reagent probe
to calibration points in the following areas:
• tip drawers
• tip eject area
• sample compartment
• ancillary reagent area
• primary reagent area
• rinsing station
• incubation ring
• system reference point
Perform these procedures before operating the system for the first time, as part of the
system installation (refer to the support bulletin Installation Guide, part number
086D0006-xx). Repeat these procedures any time an assembly containing calibration
coordinates or a probe is replaced or removed for servicing.
CAUTION
Ensure that you always use the smallest step increment for making a final calibration.
Using a larger step increment could result in damage to the probe.
CAUTION
Do not attempt to re-calibrate a probe if the probe crashes (goes offline) during the
calibration procedure. This could result in damage to the probe. If the probe crashes,
select Cancel to exit the window. Move the probes to a safe position. Start the program
and continue from the point at which the software crashed. If the program does not
respond, restart the computer.
When calibrating a probe to a z-axis coordinate, use the Shift key to move the probe close
to the surface in five half-increment steps (to 0.5, 0.25, 0.125, 0.0625, and 0.0312).
Probe
Starting Point
0.5
0.25
0.125
0.0625 0.0312
Calibration Pin
Then use the smallest increment step to move the probe down until it touches the surface
of the calibration pin.
This is the safest method of moving the probe.
CAUTION
Ensure that you use the lowest step increment for making a final calibration. Using a
larger step increment could result in damage to the probe. When making your final
adjustment to a calibration, always use the smallest step increment.
Use the increment bar to adjust the distance the probe moves per step increment. To
increase the step increment, move the increment bar up. To decrease the step increment,
move the increment bar down. In the lowest position, the step increment is minimal.
CAUTION
Ensure that you use the lowest step increment for making a final calibration. Using a
larger step increment could result in damage to the probe. When making your final
adjustment to a calibration, always use the smallest step increment.
Back
y
Left
x x
y Right
z
Front Down
The number of steps translates to the distance according to the following table:
Axis Direction Number of Steps Distance
x left-right 10 1 mm
y front-back 8 1 mm
z up-down 20 1 mm
Previous Z
CAUTION
Do not select the Previous Z button unless you are familiar with the previous calibrated
position. Using the Previous Z feature without knowing its actual previous position could
result in damage to the probe.
The Previous Z button allows you to move the probe down to the last calibrated z-axis
coordinate.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
4. To save the coordinates, select OK.
The probe moves to the next position.
Calibration Points
When calibrating the probes to the calibration points, follow the numeric order indicated
below. Always calibrate the sample probe to the system reference point (calibration point
8) before calibrating points 1 – 10. Always calibrate the reagent probe to the system
reference point (calibration point 8) before calibrating points 11 – 19.
CAUTION
Do not attempt to re-calibrate a probe if the probe crashes (goes offline) during the
calibration procedure. This could result in damage to the probe. If the probe crashes,
select Cancel to exit the window.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
3. Calibrate the sample probe to the system reference point (calibration point 8):
a. Using the keyboard controls, align the probe to the calibration pin.
CAUTION
Do not select the OK button unless the calibration is complete for that coordinate.
Selecting OK prior to completing the procedure overwrites the previously calibrated
coordinates and could damage the probe. If you need to stop the calibration before
completing the procedure, stand clear of the probes, and select Cancel.
b. Select OK.
The probe moves to the home position.
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
Figure 11. Tip Eject Area (calibration point 5, back eject position)
d. Select OK.
The probe moves to the next position.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
CAUTION
Ensure that the tip adapter sleeve does not touch the housing when the sample probe
ejects a tip. If it does, the tip will not drop correctly. The distance between the tip adapter
and the metal housing must be greater than 1 cm.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
1. Ensure that a tip is present in the left-back position of the right-back tip tray (see
below).
This tip is used for determining the z-coordinate during this calibration procedure.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
1. Remove the plastic incubation ring cover:
a. Using a Phillips screwdriver, loosen the captive screw at the front of the
incubation ring cover.
b. Using a Phillips screwdriver, loosen the captive screw at the front of the cuvette
loader assembly.
c. Remove the cuvette loader without disconnecting the ribbon cable and set it
aside.
d. Remove the plastic incubation ring cover.
e. Replace the cuvette loader.
2. At the Probes Calibration window, select Init to home the system subassemblies.
Figure 17. Sample Probe Incubation Ring Area (calibration point 10)
Figure 18. Sample Probe Incubation Ring Coordinate (calibration point 10)
b. Select OK.
The incubation ring moves the additional calibration pins beneath the probe.
c. Repeat this procedure for two additional pins on the incubation ring.
