Products For Microscopy 05018

Products for Microscopy
The Color of Life Science
The life science business of Merck operates as

MilliporeSigma in the U.S. and Canada.
A
For over
100 years,
dyes and solutions of dyes have been part of the Merck
product range. This tradition and experience have made
Merck one of the world’s leading suppliers of microscopy
products. The products for microscopy, a comprehensive
range for classical hematology, cytology, histology and
bacteriology, are constantly being expanded and adapted
to the needs of the user and to comply with all relevant
environmental regulations. The “Neo” product range e.g.
is both user-friendly and environmentally compatible. As
always, the most important aspect is quality, which
ensures that reproducible results can always be
obtained. The majority of the microscopy products
featured in this brochure, belong to the group of in vitro
diagnostic (IVD) medical devices and comply with the EU
IVD Directive and bear the CE logo on the label.
However, some of the products listed in this brochure
are not-IVD materials but are required to facilitate the
staining procedures. CE stands for Community of
Europe. All CE marked IVDs are produced according to
quality management ISO 13485, which documents all
steps of the product lifecycle.
Insert sheets for all CE registered products are

available on the internet at:
www.merckmillipore.com/microscopy
Quality control is hence of the utmost

importance in the manufacturing process. All
Merck products are manufactured using raw
materials and solvents that meet the most
stringent quality criteria.
Prior to releasing the products for particular

applications, all relevant chemical and physical
parameters are carefully tested. The methods
used for testing comply with international
standards and are updated whenever required.
he performance of each product is also verified

T
using a suitable application; the product is
released for sale only after the relevant
specifications are met.
The excellent reproducibility of the results for

which Merck’s microscopy products are
renowned is a result of our advanced and
meticulous quality control.
B
Table of Contents
1 Universal Reagents
for Microscopy 6 Products for Histology
36
36-37
Staining Solutions for Histology
Staining Kits for Histology
4 Fixing Media
38-39 ISOSLIDE™ Control Slides
6 Decalcifer
for Histology
7 M-FREEZE™ Cryo-Embedding Medium
40-41 Other Products and Auxiliaries
7-8 Mounting Media
for Histology
8 Immersion Media
7
9-13 Other Products and Auxiliaries
for Microscopy
Neo-Products
2 Dyes for Microscopy

15-16 Certistain® Dry Dyes for Microscopy
43
44
Neo-Clear™ Xylene Substitute and
Neo-Mount™ Mounting Medium
Neo-Cytocolor™ Rapid Staining Kit
8
17-18 Other Dry Dyes and Dye Mixtures
19 Fluorescence Dyes
Immunodetection
3 Products for
Bacteriology
Reagents and
Instrument for IHC
46 SNAP id® 2.0 IHC
21 Staining Kits for Bacteriology 47 Biochemical Reagents
22-23 Staining of Mycobacteria
9
23 Staining Solutions for Bacteriology
24 Other Products and Auxiliaries
for Bacteriology Appendix
4
49-51 pH-indicators for Titrations in
Aqueous Media
Products for Cytology 51
52
General Mixture-Rules
Buffer Solutions
26-27 Staining Solutions for Cytology 53 Cleaning Agents for
27 Other Staining Solutions Laboratory Equipment
28 Other Products and Auxiliaries 54 References
for Cytology 55-57 Classified Register
5 Products for
Hematology
30 Staining Kits and Solutions
for Hematology
30 Other Staining Solutions
31 Dye Mixtures for Hematological
Staining Solutions
32-33 Cytochemical Reagents
33 Reagents for Manual Blood
Cell Counting
34 Other Products and Auxiliaries
for Hematology
CE Certification of IVDs
The European In Vitro Medical Devices Directive (98/79/
EC) of 27 October 1998 is the legislative instrument on
which local IVD legislation within the EU Member States
is based. It has been mandatory since December 2003
for all In Vitro Medical Devices (IVDs) that are placed on
the market in the EU by manufacturers to be CE certified
and bear the CE marking on product labels, instructions
for use and sales packaging. All lVDs placed on the
market by suppliers and used as IVDs must bear the CE
marking from December 2005.
The term In Vitro Diagnostic Devices means any medical

device which is a reagent, reagent product, calibrator,
control material, kit, instrument, apparatus, equipment
or system, whether used alone or in combination,
intended by the manufacturer to be used in vitro for the
examination of specimens, including blood and tissue
donations, derived from the human body, solely or
principally for the purpose of providing information:
• concerning a physiological or pathological state,

• concerning a congenital abnormality,
• to determine the safety and compatibility with
potential recipients,
• to monitor therapeutic measures
Classification rules of IVD directive:
Other/General Device:
all devices except Annex II and self-testing devices.
Device for Self-Testing (not listed in Annex II):
a device intended by the manufacturer to be able to be
used by lay persons in a home environment, excluding
self-test devices covered in Annex II.
Device of List B, Annex II of the Directive:
which, amongst others, includes reagents and products
for rubella, toxoplasmosis and phenylketonuria as well as
devices for self testing for blood sugar.
Device of List A, Annex II of the Directive:
which includes reagents and products for human
immunodeficiency virus I and II, hepatitis B, C and D.
CE certification is a self-certification process in which

product details are submitted according to the valid
EDMA (European Diagnostic Manufacturers Association)
code. Merck successfully completed the CE certification
process in December 2003 for all Products for
Microscopy. From that time until the present, all IVDs
belong to the low-risk group and are exempted from
Annex II of the IVD Directive and are not self-testing
devices. New products are developed, manufactured,
quality-tested and documented in accordance with the
IVD regulations.
Universal Reagents
for Microscopy
1 3
Universal Reagents for Microscopy
Fixing Media
Fixing is a procedure designed to stop all the processes in a cell whilst maintaining, the structure and integrity of
the tissue and at the same time effectively preventing processes such as autolysis, decomposition and putrefaction.
The fixing agent is selected according to the diagnostic problem being addressed, the type and size of the available
material and the required embedding and staining methods to be used.
Product Size Catalog No. Directions for use
Acetic acid (glacial), 100% 1 L, 2.5 L 100063 1–4% in conjunction with other fixatives
Acetic acid (glacial) 100% 1 L, 2.5 L 100056 1–4% in conjunction with other fixatives
Acetone 1 L, 2.5 L 100014 In conjunction with other fixatives
Acetone, extra pure, Ph, Eur, BP, NF 1 L, 2.5 L 100013 In conjunction with other fixatives
Chromium(IV) oxide, GR, ACS 250 g 100229 0.5–0.25% aqueous solution
absolute for analysis EMSURE® ACS, ISO, Reag. Ph Eur

Ethanol 1 L, 2.5 L 100983
Fixing time 2–4 hours for a thickness of max. 5 mm
absolute suitable for use as excipient EMPROVE® exp Ph
Ethanol 1 L, 2.5 L 100986 Eur, BP, JP, USP
denatured with about 1% methyl ethyl ketone for analysis
Ethanol 1 L, 2.5 L 100974 EMSURE®
Formaldehyde solution 4%, buffered, (pH 6.9) 5 L, 10 L 100496 Ready-to-use fixing solution
Contains calcium carbonate as acid binding basis;

Formaldehyde solution min. 37%, freeform acid 1 L, 2.5 L 103999
filter before use
1 L, 2.5 L,
Formaldehyde solution about 37% 104003 GR for analysis stabilized with about 10% methanol
5 L, 25 L
Store in a cool place protected from light,
Glutardialdehyde solution 250 mL, 1 L,
104239 filter before use if a sediment forms,
25% for electron microscopy 2.5 L
with 0.1 M phosphate buffer pH 7.42
Glutardialdehyde solution 25% 25 mL, 100 mL 112179 Electron microscopy, purified and filled under nitrogen
Fast staining with Hemacolor® rapid staining kitt for blood

Hemacolor® Rapid Staining Kit 2.5 L 111955
and bone marrow
Fixation of blood and bone marrow smears using the
LEUCOGNOST™ Fixing Mixture 500 mL 112327
various LEUCOGNOST™ leukemia detection kits.
Methanol 1 L, 2.5 L 106009 Fixation of blood smears
Intestine, paraffin section,

Alcian blue-nuclear fast red staining
4
M-FIX™ Spray Fixative
This is suitable for cytological smears that have to be stained using classical or modified Papanicolaou staining
techniques and which must be fixed immediately whilst still moist in order to prevent the cells from drying out.
Smears can be fixed in alcoholic solution for 30 minutes or, better, using M-FIX® spray fixative. M-FIX® spray fixative
contains polyethylene glycol in aqueous/alcoholic solution; this effectively prevents the cells from drying out and also
protects them during transport. M-FIX® spray fixative is suitable for fixing gynecological specimens but also for non-
gynecological materials such as sputum, urine sediment, effusions, lavages, FNAB etc.
1 pack
M-FIX® Spray Fixative 103981 Pump spray mechanism fixes about 250 preparations
100 ml, 1 L (refill)
Saturated aqueous stock solution:
Mercury(II) chloride (sublimate), GR, ISO, ACS 50 g, 250 g, 1 kg 104419
dissolve 60 g in 1,000 ml of hot distilled water
Nitric acid min. 65% (about 1.40), GR 1 L, 2.5 L 100456 Visualizing neurofibrils, 3–7% solution
Nitric acid min. 65%, pure, DAB 1 L, 2.5 L 100443 Visualizing neurofibrils, 3–7% solution
1–2% aqueous solution, several hours necessary to dissolve

100 mg, 500 mg,
Osmium(VIII) oxide 124505 the crystals, use only redistilled water for preparing the
1 g (ampoules)
solution, a black-colored solution is unusable
Osmic acid solution 2% 5 ml (ampoule) 109266 Yellowish-brown coloration has no effect on usability
Platinum(II) chloride (73% Pt), for synthesis 500 mg 824566 Aqueous solution 1:300 for fixing mitochondria
Potassium dichromate, GR, ACS, ISO 500 g, 1 kg 104864 Fixing lipids and mitochondria 3% aqueous solution
1-Propanol for analysis EMSURE ACS,

®
1 L, 2.5 L 100997
ISO Reag. Ph Eur
1-Propanol, extra pure 1 L, 2.5 L 100996
2-Propanol (iso-propyl alcohol) for analysis

1 L, 2.5 L 109634 Instead of ethanol (Papanicolaou staining)
EMSURE® ACS, ISO Reag. Ph Eur
2-Propanol (iso-propyl alcohol) extra pure, USP 1 L, 2.5 L 100995
Trichloroacetic acid, GR, ACS 100 g, 250 g 100807 5–10% solution
Tungstophosphoric acid for analysis EMSURE® 100 g, 250 g 100583 Visualizing connective tissue, fibrin and bones 1–5%
5
Decalcifier
OSTEOMOLL® and OSTEOSOFT® Decalcifier-Solutions
Decalcification methods are necessary for optical microscopic examinations of bone and other hard tissue in routine
histological procedures. The material to be decalcified is placed in an excess of decalcifying solution and demineralized
(decalcified) in this. The decalcification time is dependent on the size and structural density of the hard tissue, while
the composition of the decalcifying solution also exerts a decisive influence on the process. Decalcification of bone
and hard tissue requires the use of either inorganic acids, as is the case with OSTEOMOLL® decalcifier-solution, which
liberates the acids of the mineral salts and can subsequently be rinsed out. The decalcifying solution has been stained
to facilitate the identification of the decalcifying procedure – OSTEOMOLL® decalcifier-solution is blue. The dye used
is inert in terms of any effect on the tissue to be decalcified. When decalcifying sensitive, calcium-containing tissue
a solution such as OSTEOSOFT® decalcifier-solution is used, which contains complex- or chelate-forming agents that
bind the calcium ions of the tissue. This type of decalcifying solution preserves the antigen structures in the tissue,
with the result that immunological procedures can be conducted. The decalcifying solution has been stained to
facilitate the identification of the decalcifying procedure – OSTEOSOFT® decalcifier-solution is yellow. The dye used is
inert in terms of any effect on the tissue to be decalcified.
OSTEOMOLL® Decalcifier-Solution 1 L, .5 L 101736 Bone-decalcification solution for bone and hard tissues in histology
OSTEOSOFT® Decalcifier-Solution 1 L, 10 L 101728 Bone-decalcification solution for sensitive, calcium-containing tissue in histology
OSTEOSOFT® OSTEOMOLL®
Decalcifier-Solution Decalcifier-Solution
Bone marrow, paraffin section,

OSTEOSOFT® decalcifier-solution,
immunohistochemistry
6
Embedding Media
In order to achieve uniform sectioning quality, defined hardness and homogeneity of the material to be processed is
necessary. This is achieved by the use of embedding agents which are harder and denser than necessary. Traditional
embedding agents are paraffins, synthetic, resins and gelatine.
Histosec™ Paraffin Pastilles

Histosec™ paraffin pastilles products are selected paraffins with added polymers and which are available with and
without DMSO. Specially selected raw materials and standardised quality guarantee complete penetration of the
tissue being processed and allow significantly shorter processing times to be used in a histoprocessor. The increased
elasticity of embedded tissue enables excellent individual and series sections to be prepared.
1 kg, 4 x 2.5 kg, 25 kg

Histosec™ Paraffin Pastilles 111609 Paraffin sections, contains DMSO
pastilles
4 x 2.5 kg, 25 kg
Histosec™ Paraffin Pastilles without DMSO 115161 Paraffin sections
pastilles
Histosec™ Paraffin Pastilles 60 4 x 2.5 kg
101676 Paraffin sections
solidification point 58–60 °C pastilles
4 x 2.5 kg
Paraffin Pastilles 107164 Pure paraffin specially for histology
pastilles
M-FREEZE™ Cryo-Embedding Medium

The product M-FREEZE™ cryo-embeding medium for microscopy is used in the routine preparation of unfixed tissue
(native material) of human origin for frozen-section analysis. It is also used for preparing cryo sections for enzyme
cytochemistry and immunohistochemistry methods to avoid fixation artefacts.
M-FREEZE™ cryo-embedding medium (NEW) 100 mL 103693 Embedding medium for frozen sectioning.
Mounting Media
For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a
suitable mounting agent and covered with a cover glass. Mounting agents can be aqueous and non-aqueous; the type
used depends on the protocol involved. In the case of non-aqueous agents, the preparations must be fully dehydrated.
One of the most important parameters of mounting agents is the refractive index (nD); this should be around 1.5, the
refractive index of glass.
Aqueous Mounting Media
Aqueous mounting agent, used for LEUCOGNOST™ leukemia detection kit

Aquatex® Mounting Medium 50 mL 108562
stained specimens
Gelatine 500 g 104070
Glycerol (ref. index nD20°1.47) 250 mL 104095 Aqueous preparations and preparations for fluorescence microscopy
Kaiser’s glycerol gelatine 100 g 109242 Preparations, histological sections for substance or enzyme determination
Kaiser’s glycerol gelatine, phenol-free (NEW) 100 g 108635 Preparations, histological sections for substance or enzyme determination
A few crystals of thymol are added to avoid fungal contamination; nuclear

