Linkers are short DNA sequences that are used to join two DNA fragments together. They contain restriction enzyme sites that allow the DNA fragments to be cut and ligated together. Common linkers used include T4 DNA ligase which catalyzes the formation of a phosphodiester bond between the 3'-hydroxyl end of one DNA strand and the 5'-phosphate end of another.
Linkers are short DNA sequences that are used to join two DNA fragments together. They contain restriction enzyme sites that allow the DNA fragments to be cut and ligated together. Common linkers used include T4 DNA ligase which catalyzes the formation of a phosphodiester bond between the 3'-hydroxyl end of one DNA strand and the 5'-phosphate end of another.
Linkers are short DNA sequences that are used to join two DNA fragments together. They contain restriction enzyme sites that allow the DNA fragments to be cut and ligated together. Common linkers used include T4 DNA ligase which catalyzes the formation of a phosphodiester bond between the 3'-hydroxyl end of one DNA strand and the 5'-phosphate end of another.