Tyrosinase

from mushroom

Catalog Number T3824

Storage Temperature –20 C

CAS RN 9002-10-2 Unit Definition: One unit will cause an increase in A280
EC 1.14.18.1 of 0.001 per minute at pH 6.5 at 25 C, in a 3 ml
Synonyms: Monophenol monooxygenase; reaction mix containing L-tyrosine.
Monophenol, dihydroxyphenylalanine:oxygen
oxidoreductase Precautions and Disclaimer
This product is for R&D use only, not for drug,
Product Description household, or other uses. Please consult the Safety
Tyrosinase is a copper-containing oxidase which has Data Sheet for information regarding hazards and safe
activity on both catechols and cresol. It is responsible handling practices.
for browning reactions. Tyrosinase is reported to have
two binding sites for aromatic substrates and a different Preparation Instructions
1
binding site for oxygen, the copper-containing site. Tyrosinase is soluble (2 mg/ml) in 50 mM potassium
phosphate buffer, pH 6.5, yielding a clear brown
Overall Reaction: solution.
O O O O
H2N CH O OH H2N CH O OH H2N CH O OH H2N CH O OH
Storage/Stability
CH2 CH2 CH2 CH2
When stored at –20 C, the product loses 5% activity
O2
Tyrosinase
H2 O per year. If stored at 37 C, 40–60% activity is lost in
7
7 days.
OH OH O
OH OH OH
Solutions retain activity for several days at 4–8 C and
O

L-Tyrosine L-DOPA L-DOPA L-DOPA Quinone

for several weeks frozen at –20 C. The enzyme is
6
4
KM for L-Tyrosine: 0.5 mM
2 rapidly denatured in 1% SDS solution. It dissociates
2
KM for L-DOPA: 1.5 mM into subunits in saturated urea and is completely
inactivated. While the dissociation is reversible, the
6
Catechol acts as a suicide substrate for tyrosinase, inactivation is not.
causing irreversible inactivation during catechol
3
oxidation. Tyrosinase is also inhibited by compounds References
that complex with copper, such as benzoic acid and 1. Duckworth, H.W., and Colman, J.E., J. Biol. Chem.,
cyanide. Benzoic acid inhibition is competitive with 245(7), 1613-1625 (1970).
catechol. Cyanide inhibition is competitive with oxygen 2. Espin, J.C. et al., J. Agric. Food Chem., 47(9),
and noncompetitive with catechol.
1 3495-3502 (1999).
3. Cánovas, F.G. et al., Biochim. Biophys. Acta,
4
Molecular mass: 119.5 kDa (electrophoresis) 912(3), 417-423 (1987).
5
Isoelectric point (pI): 4.7–5 4. Gillespie, J.P. et al., Comp. Biochem. Physiol. Pt.
pH optimum: 6–7 C, 98(2-3), 351-358 (1991).
5. Robb, D.A., and Gutteridge, S., Phytochemistry,
This product is isolated from the mushroom species 20(7), 1481-1485 (1981).
6
Agaricus bisporus. The product was dialyzed against 6. Kertesz, D., and Zito, R., Biochim. Biophys. Acta,
deionized water prior to lyophilization. This enzyme is 96(3), 447-462 (1965).
also assayed for polyphenol oxidase and catechol 7. Kertesz, D. et al., Biochem. Biophys. Res.
oxidase activities. Commun., 49(5), 1208-1215 (1972).

RBG,GCY,MAM 03/18-1
Activity: 1,000 units/mg solid
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