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ABC from Proteus vulgaris BSA free, lyophilized powder, 50250 units/mg protein (using chondroitin sulfate C as substrate) | SigmaAldrich
India Home C3667 Chondroitinase ABC from Proteus vulgaris
C3667 SIGMA
Chondroitinase ABC from Proteus vulgaris
BSA free, lyophilized powder, 50250 units/mg protein (using chondroitin sulfate C as
Attention:
substrate)
SigmaAldrich.com will be down for scheduled maintenance Saturday, August 20, 2016 at 8:00 am (08:00) Central Daylight Time U.S.
Synonym: Chondroitin ABC Lyase
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SDS SIMILAR PRODUCTS inconvenience.
MDL number MFCD00130795
POPULAR DOCUMENTS: DATASHEET (PDF) | SPECIFICATION SHEET (PDF)
Properties
Related Categories 4.2.x.x CO bonds , 4.x.x.x Lyases , Application Index ,
Biochemicals and Reagents , Carbohydrate Hydrolysis ,
More...
Quality Level PREMIUM
form lyophilized powder
composition Protein, ~10% Lowry
solubility 0.01% bovine serum albumin aqueous (BSA)
solution: soluble
Featured Industry Diagnostic Assay Manufacturing
foreign activity protease, essentially free
storage temp. −20°C
Description
Other Notes
Contains potassium phosphate buffer salts and stabilizer
View more information on enzymes for complex carbohydrate analysis at www.sigmaaldrich.com/enzymeexplorer
Packaging
5, 10 units in glass bottle
Packages based on chondroitinase C activity.
Preparation Note
Affinity purified
Unit Definition
One unit will liberate 1.0 μmole of 2acetamido2deoxy3O(βDgluc4enepyranosyluronic acid)4OsulfoDgalactose from chondroitin sulfate A or 1.0
μmole of 2acetamido2deoxy3O(βDgluc4enepyranosyluronic acid)6OsulfoDgalactose from chondroitin sulfate C per min at pH 8.0 at 37 °C.
Application
The enzyme has been used to study the consequences of inducing acute, longlasting changes in chondroitin sulfate proteoglycans using adult rat brain cells.
[2] Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from
fresh bovine corneal stromae.[1]
Biochem/physiol Actions
Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (14)βDhexosaminyl and (13)βDglucuronosyl or (13)αL
iduronosyl linkages to disaccharides containing 4deoxyβDgluc4enuronosyl groups. It acts on chondroitin 4sulfate, chondroitin 6sulfate, and dermatan
sulfate, and acts slowly on hyaluronate. The molecular weight is found to be approximately 120 kDa with 2 nonidentical subunits of molecular masses 86 kDa
and 32 kDa. The pH optimum is found to be 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid and temperature optimum is 37 °C. 1 mM Zn2+ acts as an
inhibitor, while 0.05 M acetate is an activator of the enzyme.
Price and Availability
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http://www.sigmaaldrich.com/catalog/product/sigma/c3667?lang=en®ion=IN 1/3
8/19/2016 Chondroitinase ABC from Proteus vulgaris BSA free, lyophilized powder, 50250 units/mg protein (using chondroitin sulfate C as substrate) | SigmaAldrich
albumin, ≥100 units/mg protein albumin, lyophilized powder,
(enzyme + BSA) ≥100 units/mg protein (enzyme +
BSA)
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Specification Sheet C3667 Datasheet (83 KB)
Carbohydrate Analysis bf8 (1 MB ) Enzyme Explorer
Articles
Glycosaminoglycan Sulfation and Signaling
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
Vicki Caligur
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System,
Chromatography, Coagulation, Degradations, Enzymelinked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, Help, High performance liquid chromatography,
Inflammation, Ionexchange chromatography, Ligands, Mass spectrometry, Methods, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain
reaction, Polymerization reactions, Proteomics, Reductive aminations, Sequences, Spectroscopy, Sulfations, Transcription, Type, transformation
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PeerReviewed Papers
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http://www.sigmaaldrich.com/catalog/product/sigma/c3667?lang=en®ion=IN 2/3
8/19/2016 Chondroitinase ABC from Proteus vulgaris BSA free, lyophilized powder, 50250 units/mg protein (using chondroitin sulfate C as substrate) | SigmaAldrich
read abstract
1. Keratocyte phenotype mediates proteoglycan structure: a role for fibroblasts in corneal fibrosis.
James L Funderburgh et. al
Journal of Biological Chemistry, 278(46), 4562945637 (2003)
In pathological corneas, accumulation of fibrotic extracellular matrix is characterized by proteoglycans with altered glycosaminoglycans that contribute to the reduced transparency of scarred tissue. During
wound healing, keratocytes in the corneal ...Read More
read abstract
2. Acute and longlasting changes in extracellularmatrix chondroitinsulphate proteoglycans induced by injection of chondroitinase ABC in the adult rat brain.
G Brückner et. al
Experimental Brain Research, 121(3), 300310 (1998)
Latticelike perineuronal accumulations of extracellularmatrix proteoglycans have been shown to develop during postnatal maturation and to persist throughout life as perineuronal nets (PNs) in many brain
regions. However, the dynamics of their reor...Read More
read abstract
3. Contrast enhanced computed tomography can predict the glycosaminoglycan content and biomechanical properties of articular cartilage.
