Read the method, if you can print highlight and annotate to pick out key points of interest then

answer the questions

Questions Part 1
1. What term could you use to explain why DCPIP becomes colourless. It has been ……….

2. What do you think might be in the isolation medium that is used to make the choroplast
suspension?

3. Why is everything kept cool using ice?

4. What is the purpose of blending the torn pieces of leaf

5. What would you do if you found large pieces of leaf at the end of step 6?

6. For each tube note down why you think you are setting each up.

7. What is the purpose of the repeats for tubes X and Y?

8. Look at alternative steps 9-13. This is normally the approach we follow in lessons. Why
might this be an improvement on the standard method?