Sorbitol F liquid 50 L 102993 stain retained following hematoxylin stain; not suitable for Azan and van
Gieson staining
7
Non-aqueous Mounting Media
Canada balsam 25 mL, 100 mL 101691 Natural resin
DPX new 500 mL 100579 Non-aqueous mounting agent
Entellan™ Mounting Medium 500 mL 107960 Permanent slides (contains toluene)
Rapid mounting agent, permanent slides, no bubble

Entellan™ new Mounting Medium 100 mL, 500 mL, 1 L 107961
formation at high ambient temperatures (contains xylene)
Entellan™ new for cover slipper 500 mL 100869 Permanent slides, for use with cover slipper
Mounting agent for the even coating of cytological smears

M-GLAS® Glass Coverslip 500 mL 103973
instead of cover slips (contains toluene)
100 mL (dropper bottle),
Neo-Mount™ Mounting Medium 109016 After Neo-Clear™Xylene Substitute use
500 mL
Paraffin liquid 100 mL, 500 mL 107161
Immersion Media
Immersion media are used in conjunction with immersion slides and are located between the surface of the specimen
and the lens of the microscope. Immersion media are liquids that are frequently of an oily nature and which have a
defined refractive index. It is important that the refractive index (nD) is about 1.5, the figure for glass. This enables a
homogeneous oil immersion to be achieved.
Product Size Catalog No. Refractive index nD20°
Acetonitrile for analysis EMSURE ACS, ISO, Reag. Ph Eur

®
1 L, 2.5 L 100003 1.345
Acetonitrile, extra pure 1 L, 2.5 L 115500 1.345
1-Bromonaphthalene, for synthesis 100 mL, 500 mL, 2.5 L 806210 1.658
Cinnamaldehyde 5 mL, 250 mL, 1 L 802505 1.622
1,2-Dibromoethane, for synthesis 5 mL, 250 mL, 1 L 800952 1.5368
Diiodomethane 100 mL 106053 1.740
Dinonylphthalate 100 mL 109669 1.486
1,2-Ethanedithiol, for synthesis 100 mL 800795 1.125
Ethylene glycol, extra pure 1 L, 2.5 L 100949 1.432
Ethylene glycol for analysis EMSURE® Reag. Ph Eur 1 L, 2.5 L 109621 1.432
Immersion oil (viscosity about 100–120 mPa·s) 100 mL, 500 mL 104699 1.516
Immersion oil, acc. to ISO 8036, Halogene-Free (NEW) 100 mL 103699 1.518
8
Other Products and Auxiliaries for Microscopy
Acetic acid, glacial, 100%,

1 L, 2.5 L 100056 Preparing stain solutions
extra pure Ph Eur, BP, JP, USP, E 260
Acetic acid (glacial) 100% 1 L, 2.5 L 100063 Preparing stain solutions
Acetone, extra pure, Ph, Eur, BP, NF 1 L, 2.5 L 100013 In conjunction with other fixatives, as intermedium
Acetone 1 L, 2.5 L 100014 30–, 60–, 90–,100%, duration 15–20 minutes
Albumin (from bovine blood) 25 g, 100 g 112018 Mounting histological sections albumin-glycerol mixture 1:1
Aluminum chloride hexahydrate, extra pure,

1 kg 101084 Addition to stain solutions (paracarmin acc. to Mayer)
Ph Eur, BP, USP
Aluminum sulfate 18-hydrate, extra pure,
5 kg 101102
Ph Eur, BP
Ammonia solution, 25%, extra pure 1 L, 2.5 L 105422
Ammonia solution,min. 25%, GR 1 L, 2.5 L 105432 Addition to stain solutions (carmine solution acc. to Best)
Ammonium aluminum sulfate12-hydrate

500 g 101031
for analysis EMSURE® (ammonium alum)
Ammonium iron(III) sulfate 12-hydrate
500 g 103776 Iron alum solutions
(iron alum), GR, ACS, ISO
Aniline 250 mL, 1 L 101261 Clearing agent
Preparing barbital buffers in the detection of alkaline

Barbital sodium (buffer substance)
leukocyte phosphatase
Benzyl benzoate, Ph Eur, BP, USP 25 L 101806
Boric acid, GR, ACS, ISO, Reag. Ph Eur 100 g, 500 g 100165 Addition to stain solutions (Manson and Schwarz stain)
1 pack Preparing buffer solution pH 6.4 according to Weise for

Buffer tablets pH 6.4 111373
100 tablets blood smear staining
n-Butyl acetate, extra pure 2.5 L 101974 Instead of toluene, xylene
n-Butyl acetate for analysis EMSURE® 1L 109652 Instead of toluene, xylene
Calcium chloride dihydrate, GR, ACS, Reag. Ph Eur 250 g, 500 g, 1 kg 102382 Addition to stain solutions
Carbon disulfide, extra pure 1L 102211 Intermedium
Carbon disulfide, ACS, Reag. Ph Eur 1L 102214 Intermedium
Carbon tetrachloride for analysis EMSURE® 1 L, 2.5 L 102222
Chloroform, extra pure, DAB 9 1 L, 2.5 L 102431
Chloroform for analysis EMSURE® ACS,

250 mL, 1 L, 2.5 L 102445
ISO, Reag. Ph Eur
Chromium(VI) oxide, GR, ACS 250 g 100229
Citric acid anhydrous powder, suitable for use as

excipient EMPROVE® exp Ph Eur, BP, JP, USP, 5 kg 100241
E 330, FCC
9
5,5-Diethylbarbituric acid sodium salt Preparing barbital buffers in the detection of alkaline
100 g, 500 g 106318
(sodium barbital) leukocyte phosphatase
Diethyl ether, extra pure 1 L, 5 L 100923
Diethyl ether stabilized with

2,6-di-tertbutyl-4-methylphenol (5 mg/l) 1 L, 5 L 100921
for analysis EMSURE® ACS, ISO, Reag. Ph Eur
2,2-Dimethoxypropane for synthesis 500 mL, 1 L 802936
Dioxan, extra pure 1 L, 2.5 L 103115
Dioxan for analysis EMSURE®

1 L, 2.5 L 109671
ACS, ISO, Reag. Ph Eur
(ethyl alcohol) absolute for analysis EMSURE® ACS,
Ethanol 1 L, 2.5 L 100983 ISO, Reag. Ph Eur
Ascending alcohol series
Ethanol 1 L, 2.5 L 100986 (ethyl alcohol) absolute, pure, Ph Eur, BP, USP
denatured with about 1% methyl ethyl ketone for

Ethanol 1 L, 5 L 100974 analysis EMSURE®
Ascending alcohol series
Ethylene glycol monomethyl-ether
1 L, 2.5 L 100859 Clearing agent
for analysis EMSURE® ACS, Reag. Ph Eur
Gelatine for microbiology 100 g, 500 g 104070 Bloom-number about 200
Gelatine, powder 500 g, 1 kg 104078 pH value 5–6
Glycerol (about 87%), extra pure, Ph Eur, BP 1 L, 2.5 L 104091 Clearing agent
Glycerol (about 87%) for analysis EMSURE ®

500 mL, 1 L, 2.5 L 104094 Binder
ACS, Reag. Ph Eur
Glycerol 250 mL 104095 Specially tested for fluorescence microscopy
Gold trichloride acid trihydrate, yellow

1 g, 5 g 101582 Gold impregnation: 1% solution
1N Hydrochloric acid 1L 109057
Hydrochloric acid 2 mol/l 1L 109063
Hydrochloric acid 25%, extra pure, Ph Helv 2.5 L 100312
Hydrochloric acid min. 25% (about 1.125)

1 L, 2.5 L 100316
Hydrochloric acid, fuming 37%, extra pure,
1 L, 2.5 L 100314
Ph Eur, BP, JP NF
Hydrochloric acid, fuming min. 37%,
1 L, 2.5 L 100317
Hydrochloric acid in ethanol 1 L, 5 L 100327 Ziehl-Neelsen staining
Hydroquinone, for synthesis 250 g, 1 kg 822333
Iodine 100 g, 500 g 104761 Weigert’s iodine solution
Iron(III) chloride for analysis EMSURE® 100 g, 250 g, 1 kg 103943 For preparing staining solutions
Kaiser’s glycerol gelatine 100 g 109242 Binder, aqueous mounting medium
Kaiser’s glycerol gelatine, phenol-free (NEW) 100 g 108635 Binder, aqueous mounting medium
Lactic acid, about 90%, extra pure,

500 mL, 1 L 100366
Ph Eur, BP, E 220
Lithium carbonate, extra pure, Ph Eur, BP, USP 250 g, 1 kg 105671 Addition to stain solutions
10
Lithium carbonate for analysis EMSURE® Cold-saturated aqueous solution, 1 g in 100 ml

100 g, 250 g 105680
ACS, Reag. Ph Eur distilled water
Methanol, extra pure, DAB, BP, NF 1 L, 2.5 L 106008
Methanol 1 L, 2.5 L 106009
Methylbenzoate, extra pure 1 L, 2.5 L 106059 Intermedium
Methyl salicylate, extra pure, Ph Eur, BP, NF 2.5 L 106070 Clearing agent
M-FREEZE cryo-embedding medium (NEW) 100 mL 103693 Embedding medium for frozen sectioning.
Molybdatophosphoric acid hydrate

25 g, 100 g 100532 Trichrome staining
Xylene substitute for histoprocessing,
Neo-Clear™ Xylene Substitute 5 L, 25 L 109843
deparaffination, dehydration
Nitric acid 65 %, extra pure 1 L, 2.5 L 100443
Nitric acid 65 % for analysis EMSURE ®

1 L, 2.5 L 100456 Visualization of neurofibrils, 3–7% solution
Perhydrol 30 % H2O2 for analysis EMSURE ACS,
® ®
250 mL, 1 L 107209
ISO, Reag. Ph Eur
Periodic acid 25 g, 100 g 100524 PAS reaction, 0.75 % solution
Phenol, extra pure, Ph Eur, USP 1 kg 100200
Phenol for analysis EMSURE ®

Preparing liquid phenol: melt 10 parts of phenol with
250 g, 1 kg 100206
ACS, ISO, Reag. Ph Eur slight heating, and add 1 part of distilled water
Phosphate buffer solution,
1L 107294 Buffer concentration of 0.025 M
pH 6.88
Platinum(II) chloride (73 % Pt), for synthesis 500 mg 824566 Aqueous solution 1:300 for fixation of mitochondria
Platinum(IV) chloride (57.5 % Pt) anhydrous,

1g 807347 Preparation of fixing mixtures
for synthesis
2-Propanol for analysis EMSURE ®
1 L, 2.5 L 109634 Fixing and dehydrating agent
2-Propanol, extra pure, Ph Eur, BP, USP 1 L, 25 L 100995
1-Propanol, extra pure 1 L, 2.5 L 100996
1-Propanol for analysis EMSURE ®

1 L, 2.5 L 100997
ACS, Reag. Ph Eur
Potassium aluminum sulfate for analysis
100 g, 1 kg 101047 Hemalum solutions
EMSURE® ACS, ISO, Reag. Ph Eur
Potassium carbonate, extra pure,
1 kg 104924
Ph Eur, USP, E 501
Potassium carbonate for analysis EMSURE ACS, ®
100 g, 500 g, 1 kg 104928 Preparing carmine solution
ISO, Reag. Ph Eur
Potassium chloride, extra pure,
1 kg 104935
Ph Eur, BP, USP, FCC, E 508
Potassium chloride, GR 100 g, 500 g, 1 kg 104936
Potassium chromium(III) sulfate for analysis

250 g, 1 kg 101036
EMSURE® ACS, ISO, Reag. Ph Eur (chrome alum)
Potassium cyanide for analysis EMSURE ®
100 g, 250 g, 1 kg 104967
ACS, Reag. Ph Eur
11
Potassium dihydrogen phosphate crystals,

1 kg 104871
extra pure, Ph Eur, BP, NF, E 340
Potassium dihydrogen phosphate for analysis
250 g, 1 kg 104873 Preparing buffer solution
EMSURE® ISO
Potassium dihydrogen phosphate solution,
1L 104875 Preparing buffer solution
1/15 M
Potassium hexacyanoferrate(II) trihydrate 100 g, 500 g 104984 Iron staining
Potassium hydrogen phthalate,

250 g, 1 kg 104874
for analysis EMSURE® Reag. Ph Eur
Potassium hydroxide, extra pure,
1 kg 105032
Ph Eur, BP, JP, NF, FCC, E 525
Potassium hydroxide pellets, GR 500 g, 1 kg 105033
Potassium iodide for analysis EMSURE ®

250 g, 500 g, 1 kg 105043 Weigert’s iodine solution
ISO, Reag. Ph Eur
Resorcinol, extra pure, BP, Ph Eur, USP 100 g, 1 kg 107590
Used for the preparation of staining solutions

Resorcinol 100 g, 250 g 107593
(Weigert’s Elastin)
To prepare 1/4-strength RINGER solution,
Ringer tablets 1 pack 100 tablets 115525
dissolve 1 tablet in 500 ml of distilled water
Salicylic acid, extra pure, Ph Eur, BP, USP 1 kg 100631
Schiff’s reagent 500 mL, 2.5 L 109033 PAS reaction
PAS method,
Schiff’s reagent Intense (NEW) 1L 102572
Detection of polysaccarides
Silver nitrate for analysis EMSURE®
25 g, 100 g, 250 g, 1 kg 101512 Silver staining
ISO, Reag. Ph Eur
Sodium carbonate, extra pure, anhydrous,
1 kg 106398
Ph Eur, BP, NF
Sodium carbonate, anhydrous for analysis
100 g, 500 g 106392
EMSURE® ISO
Sodium chloride, extra pure, Ph Eur, BP, USP 1 kg 106400
Sodium chloride cryst.

