TCVN TIEU CHUAN QUOC GIA TCVN 7924-1:200 ISO 16649-1:200 Xuat ban lan 4 VISINH VAT TRONG THUC PHAM VA THUC AN CHAN NUOI — PHUGNG PHAP DINH LUQNG ESCHERICHIA COLI DUONG TINH #-GLUCURONIDAZA — PHAN 1: KY THUAT DEM KHUAN LAC 6 44 °C Stf DUNG MANG LOC VA 5-BROMO-4-CLO-3-INDOLYL #-D-GLUCURONID Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of #-glucuronidase-positive Escherichia coli - Part 1: Colony-count technique at 44 °C using membranes and 5-bromo-4-chloro-3-indolyl f-D-glucuronida HA NOI - 2008TCVN 7924-1:2008 Léi néi dau TCVN 7924 -1:2008 hoan toan tung ducing vdi ISO 16649-1:2001; TCVN 7924-1:2008 do Ban ky thuat tiéu chudn quéc gia TCVNITCIF13 Phuong hap phan tich va lay mau bien soan, Téng cuc Tiéu chudn Bo Iuéng Chat luong 46 nghi, BO Khoa hoc va Céng nghé céng bé. TCVN 7924:2008 (ISO 16649) Vi sinh vat trong thye phdm va thie 4n chan nudi — Phutong phap dinh lugng Escherichia coli duang tinh B-glucuronidaza, bao gém cac phan sau: ~ TCVN 7924-1:2008 (ISO 16649-1:2001) Vi sinh vat trong thu ph3m va thie an chan nuoi - Phuong phap dinh lucng Escherichia coli duong tinh f-glucuronidaza — Phén 1: Ky thuat dém khudn lac 6 44 °C str dung mang loc va 5-bromo-4-clo-3- indolyl-f-D-glucuronid; ~ TCVN 7924-2:2008 (ISO 16649-2:2001) Vi sinh vat trong thue phém va thie an chn nuéi - Phuong phép dinh lugng Escherichia coli duung tinh B-glucuronidaza — Phan 2: Ky thudt dm khudn lac & 44 °C sit dung 5-bromo-4-clo-3-indolyl-D- glucuronid; TCVN 7924-3:2008 (ISO/TS 16649-3:2005) Vi sinh vat trong thuc ph&m va this 4n chan nu6i ~ Phuting phép dinh lugng Escherichia coli duong tinh f-glucuronidaza — Phén 3: Ky thudt tinh s6 6 xac suat fan nhat sir dung 5-bromo-4-clo-3-indolyl-B- D-glucuronid.TCVN 7924-1:2008 Léi gidi thigu Do tinh da dang ctia thye pham va thifc &n chén nudi nén phuong phap nay cé thé khong thich hop dén tg chi tit cho timg san pham cu thé. Trong truting hgp nay, c6 thé sit dung cac phuong phap khac ac trung cho timg san pham, néu hoan toan chi vi ly do kj thuat. Tuy nhién, cén o& géng ap dung phuong phap nay khi cé thé. Khi tiéu chun nay duge soat xét thi cén phai tinh dén moi thong tin lién quan dén pham vi ma phucng phap dém dia nay phai tuan theo va cdc nguyén nhan gay sai léch so vai phuung phap trong trusng hgp cae san phdm cu thé. Viéc hai hoa cac phuong phap thit cé thé khéng thye hién dudc ngay va déi vdi mot vai nhom sdn pham cé thé tén tai cdc tiéu chudn quéc té va/hode tiéu chugn quéc gia ma khéng phil hop véi tibu chuan nay. Thong thung khi cdc tigu chudn quéc té nhu vay duge soat xét, thi chung phai duoc sta d6i dé phi hgp véi tiéu chudn nay, sao cho cudi cling chi cin cac sai léch véi phuang phap dém dia nay la cac ly do ky thuat duce thita nhan.TIEU CHUAN QuG6C GIA TCVN 7924 -1:2008 Xuat ban ln 1 Vi sinh vat trong thuc pham va thie an ctran ntdi - Phuong phap dinh lugng Escherichia coli dudng tinh B-glucuronidaza ~ Phan 1: Ky thuat dém khuan lac 