Lead (Pb) Bioaccumulation In Genera Bacillus

Name : Achmad Arifiyanto

NRP : 1509 100 062
Department : Biologi
Advisor Lecturer : Dr.rer.nat.Ir.Maya Shovitri, M.Si.

ABSTRACT

Lead (Pb) includes a group of large heavy metal in nature

were toxic either on animal or human and did not provide an
advantage functional biologically. Bacillus isolates S1 and
SS19 known resistant to lead up to 50 mg / L PbCl2. In this
research will be examined whether genera bacillus isolates S1
and SS19 can accumulate metal lead (Pb), their capability in
accumulating and profile protein differences when the
bacteria genera bacillus isolates S1 and SS19 get exposed
metal lead (Pb). Inoculum at age ± 9 hours are used, with a
Nutrient Broth (NB) containing 50, 75 and 100 mg / L PbCl 2.
Inductively Coupled Plasma Atomic Emission Spectrometry
(ICP) used to assessed Pb2+ concentrations. Bioaccumulation
levels of Pb2+ by Bacillus isolate S1 and SS19 related to the
distinction of beginning concentration to the final
concentration. Bacillus isolate S1 achieved 53% and 51%
bioaccumulation efficiency rate in lead presence
concentration (75 and 100 mg/L) and 51% (50 mg/L).
Another way Bacillus isolate SS19 able to accumulate 57%
(50 mg/L PbCl2) and kept stable on 36% bioaccumulation
efficiency rate (75 and 100 mg/L PbCl2). Regarding SDS-
PAGE electrophoresis protein profile result, protein in ± 127
kDa, molecule mass detected in the presence of Lead for
Bacillus isolate S1.

Key words : Lead, Bacillus isolate SS19, Bacillus isolate S1

and bioaccumulation

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