100 g, 500 g, 1 kg 106404 Preparing physiological saline solution
for analysis EMSURE® ISO
Sodium disulfite, for analysis EMSURE ACS,
®
100 g, 500 g, 1 kg 106528 Preparing sulfite solutions
Reag. Ph Eur
Sodium hydrogen carbonate, extra pure,
2.5 kg 106323
Ph Eur, BP, USP, FCC, E 500
Sodium hydrogen carbonate for analysis
100 g, 500 g, 1 kg 106329 Preparing buffer solution
EMSURE® ACS, Reag. Ph Eur
12
di-Sodium hydrogen phosphate, anhydrous,

100 g, 500 g, 2.5 kg 106586 Preparing buffer solution
di-Sodium hydrogen phosphate, extra pure,
5 kg 106585
anhydrous, Ph Eur, BP, USP, E 339
di-Sodium hydrogen phosphate 2-hydrate
500 g, 1 kg 106580 Preparing buffer solution
for analysis EMSURE®
di-Sodium hydrogen phosphate solution,
1L 106587 Buffer stock solution, preparing buffer solution
1/15 M
Sodium hydroxide solution,
2.5 L 105587
about 32%, extra pure
Sodium iodate for analysis EMSURE® 25 g, 100 g 106525 Preparing buffer solutions
di-Sodium succinate (hexahydrate)

500 g 820151
for synthesis
di-Sodium tetraborate, anhydrous, GR 100 g, 250 g 106306 Addition to silver solutions
Sodium tetraborate for analysis EMSURE® (Borax)

250 g 106310 Staining solutions
(85% Na2B4O7)
di-Sodium tetraborate decahydrate, extra pure,
1 kg 106303
Ph Eur, BP, NF
Sorbitol F liquid 50 L 102993 Substitute for glycerol in histological conservation
Pre-treatment of specimens in the detection

Sputofluol™ Pre-treatment Solution 1L 108000
of mycobacteria
5-Sulfosalicylic acid dihydrate for analysis As 5% solution or fixing mixture with ethanol and
100 g, 250 g, 1 kg 100691
EMSURE® ACS glacial acetic acid
Tetrahydrofuran, extra pure 1 L, 2.5 L 108114
Tetrahydrofuran for analysis EMSURE® ISO 1 L, 2.5 L 109731 Clearing agent
Additive in aqueous staining solutions, a few crystals

Thymol cryst. extra pure, Ph Eur, BP, NF 100 g, 1 kg 108167 of thymol’ in 100 ml of staining solution prevent
contamination
Toluene, extra pure 1 L, 2.5 L 108323 Intermedium
Toluene for analysis EMSURE ®

1 L, 2.5 L 108325 Intermedium
Trichloroacetic acidfor analysis EMSURE ®
100 g, 250 g, 1 kg 100807 5–10% solution
ACS, Reag. Ph Eur
Trichloroethylene, extra pure 1 L, 2.5 L 100958 Intermedium
3-(Triethoxysilyl)propylamine, for synthesis 100 mL 821619 Silanization: 2% in acetone for 5 min
Tris(hydroxymethyl)amino-methane
100 g, 500 g 108382 Buffer solution (Tris buffer)
Visualizing connective tissue,
Tungstophosphoric acid for analysis EMSURE® 100 g, 250 g 100583
fibrin and bones 1–5% (fixing agent)
Histological and cytological grade for developed
Xylene (isomeric mixture) (NEW) 4L 108298 specifically for processing, deparaffination,
dehydration
13
Dyes for Microscopy

Dyes are used in microscopy whenever cell and tissue
components in animal and plant material have to be visualized.
Frequently, such optical visualization techniques are the
only methods of identifying and differentiating such tissues.
Microscopy dyes are used mainly in histology, cytology and
microbiology but also in analyzing textile fibers, paper and other
technical products. They have even been used for identifying
the various layers of paintings. Dyes can be roughly divided
into acidic and basic dyes and synthetic and natural dyes. The
method introduced by Paul Ehrlich for using mixtures of acidic
and basic components was an important milestone in the
development of staining techniques. The basic dyes (methylene
blue, azure) stain the acidic components of the genetic material,
e.g. chromatin; and the acidic dyes (e.g. eosin) stain mainly the
basic proteins, e.g. the lysosomal enzymes in the granules.
The subsequent microscopic picture with its color intensity and

contrast is essentially determined by the quality of the solution
(pH, concentration, stability etc.) as well as the technical
procedure used.
14
2
Certistain® Dry Dyes for Microscopy
Certistain® dry dyes for microscopy are analyzed chemically according to strict specifications and tested for
their functional performance. The dyes guarantee consistent performance, batch to batch, and physical product
specifications including test protocols of each dye may be offered to interested customers.
Color Min. dye

Index-No. content in %
Fuchsin acid 25 g C.I. 42685 60 105231 Plasma: 1% aqueous solution
Vital and fluorescent stain: 0.5% in

Acridine orange ZnCI2 25 g C.I. 46005 60 115931
physiological sodium chloride solution
Alcian blue 8 GX 10 g C.I. 74240 – 105234 Acid mucosubstances: 1% in acetic acid (3%)
Brilliant cresyl blue ZnCI2 25 g – 60 101368 Reticulocytes: 1% in physiological saline solution
Brilliant green
25 g C.I. 42040 95 101374 Spores: 5% aqueous solution
(hydrogen sulfate)
Histological sections: 3.6% in aqueous
Carmine 5 g, 25 g C.l. 75470 1) 115933 saturated lithium carbonate solution
(boil for 15 minutes and add 1 g of thymol)
Cresyl violet (acetate) 25 g – 70 105235 Cell nuclei, tigroid plaques: 1% solution
Gram staining: dissolve 2 g in 20 ml ethanol (95%) and

Crystal violet 25 g, 100 g C.I. 42555 88 115940 mix with 80 ml of a 1% aqueous ammonium oxalate
solution
Eosin B (bluish) 25 g C.I. 45400 85 115934 Cell plasma: 0.1%–1% aqueous solution
Eosin Y (yellowish) 25 g, 100 g C.I. 45380 85 115935 Histological sections: 0.5% aqueous solution
Histological sections: 0.5% aqueous solution plant buds:

Erythrosin B 10 g, 25 g C.I. 45430 80 115936
0.5% solution in clove oils
Fast green FCF 25 g C.I. 42053 85 104022 Testicles: 0.2% solution in ethanol (95%)
Cell nuclei, bacteria: 10% solution in

Fuchsin 25 g, 100 g C.I. 42510 85 115937
prewarmed absolute ethanol
Cell nuclei: dissolve 0.5% in 10 ml ethanol dilute with 90
Hematoxylin monohydrate 25 g, 100 g C.I. 75290 95 115938 ml of distilled water and leave
to mature for 4–5 weeks
Light green SF (yellowish) 25 g, 100 g C.I. 42095 75 115941 Chromosomes: 0.2% solution in ethanol (95%)
Malachite green (oxalate) 25 g, 100 g C.I. 42000 90 115942 Spores: 5% aqueous solution
Methylene blue 25 g, 100 g C.l. 52015 82 115943 Bacteria: 1% aqueous solution
Chromatin: dissolve 1 g in 100 ml of distilled water and

Methyl green ZnCI2 25 g C.I. 42590 50 115944
add 25 ml absolute ethanol
Neutral red 25 g C.I. 50040 90 101376 Brain sections: 1.75% aqueous solution
New fuchsin 100 g C.I. 42520 75 105226 Staining of mycobacteria acc. to Ziehl-Neelsen
Bacteria: boil 10 g in 100 ml water for 10 min., leave to

Nigrosin 25 g C.I. 50420 1) 115924
cool and add 0.5 ml formaldehyde solution (37%)
1) Substance mixture without homogeneous structural formula
Skin, paraffin section, Masson-Goldner staining
15
Dyes for Microscopy
Color Min. dye

Index-No. content in %
Nile blue (hydrogen sulfate) 25 g C.I. 51180 90 115946 Fat liver: dissolve 0.05 g in 1% H2SO4 solution
Histological sections: dissolve 0.1 g together with 5 g

Nuclear fast red 25 g C.I. 60760 90 115939
aluminum sulfate in 100 ml hot water
Lipids: dissolve 0.5 g in 100 ml isopropyl
Oil red O 25 g C.I. 26125 80 105230
alcohol (60%), boil briefly and filter
Cell plasma: dissolve 1 g in 90 ml water and add 12.5 ml
Orange G 25 g C.I. 16230 80 115925
methanol
5 g, Histological sections: dissolve 0.4 g in 100 ml ethanol
Orcein – 1) 107100
25 g (70%) and add concentrated hydrochloric acid
25 g,
Pararosaniline (chloride) C.I. 42500 88 107509 1% clear, colorless solution as Schiff’s reagent
100 g
Histological sections: mix 5% aqueous solution with 1%
Phloxin B 25 g C.I. 45410 80 115926
aqueous borax methylene blue solution in a ratio of 1 + 9
Histological sections: mix 10 ml of a 1% aqueous solution
Ponceau S 25 g C.l. 27195 80 115927 with 90 ml of a saturated picric acid solution and add 1.5
ml of a 2% acetic acid solution
Histological sections: mix 12.5 ml of a 2% aqueous
solution (extracted by chloroform) with 7.5 ml of a 2%
Pyronine G 5g C.I. 45005 85 107518
aqueous methyl green solution (extracted by chloroform)
and add 30 ml distilled water
Safranine O 25 g C.I. 50240 80 115948 Chromosomes: 1% aqueous solution
Toluidine blue O 25 g C.I. 52040 85 115930 Histological sections: 0.1% aqueous solution
Oil red O color solution 102419
1) Substance mixture without homogeneous structural formula
Alcian blue-Nuclear fast red staining
16
Other Dry Dyes and Dye Mixtures
Color
Index-No.
Collagenic connective tissue:
0.05–0.1% amido black 10 B in saturated picric acid solution;
electrophoresis and redox indicator destaining solution for electrophoresis:
250 ml ethanol, 250 ml distilled water, 500 ml acetic acid,
Amido black 10 B 25 g C.I. 20470 101167
mix together;
staining solution for electrophoresis:
1.25 g amido black 10 B 250 ml destaining solution, mix the amido black
10 B and destaining solution together, filter before use
Microscopy
Auramine O
50 g C.I. 41000 101301 staining of mycobacteria acc. to Hagemann-Hermann,
fluorescence staining
Microscopy
blood smears, reticulocytes, protozoa: 1 g azure II in 100 g ethanol heated
to 50°C;
C.I. 52010 / mast cells: 4 ml 0.1% aqueous azure II solution 4 ml 0.1% aqueous eosin
Azure II 10 g 109211
52015 bluish solution 5 ml acetone 25 ml distilled water 2 ml buffer solution pH
4.1;
buffer solution: 2 ml citric acid (0.1 mol/l) 1 ml di-sodium hydrogen
phosphate (0.2 mol/l)
Brilliant green Microbiology
50 g – 101310
(hydrogen sulfate) culture medium additive for BPLS agar, BRILA broth etc.
Brilliant green
25 g C.I. 42040 101374 Spores: 5% aqueous solution
(hydrogen sulfate)
Calcon 50 g C.I. 15705 104594 Indicator dye
Microscopy carmalum acc. to Mayer: heat 10 g potassium aluminum sulfate

in 200 ml distilled water, add 1 g carminic acid, leave to cool and add 0.2 g
Carminic acid 5g C.I. 75470 100211 salicylic acid;
paracarmine acc. to Mayer: cautiously heat 1.0 g carminic acid, 0.5 g
aluminum chloride and 4.0 g calcium chloride in 100 ml 70% ethanol
Coomassie®* brilliant blue
25 g C.I. 42655 115444 Electrophoresis
G 250
Coomassie®* brilliant blue
25 g C.I. 42660 112553 Electrophoresis
R 250
4’,6-Diamidino-2-
Microscopy
phenylindole-dihydrochloride 100 mg – 124653
fluorescent staining: 1:1,000 to 1:10,000 with distilled water
(DAPI)
Eriochrome blue-black B 25 g C.I. 14640 103168 Indicator dye
Fluorescein Microscopy
250 mg – 124546
isothiocyanate (FITC I) visualise proteins
Dye mixture for microscopy blood smears, protozoa:
Giemsa’s 25 g, heat 0.76 g Giemsa’s azure-eosin methylene blue in 50 ml glycerol
– 109203
azure eosin methylene blue 100 g for 3 hours in a water bath at 60 °C, add 50 ml methanol;
leave to stand for 5 days and filter
25 g,
Microscopy
Hematoxylin cryst. 100 g, C.I. 75290 104302
nuclear staining
1 kg
Neutral red 25 g C.I. 50040 101376 Brain sections: 1.75% aqueous solution
17
Dyes for Microscopy
Other Dry Dyes and Dye Mixtures

Color
Index-No.
Microscopy
Leishman’s
10 g – 101350 blood smears: 0.12 g Leishman’s eosin-methylene blue dissolved in 100
eosin methylene blue
ml methanol heated to 40°C, leave to mature for 5 days and filter
Microbiology
Malachite green 25 g, 100 g, culture medium additive, spore staining acc. to Schaeffer-Fulton:
C.I. 42000 101398
(oxalate) 1 kg 5 g malachite green-oxalate in 100 ml distilled water;
vital nuclear stains of plant cells: 0.001–0.1 % aqueous solution
Dye for microscopy
May-Grünwald’s blood smears: 0.25 g May-Grünwald’s eosin methylene blue in
25 g, 100 g – 101352
eosin methylene blue 100 ml methanol with slight heating in a water bath at 60°C,
stir for 1 hour, leave to stand for 24 hours and filter
Microscopy
collagenic and reticular connective tissue;
Methyl blue 50 g C.I. 42780 116316
Microbiology
culture medium e.g. Gassner agar
25 g, 100 g,
Methyl orange C.I. 13025 101322 Indicator dye
1 kg
Methyl red 25 g, 100 g C.I. 13020 106076 Indicator dye
Microscopy
Nitro blue tetrazolium (NBT) 500 mg – 124823
detection of succino-dehydrogenase, NBT test
Microscopy
Rhodamine B 25 g, 100 g C.I. 45170 107599
vital and fluorescent staining: solution 1:1,000 in tap water
Tetrachloroauric(III) acid 1 g, 5 g
– 101582 Demonstration of glia structures by gold impragnation: 1% solution
trihydrate 99% (ampoules)
Vital staining: 1:10,000 with physiological saline solution or
Thymol blue, ACS 5 g, 25 g – 108176
Ringer solution; indicator dye
Titan yellow 25 g C.I. 19540 101307 Reagent for magnesium
2,3,5-Triphenyltetrazolium- Microbiology
10 g, 100 g – 108380
chloride culture medium additive for TTC broth
Microscopy
Trypan blue 25 g C.I. 23850 111732
vital staining: 0.5 g in 100 ml distilled water
Dye for microscopy
Wright’s eosin methylene blue 25 g – 109278 blood smears: 0.24 g Wright’s eosin methylene blue in 100 ml methanol;
leave to stand for 5 days and filter
Sputum, smear, Pleura effusion, smear,

mycobacteria, cold staining Wright staining
18
Fluorescence Dyes
In fluorescence microscopy, preparations are irradiated with short-wave light and the long-wave light (fluorescence)
emitted as a result by certain structures or dyes is observed. Long-wave ultraviolet (UV) light, or blue-violet light,
is used for this purpose. Initially, the natural fluorescence emitted by samples was analyzed; later, non-fluorescing
preparations were labeled with small quantities of fluorescing dye and analyzed under the microscope. Fluorescent
dyes are used for the staining and visualization of cells, cell components, chromosomes, bacteria and for the detection
of antigen-antibody reactions.
Emission Fluorescence
(nm) (nm)
Acid fuchsin
25 g 540 630 105231 Bone
Certistain® Dry Dyes
Acridine orange
25 g 470 530–650 115931 Bacteria, DNA
Auramine O 50 g 460 550 101301 Mycobacteria
Calcein indicator 5g 102315 Bone
4’,6-Diamidino-2-phenylindole
100 mg 340 488 124653 Chromosomes, chlamydia
dihydrochloride (DAPI)
Fluorescein-5-isothiocyanate
250 mg 490 525 124546 Proteins antigen-antibody reactions
(FITC)
Pararosaniline
25 g 570 625 107509 DNA Feulgen
Pyronine G
25 g 410 540 107518
Rhodamine B 25 g, 100 g 540 625 107599 Proteins
Lung, paraffin section,

