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    4 views3 pages

    Electrolyte Procedure

    Uploaded by

    Chintya Jessica

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
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    Sopium rapid Photometric Determination of Serum Sodium Mg-Uranylacetate Method, Colour Test fackage site = ma am lin igritearce Sedum measurements ae used the eign an restentet est tareeref fad sno vectchte bvanceeg due en of nate! 03% 1nd ater sr eels deviting or the rfernc rer by Fotyneoiytypscyaiomes ard gare ss ena doses, per {erin dacren ef the acaba Blac, some ercactine Gees tdems exceed rah Intended Use Colcretn test or he quantitative determination of sou a human Serum andhepanned pavna Method Sodium ie presptted wth Mg urany! acetate; the uany/ ions erating In surpentan form a yellow brown complen uth tunes ac. The Gievene Belwcer reagent blak fwthout precipitation of so8 ur) aed nays i propotona tothesodumconcertstion Cortents, Reagent Composition For20 mace: / 60 sem mcr detentions (REC) Om Precintating solution Uransacetate rsmmaif Iagnesum acetate xcmmal comiColour reagent ‘rimanivm hapicoste ss0mmalf ammona s50-mmol/ 2ral standard Scauum pia) sommal/ stasity Stored unogenes st rom terperotute (25.25) and in the ear the Eontenisone robe url te expiry Ste prints one el Specimen Assay iavelensth: Hg 3650. Hedasnm, 100m Optesipath dem Temperature: 20.25 Ncasiement, The photometer is sctond against dstled water. OD fevuls must be cote forthe fagent Slane (88). niyone BB perseses seequred pete myca "7 Seniemice We oe some Repeagent and standard Ha) tw) fo) = = 50 x0 sewn rene) 2» oa) J boca {3000 1000 _ 3000 ‘Clee tubet and min lel Arete staATENS minutes. Shake inten ‘vl for at east 30 ee, toto stand fo 30 min Centr fugest high Speetforsl0mnutes a == a Gear supeteatant cay (507 2%» at] aca0 PRO. seni 000 1000 | 1000 sll Ate 5.30 minutes, meosue SBhrbance of F(x he standate (srl andthe sample (8m) gait distil water at 360-10 Pg S65 oe 05). Gslevaton (aerated cose Se immo (heats) sual = mmol Peformance Chota Linearity With sodium concentrations exceesing 209 mol/l the serum must be precited 1+ thirties ater Matis the estoy 2 Typ perfermance data can te found inthe Venfcaion Report acces: Bev wwe human deféata/b/eley 9 pat ew human de com/sata/ebierleyna pot Ifthe pevorance cata ar not accel vis ntene, they can be tained fe of chorge fom your aalestnbutr Reference Vales sera 135-155 mmoV/ ‘uatty conto! [ll contol sera with sodium values determined by this method can be enployes ‘Werecommendto use ou Huma qualtyconl sera based cn arial ‘serum ur SER0DOS based en human Stu, ater 1 Usethe ern pocedate only if avery efficient centage (8000 = Sood) if avalable Otnennze use the Maceo precedue to obtain telabie resus 2 (FREE becomes dscloued wien exposed tothe ight Stove protected Fromi git sight tubudity does et atecttne determination 4. Detergents usally contain high sodium concentrations. The equip trent tes takes pipettes tapers, cuvettes -must therefore be wnsed Detallywith dtiled water Avod contamination by aces of sodium ‘set 4 Dipoeable plastic tubes are ecormerded forthe determination Use Partin of plasic stoppers tacos the tubes. Safety Notes Warning Hatard statements av6Rlarmapie quid ond vapour i02 Harm if snowed. Precautionary statements 210 Keep any fom neatlsptisfopenfamesnt surfaces. Ne smal i 241 Us explosion proof electriaventiating/ghtng/equipment F240 Wear protective gleves/protective clothingJeeprotectiontace protection aga/3016¥398 # Gus Sut [or na}. Hemove/ ake ott meaty Contaminated caving Rinse sunt water/shower F501 Dapose of contentsfcontainer in accordance ith lo caliegonalnatonal/nterrationaegustons Horard statements Ha0z Homi swallowed S14 Caunes severe sin burns and eye aamage F285 May aut espiratry entation Precautionary statements 7303403614P359 FON SN fr ai), Remove/Take off immecitey all contaminate clothing Fine sin wl ates 7305+2351¢P338 IF IN EYES: Rinse cautious with water for several minutes, Remove contact lenses present and sy t0 eo Continue resin Pa10Immedatelycall 2 POISON CENTER/docta. PAS Stove laced up, F501 sose of contensfeontaner ia accordance with lea Pregional/saanafintenatiorai regula ns References 1 Hinder, Ansist 75,9 (2959) 2 Hany RJ eta, Clin Chem, Harpe & ow New Yor, See fait 63 sri Human oo pln an Canscaner Crtonripe liquicolor Photometric Colorimetric Test for Chloride TPTZ Method Package size a ons 200m a ethos! Chloride sons react ith a mercy 2464 omsples to fom mercury ehstde the iterated 7 ‘rit ying» blue eotnred comple The sling abso Fst hide 5 completetestet 2-pyidl tiaine (172) ges 90 nov duct proportonat a the mtnecinple Contents at} 2200 Coloureeagent ipa ae mercy camps! os96 mmol Wi slphate ass nmi [ER] amistandna enoagetet). 