An enzyme is a protein molecule that is defined as a biological catalyst , which is a substance that speeds

up chemical reactions but remains unchanged at the end of the reaction. The rate of reaction of an
enzyme controlled reaction varies with different temperature. As temperature increases, the height of
the foam will gradually increase until its optimum temperature is reached. This is as a result of as the
temperature increases, the particles, which are enzymes and substrate molecule, gain more energy due
substrate reactions happening more often. Between 25 oC - 35oC the height of the foam gradually
increases. The optimum temperature of an enzyme is usually approximately 37 oC, thus the height of the
foam will steadily increase, this mean s the enzyme’s catalytic activity is at its greatest. It is expected
that most of the product to be produced at the optimum temperature will be the greatest of the
enzyme. Temperatures after the 37oC will produce the lowest values for the height of foam as the
enzyme has now become denatured. Once the enzyme has reached its optimum temperature and as the
temperature continues to increase, it is expected that the height of the foam decreases as the enzyme is
now become denatured. This means that the enzyme molecule now begins to lose its shape and the
substrate molecules does not fit well into the active site of the enzyme anymore. This results in the rate
of reaction slowing down until the reaction stops. The heights of the foam are measured to illustarate
the effect of temperature on an enzyme-controlled reaction. It also highlights the amount of product
formed. The graph plotted is expected to look like a maximum curve, if this is obtained, the hypothesis is
valid, otherwise, the hypothesis is deemed as invalid.