Tb-fluor phenol-free staining,
top: rhodamine staining

bottom: auramine staining
19
Products for
Bacteriology
Bacteriology is an independent discipline within the scope of
clinical diagnosis and industrial quality control. In order to detect
microorganisms in microscopic analysis they have to be stained with
suitable dyes. Gram-staining and the detection of mycobacteria are of
particular importance. Bacterial staining, with the exception of supra-vital
staining (e.g. fluorescent staining), is carried out on heat-fixed cells.
20
3
Staining Kits for Bacteriology
Gram-color
Gram-staining enables bacteria to be differentiated into Gram-positive and Gram-negative types. In this process,
aniline dye reacts with iodine in the cell wall to form an iodine-dye complex. In the case of Gram-positive bacteria, the
iodine-dye complex cannot be released from the cell using destaining agents such as alcohol or acetone; the cell thus
retains its blue-violet color. In the case of Gram-negative bacteria, the iodine-dye complex is dissolved and the cell
can be counter-stained with safranin orange or carbol fuchsin to produce a pink to red color.
1 pack
Staining kit for Gram staining on staining bench,
500 mL crystal violet
in jars and staining instruments
solution,
500 mL Lugol’s solution
Gram-color 111885
stabilized with PVP,
Result:
2 x 500 mL decolorisation
Gram-positive bacteria: dark violet
solution,
Gram-negative bacteria: orange
500 mL safranine solution
Gram individual solutions
Gram’s crystal violet solution 500 mL, 2.5 L 109218
Gram’s safranine solution 500 mL, 2.5 L 109217
Gram’s decolorizing solution 500 mL, 2.5 L 110218
1% aqueous solution of iodine (0.33%) and

Lugol’s solution 250 mL, 1 L 109261
potassium iodide (0.67%)
Lugol’s solution stabilized with PVP 1 L, 2.5 L 100567 For the Gram stain
Gram-color Modified, Phenol-free
1 pack
100 mL crystal violet solution,
Staining kit for modified Gram staining on staining bench
100 mL sodium hydrogen
carbonate solution.
Gram-color modified, phenol-free Bottle for working solution, 101603
Result:
190 mL iodine solution,
Gram-positive bacteria: dark violet
PVP stabilized,
Gram-negative bacteria: red
190 mL decolorisation solution,
190 mL fuchsin solution
Culture, smear,
Gram-color modified,
phenol-free staining
21
Products for Bacteriology
Staining of Mycobacteria
Tb-color
Tb-color is a staining kit for the microscopic analysis of mycobacteria. The Tb-color staining technique is based on a
modified Ziehl-Neelsen method which alleviates the need for heating the carbol fuchsin solution. Thus, the release
of toxic phenol vapor is circumvented. The bright red acid-resistant rods can be clearly seen against the green
background.
Staining kit for cold staining method in jars and

1 pack
staining instruments
500 mL Sputofluol™
Pre-treatment Solution,
Tb-color Staining Kit 116450
500 mL carbol fuchsin solution,
Result:
500 mL hydrochloric acid alcohol,
acid-fast bacteria: red
500 mL malachite green solution
background: green
Tb-color individual solutions
Tb-color Carbol fuchsin solution 500 mL, 2.5 L 108512 Cold staining of mycobacteria
Tb-color malachite green

500 mL, 2.5 L 110630 Counter-staining
(oxalate) solution
Pre-treatment of specimens in the detection
Sputofluol™ Pre-treatment Solution 1L 108000
of mycobacteria
Hydrochloric acid in ethanol 1 L, 5 L 100327 Decolorisation solution (0.75% hydrochloric acid)
Tb-color Modified
Tb-color modified 1 pack 100497 Staining kit for the detection of mycobacteria
500 mL carbol fuchsin solution, (AFB) by the hot staining method
2 x 500 mL decolorisation solution,
500 mL methylene blue solution
Result:
background: light blue
Tb-Fluor
The Tb-Fluor staining kit is used for the fluorescence detection of mycobacteria under the microscope. The
mycobacteria are stained with auramine-rhodamine solution; depending on the filter combination used, the
mycobacteria appear either red or green and can be clearly seen against the dark background. Tb-Fluor can be used
for sputum, pleural effusions, bronchial lavage, urine and for histological sections.
Fluorescence microscopical detection of mycobacteria

1 pack
500 mL auramine-rhodamine solution,
Tb-fluor 3 x 500 mL decolorisation solution, 109093
Result:
2 x 500 mL counter-staining solution
acid-fast bacteria: red or yellow-green fluorescence
(KMnO4)
background: dark
Fluorescence staining kit for the microscopic
1 pack
investigation of mycobacteria
200 mL auramine-rhodamine staining
solution, phenol-free,
TB-fluor phenol-free 101597
2 x 200 mL decolorisation solution,
Result:
200 mL counter-staining solution
acid-fast bacteria: red or yellow-green fluorescence
(KMnO4)
background: dark
22
Trichomonad Staining
For the visualization of trichomonads in vaginal smears and urine sediment using Neo-Cytocolor™ rapid staining
kit. For a more environmentally-friendly option, the Neo-Cytocolor™ rapid staining kit includes Neo-Clear™ xylene
substitute which is comprised of non-aromatic solvents instead of xylene. This reduces the inhalation risks, reactivity,
and toxicity from exposure to xylene.
1 pack
Cytocolor™ 500 mL modified hematoxylin solution Modified Papanicolaou staining
Rapid Staining 500 mL modified polychrome solution 115355 Trichomonads:
Kit 3 x 500 mL 2-propanol GR gray-blue to gray-green
500 mL Xylene
1 pack
Neo-Cytocolor™ 500 mL modified hematoxylin solution Fast staining kit for a modified Cervix smear,
Rapid Staining 500 mL modified polychrome solution 101971 Papanicolaou stain, with Neo-Clear™ Cytocolor™ rapid staining kit
Kit 3 x 500 mL 2-propanol GR Xylene Substitute for clearing
500 mL Neo-Clear™ Xylene Substitute
Ziehl-Neelsen-staining
Hot staining for acid-fast bacteria,

counter-staining with methylene blue
Ziehl-Neelsen carbol-fuchsin
100 mL, 500 mL, 2.5 L 109215
solution
Result:
background: light blue
Staining Solutions for Bacteriology
Giemsa’s azure eosin Staining of spirochetes:

methylene 100 mL, 500 mL, 1 L, 2.5 L 109204 Spirochetes pallida light-pink,
blue solution other spirochetes reddish to bluish-violet
Staining of fungi:
Lactophenol blue add 1 or 2 drops of lactophenol blue
100 mL 113741 Culture, smear,
solution solution to the specimen; visualize under
Lactophenol blue staining
the microscope after approx. 2 min
Methylene blue staining is suitable for
general bacteriological control stainings,
Löffler’s methylene
100 mL, 500 mL, 2.5 L 101287 e.g. of gonococcae, lactic acid bacteria
blue solution
and for visualization of the pole corpuscles
of pasteurella
23
Products for Bacteriology
Other Products and Auxiliaries for Bacteriology
Anaerobic jar 1 unit 116387 Volume 2.5 l
Stain for flagellum staining,

chromium acid solution: chromium(Vl)-oxide 2.5 g; aqua
Chromium(VI)-oxide 250 g 100229
dest. ad 100 ml.
Tanning mixture see tannic acid
Gelatine 500 g 104070 Bloom number about 200
1 pack
Gentamycin solution 111977 Microbiology and virology additive for culture media
10 mL
Reagent solution for detection of nitrite produced by
GRIESS-ILOSVAY’S nitrite reagent
500 mL 109023 microorganisms, identification of nitrate-reducing
microorganisms
Immersion oil 100 mL, 500 mL 104699 Viscosity about 100–120 mPa·s
Manufactured according to the international standard ISO

Immersion oil, acc. to ISO 8036, Halogene-Free (NEW) 100 mL 103699
8036. Lower toxicity.
KOVACS‘ Indole reagent 100 mL 109293 Detection of Indole formation
Lactic acid about 90% 2.5 L 100366 Neisser’s staining: 0.9 ml to 100 ml Lugol’s solution
Plate basket (for anaerobic jar) 1 pack 107040 12 petri dishes
1 pack
RINGER tablets 115525 Preparation of an 1/4 strong Ringer’s solution
100 tablets
Sodium hydroxide solution Visualization of fungi in a native preparation:
1L 105588
min. 10% (1.11) warm carefully 1 drop on the slide
Sodium thioglycolate 500 g 106691 Culture medium additive for anaerobic bacteria
1 pack
Sterikon® plus bioindicator 15 ampoules, 110274 Controlling autoclaving
100 ampoules
Tanning for flagellum: tannic acid solution: 20 g in 100 ml
aqua dest. tanning mixture: mix 100 ml tannic acid
Tannic acid powder 1 kg 100773
solution with 15 ml chromium acid solution; store 2 days
at room temperature, filter before use
Detection of fluorescence after MUG decomposition by E.
UV lamp 366 nm 1 pack 113203
coli for microbiology
Culture,
Löffler's methylene blue staining
24
Products for Cytology
Cytology is a technique used to differentiate tumors from other
inflammatory or degenerative diseases. The advantages of the method
are the ease of obtaining material and the relative ease of processing.
Cytology is highly specific and accurate. These advantages make it
suitable for screening purposes and have in fact led to a significant
reduction in the incidence of cervical cancer. The acceptance of
gynecological cytology as a valuable discipline in cancer screening is
principally due to the work of Dr. George N. Papanicolaou (1883 – 1962),
the “Father of modern cytology“.
4 25
Staining Solutions for Cytology

Papanicolaou Solutions
The cytological staining technique developed by Papanicolaou is still the standard method in cancer and hormone cycle
diagnosis. Using the standard Harris hematoxylin solution (Papanicolaou solution 1a), excellent nucleic staining can
be achieved in 3 – 5 minutes; the modified hematoxylin solution S (Papanicolaou solution 1b) provides good nucleic
staining in 2 – 3 minutes. Differential cytoplasmic staining can be achieved using orange stains in conjunction with
polychrome solutions. Four different polychrome solutions are available; they differ in the various concentrations
of the components – light green SF, eosin Y and Bismarck brown – and produce different cytoplasmic stains. In
cytological gynecology, polychrome solutions EA 31 (3a) and EA 50 (3b) are mostly used. In non-gynecological
cytology, polychrome solutions EA 65 (3c and 3d) can be used for the analysis of sputum, bronchial, gastric and
intestinal secretions.
Papanicolaou’s solution 1a
500 mL, 1 L, 2.5 L 109253 Nuclear staining
Harris’ hematoxylin solution
Papanicolaou’s solution 1b
500 mL, 2.5 L 109254 Nuclear staining
Hematoxylin solution S
500 mL, 1 L, 2.5 L 106888 Cytoplasm staining of mature and keratinized cells
Orange G solution (OG 6)
500 mL, 2.5 L 106887 Cytoplasm staining of mature and keratinized cells
Orange II solution
500 mL, 2.5 L 109271 Cytoplasm staining
Polychrome solution EA 31
500 mL, 1 L, 2.5 L 109272 Cytoplasm staining
Polychrome solution EA 50
Papanicolaou’s solution 3c Cytoplasm staining esp. for non-gynecological specimen,
100 mL 109270
Polychrome solution EA 65 color effect light red
Papanicolaou’s solution 3d Cytoplasm staining esp. for non-gynecological specimens,
100 mL, 2.5 L 109269
Polychrome solution EA 65 color effect pale blue-green
Cytocolor™ and Neo-Cytocolor™ Rapid Staining Kits

Cytocolor™ rapid staining kit is capable of producing
stains within 3 minutes. These stains provide complete
information on integrity and additional infomation on
hormone status and vaginal flora in gynecological smears.
The difference between these and classical Papanicolaou
stains is that Cytocolor™ rapid staining kit requires no
orange stain; both mature and keratinized cells appear
pink instead of orange. Neo-Cytocolor™ rapid staining kit Pleural effusion, smear, Gynecological smear,
also produces a stained slide with complete information Cytocolor™ rapid staining kit Papanicolaous staining
on integrity, hormonal status and bacterial condition. The
primary benefit of the Neo-Cytocolor™ rapid staining kit is
that it is a greener, more user-friendly option due to the
inclusion of the Neo-Clear™ xylene substitute. The kit has a
capacity for up to 1,000 slides and a shelf life of 3 years.
1 pack
Cytocolor™ Rapid Staining Kit 500 mL mod. hematoxylin solution
cytological standard stain 500 mL mod. polychrome solution, 115355 Rapid staining kit for modified Papanicolaou’s staining
acc. to Szczepanik 3 x 500 mL 2-propanol GR,
500 mL xylene
1 pack
500 mL modified hematoxylin solution
Neo-Cytocolor™ Rapid Staining Fast staining kit for a modified Papanicolaou stain,
500 mL modified polychrome solution 101971
Kit with Neo-Clear™ Xylene Substitute for clearing
3 x 500 mL 2-propanol GR
26
Hematoxylin Solutions Modified According to Gill
These staining solutions are prepared using Certistain® hematoxylin dry dyes. Their balanced formulation means that
there is no need for filtration before use.
Hematoxylin solution modified acc. Gill ll 500 mL, 2.5 L 105175 Nuclear staining, progressive
Hematoxylin solution modified acc. Gill lll 500 mL, 1 L, 2.5 L 105174 Nuclear staining, progressive and regressive
Shorr Solution
In contrast to Papanicolaou staining, Shorr staining is
used exclusively in cytological hormonal applications.
However, in such applications, they are superior to
Papanicolaou staining as they can differentiate better
between eosinophilia and cyanophilia.
The ratio of eosinophilic to cyanophilic cells enables

information to be gathered relating to the effect of follicle Portio smear,
and corpus luteum hormone. The number of eosinophilic Shorr staining
cells increases under the effect of follicle hormone

whereas cyanophilic cells increase under the corpus
luteum hormonal effect.
Shorr Staining solution 500 mL 109275 Diagnosis of hormonal disorders
Other Staining Solutions
Giemsa’s azure eosin methylene