For these additional pins, the probe is calibrated to the same x- and y-coordinates
saved for the first point. Calibrate the probe to the z-coordinate only. After the
probe is calibrated to the third calibration pin, the probe moves to the home
position.
5. Replace the plastic incubation ring cover:
NOTE: If you will be calibrating the reagent probe to the reagent probe incubation
ring coordinate, skip these steps now, and perform them after calibrating the reagent
probe.
a. Remove the cuvette loader without disconnecting the ribbon cable and set it
aside.
b. Replace the plastic incubation ring cover.
c. Replace the cuvette loader.
Figure 19. Distance between reagent probe tip and bottom of probe housing
CAUTION
Do not select the OK button unless the calibration is complete for that coordinate. If you
need to stop the calibration before completing the procedure, select Cancel. Selecting OK
prior to completing the procedure will overwrite the previously calibrated coordinates,
and the probe could be damaged.
CAUTION
Do not attempt to re-calibrate a probe if the probe crashes (goes offline) during the
calibration procedure. This could result in damage to the probe. If the probe crashes,
select Cancel to exit the window.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
2. Calibrate the reagent probe to the system reference point (calibration point 8):
b. Select OK.
The probe moves to the home position.
Calibrating the Probe to the Ancillary Reagent Area Coordinates
Calibrate the reagent probe to calibration points 11, 12, and 13 in the ancillary reagent
area. The ancillary reagent area is located on the left side of the reagent compartment.
Figure 21. Ancillary Reagent Area (calibration points 11, 12, and 13)
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
1. Remove the top shutter of the reagent compartment.
If you remove the front panel to gain a better view of the calibration points, ensure
that you reconnect the front panel connector before calibrating the reagent probe to
the incubation ring position.
2. Open the reagent compartment door.
3. Load both ancillary reagent trays into the area without reagent packs.
CAUTION
Do not move the probe back and forth over the pin. This could result in damage to the
Teflon probe lining.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
Figure 23. Primary Reagent Area (calibration points 14, 15, and 16)
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
b. Select OK.
CAUTION
Ensure that the bottom of the housing is 101 mm (3.98 in) from the top rim of the rinsing
station. Failure to calibrate this measurement exactly may cause the system to crash.
WARNING
Ensure that you are clear of subassemblies that can move before selecting OK. Remove
yourself from the machine. The probe moves rapidly and can cause injury.
CAUTION
Ensure that the probe is calibrated to the system reference point before proceeding. If the
probe is not properly calibrated to the reference point, damage to the probe could result.
1. If necessary, remove the plastic incubation ring cover:
a. Using a Phillips screwdriver, loosen the captive screw at the front of the
incubation ring cover.
b. Using a Phillips screwdriver, loosen the captive screw at the front of the cuvette
loader assembly.
c. Remove the cuvette loader without disconnecting the ribbon cable and set it
aside.
d. Remove the plastic incubation ring cover.
e. Replace the cuvette loader.
2. If necessary, select Init to home the system subassemblies.
3. At the Probes Calibration window, select the reagent probe incubation ring
coordinate area.
The reagent probe moves to the reagent probe incubation ring area.
Figure 30. Reagent Probe Aligned to the Reagent Probe Incubation Ring
Calibration Point
b. Select OK.
The incubation ring moves the additional calibration points beneath the probe.
c. Repeat this procedure for three additional points on the incubation ring.
For these additional points, the probe is calibrated to the same x and y
coordinates saved for the first point. The probe only needs to be calibrated to the
z coordinate. After the probe is calibrated to the fourth calibration point, the
probe moves to the home position.
5. Replace the plastic incubation ring cover:
a. Remove the cuvette loader without disconnecting the ribbon cable and set it
aside.
b. Replace the plastic incubation ring cover.
c. Replace the cuvette loader.
Trademark Information
ADVIA Centaur is a trademark of Siemens Medical Solutions Diagnostics.
Appendix
Probes Calibration Checklist
Use this checklist when calibrating the probes to the calibration points.
No. Probe Area Calibration Point Done
8 Sample probe System reference point System reference point
1 Tip drawers Left-back
2 Left-front
3 Right-back
4 Right-front
5 Tip eject area Back eject position
5 Front eject position
5 De-acceleration position
6 Sample compartment Left-back
7 Left-front
8 Right-front
9 Lane 7, position 10
10 Incubation ring Sample probe position
8 Reagent probe System reference point System reference point
11 Ancillary reagent area Left-back
12 Left-front
13 Right-front
14 Primary reagent area Left-back
15 Left-front
16 Right-front
17 Rinsing station Rinsing hole down
17 Rinsing hole up
18 Drain hole
19 Incubation ring Reagent probe position