ACT 13

2. Will there be a possibility that hemolyzed serum can be use in this test? 

In vitro hemolysis is known to negatively interfere with sodium due to a diluting effect, as the
intracellular concentration of sodium is significantly lower than the concentration in serum or plasma.

Hemolyzed serum or plasma is unsuitable for several chemistry determinations because substances
usually present within cells (e.g., K+) can be released into the serum or plasma if serum is left on the
cells for a prolonged period. In addition, several other constituents, including the enzymes acid
phosphatase, lactate dehydrogenase (LDH), and aspartate aminotransferase (AST, GOT), are present in
large amounts in RBCs, so hemolysis of red cells will significantly elevate the value obtained for these
substances in serum. Hemoglobin is released during hemolysis and may directly interfere with a
reaction, or its color may interfere with photometric analysis of the specimen. The procedure to be done
should always be checked to determine whether abnormal-looking specimens can be used.

Turgeon, M. L., Linné, J. J., & Ringsrud, K. M. (2012). Linne & Ringsrud's clinical laboratory science: The
basics and routine techniques (6th ed.). Maryland Heights, MO: Elsevier/Mosby.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416812/#:~:text=As%20previously%20reported%2C%20in
%20vitro,concentration%20in%20serum%20or%20plasma