100 mL, 500 mL, 1 L, 2.5 L 109204 Staining of non-gynecological specimen
blue solution
Hemacolor Rapid Staining Kit
®
1 pack
111661 Fast staining of non-gynecological specimen
of Blood Smear 3 x 500 mL, 6 buffer tablets
®
1 pack
111674 Fast staining of non-gynecological specimen
of Blood Smear 3 x 100 mL, 3 buffer tablets
®
2.5 L 111955 Fixing solution (contains methanol)
Solution 1
®
2.5 L 111956 Color reagent red (eosin solution)
Solution 2
®
2.5 L 111957 Color reagent blue (thiazine solution)
Solution 3
1 pack 200 mL staining
Staining set containing staining solution, buffer solution and
Auto-Hemacolor™ Rapid Staining Kit solution, 480 buffer solution, 115213
washing solution
950 mL rinse solution
Lymph node, paraffin section,

Giemsa's staining
27
Other Products and Auxiliaries for Cytology

Catalog
Product Size Directions for use
No.
M-FIX® Spray Fixative 100 mL, 1 L (refill) 103981 Spray fixative for cytodiagnosis
Entellan™ Mounting Medium 500 mL 107960 Permanent preparations
Permanent preparations, without bubble formation at

high ambient temperatures
Permanent preparations especially for the
Entellan™ new for cover slipper 500 mL 100869
application with cover instruments
DPX new 500 mL 100579 Permanent preparations
Mounting agent for homogenous coating of

M-GLAS® Glass Coverslip 500 mL 103973
cytological smears, instead of cover slips
100 mL (dropper bottle), Mounting medium in conjunction with Neo-Clear™
Neo-Mount™ Mounting Medium 109016
500 mL Xylene Substitute
Neo-Clear™ Xylene Substitute 5 L, 25 L 109843 Substitute for xylene
Ethanol, absolute, for Analysis EMSURE® 1 L, 2.5 L 100983 Dehydration
Ethanol, denatured with about 1% methyl Ethyl Ketone

1 L, 5 L 100974 Dehydration
for Analysis EMSURE®
2-Propanol 1 L, 2.5 L 109634 Substitute for ethanol
Manufactured according to the international standard

ISO 8036. Lower toxicity.
Xylene (isomeric mixture) (NEW) 4L 108298
specifically for processing, deparaffination, dehydration
Clinical samples,
Hemacolor® rapid staining kit
28
Products for Hematology
Panoptic staining according to Pappenheim, and staining according
to Giemsa, Wright or Leishman have long been standard techniques
in hematological diagnostic procedures. Formerly, practically all
hematological samples were analyzed using manual staining methods.
Currently, most samples are analyzed using semi- or fully automatic
hematological systems capable of determining all the parameters
necessary for diagnosis. Pathological or suspect blood and bone marrow
smears are then subjected to classical differential analysis using stains.
Cytochemical methods are used for the differential diagnosis

of leukaemic disease and if necessary supplemented by
immunohematological methods.
5 29
Staining Kits and Solutions for Hematology

Hemacolor® Rapid Staining Kit
Hemacolor® rapid staining kit is comprised of 3
ready-to-use solutions – a fixing solution, a red and
a blue solution. Buffer tablets are supplied with the
kit; they can be used to prepare a phosphate buffer
solution of pH 7.2 according to Weise which guarantees
reproducible staining results.
Hemacolor® rapid staining kit produces a stain Blood smear,

corresponding to Pappenheim. The procedure requires Hemacolor® rapid staining kit
ca. 1/2 min and can be carried out manually or
automatically. Apart from time-saving, the shelf life of
the solutions is a further advantage. In addition to blood
and bone marrow smears, Hemacolor® rapid staining kit
can be used in clinical cytology, e.g. for staining urine,
sputum, FNAB, sperm, effusions and lavages.
Hemacolor Rapid Staining Kit of 1 pack

®
111674 Staining set for hematological and clinical specimen
Blood Smear (3 x 100 mL, 3 buffer tablets)
Hemacolor Rapid Staining Kit of 1 pack
®
111661 Staining set for hematological and clinical specimen
Blood Smear (3 x 500 mL, 6 buffer tablets)
Single solutions

2.5 L 111955 Fixing solution (contains methanol)
Solution 1
2.5 L 111956 Color reagent red (eosin solution)
Solution 2
2.5 L 111957 Color reagent blue (thiazine solution)
Solution 3
Other Staining Solutions
Giemsa’s azure eosin methylene 100 mL, 500 mL, 1 L, Differential blood staining; demonstration of blood parasites
109204
blue solution 2.5 L and protozoa (dilute approx. 1:20)
Leishman’s eosin methylene
500 mL 105387 Differential blood staining
blue solution
May-Grünwald’s eosin methylene 100 mL, 500 mL, 1 L,
101424 Differential blood staining
blue solution 2.5 L
Wright’s eosin methylene
100 mL, 500 mL, 2.5 L 101383 Differential blood staining
blue solution
30
Dye Mixtures for Hematological Staining Solutions
Dye mixture, for self-preparation of staining

Giemsa’s azure eosin methylene blue 25 g, 100 g 109203
solution, insert sheet in internet or on demand
Leishman’s eosin methylene blue 10 g 101350
May-Grünwald’s eosin methylene blue 25 g, 100 g 101352
Wright’s eosin methylene blue 25 g 109278
Auto-Hemacolor™ Rapid Staining Kit

The Auto-Hemacolor™ Rapid Staining Kit was developed for use in conjunction with the HEMA-TEK* Slide Stainer
for automatic staining of blood and bone marrow smears. The kit comprises staining solution, buffer and washing
solution.
1 pack
200 mL staining solution, Staining set containing staining solution, buffer solution and
Auto-Hemacolor™ Rapid Staining Kit 115213
480 buffer solution, washing solution
950 mL rinse solution
Blood smear,
Pappenheim staining
31
Cytochemical Reagents
Cytochemical stains are used for localizing and determining the activity of cellular components and enzymes. In
hematology, PAS, peroxidase, esterase and phosphatase reactions play an important role in leukaemia typing.
LEUCOGNOST™ Leukemia Detection Kit

LEUCOGNOST™ leukemia detection kit provide the user with all the necessary reagents and easy-to-follow staining
instructions. LEUCOGNOST™ leukemia detection kit now enable differential analysis for leukaemia to be carried out in
international FAB classes by smaller laboratories, hence making the technique no longer restricted to larger diagnostic
centers.
LEUCOGNOST ALPA
™
1 pack
116300 Detection of the alkaline leukocyte phosphatase activity in leukocytes
Leukemia Detection Kit 12 staining batches
LEUCOGNOST EST
™
1 pack
116301 Detection of the alpha naphthyl acetate esterase reaction in leukocytes
LEUCOGNOST PAS
™
1 pack
116302 Detection of the Periodic Acid Schiff reaction in leukocytes
LEUCOGNOST POX
™
1 pack
116303 Detection of the peroxidase reaction in leukocytes
LEUCOGNOST AP™
1 pack
116304 Detection of the acid phosphatase reaction in leukocytes
Detection of the naphthol AS-D chloroacetate esterase reaction
LEUCOGNOST™ NASDCL new 1 pack in leukocytes
117198
Leukemia Detection Kit (NEW) 12 staining batches
New formulation, more delicate staining
LEUCOGNOST™ Fixing Mixture 500 mL 112327 Fixing reagent for diagnosis with LEUCOGNOST™ leukemia detection kit
Blood smear,
LEUCOGNOST™ NASDCL new
Leukemia Detection Kit
Paraffin section, illiac crest punch

LEUCOGNOST™ NASDCL new
Leukemia Detection Kit
32
Bone marrow, paraffin section,
HEMATOGNOST Fe™ staining kit
HEMATOGNOST Fe™ Staining Kit

HEMATOGNOST Fe™ staining kit contains all the necessary reagents for carrying out the visualization of erythrocyte iron
in a simplified manner. The Berlin Blue staining technique used provides valuable diagnostic information on numerous
diseases connected with the utilization of iron (e.g.anemia, thalassemia) and can also be used with tissue sections (e.g.
liver sections, in cases of suspect hemochromatosis).
1 pack Iron stain (Prussian blue) in bone marrow sections and blood
HEMATOGNOST Fe™ Staining Kit 112084
8 staining batches smears and also in tissue sections
Liver, paraffin section,

hemachromatosis,
HEMATOGNOST Fe™ staining kit
Reagents for Manual Blood Cell Counting

The counting of erythrocytes, leukocytes, thrombocytes and reticulocytes is an important part of routine hematological
diagnosis. Today, this is mostly done on automatic instruments. Manual counts are carried out if the density is very
low or in particularly difficult cases.
Staining of reticulocytes; the Substancia Granulo-Villamentosa

Brilliant cresyl blue solution 100 mL 101384
of the reticulocytes become black-blue
Türk’s solution 100 mL, 500 mL 109277 Counting leukocytes in the counting chamber
33
Other Products and Auxiliaries for Hematology
Reagent solution B with LEUCOGNOST™ EST leukemia

Acetone 1 L, 2.5 L 100014
detection kit
Aquatex® Mounting Medium 50 mL 108562 Aqueous mounting medium in cytochemistry
Rapid mounting agent, permanent slides, no bubble

formation at high ambient temperatures (contains xylene)
absolute for analysis EMSURE® ACS, ISO, Reag. Ph Eur
Ethanol 1L 100983 Diluent for LEUCOGNOST™ POX leukemia detection kit,
reagent 1
denatured with about 1 % methyl ethyl ketone for analysis
Ethanol 1 L, 2.5 L 100974 EMSURE®
Dehydration agent
Hematoxylin solution modified
acc. to Gill III
Immersion oil, acc. to ISO 8036, Manufactured according to the international standard ISO 8036.
100 mL 103699
Halogene-Free (NEW) Lower toxicity.
Mayer’s hemalum solution 500 mL, 1 L, 2.5 L 109249 Counter-staining in cytochemistry
Methanol 1 L, 2.5 L 106009 Fixing and dehydration agent
Buffer tablets pH 6.4 1 pack 100 tablets 111373 Buffer solution/rinsing solution pH 6.4
Reagent solution for LEUCOGNOST™ PAS leukemia

Schiff’s reagent 500 mL, 2.5 L 109033
detection kit
PAS method,
Detection of polysaccarides
Titriplex® III 100 g, 200 g 108418 Preparation of EDTA blood
Buffer tablets pH 7.2

stabilized standard stainings
of blood and bone marrow
34
Products for Histology
In his book “Cellular Pathology“, in 1856, Rudolf Virchow stated quite
clearly that the diagnosis and prognosis of numerous diseases can
be facilitated by investigating cells and tissues under the microscope.
This was the birth of histopathology in diagnostic medicine. Numerous
staining techniques were initially developed in an empirical way for
analyzing tissue sections; the staining and recognition of cell nuclei,
cytoplasm, intra- and extra-cellular components was then made possible
by the development of more and more specific staining mixtures.
Classical techniques are still adequate in 90–95% of diagnoses. In
5–10% of cases, where diagnosis cannot be determined as being certain,
additional differential staining and visualization methods must be used.
These enable additional morphological criteria and functional properties
to be evaluated, hence making diagnosis more reliable. Such techniques
include histochemical stains, immunohistochemical methods, DNA
hybridization, FISH, PCR, flow cytometry etc.
6 35
Staining Solutions for Histology
Mayer’s hemalum solution 500 mL, 1 L, 2.5 L 109249 Nuclear staining
Hematoxylin solution
modified acc. to Gill III
Hematoxylin solution
500 mL, 2.5 L 105175 Nuclear staining
modified acc. to Gill II
Eosin Y solution 0.5% aqueous 1 L, 2.5 L 109844 Counterstaining in H&E
Eosin Y solution 0.5% alcoholic 500 mL, 2.5 L 102439 Counterstaining in H&E
Eosin Y solution 1% alcoholic 1L 117081 Counterstaining in H&E
Eosin-Phloxine solution, alcoholic 1L 102480 Counterstaining in H&E
Alcian blue solution 500 mL 101647 Alcian blue PAS staining
Giemsa’s 100 mL, 500 mL,

109204 Staining of bone marrow sections and lymph node sections
azure eosin methylene blue solution 1 L, 2.5 L
Picrofuchsin solution acc. to van
500 mL 100199 for connective tissue staining
Gieson
ELASTIN color solution acc. to Weigert 500 mL 100591 Visualisation of elastic fibers
Visualisation of neutral lipids

Oil red O color solution 250 mL 102419
in cryosection
Nuclear fast red-aluminum sulfate for nuclear staining and for counterstaining in histochemical
500 mL 100121
solution 0.1% reactions e.g. Berlin blue
PAS method,
Schiff’s reagent 500 mL, 2.5 L 109033
Detection of polysaccharides
PAS method,
Detection of polysaccharides
Periodic acid solution 0.5% aqueous 1L 100482 PAS method
Osmic acid solution 2% 5 ml (ampoule) 109266 Yellowish-brown coloration has no effect on usability
1–2% aqueous solution, several hours necessary to dissolve the

100 mg, 500 mg, 1 g
Osmium(VIII) oxide 124505 crystals, use only redistilled water for preparing the solution, a
(ampoules)
black-colored solution is unusable
Paraffin section kidney, Paraffin section tongue