00 mel SssmevAl Seagent Preparation Eaton Sta ae rendytouse Reagent Sabiy re stble even ater opening upto ie stated epi date Bec me cath Contamnnatin unt be vee! specimen serum. C56, urine Stayin serum at 2 Teays stapiy unre 2 Tans Blond shouldbe immediate poset fog cents fuged within 3 hur tierra Sete car econ sean ae aR mY Fresittes in wins maybe recessed by armang op. Ay invelersth: $80.00 (860- 600mm) 49578 am Sptesipath Dem Tenipersture 20.3 Sevsurement gest 1 ques ert blink Only one Blank per sere ie Pipettng Scheme facia method reste SYE) arte sample 1 + 40 with died water, Cp SOul[STaysampie« 2000 last water iio method edit EYE) and the sample x +50 with duties wate p 20 S)/sample 1009 last water Use the prea mpe forthe following pipetting severe. Ppeteinto Hocr0 Semisniro cine BI Sample Sam es sox! 204! Sample 50H 2041 zoveul 20001 1000412000 ncaa or Sites the dark and measure Deabsorbace cf Sample (eo) and (ST) (Aan 20 minutes against he reagent anh Oo eat expose eh c= 1002 tet co 3554 Te esl nay be converted according tothe folowing equaens ¢lmel) = elma clot x358 = elme/el clmgld'x0282 = [mot] pehurne: elmmalf)eurinevolume[/2SIN} = efmmay2ay} elmmol 24 H) 00355, ip2en esormanee Chaactersties Uireaity the test is neat up toa chloe concentration of SCO msi 1775 Iry/al Somes uth higher chloe concentration have tobe further Gibted 3 «4 nithdstifed water Repeat te assay ard matty the es by 2, Types) perfomance data can be found the Vewtcation Fepo. wor human de/ata svn huranscecom/astalgb/tleyc pat it the performance dato are nt accesible via internet they can be ‘blaine ee of hare rom you esa éstributo Normal values Serum, 95-10Rmmol 335-383 maid cs 119-230mmold 482-400 Ra ine, 110-225enmol 3-8/2 Cuslty Cont [A contol sero with cide values determined by ths method can be ‘ie tcervment to use our cualty contol sera HumaTit based on {nial serum ou SEEODOS based on human sium Automaton Proposals to a9phi the reagents on analyzers ave avaabe on rea Exehisboater hata vatetethe application in ts own responsibly i cts iad very sensteely Usage ef contaminated assnoie sna sin corttshersiowe can cause @vaiea crows Sut Use of ceposale.plaste. wave and. gloves 6 hh recommensed 2.Hemoglcbin, tinbin, its and ascorbic ac in physiological concession ds not terterewth the test 2 EM ses 1 a, expecay WY at pvt rote tet 3 should be hep Safety Notes ring! Horatd statements E72 May cause damage to ovgars though ptolonaed or repeated espsve Precautionary statements 260 Bow breathe dustifume/gas/mistvapoursisay 214 cet meicslasie/atertion# you fal uel 501 Dispose of contentafcortainr in acendance with Ical/egionlf national) ntevnationl regulate References Palit W Klinich-cheische Diagnosis, Teme Stuttgart, 2.68 we 2. Fred ett. 1 Cin. Chem, Gi Biochem 20,280(9725 ce Human red pln an Canscaner Catcium liquicolor Photometric Test for Calcium CPC Method Package sire roo 200m Compete test hit fi} Method! Calcium tons react with octescphthalincomplesene in an altaine medium to form a puple colored complex The absorbance of ths Compices proportional tote alum concentration ithe sample Contents [EE] 100 Buffer Solution lysine buffer gh a0 2 o2neil Sedum ade 095% 260 ml Colour Reagent SHysronjauiotne Lumet ©-Crexolphthaln-complexene Or meet Syareenon ae somrel/ amisteneaes Gileum@ Emg/tion? mot Sedum ade 0095 Reagent Preparation 2a [EG] 0 [5] i equal volumes 25 require, enix and alow to stand e130 minutes troam temperature Deore we Reagent staity Thecagnts ov the sanead are sale even afer opening wp othe ted esp date ter stored af 2-250 contami us Be Noses ‘ The working reagent stable for 7 days at 2.8°C and for 3 days at specimen Serum, eparinies plans Stabity inserum 10 day. at 2.25% Ay ‘optical path: em Temperature, 20.25°C teasurerent. Against agent Blank Only one reagent bat per sees 70m He 780m, Se em istequed Pipetting Scheme Pipette ocuvettes Reagent blak sample on ETB] Sample o 0) 204 Wioring regent 1000 1000 taixand measure the absorbance sample (spe) and standard atcehaga nat he reagent Bank within to 30 mirutes Calelation ofthe Cate Concentration Soe tengia) Pevformance Characteristics Uineatity The test is linear up to 2 caloum concentration of 15 male of 275 miro. Sarapes with a higher concentation have to be ated 2+ thaistiled wate opest the assy a multiply the vsults by 2 Typist perfermance date can be found in the Veuifction Report ww human ge/data/eblfeyca per seve humane com/data/gbhrfey

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