6 44 °C sir dung mang loc va 5-bromo-4-clo-3-indolyl B-D-glucuronid Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of -glucuronidase-positive Escherichia coli ~ Part 1: Colony-count technique at 44°C using membranes and 5-bromo-4-chloro-3-indolyl -D-glucuronide 1 Pham vi ap dung Tiéu chun nay qui dinh phuong phap dinh lugng Escherichia coli dudng tinh -glucuronidaza trong phdm thuc phdm hoae thie an chan nuéi, Phuong phap nay st’ dung ky thuat dém khuan lac sau giai doan phuc hii stt dung cde mang lgc va U & 44 °C trén méi truéng dc c6 chita thanh phan tao sdc 48 phat hién enzym (-glucuronidaza. CANH BAO - Mét sé ching Escherichia coli khéng phat trién duge 3 44 °C va cy thé la cac Escherichia coli am tinh B-glucuronidaza nhu Escherichia coli 0157 sé khéng phat hién duge. Cac tai ligu vién dan sau la rat cn thiét cho ap dung tiéu chudn. D4i vdi cac tai ligu vién dan ghi nam céng bé thi 4p dung phién ban duge néu. Béi véi céc tai ligu vin dan khong ghi nam céng bé thi ap dung phién ban méi nhat, bao gém ca cac sla di. TCVN 6507-1 (ISO 6887-1), Vi sinh vat trong thuc phdm va thie an chan nudi — Chuan bj mau this, dung dich huyén phi ban dau va cac dung dich pha loang thap phan dé kiém tra vi sinh vat — Phan 1 Cac nguyén tae chung dé chuan bi huyén phir ban dau va cac dung dich pha loang thap phan. TCVN 6404 (ISO 7218), Vi sinh vat trong thc pham va thie &n chain nudi - Yéu cau chung va hung dn kiém tra vi sinh vat.TCVN 7924-1:2008 3. Thuat ngif va dinh nghia Trong tiéu chudn nay st dung cdc thuat ngit va dinh nghia sau day: 34 Escherichia coli dong tinh B-glucuronidaza (fi-glucuronidase-positive Escherichia coli) Vi khugn & nhiét 46 44 °C hinh thanh cac khudn lac mau xanh dién hinh trén méi trudng trypton-mét- glucuronid (TBX) trong cae diéu kién duoc qui din trong tiéu chudin nay. 32 Binh lugng Escherichia coli dugng tinh B-glucuronidaza (enumeration of B-glucuronidase-positive Escherichia coli) Viéc xac dinh sé luong cdc don vj hinh thanh khugn lac (CFU) cla Escherichia coli duong tinh B-glucuronidaza dugé tinh trén millit hoz trén gam mau, khi phép thi va viéc tinh toan duce thuc hién theo quy dinh trong tiéu chuan nay. 4 Nguyén tac 4.1 Cay mét lugng xac dinh cla mu thir hoc mét lugng x4c dinh cila huyén phil ban ddu lén cac mang xenluloza dude phi trén thach glutamat khoang cai bién (MMGA), sau 46 ii 6 37 °C trong 4h Sit dung cae dung dich pha long thép phan ca mau thir hoac ca huyén pho ban dau cy vao hai dia 6i véi mdi d6 pha long, trong cling mét diéu kién. 4.2. DE phan lap, chuyén cdc mang tir giai doan phuc héi trén MMGA sang thach typton-mat- glucuronid (TBX), réi di 4 44 °C trong 18h dén 24 h. 4.3. S6 lung don vi hinh thanh khudn lac (CFU) cla Escherichia coli dueng tinh ()-glucuronidaze trén gam hoac trén mililit mau duce tinh tir sé lugng CFU cé mau xanh dién hinh (xem diéu 10). 5 Dich pha loang va mai trudng cay B6i véi cae phong thir nghiém hién hanh, xem TCVN 6404:2008 (ISO 7218:2007). 