4/21/2021 Labster proteins synthesis.

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Labster proteins synthesis. CELL1030

Terms in this set (34)

Do you and is a molecule of hereditary Transcription, translation

that contains instructions to build proteins.
In 1958, Francis crick describe the central
dogma of molecular biology explaining
the process of gene expression. Pick the
correct term to fill in the blank spaces of
the image.

Click view image to view the difference RNA is less reactive.

between the main characteristics of DNA
and RNA. Which statement is not correct?

The mRNA strand is synthesized in the 5 to C (option with OH in bottom left)

3 direction. The circles in the image
indicate the chemical group to which the
next base is attached. Which image has the
circle in the correct position?

DNA and mRNA synthesis proceeds in the Five prime and three prime refer to the position of carbon Adams in
five prime to three prime direction. What the sugar back bone
do five prime and three prime refer to?

Protein synthesis is performed by an RNA P

protein complex called the ribosome. As
you can see in the image, a ribosome is
compromised of three sites called E, P & A.
The site where tRNA adds its amino acids
to the polypeptide chain is...

Eukaryotic cells synthesize proteins in Ribosomes, 2

the..., Which is shown as number... In the
image.

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What are the sequences of three nitrogen Codon

containing base of RNA that code for one
amino acid called?

The biosafety cabinet is important for The personnel, the experiment, and the environment
physical containment. In this lab we will
use a biosafety cabinet class too. Who or
what does a level two biosafety cabinet
protect?

The tRNA shown in the diagram will be 5' - ACU

able to form a base pair interaction with
which mRNA?

Three types of RNA cooperate to perform tRNA, mRNA, rRNA

translation. A; read codons and deliver the
amino acids. B; carry the genetic
information from DNA. C; catalyze the
assembly of polypeptide chains. Which
type of RNA matches the a, B, and C
descriptions, respectively?

Have a look at the simplified depiction of a Bottom

DNA strand. Which strand is the template
strand?

Which is the correct mRNA sequence 5' - AUUACAAUGE GGG GUG CACGAA UGU CCU -3'
resulting from the EPO gene transcription
process?

What is the correct amino acid translated MGVH ECP

from the resulting mRNA?

The erythropoietin protein is 193 amino 582

acids long, that means the number of
nucleic acid in the mRNA molecule
including the initiator in terminator codons
are...

Downstream means near the three prime TGDR

end, so noticed that the first codon is not
metonine. What is the correct amino acid
sequence?

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Amino acids are linked by covalent Peptide bonds

peptide bonds. The reaction of a peptide
bond produces one water molecule.
Multiple amino acids are linked together
by...

A sequence of amino acids, or a Primary structure

polypeptide, grows from the N- terminus
to the C- terminus. This polypeptide chain
represents the primary structure of a
protein. The linear sequence of amino
acids within a protein is considered the...
Structure of the protein.

Which level of protein structure is formed Secondary

by the weak bonds between oxygen and
hydrogen atoms within the polypeptide
back down?

The main to secondary structures of B&A

proteins are alpha helices and data sheets
which Are marked by... And... In the image.

Based on the master bedroom at her 12 kDa

resort, what is the mass difference
between rhEPO expressed in CHO &
E.Coli?

Glycosylation sites are marked with a CHO

diagram of glycan. Based on the mass
spectrometry results, rhEPO is glycosated
in which expression system?

Based on the mass spectrometry results, 4

how many glycosylation sites exist in
rhEPO-CHO?

Bacterial cells often cannot produce Lack the machinery for making post transitional modifications
recombinant proteins that are identical to
Native mammalian proteins. The reason for
this is that bacterial cells...

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Recombinant proteins produced by E. coli Golgi apparatus

cells are not qlycosylated. This is because
E.coli cells do not possess

Based on the mass spectrometry results, 0.4

the masses of our rhEPO expressed in
CHO and E. coli are 30 kDa and 18 kDa,
respectively. We also know that the EPO
proteins expressed mammalian cells are
glycosylated. Therefore we can say that...
Of rhEPO express in CHO are
polysaccharides

Are mammalian expression systems better Yes, erythropoietin is best expressed in mammalian cells
than the bacterial systems for the
production of biologically active and
stable erythropoietin?

Mass spectrometry identities molecules Mass and charge

based on their

MALDI is an abbreviation for Matrix assisted laser desorption/ ionization

Macromolecule ionization methods, such Predict the identity of an unknown Protein

as MALDI MS, allow us to obtain a peptide
mass fingerprint that can be used to

X, Y, and Z refer to the parts of a mass Y,Z,X

spectrometer. X detects the number of
different ions. Y is where Ion production
from the sample takes place. Z separates
ions with different mass to charge ratio's.
Which diagram is the correct
representation of X, Y, and Z

Proteins samples are mixed with a matrix Ensures ionization of protein molecules
prior to analysis. What is the function of a
matrix in MALDI MS analysis?

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In mass spectrometry protein molecules II, III, IV

are separated based on their mass to
charge ratio. The data is recorded in a
histogram for analysis. Which of these
histograms are not coherent with the mask
spectrometry principal?

Which athlete is using rhEPO? A

EPO can increase production of energy by Red blood cell

increasing ... production

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