Oxidation Sanitation
Chase Henderson
Introduction
During the pandemic, many problems sprouted about Materials
wearing masks everyday. So, a question came to mind,
would there be a way to sanitize masks quickly and
efficiently? That’s when activated oxygen came to mind.
5 petri dishes
Method(s)
People use it all the time to sanitize CPAPs so why couldn’t
this be used to sanitize regular masks as well? In the article
Inactivation of Viruses and Bacteria by Ozone, With and
Without Sonication it states,
“Bacteria suspended in PBS, were completely
inactivated after 15 s of treatment with ozone alone(p.341)”
The purpose of this project was to create an engineering
design that would use ozone as a form of sanitation for
masks to ensure that masks can be cleaned quickly and
efficiently.
Objectives
20
18
⬜Design an ozone sterilizer that will be
able to sterilize objects in a short period of 16
time 14
▪ The ozone sterilizer should be able to sterilize in around
15 to 20 minutes. 12
⬜Use petri dishes that will go into the 10
ozone sterilizer at different times as testing
8
• The petri dishes will go in at 5 minutes, 10 minutes, and 6 the less
15 minutes and then be pulled out and left to see what
grows. 4 bacteria
⬜Come back to the petri dishes after about 2
a week and determine the growth of the 0
bacteria of each of the petri dishes
Materials and Methods Conclusion
● SANIWISE Multi-Purpose Ozone Sterilization and Disinfection Machine ⬜The longer the Petri dishes were in the ozone
● Dap 2.8 oz. Silicone Auto/Marine Sealant sanitizer the less bacteria grew on the petri dish.
● Sterilite 51-Quart (48 Liter) Modular Latch Box
●
▪ The longer the ozone was in contact with the
petri dishes the more bacteria it killed.
1. The first step is to cut out a piece of the wood that will be nailed into the container and
will hold the ozone generator ⬜The bacteria changes from petri dishes seems
2. The second step is to bolt in the wood and put nails into the wood that holds up the
ozone generator
to decrease from one petri dish to another
3. The third step is to make two holes in the container one at the bottom of the side with
the ozone generator and one at the top of the opposite side.
▪ This suggests that the less bacteria there is on
4. The fourth step is to attach the plastic tube to the ozone generator that will transfer the the object the less effectively it will sanitize
activated oxygen into the plastic container and apply the sealant.
5. The fifth step is to take a mask and swab it out into different petri dished that will go ▪ There will not be a way to sanitize absolutely
into the ozone sterilizer for different amounts of time and then let them sit to see what 100% of the bacteria
grows.
⬜The control in the experiment grew much less
Ozone Sanitation than what the other data would suggest it grows.
▪ The container of the ozone sanitizer might have
had bacteria that got into the petri dishes while
⬜The it was running
control only ▪ There might have been a mistake with putting
grew 3 the swab of bacteria from the used mask into
bacteria the petri dish
colonies
⬜The longer
the petri
dish was in
the ozone
there was.
References
1 2 3 4
Petri Dishes(min in ozone) Bacteria Colonies Burleson, G. R., Murray, T. M., & Pollard, M. (1975, March
1). Inactivation of viruses and bacteria by ozone, with and without
Sonication. Applied and Environmental
Microbiology. https://aem.asm.org/content/29/3/340.short.