5.1. Dich pha long Xem TCVN 6507-1 (ISO 6887-1) hoac tigu chudn cu thé lién quan d&n sn pham can xéc dinh. 5.2 Méi trudng cay 5.2.1. Méi trudng phyc héi: Thach giutamat khoang cai bién (MMGA) o5.2.4.4 Thanh phan TCVN 7924-1:2008 Natri glutamat Lactoza Natri focmat La-}-xystin L{-)-axit aspactic L-(+)-arginin Thiamin Axit nicotinic Axit pantothenic Magie sunfat ngam bay phan tit nutic (MgSO,.7H,0) ‘SAt (II!) amoni xytrat* Canxi clorua ngam hai phan ti nudéc (CaCl,.2H,0) Dikali hydro phosphat (K,HPO,) ‘Amoni clorua Thach Nude 6,359 100g 0,259 0,029 0,02 g 0,024 g 0,001 g 0,001 g 0,001 g 0,100 9 0.0109 0.0109 0,900 g 259 9g dén 18g” 1.000 ml * Ham lugng st it nhat la 15 % (phan khdi lugng) Tuy thude vao site déng cia thach 5.2.1.2 Chuan bj Hoa tan amoni clorua trong nutéc. Cho thém cac thanh phan khac vao va dun dén séi. Néu cn, chinh pH a8 sau khi kh triing pH phaila 6,7 + 0,2, 6 25°C Chuyén cae Iugng dén 500 mi mai truéng nay sang cdc vat chtfa thich hap (6.10), Khir tring 10 min 6 nhiét do 115 °C trong néi hap ap luc (6.1). 5.2.1.3 Chuan bj cac dia thach Rét cdc lugng ti 12 ml dén 15 ml mdi truting nay vao cac dia Petri v6 tring (6.11) va dé cho dng dae, Lam khé céc dia thach [xem TCVN 6404:2008 (ISO 7218:2007)]. Cac dia nay cé thé bao quan duce dén 5 ngay 63°C + 2°C. Cac dia thach can phai di khé 4€ hoi nuéc khong xuat hign trong 15 min khi dan dich cdy (1 mi)TCVN 7924-1:2008 5.2.2. Méi truéng chon gc: Tach trypton-mat-glucuronid (TBX) 5.2.2.1 Thanh phén ‘San phdm thuy phan casein bang enzym 20,09 Mudi mat No.3 159 Axit §-bromo-4-clo-3-indolyl-f-O-glucuronid (BCIG) 144 mol * Dimetyl sulfoxit (OMSO)? 3 mi Thach 9g dén 189° Nuéc 1.000 mi * Vidu' 0,075 g must xyclohexylamorni © Dimetyl sulfoxit Ia rat dc khi hit hoa tig xc hai, Cn str dung trong ti hit khéi, Vi de tinh 6 nén nha sn xuat khuyén c40 ding dung dich pha loang. © Phy thudc vo site dng cia thach 2. Chuan bj Hoa tan BCIG trong dimetyl sulfoxit hoc trong dung dich loZing theo khuyén cdo ctia nha san xuat Hoa tan tat ca cac thanh phén trén trong nuéc va dun dén séi. Chinh pH dé sau khi khit tring pH phai la 7,2 + 0,2 6 25°C, néu can. Khir tring 15 min 6 nhiét 46 121 °C trong néi hap ap luc (6.1). 5.2.2.3 Chuan bi cac dia thach Tién hanh theo 5.2.1.3. 4 dung cy thuy tinh Sit dung cac thiét bi, dung cy cia phang thit nghiém vi sinh théng thudng [xem TCVN 6404:2008 (iSO 7218:2007)] va cy thé nhu sau: 6.1. Thiét bj khu tring khé (ti) hoac khir tring u6t (néi hap ap luc). 