URIC ACID FS* Toos Cat. No, Kitsz0 40300021 RIAX 20m + RZ1x _20mI + 1x Smistd 19001 99 10025 RISK 8Dmi+R21x 100ml 40300029 RIT GODmi+R21x 200ml 10300 030 6x Smi standard Method Enzymatic colorimetric test, "TOOS* Utic acid is oxidized to Allantoine by Uricase. The generated Hydrogen Peroxide reacts wih 4-Aminaantipyrine and N-Ethy NA2-nycroxy-3-sulfopropyl}¢m-tolucin (TOOS) toa blue violet dye. Reagent preparation and stability “The reagents and the standard are readysto-use and stable upto the ‘ond ofthe indicated month of expiry, if cantamination is avoided and Sslored at 2~ 8 °C. The reagents must be protected from light. Specimen ‘Serum, heparinized or EDTA plasma, urine. For serum / plasma Avoid hemolysis! Dilute urine + 10 with dit, water, Components and concentration in the test Rt: Phosphate buter pH7.0 100 mmalt Toos ‘mmol Ascorbate Oridase 21 KUM R2: Phosphate buter pH70 100 mmatt ‘Aminoantpyrine 0,3 mmol Potassiummhexacyanoterate (I) 10 mola Po 21 KU! Urcase 250 Un ‘Standard: 6 mola (357 umnolt) Notes “The reagents contain Sodium Azide (0095 %) as preservative. Do not swallow! Avoid contact wth skin and mucous membranes! Normal range ** rene) ‘Serum’ Men: 34-7.0mgldl 200-420 yo Women: 24-S.7mgidl 140 340 matt Urine: 250-750 mg!24n 1,5 4.5 mmoli24 n References 4. Barham, D., Trinder,P., Analyst. 97, (1972), 142 — 145, 2 Thotels, W., Hoffmeister, H., Busch, E.W., Koller, P.U., Volimar, J, Dtsch, Med. Wehr. 98, (1973), 980 386 3. Hem, Rd, Cannon, D.C, Winkelnann J: Clinical Chemisty, Principles and Technics, 2" edition, Harper & Row Publishors Ine, Hagerstown Marylend 1974, 534 4. Fossall,P., Prencipe, L., Bet G., Cin. Chem, 26, (1980) 22231 + fuid stable Targa BT 3000 INSTRUMENT PARAMETER Test Typ Endpoint with Starter ‘Serum Starter (Inactive) Filters (A/B) 546700 Units mg/dl Test Method With Factor Test Methodology Toos Number of washes an Delay Time (Sec) 0 Inc. Time (Sec) 300/300 Reading Time (Sec) 10 Test Limit (Conc) 20 Reactio Limit (mABS) 0.000 Max ABS Delta (mABS) 0.000 Reagent mAbs Limit (mABS) 100 Reagents A/B (ul) 240/60 Reaction Direction Increasing Reagent Dilution 1 Initial ABS. (maBs) 0.000 Curve Acceptance 100% ‘Automatic profile (Inactive) Rerun Test Rat Bik HM — 00:00 Pathological Repetition (Inactive) Serum Parameters Tests Name URIC ACID ‘Sample Volume ww 6 Dilution Ratio 18 Min, Max. M. 3.4017.00 Min: Max. F. 2.4015.70 Min. Max. B. (0.000/0.000 Urine Parameters Tests Name Urine Volume uy 8 Min, Max. Mt 0.000 / 0,000 Min: Max. F. 0.000 1 0.000 Min, Max. B. 0.000 1 0.000 Auto Dilution 410 Multi Factor 4,00 CALIBRATOR: 15.1097