Schiff‘s reagent Intense H&E staining, Eosin-Phloxine
solution
Liver, paraffin section, Congo

red staining
top: light microscopy
bottom: polarised light
36
Staining Kits for Histology
1 pack
Weigert’s iron hematoxylin kit 500 mL Weigerts’s solution A, 115973 Nuclear staining in connective tissue stainings
500 mL Weigert’s solution B
1 pack
500 mL Weigert’s solution A,
500 mL Weigert’s solution B,
Elastica van Gieson staining kit 115974 Demonstration of elastic fibers
500 mL Elastin solution acc. to Weigert,
500 mL Picrofuchsin solution
acc. to. van Gieson
1 pack
500 mL azophloxin solution,
500 mL tungstophosphoric acid
Masson-Goldner staining kit 100485 Visualization of connectiv tissue
orange G solution,
500 mL light green SF solution,
500 mL acetic acid solution 10%
1 pack
PAS staining kit 500 mL Schiff’s reagent, 101646 Visualization of muco-polysaccharides
500 mL periodic acid solution
1 pack
Congo red staining kit 100 mL congo red solution, 101641 For detection of amyloid acc. to Highman
2 x 100 mL KOH solution
1 pack
100 mL periodic acid solution,
3 x 100 mL silver nitrate solution,
Methenamine silver plating kit Visualization of basal membranes,
10 x methenamine borat tablets, 100820
acc. to Gomori bacteria and fungi
100 mL gold chloride solution,
100 mL sodium thiosulfate solution,
100 mL light green SF solution
1 pack
500 mL Silver nitrate solution 6%,
Warthin-Starry sílver plating kit
2 x 14 g Hydroquinone mixture, 102414 Detection of Helicobacter pylori in paraffin sections
modified
130 g Gelatine powder,
60 mL Acetic acid solution 1.2%
1 pack
16 mL potassium-permanganate solution,
16 mL sulfuric acid solution,
30 mL oxalic acid solution,
Reticulin silver plating kit acc. to
30 mL ammoniumiron(III)-sulfate solution, 100251 Detection of reticular fibres in paraffin sections
Gordon & Sweets
30 mL ammoniakalic silver nitrate solution,
30 mL formaldehyde solution,
30 mL gold chlorid solution,
30 mL sodium thiosulfate solution
1 pack
Silver plating kit acc. to von Kossa 100 mL silver nitrate solution 100362 Detection of calcium deposits in paraffin sections
100 mL sodium thiosulfate solution
1 pack Detection of free ionic iron with Prussian
HEMATOGNOST Fe™ Staining Kit 112084
8 staining batches blue method in tissue sections
1 pack
500 mL carbol fuchsin solution, Detection of mycobacteria in histological
Tb-color modified 100497
2 x 500 mL decolorisation solution, sections
500 mL methylene blue solution
1 pack
500 mL auramine-rhodamine solution,
Tb-fluor 109093 Fluorescence method for mycobacteria detection
500 mL decolorisation solution,
500 mL counterstaining solution
1 pack Fluorescence staining kit for the microscopic
200 mL auramine-rhodamine staining investigation of mycobacteria
TB-fluor phenol-free solution, phenol-free, 101597 Result:
2 x 200 mL decolorisation solution, acid-fast bacteria: red or yellow-green fluorescence
200 mL counter-staining solution (KMnO4) background: dark
1 pack
2 x 250 mL 5 M HCl, Feulgen staining for quantitative DNA
DNA staining kit according to Feulgen 107907
2 x 250 mL Schiff’s reagent, measurement
250 mL sodium disulfite solution conc.
37
ISOSLIDE™ Control Slides

To ensure that the test preparations contain the material relevant for diagnostics and the target structures can be
visualized by staining with good differentiation, it is recommended to use a control preparation. ISOSLIDE™ control
preparations are a group of products created to help optimize and standardize the staining result by enabling a direct
comparison of typical control material with laboratory-internal specimens. The ISOSLIDE™ control slides consist of
microscope slides with a paraffin section containing the typical structures of the test method to be used.
The control slide is prepared using sections of suitable animal material. Each pack of ISOSLIDE™ control slides contains
25 slides. One slide is stained with the reference method and is used for comparison. The 24 unstained slides are
stained according to the corresponding Merck specification or else can be stained according to the laboratory-internal
specification.
The unstained control slides are stained and subsequently compared with the laboratory material and the stained
control section regarding the staining result.
Control Slides with reference tissue for the detection of reticular fibers in
ISOSLIDE™ Reticulin
25 control slides 100361 histological tissue, 24 unstained paraffin sections, 1 stained control slide with
Control Slides
Reticulin silver plating acc. to Gordon & Sweets, Cat. No. 100251
Control Slides with reference tissue for the detection of free ionic iron in
ISOSLIDE™ Iron
25 control slides 100380 histological tissue, 24 unstained paraffin sections, 1 stained control slide
Control Slides
with HEMATOGNOST™ Fe staining kit, Cat. No. 112084
Control Slides with reference tissue for the PAS staining in histological
ISOSLIDE™ PAS
25 control slides 100408 tissue, 24 unstained paraffin sections, 1 stained control slide with PAS
Control Slides
staining kit, Cat. No. 101646
Control Slides with reference tissue for the Alcian blue staining in
ISOSLIDE™ Alcian blue
Control Slides
with Alcianblue-PAS, Cat. No. 101647 and 101646
Control Slides with reference tissue to verify standard hematoxylin and
eosin staining patterns in histological applications. These slides enable the
ISOSLIDE H&E
™
25 control slides 102755 clear detection of nuclei, epithelia, muscle and connective tissue elements.
Control Slides (NEW)
24 unstained paraffin sections, 1 stained control slide stained with 105174
Hematoxylin solution Gill III and 102439 Eosin-G solution.
ISOSLIDE™ Reticulin ISOSLIDE™ Iron Control Slides ISOSLIDE™ PAS Control Slides ISOSLIDE™ Alcian Blue
Control Slides Control Slides
ISOSLIDE™ H&E Control Slides
38
Control Slides with reference tissue for the detection of argent-affin structures
ISOSLIDE™ Methenamine
25 control slides 102473 in histological tissue, 24 unstained paraffin sections, 1 stained control slide
Control Slides
with Methenamine silver plating kit, Cat. No. 100820
Control Slides with reference tissue for the detection of Helicobacter pylori in
ISOSLIDE™ Warthin-Starry
25 control slides 102472 histological tissue, 24 unstained paraffin sections, 1 stained control slide with
Control Slides
Warthin-Starry siver plating kit modified, Cat. No. 102414
Control Slides with reference tissue for the detection of Mycobacteria in
ISOSLIDE™ Acid-fast bacteria
Control Slides
with TB-color modified Cat. No. 100497
Control Slides with reference tissue for the detection of amyloid in histological
ISOSLIDE™ Congo Red
25 control slides 102561 tissue, 24 unstained paraffin sections, 1 stained control slide with Congo red
Control Slides
staining acc. to Highman, Cat. No. 101641
ISOSLIDE™ Methenamine ISOSLIDE™ Warthin-Starry ISOSLIDE™ Acid-fast bacteria ISOSLIDE™ Congo Red, light
Control Slides Control Slides Control Slides microscopy
ISOSLIDE™ Congo Red,

polarised light
39
Other Products and Auxiliaries for Histology
Acetic acid (glacial) 100% 1 L, 2.5 L 100063 Additive for Bouin’s solution
Acetone 1 L, 2.5 L 100014 Dehydration
Mounting histological sections 10 % albumin-glycerol

Albumin (from bovine blood) 5 g, 25 g 112018
mixture [1+1]
Aluminum chloride 100 g, 500 g 801081 Mucicarmine staining
Aluminum hydroxide 1 kg 101091 Mucicarmine staining
Aniline, GR 250 mL, 1 L 101261 Azan staining
Aquatex® Mounting Medium 50 mL 108562 Aqueous mounting medium in cytochemistry
Calcium chloride dihydrate, GR 100 g, 500 g 102382 Fixing of tissue
DAB-buffer tablets 50 tablets 102924 Chromogene, non-aqueous specimen
DPX new 500 mL 100579 Permanent preparations
100 mL, 500 mL,

Entellan™ new Mounting Medium 107961 Mounting medium
1L
Permanent preparations especially for the application
Entellan™ new for cover slipper 500 mL 100869
with cover instruments
absolute, for analysis EMSURE®
Ethanol 1 L, 2.5 L 100983 ACS, ISO, Reag. Ph Eur
Dehydration agent
denatured with about 1% methyl ethyl
Ethanol 1 L, 2.5 L 100974 ketone for analysis EMSURE®
Dehydration agent
Contains calcium carbonate as acid binding basis;
Formaldehyde solution min. 37% free from acid 1 L, 2.5 L 103999
filter before use
GR for analysis stabilized with about 10% methanol
Formaldehyde solution about 37% 1 L, 2.5 L, 5 L, 25 L 104003
ACS,Reag. Ph Eur
Formaldehyde solution 4%, buffered, (pH 6.9) 5 L, 10 L 100496 Ready-to-use fixing solution
Tetrachloroauric(III) acid trihydrate 99% 1 g, 5 g 101582 Gold chloride solution
Histosec™ Paraffin Pastilles 4 x 2.5 kg, 25 kg 111609 Paraffin embedding
Histosec™ Paraffin Pastilles without DMSO 4 x 2.5 kg, 25 kg 115161 Paraffin embedding
Histosec™ Paraffin Pastilles 60 1 kg, 4 x 2.5 kg 101676 Paraffin embedding
Hydrogen peroxide 30% 108597
Hydroquinone 250 g, 1 kg 822333 Hydroquinone solutions
lodine 250 g, 500 g 104761 Weigert’s iodine solution
Manufactured according to the international standard ISO

8036. Lower toxicity.
Iron(III) chloride hexahydrate 100 g, 250 g 103943 Weigert’s solution
Kaiser’s glycerol 100 g 109242 Aqueous mounting agent
100 g
Kaiser’s glycerol gelatine, phenol-free (NEW) 108635 Aqueous mounting agent
(dropper bottle)
1-Propanol for analysis EMSURE® ACS, Reag. Ph Eur 1 L, 2.5 L 100997
40
Methenamine cryst., extra pure, USP 1 kg 104339 Additive for silver solution
M-FREEZE cryo-embedding medium (NEW) 100 mL 103693 Embedding medium for frozen sectioning.
Molybdatophosphoric acid hydrate 25 g, 100 g 100532 Trichrome staining
Neo-Clear™ Xylene Substitute 5 L, 25 L 109843 Xylene substitute
100 mL (dropper Non-aqueous mounting medium after Neo-Clear™

Neo-Mount™ Mounting Medium 109016
bottle), 500 mL Xylene Substitute
Bone-decalcification solution for bone and hard tissues
OSTEOMOLL® Decalcifier-Solution 1 L, 2.5 L 101736
in histology
Bone-decalcification solution for sensitive,
OSTEOSOFT® Decalcifier-Solution 1 L, 10 L 101728
calcium-containing tissue in histology
Periodic acid 25 g, 100 g 100524 PAS reaction
Potassium hexacyanoferrate(II) trihydrate 100 g, 500 g 104984 Iron staining
Potassium iodide 250 g, 500 g 105043 Weigert’s iodine solution
2-Propanol 1 L, 2.5 L 109634 Dehydration agent
Sodium thiosulfate pentahydrate 100 g, 500 g 106516 Sodium sulfate solutions
di-Sodium tetraborate 100 g, 250 g 106306 Additive for silver solution
3-(Triethoxysilyl)propylamine 100 mL 821619 Silanization 2% in acetone, 5 min
Tris buffer
Tris(hydroxymethyl)aminomethane 100 g, 500 g 108386
Tissue fixation
Tungstophosphoric acid hydrate 100 g, 250 g 100583 Additive for trichrome staining

Xylene (isomeric mixture) (NEW) 4L 108298 specifically for processing, deparaffination,
dehydration
Stomach biopsy, paraffin section,

Warthin Starry silver plating
41
Neo-Products
42
7
User-and Environmental-Friendly Product Line
The Neo-products were developed with the intention
of meeting the requirements of increased safety in
the laboratory as well as the increasingly stringent
regulations introduced by the environmental authorities.
Neo-Products are user- and environmentally friendly
products for use in medical laboratories. They are
designed to minimize the toxic, environment-damaging
and unpleasant properties of traditional laboratory
products such as fixatives and solvents. However, it is
important that the Neo-products should be able to be
used in the same way as conventional products; they
should also be suitable for routine use. Consumption
should be comparable and the results obtained must be
identical to those previously obtained. The Neo-product Neo-Clear™ xylene substitute is an aliphatic hydrocarbon
with a chain-length of C9-C11
range is new and will be constantly expanded to meet the
requirements of users.
Xylene substitute for histoprocessing

Neo-Clear™ Xylene Substitute 5 L, 25 L 109843
deparaffination, dehydration
100 mL (dropper bottle),
Neo-Mount™ Mounting Medium 109016 Mounting medium with Neo-Clear™ Xylene Substitute
500 mL
Histoprocessing Deparaffination Dehydration Mounting with

with Neo-Clear™ with Neo-Clear™ with Neo-Clear™ Neo-Mount™
xylene substitute Paraffin cuts xylene substitute Staining xylene substitute mounting medium
43
Neo-Products
Neo-Cytocolor™ Rapid Staining Kit
The Neo-Cytocolor™ rapid staining kit is suitable for all cytological sample material – gynecological as well as clinical.
In contrast to Cytocolor™ rapid staining kit, the clearing agent xylene is replaced by Neo-Clear™ xylene substitute,
making Neo-Cytocolor™ rapid staining kit safer for users and the environment. This alternative method of Papanicolaou
staining uses modified hematoxylin and polychrome solutions, and omits the orange staining step. Structures that are
normally stained with orange solution are stained using Eosin Y from the polychrome solution, and appear pink instead
of orange. The intensity of the pink color depends on the degree of keratinization of the cells. In approximately
4 minutes and 10 steps, Neo-Cytocolor™ rapid staining kit produces a stained slide with complete information on
integrity, hormonal status and bacterial condition. The kit has a capacity for up to 1,000 slides and a shelf life of 3
years. The Neo-Cytocolor™ rapid staining kit is suitable for small to medium-sized labs which stain cytological sample
material due to the simplification of the complex Papanicolaou protocol.
1 pack
Fast staining kit for a modified
500 mL modified hematoxylin solution Papanicolaou stain, with Neo-Clear™ Xylene
Neo-Cytocolor™ Rapid Staining Kit 500 mL modified polychrome solution 101971
Substitute
3 x 500 mL 2-propanol GR for clearing
Neo-Cytocolor™ rapid staining kit, smears

clinical and gynecological material
44
SNAP id® System for
Immunohistochemistry
8 45
Immunodetection Reagents and Instrument for IHC
SNAP id® 2.0 IHC

SNAP id® 2.0 Protein Detection System for Immunohistochemistry
(IHC) introduces a new capability to the innovative, vacuum-driven
SNAP id® 2.0 system. The IHC frame and slide holders allow you to
block, probe, and stain up to 12 tissue slides per frame. Reduced
handling time and multiple-slide processing make this system ideal
for antibody and protocol optimization.*