6.2. Tu dm, c6 thé duy tri nhiét d6 37 °C + 1°C va 44°C + 14°C 6.3. TU say ho&c buéng say théng gid, cé thé duy tri nhigt do tir 25°C + 1 °C dén 50°C + 1°C, hoae tU théi khéng khi. 6.4. Ti lanh (dling dé bdo quan mdi truéng da chuan bi), 06 thé duy tri nhiét d6 63 °C + 2°C.:2008 6.5 Mang vo tring hodc khéng gay tc ché, béing xeniulo axetat hoac este hdn hgp clla xenlulo, c6 8 18 tir 0,45 um dén 1,2 um ho&c duéng kinh 85 mm, 6.6 Bé kep dau ti, v6 trling, dai khoang 12 cm, 6.7. May do pH, cé dé chinh xac dén + 0,1 dun vi pH Noung do t6i thiéu la 0,01 don vi pH. May do pH duge gan véi hé théing cn baing nhiét bang tay hoac tudong 6.8 Pipet xa hét (d4u théi), miéng rong va dung tich danh dinh 1 mi, duge chia vach 0,1 ml 6.9 Ong dong, cé dung tich thich hgp dé chudn bi méi truéng, 6.10 Binh, éng nghiém hodc chai, c6 dung tich thich hgp d8 khit tring va bao quan méi trudng cay. 6.11. Dia Petri, duang kinh khoang 90 mm. 6.12 Bd dan mau, bang thu tinh hoc chait déo, vi du: que bang thu tinh, duting kinh khong 3,5 mm, dai 20 cm, dutéc uén vung géc tai mét dau dai khoang 3 cm va dau cudi duye lam nh&n bang cach dét néng. 7 Lay mau ‘Diéu quan trong 1a mau gif dén phong thf nghiém phai la mau dai dién. Mau khong bi hu héng hoac thay dai thanh phan trong qua trinh van chuyén va bao quan. Viée lay mu khéng qui dinh trong tiéu chudn nay. Nén ly mau theo tiéu chun cu thé lién quan dén san phdm. Néu khéng c6 tigu chudn cy thé lién quan dén san pham thi cac ben tu thoa thuan vé van dé nay. 8 Chuan bj mau thir Chuan bi mu thir theo tiéu chudn cu thé lién quan dén san phdm. Néu khéng cé tiéu chuan cu thé lién quan dén san phdm thi cac bén ty thoa thuan vé van dé nay. 9 Cach tién hanh 9.1 Phan mu thit, huyén phi ban dau va cac dung dich pha loang tiép theo Xem TCVN 6507-1 (ISO 6887-1) hoac tiéu chudn cu thé lién quan dén sn pham can xac dinh.TCVN 7924-1:2008 9.2 Phuc héi 9.2.1 Ding bd kep dau th vé tring (6.6), d&t mang xenlulo axetat (6.5) lén bé mat khd cla hai dia thach MMGA (5.2.1.3), cht y (6.12) dé dan phang mang, néu can. khéng 68 l&n bot khi vao trong mang loc. Dung bd dan mau v6 tring Ding pipet vé tring (6.8), ly 1 ml mau thir ho&e huyén phii ban dau cho vao giifa mang, Ding bé dan mau v6 tring, dan déu dich cay trén khdp bé mat mang, tranh dé tran khéi mang. 9.2.2 L&p lai quy trinh trong 9.2.1 vdi cae dung dich pha long thap phan tiép theo, néu edn, dung pipet vo tring khac va que dan mau v6 tring khac cho mai dung dich pha long. 9.2.3 Bé cac dia da cay theo phudng nam ngang 4 nhiét dé phéng trong khoang 15 min cho dén khi dich cay 62 ngém sau vao trong thach. U dm cdc dia nay 4h + 1h trong td dm (6.2) & nhiét d6 37 °C ‘yi mang/mat thach hudng {én tn. 9.3 Chuyén sang méi trudng chon Ioc va & 3m 9.3.1 Sau khi phuc hdi, dling bd kep v6 triing (6.6), chuyén cac mang tif MMGA (mdi trudng phuc héi) sang céc dia thach dung méi truéng TBX (5.2.2.3). CANH BAO - Mang am ust sé dinh vao bé mat thach. Tranh dé ln bot khi. Khong sit dung dan mau, 9.3.2 U dm cac dia nay ti 18 h dén 24 h trong th dm (6.2) d nhiét G6 44 °C va khéng qua 45°C, véi mang/mat thach hudng lén trén, Khdng chéng cac dia qua ba lop. 9.4 Dém cac dan vj hinh thanh khudn lac (CFU) Sau giai doan & dm quy dinh (9.3.2), dém cdc CFU dién hinh cla Escherichia coli duong tinh b-glucuronidaza tran méi dia thach cht it hon 150 CFU dién hinh