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L1 Introduction
L2 Chemical kinetics, Equilibrium binding, cooperativity
L3 Lambda phage
L4 Stability analysis
L5-6 Genetic switches
L7-9 E. coli chemotaxis
L10-11 Genetic oscillators
L12-13 Stochastic chemical kinetics
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II Systems Cell Biology (9 Lectures)
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III Systems Developmental Biology (2 Lectures)
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Main take home messages from this course:
‘Building an organism
starting from a single cell’
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MOVIE !
http://flymove.uni-muenster.de
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Pioneering experiments by Klaus Sander (1958)
on leaf-hoppers
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ligation and transplantation
experiments indicate the presence
of morphogens created/destroyed at
the poles of the embryo
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First morphogen:
bicoid (true maternal)
transplantation of bicoid
can rescue cells
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interpreting the bicoid gradient (created
by maternal effects) by zygotic effect
(gene expression by embryo itself)
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Image removed due to copyright considerations.
hunchback reads
the bicoid gradient
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recent experimental paper explores relation
between bicoid and hunchback quantitatively:
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How can you make a steep step in hunchback
exactly in the middle of the embryo from
a noisy bicoid gradient ?
Second example:
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remember robustness of chemotaxis (L9-10):
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explore robustness in Drosophila patterning
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main molecules of interest:
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simple reaction-diffusion model:
∂[ Sog ] ∂ 2 [ Sog ]
= DS − kb [ Sog ][ Scw] + k −b [ Sog − Scw] − α [Tld ][ Sog ]
∂t ∂x 2
∂[ Scw] ∂ 2 [ Scw]
= DBMP − kb [ Sog ][ Scw] + k −b [ Sog − Scw] + λ[Tld ][ Sog − Scw]
∂t ∂x 2
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robustness analysis
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∂[ Sog ] ∂ 2 [ Sog ]
= DS − kb [ Sog ][ Scw] + k −b [ Sog − Scw] − α [Tld ][ Sog ]
∂t ∂x 2
∂[ Scw] ∂ 2 [ Scw]
= DBMP − kb [ Sog ][ Scw] + k −b [ Sog − Scw] + λ[Tld ][ Sog − Scw]
∂t ∂x 2
∂ 2 [ Sog ]
0 = DS − kb [ Sog ][ Scw]
∂x 2
∂2 1 kb
0 = 0 − kb [ Sog ][ Scw] + λ[Tld ][ Sog − Scw] =
∂ 2 [ Sog − Scw] ∂x [ Scw] DS
2
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