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Skin Safety Evaluation of Long-Term treatment of an Electrolyzed Neutral

Oxidizing Water on C57BL/6 mice

Article · March 2021

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Md. Habibur Rahman Ailyn Fadriquela

Yonsei University Yonsei University Wonju College of Medicine, Wonju City, South Korea
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Yonsei University
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 한국물학회지
Vol.8 No.1

ISSN : 2233-4653(Print)

Skin Safety Evaluation of Long-Term treatment of an Electrolyzed Neutral

Oxidizing Water on C57BL/6 mice

Md. Habibur Rahman, Ailyn Fadriquela, Johny Bajgai, Seong-Hoon Goh, Cheol-Su Kim, Kyu-Jae Lee

To cite this article : Md. Habibur Rahman, Ailyn Fadriquela, Johny Bajgai, Seong-Hoon Goh, Cheol-Su Kim, Kyu-Jae Lee (2020) Skin
Safety Evaluation of Long-Term treatment of an Electrolyzed Neutral Oxidizing Water on C57BL/6 mice , 한국물학회지, 8:1, 52

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 52

Skin Safety Evaluation of Long-Term treatment of an Electrolyzed

Neutral Oxidizing Water on C57BL/6 mice

Md. Habibur Rahman1,2, Ailyn Fadriquela1, Johny Bajgai1, Seong-Hoon Goh1,

Cheol-Su Kim1, Kyu-Jae Lee1,*

1
Department of Environmental Medical Biology, Wonju College of Medicine Yonsei University,
2
Global Medical Science, Graduate School of Yonsei University, Wonju, Gangwon, 26426, Republic of
Republic of Korea
[Provider:earticle] Download by IP 165.132.230.201 at Thursday, March 11, 2021 10:10 AM

Skin is a complex barrier organ that can fight against various endogenous and exogenous
factors. The damaged skin barrier can lead to different skin problems that can exhibit the skin
condition such as a decrease of elasticity and sebum level, an increase of water loss,
strengthening of keratinocyte cohesion and wrinkle formation, which will eventually lead to
different skin diseases. With the current use of chlorine-rich water as disinfectant and its
potential in medical field, it is important to check its safety use. The objective of the study was
to evaluate skin safety of electrolyzed neutral oxidizing water (ENOW) (pH: 7.11, oxidation
reduction potential: 450 mV, available chlorine concentration: 5.0 ppm) after the long-term use
(90-day skin application) on the skin of C57BL/6 mice compared to normal control group (NC)
treated with tap water (TW) (pH: 7.8, oxidation reduction potential: 500mV, available chlorine
concentration: 0.7 ppm). We were measured skin state such as the moisture, elasticity, sebum,
pore size, and wrinkle. Oxidative stress markers such as reactive oxygen species (ROS), nitric
oxide (NO), glutathione peroxides (GPx) and catalase (CAT) were measuredin using serum and
skin lysate samples. Inflammation cytokines such as IL-1α, IL-1β, IL-13, GM-CSF and TNF-α, and
the Th1/Th2 cytokines such as IL-2, www.earticle.net
IL-4, IL-5, IL-6, IL-10 and IL-12 were also analyzed. In
consequently, The skin state of ENOW group showed higher level of elasticity and moisture
compared to NC group. ROS and NO levels in skin did not show adverse effect in ENOW group.
GPx were also controlled in all groups, however, a significant decrease in CAT level was
observed in both NC and ENOW groups. Moreover, serum inflammatory cytokine of ENOW group
did not show significant difference as compared to NC and TW groups, however, GM-CSF
showed a significantly lower level in the ENOW group than NC group. Cytokines related to
allergic response showed no difference in the skin. Taken together, long term treatment of
ENOW might be safe on skin in vivo. However, more clinical studies are needed to verify the
skin safety of ENOW.

Keywords: Electrolyzed neutral sterilizing water, Skin safety, Long-term treatment, Anti-oxidants, Anti-inflammation,
anti-allergy

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