va it hon 300 CFU ting sé (dign hinh va khong dién hinh}. Néu dich cay khéng thé tach riéng va khéng thé quan sat duve cac khudn lac din hin! thi cde dia chita O CFU dign hinh cn duge xem xét theo c& phuong phap tinh khac nau iu 10 40 Bigu thi két qua 10.1 Yéu cdu chung Vige tinh toan trong 10.2 can tink cac trudng hop thuéng gap nhat khi tn nanh theo thyc hank phong the? gm tot. Cling cd mét sd trutng hep dae biét nhung cling hiém khi xy ra (vi dy nh 85TCVN 7924-1:2008 long CFU rat khae nhau giita hai dia tu cling mét dung dich pha loang, hodc cac ty Ié khdc nhau nhiéu tur mét hé sé pha long gilla cac dia tU hai dé pha loang lién tiép). Cac két qua dém can duge ngudi phn tich cé nang luc kiém tra, gidi thich va két qua dé ciing c6 thé bi loai bd. 10.2. Tinh toan Dé c6 két qua diing, cn dém cdc CFU dién hinh trén it nhat mot dia chifa t6i thiu 15 CFU mau xanh dién hinh. Tinh N, 86 lugng CFU ctia Escherichia coli dang tinh B-glucuronidaza c6 mat trong mau this trén mililit hodc trén gam sn phém bang céng thite (1): Ye Vin, +0.1n,)ad m trong d6 Za_ la téng cdc khudn lac dém dude trén tat cd cac dia duge giftlai sau hai 46 pha lodng lién tiép, cé it nhat mét dia chifa tdi thigu 18 CFU mau xanh; n, la s6 dia dude gitt lai tai dé pha loding thi nat; V. thé tich dich cdly da ding trén méi dia, tinh bang milli; n, ta 86 dia duge gilt lai tai dd pha loding thi hai, d—_hé 6 pha loding tuong ing vai d6 pha lodng thir nhat dude gif lai [d = 1 trong trudng hop (cac mau 6 dang léng) mau thi duge cay truc tiép}. Lam tron cac két qua dén hai chit s6 c6 nghia {xem TCVN 6404:2008 (ISO 7218:2007)]. Lay két qua la sé lugng Escherichia coli duong tinh §-glucuronidaza trén mililit (san pham dang iéng) hode trén gam (sn phdm dang khac) dugc biéu thi theo s6 nguyén dén hai chit sé c6 nghia (dui 100) hoac theo s6 tir 1,0 dén 9,9 nha luy thite cia 10. 10.3. Udc tinh cde sé lugng nho 10.3.1 Néu cé hai dia (ca mau thit (sin phdm dang Idng) hoac otia huyén phil ban dau (san phdm dang khac) hoac cla 46 pha long thi nhat duce cay hoac duge gif lai] chia it hon 15 CFU diéa hinh, thi tinh Ne sé CFU ctla Escherichia coli duang tinh B-glucuronidaza cé mal trong mau thir la trung binh cla hai dia song song theo céng thilc (2) (2) 1TCVN 7924-1:2008 trong do Dic la téng cdc CFU dién hinh dém duge tren hai dia: V thé tich dich ey da ding trén méi dia, tinh bang miflit; 1 la.s6 dia duge git lai (trong truding hdp nay n = 2); d_-hé 86 pha lodng tudng Ung vai dé pha loang thif nhat dude git lai [d = 1 trong truéng hop (cac mau 4 dang lng) khi mau thif dude cdy truc tiép]. Lam tron cc két qua dén hai chil sé cd nghia [xem TCVN 6404:2008 (ISO 7218:2007)]. Biéu thi két qua nhu sau: ~ 86 lung Escherichia coli duong tinh B-glucuronidaza u6c tinh trong mét mililit (sn phdm dang léng) hoac trong mét gam (san phdm dang khac): Ne = Y- 40.3.2 Néu hai dia [cia mau thi (san phdm dang léng) hoac huyén phu ban d4u (san phdm dang khac) hoac tir 4 pha long thi nhat da cy hoac gitt lai] khong chita CFU dign hinh nao, thi bidu thi két qua nhu sau: ~ithon td Escherichia coli duang tinh B-glucuronidaza trong milit (s8n ph&m dang Ing) hoae trong gam (sn pham dang khac); trong d6 d la hé sé pha lodng cila huyén phil ban ddu hoac dé pha loang this nhat da cdy ho’c git lai {d= 1 khi st dung san phdm dang !