*For Research Use Only
Sample Prep Sample Mounting Antigen Recovery (if required) Blocking
Immunohistochemistry workﬂow Antibody Incubation SNAP id®

includes blocking, antibody incubations, 2.0 Steps
Labeling
labeling and wash steps, all of which can
be streamlined using the SNAP id® 2.0 Staining
Protein Detection System for IHC.
= Wash Mounting & Visualization
How does the SNAP id® 2.0 Protein Detection Key Benefits:
System for IHC work? • Eliminates the need for pap pens
• Antibodies can be collected and reused
With two individually controlled sides, the system base • Slide handling time is significantly decreased
allows for independent, vacuum-driven processing of • Less time spent on wash steps
either one or two IHC frames. Each of the IHC frames • Parallel processing of multiple slides
can process between 1 to 12 glass slides through
independent vacuum ports. Each slide holder has an Key Features:
injection/recovery port that enables the manual addition, • Flexibility of multiple slide configurations enables the
as well as the removal and recovery, of small volumes processing of 1 to 24 slides at a time
of antibodies or reagents; reagents can also be flushed • Compatible with standard IHC slides and protocols
using the vacuum feature if conservation is not a priority. • Compatible with diverse tissue preparations including
formalin-fixed or fresh frozen samples
• Intuitive format
SNAP id® 2.0 system – Base system* • Incorporates blocking, washing, and antibody
Product Catalog No. incubation and labeling steps
• Systematizes handling multiples slides without
SNAP id® 2.0 Protein Detection System – Single IHC SNAP2IHC the cost of automation
SNAP id® 2.0 Protein Detection System – Double IHC SNAP2IHC2
• Test tracker feature on frame cover helps keep track
of IHC steps
SNAP id® 2.0 consumables*
Product Size Catalog No.
SNAP id® 2.0 IHC Frame 1 EA SNAP2FRIHC
SNAP id® 2.0 IHC Slide Holders 24/pk SNAP2SH
46
Comparable Performance to Traditional Methods,
Even in Archival Tissue
The SNAP id® 2.0 IHC System produces comparable staining to traditional protocols even in archival tissue. In the
example below, the system was used to detect Aquaporin 1 in archival human kidney tissue. Note the characteristic
differential staining of kidney proximal tubule epithelium and renal corpuscle. Despite processing 12 slides in parallel
and shortening the handling time and protocol, the staining is robust and consistent with no blotchy artifacts that
sometimes plague autostainers. There is no apparent tissue degradation as compared with traditional protocols.
Classic histological stains such as hematoxylin can be applied using the same system.
Detection of Aquaporin 1 in human kidney

tissue (formalin-fixed and paraffin-embedded
(FFPE): SNAP id® 2.0 IHC System (sections 1
– 13) vs. Standard IHC protocol (section M1).
Fifteen tissue sections (5 μm) were assembled
on slides. Slides were rehydrated and antigen
retrieved (heat-induced epitope retrieval,
HIER) using a citrate antigen retrieval
solution.in a pressure cooker for 15 minutes
at 110°C. Thirteen slides were then processed
using the SNAP id® IHC system, and one was
processed using the manual protocol. The
primary antibody was Anti-Aquaporin 1
(Cat. No. AB2219, 1: 2,000).
Anti-Actin Antibody, clone C4 100 µL MAB1501
Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11 50 µg MAB348
Anti-Choline Acetyltransferase Antibody 500 µL AB144P
Anti-GAD67 Antibody, clone 1G10.2 100 µg MAB5406
Anti-Microtubule-Associated Protein 2 (MAP2) Antibody 100 µL AB5622
Anti-NeuN Antibody, clone A60 500 µg MAB377
Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody 100 µg AB5320
Anti-Olig-2 Antibody 100 µL AB9610
Anti-Sox9 Antibody 100 µg AB5535
Anti-Tyrosine Hydroxylase Antibody 100 µL AB152
47
Appendix
48
9
pH-Indicators for Titrations in Aqueous Media
Blood vessel, paraffin section,

Congo red staining acc. to Highman
polarised light for positive control
Product Size Catalog No. Uses/directions/pH range
Alizarin yellow GG 10 g 106776 pH 10.0–12.1 light yellow/brownish yellow
Alizarin red S sodium salt 25 g, 100 g 106279 pH 4.3–6.3 yellow/violet
Brilliant green 50 g 101310 pH 0.0–2.6 yellow/green
Bromochlorophenol blue 5g 103022 pH 3.0–4.5 yellow/purple
Bromocresol green 1 g, 5 g, 25 g 108121 pH 3.8–5.4 yellow/blue
Bromocresol purple 5 g, 25 g 103025 pH 5.2–6.8 yellow/purple
Bromophenol blue 5 g, 25 g 108122 pH 3.0–4.6 yellow/purple
Bromophenol blue sodium salt 5g 111746 pH 3.0–4.6 yellow/purple
Bromophenol red 5g 103023 pH 5.2–6.8 orange-yellow/purple
Bromothymol blue 5 g, 25 g 103026 pH 6.0–7.6 yellow/blue
Bromothymol blue sodium salt 10 g 101895 pH 6.0–7.6 yellow/blue
Bromoxylenol blue 5g 103033 pH 5.7–7.4 yellow/blue
Chinaldin red 5g 102282 pH 1.4–2.4 colorless/red
Chlorophenol red 5g 103024 pH 4.8–6.4 yellow/purple
Congo red 25 g, 100 g 101340 pH 3.0–5.2 blue violet/red-orange
In the acid range: pH 1.2–2.8 red/yellow in the alkaline range:

m-Cresol purple 1g 105228
pH 7.4–9.0 yellow/purple
In the acid range: pH 0.2–1.8 pink-red/yellow in the alkaline
Cresol red 5 g, 25 g 105225
range pH 7.0–8.8 orange/purple
Crystal violet 10 g 101408 pH 0.8–2.6 yellow/blue violet
4-Dimethylamino-azobenzene 10 g 103055 pH 2.9–4.0 red/yellowish orange
2,4-Dinitrophenol 25 g 103464 pH 2.8–4.7 colorless/yellow
Eosin B 25 g, 100 g 115934 pH 1.4–2.4 colorless/fluorescent pink
Eosin Y 25 g, 100 g 115935 pH 0.0–3.0 yellow/fluorescent green
Erythrosin B 10 g, 25 g 115936 pH 0.0–3.6 orange/red
Hematoxylin 5 g, 100 g 104302 pH 5.0–7.2 yellow/violet
49
Appendix
lndicator liquid with color scale 100 mL 109177 pH 0–5
Indicator liquid with color scale 100 mL, 1 L 109175 pH 4–10
lndicator liquid with color scale 100 mL 109176 pH 9–13
Methyl orange 25 g, 100 g 101322 pH 3.1–4.4 red/yellow-orange
Methyl orange solution 0.1% 250 mL, 1 L 101323 pH 3.1–4.4 red/yellow orange
Methyl red 25 g, 100 g 106076 pH 4.4–6.2 red/yellow-orange
Methyl red sodium salt 25 g, 100 g 106078 pH 4.4–6.2 red/yellow-orange
Mixed indicator 5 for ammonia titrations 250 mL 106130 pH 4.4–5.8 red-violet/green
1-Naphtholphthalein 1 g, 5 g 106246 pH 7.1–8.3 brownish/blue-green
Neutral red 25 g, 100 g 101369 pH 6.8–8.0 bluish-red/orange-yellow
3-Nitrophenol 5 g, 25 g 106794 pH 6.6–8.6 colorless/yellow-orange
4-Nitrophenol 25 g 106798 pH 5.4–7.5 colorless/yellow
2,2',2",4,4'-Pentamethoxy-triphenylcarbinol 1g 111473 pH 1.2–3.2 red-violet/colorless
Phenolphthalein 25 g, 100 g, 500 g 107233 pH 8.2–9.8 colorless/red-violet
Phenolphthalein solution 1% in ethanol 250 mL, 1 L 107227 pH 8.2–9.8 colorless/red-violet
Phenolphthalein solution 0.375% in methanol 250 mL, 1 L 107238 pH 8.2–9.8 colorless/red-violet
Phenol red 5 g, 25 g, 100 g 107241 pH 6.4–8.2 yellow/red
Phenol red sodium salt 5g 111748 pH 6.4–8.2 yellow/red
In the acid range: pH 1.2–2.8 red/yellow in the

Thymol blue 5 g, 25 g 108176
alkaline range: pH 8.0–9.6 yellow/blue
Thymolphthalein 5 g, 25 g, 100 g 108175 pH 9.3–10.5 colorless/blue
In the acid range: pH 1.2–2.8 red/yellow in the

p-Xylenol blue 1g 108682
alkaline range:pH 8.0–9.6 yellow/blue
50
pH-Indicator Strips and pH Indicator Papers
Non-bleeding special and universal pH indicators strips
Graduation 1 pH units: Universal indicator strips pH 0–14.0 100 strips 109535
Graduation 0.5 pH units: Acilit® indicator strips pH 0–6.0 100 strips 109531
Neutralit® indicator strips pH 5.0–10.0 100 strips 109533
Alkalit® indicator strips pH 7.5–14.0 100 strips 109532
Special indicator strips pH 2–9 100 strips 109584
Graduation 0.2–0.3 pH units: Special indicator strips pH 0–2.5 100 strips 109540
Special indicator strips pH 2.5–4.5 100 strips 109541
General Mixture-Rules
To calculate the ratios involved in mixing two solutions of the same substance with given concentrations (example
1) or one solution with pure solvent (example 2) in order to obtain a solution of desired concentration, the following
schematic can be used:
75 30 Example 1
Two solutions of concentration 75% and 30% are available; a solution of
60 concentration 60% is required.
Result
30 15 30 parts of the 75% solution must be mixed with 15 parts of the 30% solution.
96 60 Example 2
A solution of 96% and a pure solvent with 0% are available, a solution of 60%
is required.
60
Result
0 36 60 parts of the 96% solution must be mixed with 36 parts of the solvent.
The parts referred to are by weight if the content of the solutions is given in weight %; on the other hand, these are
volumes if volume % is indicated. The process can be adapted to preparing solutions of a certain density (extract the
weight % from the density table and insert in the equation).
51
Appendix
Buffer Solutions
TRIS-HCl-Buffer Phosphate Buffer (Sørensen)
Stock solution A: Tris, 0.2 M Stock solution A: Potassium dihydrogen phosphate, 0.1 M
(dissolve 21.03 g tris = tris (dissolve 13.61 g KH2PO4 in 1,000 ml
(hydroxymethyl)-aminomethane in Aqua dest.)
1,000 ml Aqua dest.)
Stock solution B: Di-sodium hydrogen phosphate, 0.1 M
Stock solution B: 0.1 N HCl (dissolve 17.8 g Na2HPO4 x 2 H2O or
35.81 g Na2HPO4 x 12 H2O in
Preparation of the buffer solution: mix 25 ml stock 1,000 ml Aqua dest.)
solution A with x ml stock solution B according to the list
above and make up with Aqua dest. to 100 ml. Preparation of the buffer solution: mix stock solution
A and B according to the list above.
pH-value Stock solution B pH-value Stock solution A Stock solution B
7.2 44.7 5.30 9.75 0.25
7.4 42.0 5.60 9.50 0.50
7.6 39.3 5.91 9.00 1.00
7.8 33.7 6.24 8.00 2.00
8.0 27.9 6.47 7.00 3.00
8.2 22.9 6.64 6.00 4.00
8.4 17.3 6.81 5.00 5.00
8.6 13.0 6.98 4.00 6.00
8.8 8.8 7.17 3.00 7.00
9.0 5.3 7.38 2.00 8.00
7.73 1.00 9.00
8.04 0.50 9.50
52
Cleaning Agents for Laboratory Equipment
Extran® laboratory cleaning agents are environmentally safe and do not interfere with biological treatment of waste
water. However, if such cleaning agents have been used for removing substances harmful to the environment, the
used agent must be placed in a container.
Extran® Cleaning Agents for Manual Washing
Type/Form Size Catalog No. Concentration pH-value Uses
Universal agent for severely contaminated items, even for

MA 01
2.5 L, 10 L 107555 2–5–20% 11.6–12.0 hard water up to 40 °d; also suitable for cleaning bench
alkaline
tops, tiles and floors, suitable for ultrasonic cleaning
Special agent for cleaning precision equipment made of
MA 02
2.5 L, 10 L 107553 2–5% 7.2–7.5 glass, quartz and sensitive metals, suitable for ultrasonic
neutral
cleaning
Universal agent for severely contaminated items,
MA 03 phosphate-free, hence no adverse ecological effects, add
2.5 L, 10 L 107550 2–5–20% 11.6–12.0
phosphate-free demineralized water to naturally hard water, suitable for
ultrasonic cleaning
Extran® Cleaning Agents for Machine Washing
Type/Form Size Catalog No. Concentration pH-value Uses
AP 11 2 kg, 10 kg, Gentle removal of residues, e.g. in analytical

107558 20–40 g 11.3
mildly alkaline; powder 25 kg laboratories, cleaning effect same as AP 14 liquid
AP 12 2 kg, 10 kg, Energetic cleaning agent, particularly for starch and protein
107563 20–40 g 12.3
alkaline; powder 25 kg residues cleaning effect, same as AP 15 liquid
AP 13
2 kg, 10 kg,
alkaline with detergents; 107565 20–40 g 12.2 Energetic cleaning agent, particularly for fatty residues
25 kg
powder
Gentle cleaning in machines with liquid dosing, e.g. in
AP 14 2.5 L, 10 L,
107573 30–50 ml 11.2 analytical laboratories, phosphate-free, hence no adverse
mildly alkaline; liquid 25 L
ecological effects, cleaning effect same as AP 11 powder
Energetic cleaning in machines with liquid dosing;
AP 15 2.5 L, 10 L,
107575 30–50 ml 12.2 phosphate-free, hence no adverse ecological effects;
alkaline; liquid 25 L
cleaning effect same as AP 12 powder
AP 21 Pre-wash for residues of carbonates, hydroxides, proteins,
2.5 L, 10 L,
acidic with phosphoric 107559 10–30 ml 2.0 amines, etc., as a final rinse with neutralising effect; also as
25 L
acid; liquid a gentle main wash; prevents build-up of calcium deposits
AP 22
2.5 L, 20 L, Gentle pre-wash or final rinse with neutralising effect;
acidic with citric acid; 107561 10–30 ml 3.0
25 L prevents build-up of calcium deposits
liquid
AP 31 Addition to washing water containing foaming residues, such
2.5 L 107560 1–3 ml 5.1
anti-foaming agent; liquid as proteins, fats, soaps, and any type of emulsifier
Medical and dental practices, and for instruments used in
AP 41
2 kg, 25 kg 107570 30 g 11.4 anaesthesis, removing saliva residues, mucus, blood, etc.;
enzymatic; powder
temperature 55–65°C
Chromosulfuric acid 1 L, 2.5 L 102499 Stubborn residues adhering to glass equipment
Extran® cleaning agents in renowned Merck quality are the key to reliable cleaning of your labware
Merck provides its high-quality Extran® cleaning agents for more than 25 years now.
The benefits to you:

Extran® cleaning agents cleans reliably without leaving any residues performed.
Extran® cleaning agents is free from scented materials, colorants, chlorine and toxic ingredients.
Its active ingredients are biodegradable, so environmental demands are met.
53
Appendix
References
• Bancroft J.D., Gamble M. • Lang G.

Theory and Practice of Histological Techniques 5th Ed., Churchill Histotechnik. Praxislehrbuch für die Biomedizinische Analytik Springer
Livingstone, UK, 2002 Wien New York, 2006
• Boon M.E., Drijver J.S. • Lillie R.D., Fullmer H.H.

Routine cytological staining techniques: Theoretical Background and Histopathological Technique and Practical Histochemistry, 4th Ed., Mc
Practice, Macmillan Ltd., Hamshire, 1986 Graw-Hill, New York, 1976
• Burstone M.S. • Lyon H.