P N Bansal et. al
Osteoarthritis and Cartilage, 18(2), 184191 (2010)
An early hallmark of osteoarthritis (OA) is the progressive loss of glycosaminoglycans (GAGs), the extracellular matrix (ECM) component of articular cartilage that confers it with compressive stiffness. Our aim
in this work is to establish the feasi...Read More
read abstract
4. A 4deoxy analogue of NacetylDglucosamine inhibits heparan sulphate expression and growth factor binding in vitro.
Xander M R van Wijk et. al
Experimental Cell Research, 316(15), 25042512 (2010)
Heparan sulphate (HS) is a long, linear polysaccharide, which has a basic backbone of beta14GlcAalpha14GlcNAc units. The involvement of HS in many steps of tumourigenesis, including growth and
angiogenesis, makes it an appealing target for canc...Read More
read abstract
5. Focal experimental injury leads to widespread gene expression and histologic changes in equine flexor tendons.
Else Jacobson et. al
PLoS ONE, 10(4), e0122220 (2015)
It is not known how extensively a localised flexor tendon injury affects the entire tendon. This study examined the extent of and relationship between histopathologic and gene expression changes in equine
superficial digital flexor tendon after a su...Read More
read abstract
6. The biological basis of degenerative disc disease: proteomic and biomechanical analysis of the canine intervertebral disc.
William Mark Erwin et. al
Arthritis research & therapy, 17(1), 240 (2015)
In the present study, we sought to quantify and contrast the secretome and biomechanical properties of the nonchondrodystrophic (NCD) and chondrodystrophic (CD) canine intervertebral disc (IVD) nucleus
pulposus (NP). We used iTRAQ proteomic methods...Read More
read abstract
7. Extracellularmatrixbased and ArgGlyAspmodified photopolymerizing hydrogels for cartilage tissue engineering.
Hwan D Kim et. al
Tissue Engineering, Part A: Tissue Engineering, 21(34), 757766 (2015)
Articular cartilage damage is a persistent and increasing problem with the aging population. Strategies to achieve complete repair or functional restoration remain a challenge. Photopolymerizingbased
hydrogels have long received an attention in the...Read More
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8. Soluble factors from the notochordalrich intervertebral disc inhibit endothelial cell invasion and vessel formation in the presence and absence of proinflammatory cytokines.
M C Cornejo et. al
Osteoarthritis and Cartilage, 23(3), 487496 (2015)
Chronic low back pain can be associated with the pathological ingrowth of blood vessels and nerves into intervertebral discs (IVDs). The notochord patterns the IVD during development and is a source of anti
angiogenic soluble factors such as Noggin ...Read More
read abstract
9. Perineuronal net degradation in epilepsy.
Elyse K RankinGee et. al
Epilepsia, 56(7), 11241133 (2015)
We previously reported loss of perineuronal net (PN) immunohistochemical staining around parvalbuminpositive interneurons in the hippocampus of rats after an episode of status epilepticus (SE). We
hypothesized that the loss of the PN could alter se...Read More
read abstract
10. Loss of corneal epithelial heparan sulfate leads to corneal degeneration and impaired wound healing.
Vivien Jane CoulsonThomas et. al
Investigative Ophthalmology & Visual Science, 56(5), 30043014 (2015)
Heparan sulfate (HS) is a highly modified glycosaminoglycan (GAG) bound to a core protein to form heparan sulfate proteoglycans (HSPGs) that are vital in many cellular processes ranging from development
to adult physiology, as well as in disease, th...Read More
read abstract
11. Abnormalities in Expression of Structural, Barrier and Differentiation Related Proteins, and Chondroitin Sulfate in Feline and Human Interstitial Cystitis.
Paul J Hauser et. al
The Journal of Urology, 194(2), 571577 (2015)
We analyzed the urothelium of cats diagnosed with feline interstitial cystitis to determine whether abnormalities in protein expression patterns could be detected and whether the expression pattern was similar to
that in patients with human intersti...Read More
read abstract
12. Ttype calcium channels contribute to calcium disturbances in brain during hyponatremia.
John Odackal et. al
Experimental Neurology, 273, 105113 (2015)
Disturbance of calcium homeostasis is implicated in the normal process of aging and brain pathology prevalent in the elderly such as Alzheimer's, Parkinson's, and amyotrophic lateral sclerosis. Previous
studies demonstrated that applying a hyponatre...Read More
read abstract
13. Chondroitin Sulfate Induces Depression of Synaptic Transmission and Modulation of Neuronal Plasticity in Rat Hippocampal Slices.
Elisa Albiñana et. al
Neural plasticity, 2015, 463854 (2015)
It is currently known that in CNS the extracellular matrix is involved in synaptic stabilization and limitation of synaptic plasticity. However, it has been reported that the treatment with chondroitinase following
injury allows the formation of ne...Read More
read abstract
14. Glypican5 stimulates rhabdomyosarcoma cell proliferation by activating Hedgehog signaling.
Fuchuan Li et. al
Journal of Cell Biology, 192(4), 691704 (2011)
Glypican5 (GPC5) is one of the six members of the glypican family. It has been previously reported that GPC5 stimulates the proliferation of rhabdomyosarcoma cells. In this study, we show that this
stimulatory activity of GPC5 is a result of its ab...Read More
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15. The progenitor cell marker NG2/MPG promotes chemoresistance by activation of integrindependent PI3K/Akt signaling.
M Chekenya et. al
Oncogene, 27(39), 51825194 (2008)
Chemoresistance represents a major problem in the treatment of many malignancies. Overcoming this obstacle will require improved understanding of the mechanisms responsible for this phenomenon. The
progenitor cell marker NG2/melanoma proteoglycan (M...Read More
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