éng (mau thi) khong can pha lodng] 40.3.3 Néu t8t ca cac CFU dién hinh hoa khéng dién hinh trén hai dia & d6 pha loding thif nhat d, chifa nhiéu hdn 300 CFU mau xanh cé thé nhin thay, hoadc néu trén hai dia 6 dé pha loang tiép theo d, chifa it hon 300 khuan lac, khong c6 CFU dién hinh nao can dém, thi biéu thi ét qua nbu sau - it hon t/d; va nhigu hen Vd, Escherichia coli dudng tinh B-glucurcnidaza cé trong mét millit (sn phm dang léng) hodc trong mét gam (san phdm dang khac); trong dé d, va d, 1a cdc hé sé pha loang tuang ting vai c&c dung dich pha loang d, va d-. 10.3.4 Néu tat cd cac CFU dién hinh ho&e khéng dién hinh trén hai dia & 43 pha lodng thir nhat 0, ‘chita nhiu hon 300, khéng cd CFU dign hinh cé thé nhin thay, ho&e néu trén hai dia 4 43 pha loang Lip theo d, chia it han 300 khudn lac, khong c6 CFU dién hinh can dém, thi bidu thi két qua nhu sau: ~ ft hon 1/d, cac CFU cla Escherichia coli duong tinh B-glucurenidaza ¢6 trong mét miliit (san phd dang léng) hoac trong mét gam (sn phdm dang khac); 12TCVN 7924-1:2008 trong dé d, la cdc hé sé pha loang luting img vdi dung dich pha loang d, 10.4. Phuong phap tinh toan: Cac truéng hgp dic biét 10.4.1 Trong truéng hyp, trén hai dia 4 dd pha long d, chifa ln hon 150 CFU dién hinh, trén hai dia & 46 pha long tiép theo d; chifa it hon 15 CFU din hinh: ~ néu s6 lugng CFU dién hinh trén tng dia & 46 pha long d, n&m trong khoang ttr 150 dén 167 (gidi han trén cla khodng tin cy déi véi gid tri trung binh b&ng 150 CFU), thi st! dung phuong phap tinh déi vdi truding hdp chung (10.2); ~ néu sé lung CFU dién hinh trén timg dia 6 46 pha loang d, nhiéu hon 167 (gidi han trén cia knoang tin cay di véi gia tri trung binh bang 150 CFU), thi chi tinh dén két qua cla s6 dém cila dé pha loang d, va thuc hién tiép vdi viéc dém sé ludng nhéd (10.3). 10.4.2 Trong trutng hdp, sé dém cac CFU dién hinh trén méi dia déi véi tat c& cdc 3 pha long 43 duoc cay c6 nhiéu hon 150, thi biéu thi két qua nhu sau: ~ nhiéu hon 150/d Escherichia coli duténg tinh B-glucuronidaza c6 trong miliit (san pham dang |dng) hoac gam (san pham dang khac). trong d6 d a hé sé pha loang clla dung dich pha loang da cay sau cing. 10.4.3 Trong truéng hgp chi c6 hai dia tir dé pha long thap nhat (néng 46 cao nhat) chéa it hn 150 CFU dién hinh, thi tinh 86 N’ cae Escherichia coli dudng tinh B-glucuronidaza cé mat trong mau thit la trung binh sé khuan lac dém dugc trén hai da, stt dung céng this (3): (3) trong 46 De ta téng sé khudn lac dién hinh dém duge trén hai dia, c6 it nhat mét dia chia ti thigu la 15 CFU ign hinh; V la thé tich dich cay da cay trén méi dia, tinh bang millt; nt la 86 dia duge git! lai (trong truéng hp nay n = 2); dla hé sé pha long tudng tg véi d6 pha loding duge git lai Lam tron két qua dén hai chiy sé c6 nghia [xem TCVN 6404:2008 (ISO 7218:2007)] 13TCVN 7924-1:2008 10.5 Cac gidi han tin cay Xem TCVN 6404:2008 (ISO 7218:2007). 