Supplementary Information

Kinetic and Mechanistic Parameters of Laccase Catalyzed Direct

Electrochemical Oxygen Reduction Reaction
Naga S. Parimia, Yogeswaran Umasankara, Plamen Atanassovb and Ramaraja P. Ramasamya,*
a
Nano Electrochemistry Laboratory, Nanoscale Science and Engineering Center and Faculty of Engineering, University of
Georgia, Athens, GA 30602.
b
Center for Emerging Energy Technologies, Department of Chemical and Nuclear Engineering, University of New Mexico,
Albuquerque, NM 87131.
   

 
 

Figure A1: Optimization of the bio-nanocomposite preparation by comparing the cyclic voltammograms in oxygen
saturated electrolyte; scan rate 10 mV s-1. (a) CVs for different MWNT loadings on electrode. (b) CVs for different
concentrations of the crosslinker (PBSE) used for electrode incubation. (c) CVs for different concentrations of laccase
solution used for electrode incubation. (d) Bar chart comparing the open circuit potentials and the half-peak potentials
obtained for different loadings of PBSE and laccase. 0.8 mg cm-2 of MWNTs were loaded in each case. Based on the graph,
the optimized concentrations for PBSE and laccase are 5 mM and 10 mg mL-1 respectively.
Figure A2: Comparison of the ORR voltammograms of a highly active and less active bio-nanocomposite. Scan rate, 10 mV
s-1.

Figure A3: Plots to obtain the slopes and intercept values used for the calculation of rate constants K1, K2 and K3. (a) Plot of
ID/IR versus ω-1/2 to determine the parameters S1 and I1 (b) Plot of IDL/(IDL-ID) versus ω-1/2 to determine the parameter S2.