Dr. Nancy C, Alombro —ar 213 NAME Tadgmalyn in jinang COURSE AND YEAR _BS P= 1 EXERCISE NO. 8 ACID-FAST STAINING This technique separates acid-fast organisms, e.g. members of Mycobacterium and some Actinomycetes from non acid-fast organisms. It is useful in diagnosis of tuberculosis. OBJECTIVES: 1. To apply the acid-fast procedure 2. To detect acid-fast reaction of bacteria MATERIALS: Acid-fast staining reagent: Carbolfuchsin, cultures; glass slides |-alcohol, alkaline methylene blue; bacterial PROCEDURES: . Prepare a smear and fix. 1 2. Flood the slide with carbolfuchsin for not less than 10 minutes. Wash the slide with tap water. 3. Wash gently with acid-alcohol until pink color disappears from thinner portion of the smear. Wash with tap water. . Dip the slide in alkaline methylene blue for | minute. Wash gently with tap water. . Blot-dry the slide. Examine under oil immersion objective. . Make labeled sketches of representative microorganisms seen, stating their morphology and staining characteristics. DRAWINGS: Ar Natural Science Department, College of Arts and Sciences (S¥ 2012-2013) Notre Dame University‘Nur 213 Dr. Nancy C. Alombro QUESTIONS: deaigr iaetion ad cance) be ciacelos 1. What is a differential stain? Name two examples of such stains. A differential erin Les 4wo cor vactir?|_ofyed fo liquns Tete dager types ty argent: Exaile irl tee S09 ABP Ace — farch Sain 2. Is gram stain an adequate substitute for an acid-fast stain? Why? s agin uot ain aden aiakblt UN wm Ogpricms ce Oi te clue Ui oe! in On afft fod ack | hackyia, Led eS | « ~~ s | -~ % | Kw | BG ‘Natural Science Department, College of Arts and Sciences (SY 2012-2013) Notre Dame University =25-