LAMPIRAN

Lampiran 1 Perhitungan % rendemen HAp

 mol Ca(OH)2
𝐵𝑜𝑏𝑜𝑡 Ca(OH)2 3,75
mol Ca(OH)2 = = 74 𝑔/𝑚𝑜𝑙 = 0,05 mol
𝑀𝑟

 mol (NH4)2HPO4
𝑀 (NH4)2HPO4 0.3 𝑀
mol (NH4)2HPO4 = = 0,1 𝐿 = 0,03 mol
𝑉

10Ca(OH)2 + 6(NH4)2HPO4 Ca10(PO4)6(OH)2 +12NH4OH + 6H2O

m 0,05 mol 0,03 mol - - -
b 0,05 mol 0,03 mol 0,005 mol 0,06 mol 0,03 mol -
s - - 0,005 mol 0,06 mol 0,03 mol

Massa Ca10(PO4)6(OH)2 = mol x bobot molekul

= 0,005 mol x 1004 gram/mol
= 5,02 gram
Bobot Ca(OH)2 Bobot (NH4)2HPO4 Bobot Ca10(PO4)6(OH)2 % rendemen
3,75 gram 3,96 gram 4,7402 gram 61,48%
Perhitungan :
Bobot Ca10(PO4)6(OH)2
Rendemen(%) = Bobot Ca(OH) x 100%
2+Bobot (NH4)2HPO4
4,7402
= x 100%
7,71

= 61,48%

Lampiran 2 Penentuan kadar kalsium dalam HAp

VCa2+ VEDTA (mL) [EDTA] [Ca2+] Kadar
Ulangan
(mL) Awal Akhir Terpakai (M) (M) Ca (%)
1 5 0,10 6,60 6,50 0,00383 0,00498 1
2 5 6,60 12,70 6,10 0,00383 0,00467 0,93
3 5 12,70 18,60 5,90 0,00383 0,00452 0,90
Rerata 0,94
SD 0,05
Ketelitian 94,68%
Contoh perhitungan (ulangan 1):
 Rerata kadar Ca
𝑋𝑖
x =∑𝑛𝑖=0 𝑛
(1+0,93+0,90)%
= 3

= 0,94%
 Standar deviasi
2 2
n
∑ (xi- x) (1-0.94) +(0.93-0.94)2 +(0.90-0.94)2
SD = √ i=0 n-1 =√ 3-1

= 0,05
 Ketelitian
SD
Ketelitian = (1 − Rerata) x 100%
0.05
= (1 − ) 𝑥 100% = 94,68%
0.94
 Penentuan konsentrasi molaritas Ca2+
(𝑀 𝑥 𝑉)
[Ca2+] = 𝑉 𝐸𝐷𝑇𝐴
𝐶𝑎2+

6,50 𝑚𝑙 𝑥 0,00383 𝑀
= 5 𝑚𝑙
= 0,00498 M
 Penentuan kadar Ca
𝑀𝐶𝑎2+ 𝑥 𝑉𝑒𝑘𝑠𝑡𝑟𝑎𝑘 (𝐿)𝑥 𝑀𝑟 𝐶𝑎
%Ca = 𝑥 100%
𝑔 𝑆𝑎𝑚𝑝𝑒𝑙
0,00498 𝑀 𝑥 0,005 𝐿 𝑥 40 𝑔/𝑚𝑜𝑙
= x 100%
0,1 𝑔𝑟𝑎𝑚
=1%

Lampiran 3 Kurva standar fosfor

0,25
0,2 y = 0,0741x - 0,0045
R² = 0,99
0,15
Absorbans

0,1
0,05
0
-0,05 0 0,5 1 1,5 2 2,5 3 3,5
Konsentrasi standar [M]

Lampiran 4 Penentuan kadar fosfor dalam HAp

Ulangan ppm Volume (L) Bobot sampel (g) FP Kadar P (%)
1 1,321 0,1 0,10 1000 1,321
2 1,080 0,1 0,10 1000 1,080
Rerata 1,2005
SD 0,17
Ketelitian 85,84%
Contoh perhitungan (ulangan 1):
 Rerata kadar fosfor
𝑋𝑖
x =∑𝑛𝑖=0 𝑛
(1,321+1,080)%
= 2

= 1,2005%
 Standar deviasi
2
∑𝑛
𝑖=0(𝑥𝑖− 𝑥) (1,321−1,2005)2 +(1,080−1,2005)2
SD =√ = √
𝑛−1 2−1

= 0,17
 Ketelitian
SD
Ketelitian = (1 − Rerata) x 100%
0,17
= (1 − ) 𝑥 100% = 85,84%
1,2005
 Penentuan bobot fosfor
𝑝𝑝𝑚 𝑥 𝑉(𝐿) 𝑥 𝐹𝑃
Bobot P = 𝑏𝑜𝑏𝑜𝑡 𝑠𝑎𝑚𝑝𝑒𝑙 𝑥 10
1,321 𝑝𝑝𝑚 𝑥 0,1 𝐿 𝑥 1000𝑥0,001 𝑔/𝑚𝑔
= 0,1 𝑔 𝑥 100
=0,01321 gram
 Penentuan kadar fosfor
%P = bobot P x 100%
= 1,321%

