= HABITAML ALU Date gxpt. No 4 - ARBE1O304 Page No. [| Peet meet —4 | 4 71 ~ LNCis METHop (| Ata. Tol ote L fi vee. Mor ¢ otto d Atwolt Were Couttion T* [= ComM Gy ues Meisel ¢ PH Goh owe EDTA (PU ep), de-ionized Water Alea line (yor \ubtion TD > Geatocoy, — ot -On-2N ALLO sty 2b) SDs glo-iera ate zyo-cia| acettc—ocid = Ethows ot Cyly) Tito pywfanol r—. fl qeE-Ruase Phy QO Teacher's Signature Scanned with CamScannerthat loaypede DNA) otoi J Dvorlet (tuazyinos 4 Tradditi., PNarw ave also adcled to ceqracde Yo yelvared Prwtfa 7 | ty tte ce ysis aud tue yeleace | Kl Teacher's Signature Pm the ¢ eg \t z anael 14 ¢ ds Qure Sep ated p—pehewe gs tue plasual L DMA is citecule panecl of tao is ntewtC T ° ™ ° U i demotuyos the genomic amd Placard Dus HO Who ACS Ce ttl Pyp-teiv The yutaas PVA] Scanned with CamScannera f pape yew ins +0 Poleqicouy [a tevdr, clin a. wae Typ Fina oe weutantt Dotiow buf. | Jacotote i ¢ adde cl to woutya liz. ima Wig cait concentration that Lend . 7 Stvoimed Che Wo Shands A tcuspid vewoiun 4 sb) .2-A1a 2 ft ef Potaccritua| i Ae Sty 4 | | awatine Condittorc. ~ [eo The-addition of Potecciums Acetate rsuits | encists of Cpe Io de awd pmtein te - 1 ; ting emMactinw of «white ¢ iPitate haat | & |_| Patios of te gourtion | Al\lour¢ +4 Are activa tie of DMA. Tho large Boh So \ Ut Chunmuecm nal DA t > opie | iv tre Precip: pate a WADE AI SEN LL 2 OAL Cre Surarl | ended poy Cowtyifidation. & =| Tr pro cipitet? ancl clvenmomat DNA ts load nut te Cle wi MAet Folio wing Coysdvifidatine, the Sorbio. plasuaid LOND Gin lac Purcitiecl fren tue Soictinan by Teacher's Signature Scanned with CamScanner—“ a — ei —— ____Date { Expt. No. L8ERTo2 04 7 ———— — Pate No. L Metacdl_| => ([Meticd proc duce I : [41 PrMiJlat Byowa guituye tp d-Swal labe'ted lena tule, | 4 [| Z| Ce ti LAG Ate at th.oo Pua fi 4 wat [Bl Remove $e. giipeenatant fyeona tue tbe. iA = 7 + | Ipette diy ac pose 7 bie | Sl Adal deo 4L yebousportionn Lutte, yesusP ern of tae | pattlrial Pellet Pyspowl\4 hay Marte xi, Lig o ML Luce, ¢owttinas £0 at) hee! ws. vara pyechus paaele.) lete tte tbe Aght1y ad watt Combet eoeug nid 14 TwNeytivey +4 tiple 2-6 #Pine Laake tiie Cotte gang lot Come Neccioube dal Add 2 op file seiitra iZoti Oudout¢ planyo9y py p= Ueytiury tlie. tit Ab £iuaes, Lay enter woe ot tderp (Pa fey Sain La. | Lt 44 6 mwas Fert tp 2S idm | Volta CUP notowt ~zovki) aime z Min 4 pcpPvrPansy tte Wix re by jne/tus Lo, Teacher's Signature Scanned with CamScannerRRR TOSCO ie phere Fenn gin potete Vielows | To iselate Slo Prswid Dw A: \le Ino curLote Sm\\ of LR bywtn Eonteining, OLPOPVIODL anpiliotic S in culture ttUhe Wit bactetol colonies, Then we incwhute tnt coltere en a pte shaver win, deo rtm ot 27°C. Atte, that Ahe backevial e2il$ Poured into Tue conctyifege ef forcan trbt.and ea dant fuged Soy A minute at (LooerP + Thon tue pellet was formed, tne $UPevynratonrt por discarded aud mo Penet mx yetoineds how the f-e\ied Was VOUS Po,olg in drofar Of ice Cod Solution I ~ Srlution LT Contains Glucose, Tris Her J EOTA ~ BDTA Used 2 dhelate tre Mat I4soaghad Wirth +2 ubto “ons. 4 Meurboyeno4 Qua UbOHiires the pawibusy ane: Scanned with CamScannertet place al al yoon Cen eah v The sesuspen d per “4 Aov G minwtes> | | v Then the Freshy Pre pave d SotLCtvo ny i QooKkl Was added ine gh ef SPS and a tw 1) Contoun & L Lid v Solution twoct a ad wel by poer of o2N- Then we A aevtt V4 gent Pee eC mea (ce Vee Sov Lo winutes. Upon Vue a aldi tion ef Soi rtian TL Awe cau Ple becames Cltay because SDS Arnotured onetbive DW, Ah fren aot the Coturtion TH. @ dded ai BY TAaterting tube \ xd wet ave 4 On r wr 5 eG times Solution Til coppero® ae fared bi Baws ACLAALC yin Gyr evo | ace a dh Y Sojvrion (is added bo bviny bac obs | Oa pv ome ke Pye ciphlacthe OW | WWirlitet Poloteriw, ote od of Boackined Wes SBE sete. A woteste (een MON Mo tay iS Was {owt wis W * ee, Scanned with CamScanneroo —s © pitied at ates ey Then & the sovtin centni fuged at 20007) Aer & ends * | shonce aronster Ane superpohovt ints o | Y f Lvesh faaicon Aves 4 ae Soo ot ica cord WW sp fue tobe and mired we deep F222 for abort Lowinutt, | ey aud RNA are Precepitdted in this ad it wos Coucenrfuged 0 42,000 1Pes toy § wii. vy phe excess Uuid 4u6s bloted Evcto Clean | ecoprotave| aoldel, ond laced | £issul papery er Paper aa v The tener vinsed witr Jyat of qo ethanol dud ee cent ye at 42,0001 Are dowinutite Rewer tue cupey natonk Prd dM ea pejot fv 45 minutes, Piss eae ow he peu gn oo wero liter | 7 rt 0-4 XGE butter, @ uy hte tel ate wes Glen ned | v $o Se ane CHhaars), | ee Scanned with CamScannerLY vw 44ram of OGorrrte golcact -bo 400 TBE buffey pod meted tn 4 he NeW ened gaan tHe ef PET BR, Ate oF thet tne seutorn Poured ints he PUAS and it yooee allowed “Co Seaelify, MAY 9 c Ww Coy | Vv AYXGE budter prepored arvel powreel and tne Gei tAnE, then CONRFUIIY remolld tig Comb ourel e [nk ; Placed the Plate ingicle M2 Jal 7 The : im lon Gunmrt of po ee, Jon 14 Five weil followed a tia DNA Saw lt Ate wert two Weis poay Awadecs and The Gel os allowed +® yun, v - | On ComPourt wo, ak ladder, the 612 | | et Plasmid is C%ual fo 4kb Puede apereiwas | a eee Scanned with CamScanner