RESTRICTION ENZYMES

This is a DNA sequence to be analyzed.

5’-
AACGCTACCTAGGATCCACCGAATTCAATCGACACCCCATTACCAGATCGTA
AGCTTA
TTCTATTCTCGCGCTACGTACTTTTAAAGGATCCATCGGGTACGGAATTCAAT
CGGATCCA
CCTACGATTTAGCTAGGATCCAATCCGATCCATTCGGGCCTAGAATTCTTCAT
TCGAATTCA TTCGAGCTTTTTAAACGATCTTTAAACGTAC-3’
Answer the following questions using the following enzymes
Sticky ends:
BamHI: G’GATCC
EcoRI: G’AATTC
HindIII: A’GCTT
Blunt ends:
DraI: TTT’AAA

2. If you use BamHI, how many DNA fragments would you

obtain and what would be their sizes?
3. If you use EcoRI, how many segments would you obtain.
Segment sizes?
4. If you use HindIII, how many segments would you obtain.
Segment sizes?
5. If you use DraI, how many segments would you obtain.
Segment sizes?

6. If you use EcoRI and BamHI combined, how many segments

would you obtain. What are the segment sizes?
7. If you use EcoRI and HindIII combined, how many segments
would you obtain. What are the segment sizes?
8. If you use EcoRI and DraI combined, how many segments
would you obtain. What are the segment sizes?
9. If you use HindIII and BamHI combined, how many segments
would you obtain. What are the segment sizes?
10. If you use HindIII, EcoRI and BamHI combined, how many
segments would you obtain. What are the segment sizes?