Lampiran 5 Nisbah Ca/P dalam HAp

% 𝐾𝑎𝑑𝑎𝑟 𝐶𝑎 0,94
=
% 𝐾𝑎𝑑𝑎𝑟 𝑃 1,2005

= 0,78
Lampiran 6 Perhitungan % rendemen TCP
 mol Ca(OH)2
𝐵𝑜𝑏𝑜𝑡 Ca(OH)2 3,75
mol Ca(OH)2 = = 74 𝑔/𝑚𝑜𝑙 = 0,05 mol
𝑀𝑟

 mol (NH4)2HPO4
𝑀 (NH4)2HPO4 0.4 𝑀
mol (NH4)2HPO4 = = 0,1 𝐿 = 0,04 mol
𝑉

3Ca(OH)2 + 2(NH4)2HPO4 Ca3(PO4)2 + 4NH3 + 6H2O

m 0,05 mol 0,040 mol - - -
b 0,05 mol 0,040mol - 0,016 mol 0,064 mol 0,096 mol +
s - - 0,016 mol 0,064 mol 0,096 mol
Massa Ca3(PO4) = mol x bobot molekul
= 0,016 mol x 215 gram/mol
= 3,44 gram

Bobot Ca(OH)2 Bobot (NH4)2HPO4 Bobot Ca3(PO4) % rendemen

3,75 gram 5,28 gram 5,0523 gram 55,95%
Perhitungan :
Bobot Ca10(PO4)6(OH)2
Rendemen(%) = Bobot Ca(OH) x 100%
2+Bobot (NH4)2HPO4
5,0523
= x 100%
9,03

= 55,95%

Lampiran 7 Penentuan kadar kalsium dalam TCP

VCa2+ VEDTA (mL) [EDTA] [Ca2+] Kadar
Ulangan
(mL) Awal Akhir Terpakai (M) (M) Ca (%)
1 5 19,50 26,60 7,10 0,00383 0,00544 1,088
2 5 26,60 33,00 6,40 0,00383 0,00490 0,98
3 5 33,60 40,30 6,70 0,00383 0,00513 1,026
Rerata 1,031
SD 0,05
Ketelitian 95,15%
Contoh perhitungan (ulangan 1):
 Rerata kadar Ca
𝑋𝑖
x =∑𝑛𝑖=0 𝑛
(1,088+0,98+1,026)%
= 3

= 1,031%
 Standar deviasi
2
∑ni=0(xi- x) (1,088-1,031)2 +(0.98-1.031)2 +(1,026-1,031)2
SD =√ =√
n-1 3-1

= 0,05
 Ketelitian
SD
Ketelitian = (1 − Rerata) x 100%
0.05
= (1 − ) 𝑥 100% = 95,15%
1,031
 Penentuan konsentrasi molaritas Ca2+
(𝑀 𝑥 𝑉)𝐸𝐷𝑇𝐴
[Ca2+] =
𝑉𝐶𝑎2+

7,10 𝑚𝑙 𝑥 0,00383 𝑀
= 5 𝑚𝑙
= 0,00544M
 Penentuan kadar Ca
𝑀𝐶𝑎2+ 𝑥 𝑉𝑒𝑘𝑠𝑡𝑟𝑎𝑘 (𝐿)𝑥 𝑀𝑟 𝐶𝑎
%Ca = 𝑥 100%
𝑔 𝑆𝑎𝑚𝑝𝑒𝑙
0,00544 𝑀 𝑥 0,005 𝐿 𝑥 40 𝑔/𝑚𝑜𝑙
= x 100%
0,1 𝑔𝑟𝑎𝑚
= 1,088 %

Lampiran 8 Penentuan kadar fosfor dalam TCP

Ulangan ppm Volume (L) Bobot sampel (g) FP Kadar P (%)
1 1,442 0,1 0,10 1000 1,442
2 3,239 0,1 0,10 1000 3,239
Rerata 2,3405
SD 1,27
Ketelitian 45,73%
Contoh perhitungan (ulangan 1):
 Rerata kadar fosfor
𝑋𝑖
x =∑𝑛𝑖=0 𝑛
(1,442+3,239)%
= 2

= 2,3405%
 Standar deviasi
2
∑𝑛
𝑖=0(𝑥𝑖− 𝑥) (1,442−2,3405)2 +(3,239−2,3405)2
SD =√ =√
𝑛−1 2−1

= 1,27

 Ketelitian
 SD
Ketelitian = (1 − Rerata) x 100%
1,27
= (1 − ) 𝑥 100% = 45,73%
2,3405
 Penentuan bobot fosfor
𝑝𝑝𝑚 𝑥 𝑉(𝐿) 𝑥 𝐹𝑃
Bobot P = 𝑏𝑜𝑏𝑜𝑡 𝑠𝑎𝑚𝑝𝑒𝑙 𝑥 10
1,442𝑝𝑝𝑚 𝑥 0,1 𝐿 𝑥 1000𝑥0,001 𝑔/𝑚𝑔
= 0,1 𝑔 𝑥 100
=0,01442 gram

 Penentuan kadar fosfor

%P = bobot P x 100%
= 1,321%

Lampiran 9 Nisbah Ca/P

% 𝐾𝑎𝑑𝑎𝑟 𝐶𝑎 1,031
=
% 𝐾𝑎𝑑𝑎𝑟 𝑃 2,3405

= 0,44

Lampiran 10 Spektrum FTIR HAp

2-
CO3

3- 3-
PO4 PO4
Lampiran 11 Spektrum FTIR TCP

OH

2-
CO3

-3 3-
PO4 PO4