Enzyme Histochemistry and its Application in the Study of Neoplasms, Theory and Strategy in Histochemistry: A guide to the Selection and
Academic Press, New York, London, 1962 Understanding of Techniques, Springer-Verlag, Berlin, 1991
• Clark G. • Mulisch M., Welsch U., Editor

Staining Procedures, 4th Ed., Williams & Wilkins, Baltimore/London, Romeis-Mikroskopische Technik, 18. Auflage, Spektrum Akademischer
1981 Verlag, 2010
• Huber H., Löffler H., Faber V. • Pearse A.G.E.

Methoden der diagnostischen Hämatologie, Springer-Verlag, Berlin, Histochemistry. Theoretical and Applied, 4th Ed., Churchill-Livingstone,
1994 Edinburgh, 1985
• Kiernan J.A. • Polak J.M., van Noorden S.

Histological & Histochemical Methods: Theory & Practice, 2nd Ed., Immunocytochemistry. Modern Methods and Applications, 2nd Ed.,
Pergamon Press, Oxford, 1990 Wright, Bristol, 1986
• Horobin R.W., Kiernan J.A. • Rathert P., Roth S.

Conn’s Biological Stains, 10th Ed., BIOS Scientific Publisher, Oxford, Urinzytologie: Praxis und Atlas, 3. Aktualisierte Auflage, Springer-
UK, 2002 Verlag, Berlin, 1995
• Saccomano G.
Diagnostic Pulmonary Cytology, Published by American Society of
Clinical Pathologists, Chicago, 1978
• Storch W.B.
Immunfluoreszenzfibel: Grundlagen und neue Anwendungen in der
klinischen Immunfluoreszenz, 2. neu bearbeitete und erweiterte
Auflage, Blackwell Wissenschafts-Verlag, Berlin, Wien, 1997
• Vacca L.L.
Laboratory Manual of Histochemistry, Raven Press, New York, 1985
54
Classified Register
A C
Acid fuchsin Certistain Dry Dyes
®
15, 19 Coomassie® Brilliant blue 17
Acridine orange Certistain® Dry Dyes 19 Cresyl violet (acetate) Certistain® Dry Dyes 15
AFB, ISOSLIDE™ Control Slides 39 Cytocolor™ Rapid Staining Kit 23, 26
Alcian blue 8GX Certistain® Dry Dyes 15

D
Alcian blue, ISOSLIDE™ Control Slides 38
DAB/3,3'-Diamino-benzidine-tetrahydrochloride 41
Alcian blue solution 36
DAPI/4',6-Diamidino-2-phenylindole-hydrochloride 17, 19
ALPA, LEUCOGNOST™ Leukemia Detection Kit 32
Decalcification solution 6
Amido black 10 B 17
DPX new 8, 28, 40
AP, LEUCOGNOST™ Leukemia Detection Kit 32
Aquatex® Mounting Medium 7, 32, 34, 40 E

Auramine O 17, 19 Elastica van Gieson kit 36
Auto-Hemacolor™ Rapid Staining Kit 27, 31 Elastin solution acc. to Weigert 37
Azure II 17 Entellan™ Mounting Medium 8, 28, 34, 40
Entellan™ new Mounting Medium 8, 28, 34, 40

B
Entellan™ new for cover slipper 8, 28, 40
Basic set, LEUCOGNOST™ Leukemia Detection Kit 32
Eosin B Certistain® Dry Dyes 15, 49
Brilliant blue/Coomassie® 17
Eosin Y Certistain® Dry Dyes 15, 49
Brilliant cresyl blue Certistain® Dry Dyes 15
Eosin Y 0.5% alcoholic 36
Brilliant cresyl blue solution 33
Eosin Y 1% alcoholic 36
Brilliant green Certistain® Dry Dyes 15
Eosin-Phloxin alcoholic 36
Brilliant green 17
Eosin Y 0.5% aqueous 36
Buffer tablets pH 6.4 acc. to Weise 9, 34
Erythrosine B Certistain® Dry Dyes 15, 49
EST, LEUCOGNOST™ Leukemia Detection Kit 32
Extran® Cleaning Agents 53
Buffer solutions 52
F
C
Fast Green FCF Certistain® Dry Dyes 15
Canada balsam 8
Fixative, LEUCOGNOST™ Leukemia Detection Kit 32
Carbol-fuchsin solution, Ziehl-Neelsen 22
Fluorescein 5-isothiocyanate/FITC I 17, 19
Carmine Certistain® Dry Dyes 15
Fluorescence dyes 19
Carminic acid 17
Formaldehyde solution 4 % buffered (pH 6.9) 4, 40
Certistain® Dry Dyes 15
Formaldehyde solution min. 37% 4, 40
Cleaning agents, Extran® 53
Fuchsin Certistain® Dry Dyes 15
Congo red, ISOSLIDE™ Control Slides 39
Congo red staining kit 37
Control slides, ISOSLIDE™ Control Slides 38, 39
55
Appendix
G K
Gelatine 7, 10, 24 Kaiser’s glycerol gelatine 7, 10, 40
Giemsa’s azure eosin methylene blue 17, 30 Kaiser’s glycerol gelatine, phenol-free 7, 10, 40
Giemsa’s azure eosin methylene blue solution 23, 27, 30, 36 Kit for DNA staining acc. to Feulgen 37
Gill's hematoxylin solution 36

L
Glutardialdehyde solution 25% 4
Lactophenol blue solution 23
Glycerol 7, 10
Leishman’s eosin methylene blue 18, 31
Gram’s solutions 21
Leishman’s eosin methylene blue solution 30
Gram-color staining set 21
LEUCOGNOST™ ALPA Leukemia Detection Kit 32
Gram-color mod., phenol-free 21
LEUCOGNOST™ EST Leukemia Detection Kit 32
H LEUCOGNOST™ Fixative Leukemia Detection Kit 32
Harris hematoxylin solution 26 LEUCOGNOST™ NASDCL new Leukemia Detection Kit 32
H&E, ISOSLIDE™ Control Slides 38 LEUCOGNOST™ PAS Leukemia Detection Kit 32
Hemacolor® Rapid Staining Kit 27, 30 LEUCOGNOST™ POX Leukemia Detection Kit 32
Hemacolor® single solutions 4, 27, 30 LEUCOGNOST™ AP Leukemia Detection Kit 32
HEMATOGNOST Fe™ Staining Kit 33, 37 Light green SF Certistain® Dry Dyes 15
Hematoxylin Certistain® Dry Dyes 15 Löffler’s methylene blue solution 23
Hematoxylin crystallin 17, 49 Lugol’s solution 21
Hematoxylin solutions 26, 27, 34, 36 Lugol’s solution stab. with PVP 21
Histosec™ Paraffin Pastilles 5, 7

M
Hydrochloric acid in ethanol 10, 22
Malachite green Certistain® Dry Dyes 15
I Malachite green (oxalate) 18
IHC System, SNAP id® 2.0 46 Malachite green (oxalate) solution, Tb-color 22
Immersion oil 8, 24, 28, 34, 40 Masson Goldner trichrome staining kit 37
Immersion oil, ISO 8036, Halogene - Free 8, 24, 28, 34, 40 May-Grünwald’s eosin methylene blue 18, 31
Indicators 49, 50 May-Grünwald’s eosin methylene blue solution 30
Iron, ISOSLIDE™ Control Slides 38 Mayer’s hemalum solution 36
ISOSLIDE™ Control Slides 38, 39 M-FIX® Spray Fixative 5, 28
ISOSLIDE™ AFB Control Slides 39 M-FREEZE cryo-embedding medium 7, 11, 41
ISOSLIDE™ Alcian blue Control Slides 38 M-GLAS® Glass Coverslip 8, 28
ISOSLIDE™ Congo red Control Slides 39 Methenamine, ISOSLIDE™ Control Slides 39
ISOSLIDE™ H&E Control Slides 38 Methenamine silver plating kit acc. to Gomori 37
ISOSLIDE™ Iron Control Slides 38 Methyl blue 15
ISOSLIDE™ Methenamine Control Slides 39 Methyl green Certistain® Dry Dyes 15
ISOSLIDE™ PAS Control Slides 38 Methylene blue Certistain® Dry Dyes 15
ISOSLIDE™ Reticulin Control Slides 38
ISOSLIDE™ Warthin-Starry Control Slides 39
56
N R
NASDCL, LEUCOGNOST™ Leukemia Detection Kit 32 Reticulin silver plating kit acc. to Gordon & Sweeets 37
Neo-Clear Xylene Substitute

™
11, 28, 41, 43 Reticulin, ISOSLIDE Control Slides
™
38
Neo-Mount™ Mounting Medium 11, 28, 41, 43 Rhodamine B 18, 19
Neutral red 17 Ringer’s tablets 12, 24
Neutral red Certistain® Dry Dyes 15, 50

S
New fuchsin Certistain Dry Dyes
®
15
Safranine O Certistain® Dry Dyes 16
Nigrosine Certistain® Dry Dyes 15
Schiff’s reagent 12, 34, 36
Nile blue Certistain® Dry Dyes 16
Schiff’s reagent Intense 34, 36
Nitro blue tetrazolium chloride 18
Shorr staining solution 27
Nuclear fast red Certistain® Dry Dyes 16
Silver plating kit acc. to von Kossa 37
Nuclear fast red-aluminum sulfate solution 0.1% 36
SNAP id® 2.0, IHC System 46
O Spray Fixative, M-FIX® 5, 28
Oil red O Certistain® Dry Dyes 16

T
Oil red O Solution 36
Tb-color 22
Orange G Certistain® Dry Dyes 16
Tb-color single solutions 22
Orange solutions 26
Tb-color modified 22
Orcein Certistain® Dry Dyes 16
Tb-Fluor 23
Osmium(VIII) oxide 5, 36
TB-fluor phenol-free 23, 37
Osmic acid solution 2% 5, 36
Toluidine blue O Certistain® Dry Dyes 16
OSTEOMOLL® Decalcifier-Solution 6, 41
Türk’s solution 33
OSTEOSOFT® Decalcifier-Solution 6, 41
Trypan blue 18
P
U V
Papanicolaou’s solutions 26
von Kossa, silver
UV lamp 24 24
plating kit
Paraffin 7, 8
Pararosaniline Certistain® Dry Dyes 16, 19 W

PAS, ISOSLICE® 38 Warthin-Starry, ISOSLIDE™ Control Slides 39
PAS, LEUCOGNOST Leukemia Detection Kit

™
32 Warthin-Starry silver plating kit 37
PAS staining kit 37 Weigert’s iron hematoxylin kit 37
Periodic acid solution 0.5% aqueous 36 Weise, buffer tablets 9, 34
Picrofuchsin solution acc. to van Gieson 36 Wright’s eosin methylene blue 18, 31
Phloxin B Certistain Dry Dyes

®
16 Wright’s eosin methylene blue solution 31
Polychrome solutions 26
X
Ponceau S Certistain® Dry Dyes 16
Xylene (isomeric mixture) 13, 41
POX, LEUCOGNOST™ Leukemia Detection Kit 32
Prussian-blue-reaction, HEMATOGNOST Fe™ Staining Kit 33, 37 Z

Pyronine G Certistain® Dry Dyes 16, 19 Ziehl-Neelsen carbol fuchsin solution 22
57
Merck KGaA
Frankfurter Strasse 250, 64293 Darmstadt, Germany
Phone 1 (800) 645 5476
Email nacustomerservice@merckgroup.com
www.merckmillipore.com
© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. Sigma-Aldrich Co., LLC is a subsidiary of Merck KGaA, Lit. No. 05018
Darmstadt, Germany. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via CM-17-06-0145
publicly accessible resources. 1/2018
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The life science business of Merck operates as

Insert sheets for all CE registered products are

Quality control is hence of the utmost

Prior to releasing the products for particular

 he performance of each product is also verified

The excellent reproducibility of the results for

2 Dyes for Microscopy

The term In Vitro Diagnostic Devices means any medical

• concerning a physiological or pathological state,

Classification rules of IVD directive:

CE certification is a self-certification process in which

Product Size Catalog No. Directions for use

Acetone 1 L, 2.5 L 100014 In conjunction with other fixatives

Chromium(IV) oxide, GR, ACS 250 g 100229 0.5–0.25% aqueous solution

absolute for analysis EMSURE® ACS, ISO, Reag. Ph Eur

Contains calcium carbonate as acid binding basis;

Fast staining with Hemacolor® rapid staining kitt for blood

Methanol 1 L, 2.5 L 106009 Fixation of blood smears

Intestine, paraffin section,

Product Size Catalog No. Directions for use

1–2% aqueous solution, several hours necessary to dissolve

1-Propanol for analysis EMSURE ACS,

1-Propanol, extra pure 1 L, 2.5 L 100996

2-Propanol (iso-propyl alcohol) for analysis

2-Propanol (iso-propyl alcohol) extra pure, USP 1 L, 2.5 L 100995

Trichloroacetic acid, GR, ACS 100 g, 250 g 100807 5–10% solution

Product Size Catalog No. Directions for use

Bone marrow, paraffin section,

Histosec™ Paraffin Pastilles

Product Size Catalog No. Directions for use

1 kg, 4 x 2.5 kg, 25 kg

M-FREEZE™ Cryo-Embedding Medium

Product Size Catalog No. Directions for use

Aqueous Mounting Media

Product Size Catalog No. Directions for use

Aqueous mounting agent, used for LEUCOGNOST™ leukemia detection kit

Gelatine 500 g 104070

A few crystals of thymol are added to avoid fungal contamination; nuclear

Non-aqueous Mounting Media

Product Size Catalog No. Directions for use

Canada balsam 25 mL, 100 mL 101691 Natural resin

DPX new 500 mL 100579 Non-aqueous mounting agent

Entellan™ Mounting Medium 500 mL 107960 Permanent slides (contains toluene)

Rapid mounting agent, permanent slides, no bubble

Mounting agent for the even coating of cytological smears

Paraffin liquid 100 mL, 500 mL 107161

Product Size Catalog No. Refractive index nD20°

Acetonitrile for analysis EMSURE ACS, ISO, Reag. Ph Eur

Acetonitrile, extra pure 1 L, 2.5 L 115500 1.345

Cinnamaldehyde 5 mL, 250 mL, 1 L 802505 1.622

1,2-Dibromoethane, for synthesis 5 mL, 250 mL, 1 L 800952 1.5368

Diiodomethane 100 mL 106053 1.740

Dinonylphthalate 100 mL 109669 1.486

1,2-Ethanedithiol, for synthesis 100 mL 800795 1.125

Ethylene glycol, extra pure 1 L, 2.5 L 100949 1.432

Product Size Catalog No. Directions for use

Acetic acid, glacial, 100%,

Acetic acid (glacial) 100% 1 L, 2.5 L 100063 Preparing stain solutions

Acetone 1 L, 2.5 L 100014 30–, 60–, 90–,100%, duration 15–20 minutes

Aluminum chloride hexahydrate, extra pure,

Ammonia solution, 25%, extra pure 1 L, 2.5 L 105422

he performance of each product is also verified