11 Bao cdo thir nghigm Bao cdo thir nghiém phai néu ré: moi thdng tin can thiét vé nhan biét day du vé mau thts, — phuong phap lay mau da st dung, néu biét; — phugng phap thir da sir dung, vin dan tigu chun nay; ~ talc cc diéu kién thao t4c khéng qui dinh trong tiéu chuzin nay, ho’c duge xem la tuy y, cling vai moi tinh hudng bat thuting c6 thé anh hung dén két qua. két qua thi nghiém thu duge, néu 13 phudng php bigu thi da sit dung; va néu dap ing duoc yéu cau vé dé lap lai thi néu két qua cuéi cling thu dutic. 14Ol (2) 8) (41 (5) (6) (7 (8 {3} (10) TCVN 7924-1:2008 Thu muc tai ligu tham khao BLAZKO N. Evaluation of the i-glucuronitiase substrale 5-bromo-4-chtoro-3-indoly-B-D glucuronide in a 24 hour direct plating method fot Eseherichts-cab J. Food Protection, 51. p. 402 Dauare J.M.. CAMP8ELL D.R and JOHNSON R.W Simplified direc? plating method for enhanced recovery of Escherichia coli m food. Journal of Food Science 50. 1986, pp 1736-1737. 1746. DEuIse G.L. and Ley A. Rapid detection of Escherichia coli in urine samples by a new chromogenic -glucuronidase assay. J. Clin. Microbiol, 27. 1969, pp. 778-779. KiulAN M. and BULOW P. Rapid diagnosis of Enterobacieriaceae. Detection of bacterial glycosidases. Ada Pathol, Microbiol. Scand.. Sect. B. 84, 1976. pp. 245-251 KiuiAN M. and BuLow P. Rapid identification of Enterobacteriaceae. Use of a -glucuronidase detecting agar medium (PGUA agar) for the identification of Escherichia coli in primary cultures of urine samples. Acta Pathol. Microbiol. Scand., Sect, B. 87, 1979. pp. 271-276 Ley AN. Bowers RJ. and WOLFE S. Indoxyl-(}-D-glucuronide, a novel chromogenic reagen! for the specific detection and enumeration of Escherichia coli in environmental sample. Canadien Journal of Microbiology, 34, 1988. pp. 690-693. MANAFI M. and KNEIFEL W. A combined chromogenic-fluorogenic medium for the simulianeous detection of total coliforms and E. coli in water. Zentralbl. Hyg.. 189, 1989, pp. 225-234. OGOeN L.D. and WATT A.J. An evaluation of fluorogenic and chromogenic assays for the direct enumeration of Escherichia coli. Letters in Applied Microbiology. 13, 1991, pp. 212-215. RESTAINO L., FRAMPTON E.W. and LYON R.H. Use of chromogenic substrate 5-bromo-4-chloro-3- indotyl- beef. J Food Protection. 53 (6). 1990. pp. 508-510 D-glucuronide (X-GLUC) for enumeration of Escherichia coli on 24 hours from ground WATKINS W.O., RIPPEY S.C., CLAVET C.R., KELLY-REITZ Dw, and BURKHARDT W. Novel compound for identifying Escherichia coli. Applied Environmental Microbiology. 54. 1988, pp. 1874-1875 15